NovaSeq Product Brochure - Illumina
We set out to create a sequencer with you in mind. One where youwon’t have to limit the scope or scale of your research. We built it withgroundbreaking innovations integrated with our proven sequencingtechnology. Crafted it to open new possibilities. Made it scalableand flexible for virtually any genome, method, and scale of project.And gave it the ability to sequence more economically than everbefore. The future of genomics is here.It starts with NovaSeq.
THE SEQUENCERYOU’VE BEENWAITING FORThe NovaSeq 6000 Sequencing System redefines what’s possible in high-throughputsequencing—with scalability and speed that allow you to bring new scientific visions to life.Now, you can use the highest levels of throughput more economically than ever before.Built with groundbreaking technological innovations that leverage our proven sequencingtechnology, you can choose the configuration that best suits your ever-evolving needs.With the highest throughput of any production platform, the NovaSeq 6000 System enablesyou to power studies with more samples and higher depth of coverage. Plus, with rapidturnaround capabilities available, you now have the ability to get to answers sooner.
SEQUENCING LIKE NO OTHERUsers can run 1 or 2 flow cells at a time, using any combination of the available read length and flow cell type.Provides a quick, powerful, and cost-effectiveoption for high-throughput applicationsSP 0.8 BFlow cell typeSingle reads*80 Gb2 x 50 output250 Gb 400 Gb2 x 150 output2 x 250 outputS1 1.6 B 167 Gb 333 Gb 500 GbFlow cell typeSingle reads*2 x 50 output2 x 100 output2 x 150 output*Clusters passing filterYields unprecedented throughput whileenabling cost-effective sequencing across a rangeof applications and depth of coverageS2 4.1 B417 Gb 833 Gb 1250 GbS4 10 B2000 Gb 3000 GbFlow cell typeFlow cell typeSingle reads*Single reads*2 x 50 output2 x 100 output2 x 100 output2 x 150 output2 x 150 output
ONE PLATFORM.INFINITEPOSSIBILITIES.Whether you are interested in high-output applications like whole-genomesequencing (WGS), looking to complete multi-omics studies, or simplyoperating more cost effectively, the NovaSeq 6000 System empowers youwith new possibilities.The platform is enabled by the broad portfolio of Illumina library preparation kits,providing the flexibility to run your applications at virtually any scale from single cellsto an entire population. The NovaSeq 6000 System also supports single-read andpaired-end sequencing, with read lengths of up to 2 250 bp—allowing you toadapt the run to meet your study’s specific needs. Whether exploring the genome,epigenome, or transcriptome, the possibilities for your research are endless.
A COMPREHENSIVESEQUENCING ECOSYSTEMThe NovaSeq 6000 System is not only our highest-throughput sequencer, but it is also our most intuitive and easy-to-usehigh-throughput platform, enabling a streamlined workflow across a broad range of applications.1MANAGE WORKFLOWwith BaseSpace Clarity LIMS or your existinglaboratory information management system(LIMS). The NovaSeq 6000 System is fullysupported out of the box with BaseSpaceClarity LIMS while also providing key integrationpoints for a wide variety of third-party LIMS.2PREPARE LIBRARIESwith a broad range of high-performancelibrary preparation kits from Illumina.Additionally, the Illumina QualifiedMethods program enables easy-to-accessautomation for key methods.3SEQUENCEprepared libraries on the NovaSeq 6000System. Get data quality you can trustwith proven sequencing by synthesis(SBS) chemistry, more than a decade inthe making.4ANALYZE DATAwith integration into BaseSpaceSequence Hub. Over 70 open-sourceand commercial bioinformatics appssupport a broad range of experimenttypes. Share your results in thesecure cloud environment.5INTERPRET RESULTSand get the most out of yourdata, driving scientific discoverywith BaseSpace VariantInterpreter and BaseSpaceCohort Analyzer.
“The NovaSeq System givescustomers access to power,breadth, and flexibility. This willignite a whole new set ofdiscoveries.”FRANCIS DESOUZA, CEO, ILLUMINAACCELERATINGBREAKTHROUGHS.ENABLING BETTEROUTCOMES.The NovaSeq 6000 System propels unprecedented progress in genomics—providing theflexibility and scalability to enable comprehensive research studies like never before—fromhigh-resolution single-cell studies to tumor-normal profiles, developing liquid biopsies, andpopulation-scale sequencing.Together, we can accelerate significant breakthroughs that will increase our understandingof genetic factors underlying the onset and course of disease, resulting in better treatmentsand improved patient outcomes. Ultimately, we won’t just react to illness—we’ll optimizeour wellness. The NovaSeq 6000 System gets us one step closer to that reality.
THE POWERIS YOURSRealize your scientific visions and shape the future of genomics with theNovaSeq 6000 System. Providing unprecedented scalability and flexibility,the groundbreaking innovation behind the NovaSeq System charts thepath toward broader, more comprehensive studies—enabling you todiscover more than ever before.
Explore the NovaSeq 6000 System at www.illumina.com/NovaSeqFor Research Use Only. Not for use in diagnostic procedures. 2019 Illumina, Inc. All rights reserved. PUB 770-2016-026 QB 5611
NovaSeq Series Brochure Author: Illumina Subject: The NovaSeq Series offers scalable throughput and flexibility for virtually any genome, sequencing method, and
Illumina Adapter Sequences Document # 1000000002694 v00 1 October 2015 Illumina Adapter Sequences. This document provides the nucle
The mRNA library was prepared using an Illumina Truseq Stranded mRNA High Throughput Prep kit and samples were sequenced using an Illumina NextSeq 500 Mid-Output Sequencing Reagent kit (v2, 150 cycles), 132 M reads on an Illumina NextSeq 5
From library prep, arrays, and sequencing to informatics, Illumina genomic solutions empower researchers and clinical researchers across the globe to find the answers they seek. When you join the Illumina community, you become part of a dynamic scientific movement that includes thousands of researchers and industry thought leaders.
sequencing. By modifying the primer constructs to include the Illumina adapters and a unique barcode, the amplified region of the 16S rRNA gene can be identified in a pooled sample and the sample is prepared for sequencing with the Illumina MiSeq platform. Figure 2. Protocol for barcoded Illumina sequencing. A target gene is identified, which .
Plus 24 library prep. plexWell libraries are compatible with the Illumina MiSeq, NextSeq, HiSeq and NovaSeq systems.1 The plexWell Plus 24 kit accommodates smaller batch sizes providing extra flexibility for those . plexWell Plus 24 Libra
SureSelectQXT Target Enrichment for Illumina Multiplexed Sequencing 3 Safety Notices CAUTION A CAUTION notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly performed or adhered to, could result in damage to the product or loss of important data.
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This standard employs the principles of API 650; however, storage tank owner/operators, based on consideration of specific construction and operating details, may apply this standard to any steel tank constructed in accordance with a tank specification. This standard is intended for use by organizations that maintain or have access to engineering and inspection personnel technically trained .
