Formulation And Evaluation Of Aloevera And Dacus Carota Herbal Cream
E-ISSN 2250-0944ISSN 2250-1150doi: 10.31838/ijprt/10.01.07Research ArticleFormulation and Evaluation of Aloevera and DacusCarota herbal creamSHARMILA DUSI, J.SAMINATHAN*Delhi Pharmaceutical Sciences and Research University, M.B Road, Sec – 3, PushpVihar, New Delhi*Corresponding AuthorEmail ID: swamilingam@gmail.comReceived: 25.05.19, Revised: 06.06.19, Accepted: 12.08.19ABSTRACTHerbal cosmetics are becoming common in the field of beauty, fashion. The present study is to formulate andevaluate the herbal cream containing extracts of natural products such as Aloe Vera, Cucumis Sativus andDaucus Carota. Different types of oil in water (O/W) herbal creams are preparing by changing concentrationsof ingredients. The evaluations of all formulations were done on different parameters like pH; viscosity,spreadibilty, and stability were examined. Formulations should not show any adverse effects. The formulationshows no redness, edema, inflammation, and irritation during irritancy studies. These formulations are safe touse for skin. These studies suggest that the composition of extracts and base of cream are more stable andsafe, it may produce synergistic action.Keywords: Herbal Cream, O/W emulsion, pH, Synergistic action, Cucumis Sativus, Dacus Carota.INTRODUCTIONBeauty, the quality that gives pleasure to thesenses, is perhaps the desire of every humanbeing on earth. Some are born beautiful and someare made beautifully [1]. Aesthetic appearance asalways been a matter of prime importance. Theword “Beauty” is not only related to human, as isoften though, but men also use cosmetic products[2]. Cosmetics are using in different forms toincrease their beauty [5].The word “cosmetics” arises from the Greek word“cosmetics” which means to adorn [6]. Materialsused to enhance their beauty are known ascosmetics. The methods are implementing toimprove beauty from past olden days.Cosmetics are the products, maintains contactswith the outer parts of the body without causingany harmful effect. Also, maintain good textureand appearance to the skin and also protects fromUV rays [8]. Cosmetics are used for removing dirtand maintaining good appearance withoutdisturbing our body functions. All skin creams,lotions, shampoos come under cosmetic productsonly [10].HERBAL COSMETICSMore herbal ingredients are used to providedefined cosmetic benefits only, the shell is called“Herbal cosmetics” the demand for herbalmedicines is increasing rapidly due to their lack ofside effects. The herbal cosmetic is that it is purelymade by herbs and shrubs [18]. The herbsextracted from nature do not show adverse effectson human skin. Now a day’s cosmetics are used toimprove their appearance. Cosmetics arepreparing and using to improve their beauty [11].For various types of skin ailments formulations likeskin protective, sunscreen, anti-acne, anti-wrinkle,either natural or synthetic.The development process for cosmetic formulationneeds maintenance of quality standards [9]. Theherbs used in cosmetic preparations have varietiesof properties like antioxidant, anti-inflammatory,antiseptic and anti-bacterial, etc. herbal productshaving no side effects compared with syntheticformulations [15].Benefits of Herbal Cosmetics Being natural, least harmful effect on the skinor other body parts. Relatively more safe. More placebo effect to the consumers due toits use in traditions and culture. Flexibility in formulation. Population proves effects from ancient time. Easy availability. Economical. It helps to cleans and beautify the bodywithout side effects. It normalizes the body functions. It has extreme nutritional value with highcontent of vitamins and minerals. It enhances the energy level of body [16]. It stimulates the body’s immune systemwithout disturbing the natural balance of thebody. Variety of Phyto-constituents can beincorporated.31 International Journal of Pharmacy Research & Technology Jan – Jun 2020 Vol 10 Issue 1
J.Saminathan et al / Formulation and evaluation of aloevera and dacus carota herbal creamMATERIALS AND METHODSTable 1: List of materials and their suppliersused in the present earic Acid5.Cetyl alcohol6.Almond IERNuzen Labs Pvt. Ltd,HyderabadSri Uma Export &Import, Alur, A.PFisherScientifics,MumbaiQualigenFineChemicals, MumbaiLobalChemiPvt.Ltd,MumbaiDabur Pvt. Ltd, NewDelhiSpecialistiesPvt.Ltd, MumbaiS. D. Fine - Chem,Ltd, MumbaiQualigenFineChemicals, MumbaiMETHODOLOGYPreparation of Extract Air-dried and coarsely powdered (500gm) of Aloe Vera and Daucus Carotawere placed in a Soxlet extractorseparately, using petroleum ether andthen successively with ethanol. The extracts were then concentrated todryness under reduced pressure andcontrolled Temperature, respectively andthey were preserved in a refrigerator.Cream Formulation: Oil in water (O/W) emulsion, (semisolidformulation) is formulated. The stearic acid cetyl alcohol and almondoil are dissolved in the oil phase andthese are heated To 75 C. this is part A. The water-soluble components likemethylparaben,triethanolamine,propylparaben, Aloe Vera extract andDacus Carota are dissolved in an aqueousphase and it is heatedup to 75 C. This is Part B. After heating, the aqueous phase wasadded in portions to the oil phase withContinuous stirring until the cooling of theemulsifier took place. The formula for the cream is given in tableTable 2: Formula for the creamNO1.INGREDIENTS3.Ethanol extractAloeveraEthanol extractDacus CarotaStearic acid4.5.6.7.8.9.Cetyl alcoholAlmond oilGlycerolMethyl LUATIONEvaluation of pH of the Cream:By using a buffer solution, the pH meter wascalibrated. The 0.5 g of the cream was weighedand dissolved in 50.0 ml of distilled water afterthat pH is measured.Dye test:The red dye is mixed with the prepared cream.Place a drop of the cream on a microscopic slidecovers it with a cover slip, and examines it under amicroscope. If the globules appear in red color,then the background is Colorless. The cream isknown as o/w type. If the condition is reversed,then it is known as w/o type cream i.e. thedisperse globules appear Colorless but thebackground is in red color.Homogeneity:The Homogeneity of a prepared formulation istested by touch and by appearance.Appearance:The appearance of the cream was judged by itscolor, pearlescence, and roughness and graded.After feel:The emollient nature and smoothness are checkedafter application.Type of smear:The formation of smears after applying to skin ischecked.Removal:The applied cream was observed for removal byusing tap water.Acid value:Take 10 gm of cream and dissolved in 50 mlmixture of equal volume of alcohol and solventether, then the flask was connected to refluxcondenser and heated, until the content wasdissolved completely, then add 1 ml of32 International Journal of Pharmacy Research & Technology Jan – Jun 2020 Vol 10 Issue 1
J.Saminathan et al / Formulation and evaluation of aloevera and dacus carota herbal creamphenolphthalein and it is titrated with 0.1NNaOH, until light pink color appears after shakingthe flask for 30 seconds[21].Acid value n 5.61/wn amount of NaOH required.w the weight of the substance.Saponification value:Introduce about 2 gm of substance refluxed with25 ml of 0.5 N alcoholic KOH for 30 minutes, tothis 1ml of phenolphthalein added and titratedimmediately, with 0.5N HCL.Saponification value (b-a) 28.05/wThe volume in ml of titrant aThe volume in ml of titrating bThe weight of the substance in gm wIrritancy test:Apply prepared cream on the backside of the lefthand. Then the area of cream applied and time istaken into consideration. Irritation on appliedarea, eczema, other rashes are observed within24 hrs after the application of a cream [20].Accelerated stability testing:Accelerated stability testing is performed for morestable two prepared formulations. Stability testingis performed at least for one week. Theformulations were kept at 40oC 1oC for 20days. The formulations were kept both at roomand elevated temperature and observed on 0th,5th, 10th, 15th and 20th day for the followingparameters [3, 4, 12,13].RESULTS AND DISCUSSIONRESULTS:pH of the Cream:The pH of the cream was found to be in the rangeof 5.6 to 6.8 which is good for skin pH. All theprepared formulations of cream are nearer to skinpH i.e. pH of F1-6.8 and F2-6.7.F16.8pHFormulationsF2F36.76.9Acidvalueand Saponification value:The results of acid and saponification value of allformulation of cream are presented in the table,and showed satisfactorily values.Table 4: Test for Acid & Saponification valueNOParametersFormulationF11.2.Acid value5.2Table 5: Irritancy effect of tionvalue22.326.228.2Irritancy test:The formulation shows no redness, edema,FormulationsF1F2F3NILLNILLNILLDye test:This dye confirms that all formulation was o/wtype emulsion cream. But formulation F1 showsmore stable in o/w type emulsion.Homogeneity:All prepared formulations produce uniformity ofcream.Homogeneitywasconfirmedbyappearance and by touch.Table 6: Homogeneity of oodGoodGoodAppearance:When formulation was kept for a long time, itfound that no change in the color of cream.Table 7: Appearance of formulationsParameterAppearanceTable 3: pH of the CreamParameterinflammation, and irritation during irritancystudies. These formulations are safe to use After feel:Emolliency, slipperiness and amount of residue leftafter the application of a fixed amount of creamwere found.Table 8: after feeling of formulationsParameterFormulationsF1F2F3After feelEmollientEmollientEmollientType of smear:After application of the cream, the type of smearformed on the skin was non-greasy.33 International Journal of Pharmacy Research & Technology Jan – Jun 2020 Vol 10 Issue 1
J.Saminathan et al / Formulation and evaluation of aloevera and dacus carota herbal creamTable 9: Type of smear of NongreasyNongreasyNongreasyTable 10: Removal of GoodGoodRemoval:The cream of F6 and F7 applied on the skin waseasily removed by washing with tap water.Accelerated stability studies:Table 11: Stability studiesNO1.Days0TemperatureRT400C 10C2.5RT400C 10C3.10RT400C 10C4.15RT400C 10C5.20RT400C F26.4**NCC**ENGESX1-Homogeneity, X2-Appearance, X3-Spreadability,X4-After feel, X5-Type of smear, X6-Removal,34 International Journal of Pharmacy Research & Technology Jan – Jun 2020 Vol 10 Issue 1
J.Saminathan et al / Formulation and evaluation of aloevera and dacus carota herbal cream**: Good,*: Satisfactory, E: Emollient, NG: Nongreasy, ES: Easy, NCC: No change in color.Discussion Aloe Vera and Daucus Carota are well knownfor its medicinal and Cosmeceuticals value inThe Indian traditional system of medicine.The present project work is to extract andprepare herbal cream. The tyrosinase inhibitory substances areused in cosmetic products as a skinwhitening agent to reduce skin pigmentationby decreasing the melanin production. The Aloesin, a C-glycosylated chromonereported to exhibit antityrosinase activity,and also inhibited melanin production in cellculture [22]. The aloesin present in the aloevera is an active compound. Aloe vera has been regarded as the mostpowerful and protective effect againstdamage to skin against the ultravioletradiation. due to the presence of itsantioxidant activity. Aloe vera contains mucopolysaccharides arebits of help in maintaining moisture into theskin. Aloe stimulates fibroblast which helpsin produces the collagen .that makes theskin more elastic and less wrinkled. Aloe’s benefits can be attributed at leastpartly to its nutrients since it ccharides), vitamins (includingB1, B2, B3, B6, C, and folic acid) andminerals[23]. The beneficial nutrients present in the aloeVera makes the skin smooth, moisturize,heal fastly and regenerates. [7, 14-17]. From above it concluded that these plantextracts produce excellent whitening, antiwrinkle and sunscreen effect on theskin[24]. production of free radicals causesoxidative stress and oxidative photodamageto membranes and molecules in the skin.The maturation of skin becomes wrinkledand rough. β -carotene is the most abundant and mostefficient precursor of vitamin A. β -carotene is called as a radicalscavenger[25]. Because it protects theskin from harmful UV radiations. β-carotene is capable to increase cellturn-over and regeneration in the outerlayers of the skin, making it effective fordiseases and skin conditions related toepithelium damage[26]. β-carotene also enhances the appearance ofdry or damaged skin by reducing flaking andrestoring suppleness[27]. In skincareproducts, beta-carotene is used to protect the skin from sun rays and also to protectsthe skin tone due to its anti oxidant andanti-aging properties [18, 19]. It is reportedthat Daucus Carota contains more amountsof vitamin C and an abundant amount of βcarotene.From the above discussion, it is assumedthat β-carotene containing plant as well asantioxidant activity producing plants can beused in face care cream, to producesunscreen, anti-aging, and anti-wrinkleeffects. Hence these two extracts are thebest choice to use in face creams.The almond oil has emollient propertiesand also gives smoothes, glow to theskin[28].The prepared formulation is a polyherbalface cream of O/W type emulsion, so thecream is hence can be easily washable withportable water.Therefore, we tried to make a polyherbalface cream containing the extract of AloeVera, and Daucus Carota.Our study indicated that the formulation F1found to be more stable while remainingformulations were not stable and resulted ina breakdown of the emulsion when storedfor a long time.This formulation had almost suitable andconstant pH, homogeneous, emollient, nongreasy and easily removed after theapplication. The stable formulation F1 wassafe with respect to skin irritation andallergic sensitization.ConclusionIt is concluded that on combining the extracts ofaloe Vera and Dacus Carota in differentcompositions to get multipurpose effect on skinsuch as whitening, anti-wrinkle, ant-aging, andsunscreen effect in skin. We know that it is notpossible to get efficiency effect with single herbbut by taking combinations of different extractscan be possible to increase the efficiency ofextracts. In this regard, we mixed the extracts ofaloe Vera and Dacus Carota to improve as wellsynergizes the cosmetic properties of preparedproducts compare to individual extracts. Thesestudies suggest that the composition of theextracts and base of F1 is more stable and safe.References1. Saraf, S. and Kaur, C.D., 2010. Phytoconstituentsas photoprotective novel cosmetic formulations.Pharmacognosy reviews, 4(7), p.1.2. Datta, H.S. and Paramesh, R., 2010. Trends inaging and skin care: Ayurvedic concepts. Journalof Ayurveda and integrative medicine, 1(2),p.110.35 International Journal of Pharmacy Research & Technology Jan – Jun 2020 Vol 10 Issue 1
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This dye confirms that all formulation was o/w type emulsion cream. But formulation F1 shows more stable in o/w type emulsion. Homogeneity: All prepared formulations produce uniformity of cream. Homogeneity was confirmed by appearance and by touch. Table 6: Homogeneity of formulations Parameter Formulations F1 F2 F3
Evaluation of Formulation. WHAT DO WE MEAN BY: ‘PSYCHOLOGICAL FORMULATION’? Some definitions: Formulation is a provisional explanation or hypothesis of how an individual comes to present with a certain disorder or circumstance at a particular-point in time. (Weerasekera, 1996) A formulation is a tool used by clinicians to relate theory to practice . It is the lynchpin that .
Formulation Development and Evaluation of Enteric Coated Tablets of Rabeprazole Sodium B.Rama*, Shalem Raju Talluri, Grace Rathnam Department of Pharmaceutics, C. L. Baid Metha College Of Pharmacy, Chennai Abstract: Rabeprazole sodium is highly acid-labile and presents many formulation challenges and to protect it from acidic environment of the stomach an enteric coated tablet formulation is .
Formulation and evaluation of almotriptan chewable tablets 1V. Anil kumar*, K.L. Deepthi*, 1R. Kalyani, 1B. Padmasri, 2D.Prasanth . in the formulation of a chewable tablet is to obtain a complete profile of the active drug. This usually leads to the most efficient formulation of a stable and quality product as the drug usually dictates the choice of fillers, carriers, sweeteners, flavor .
Ravindran et al. / Formulation and Evaluation IJPCR, Volume 8, Issue 9: September 2016 Page 1306 Tween 80 Figure 1: Fruits of Dimocarpus longan. Formulation 1 Formulation 2 Figure 2: Formulated antioxidant creams The added to a free radical Chemicals 2,2 -Diphenyl-1-picryl hydrazyl (DPPH) was obtained from Sigma Aldrich Co, St Louis, USA .
uniformity of films, surface pH, disintegration time and in-vitro dissolution studies. The formulation F5 has disintegration time of 56 seconds and is more promising and showed drug release of 99.89%; hence formulation F5 was selected as best formulation. Keywords: Oral Films, Metoprolol Succinate, Solvent Casting Method. INTRODUCTION
mixed. Since formulation containing antimicrobial agents as active moiety, it is likely to protect from microbial growth 8, 9. To determine the activity of formulation is subject to study the prepared formulation with standard method called standard cup plate method and the inhibition zone diameters were measured with the help of zone reader.
Citation: Arunadevi Birajdar., et al. "Formulation and Evaluation of Antimicrobial Hair Gel from Abrus Precatorius". Medicon Pharmaceutical Sciences 1.3 (2021): 02-13. Formulation and Evaluation of Antimicrobial Hair Gel from Abrus Precatorius 03 Figure 1: Alopecia. Many herbal products have been praised for their hair growth-promoting activities [10].
Division and 3-505 Parachute Infantry Regiment on 4 August 1990. My company, Charlie 3-505, had been conducting night live-fire exercises at Fort Bragg, North Carolina. Around 2230 hours on the night of 4 August, I received a Warning Order from my commander, Captain Charles Dydasco, to prepare for movement to the Battalion Area. Shortly after midnight, in a torrential downpour, we began .
