BioPak Point-of-use Ultrafilter - LabRepCo, LLC
BioPak point-of-use ultrafilterFinal purification step at the ultrapure water point-of-deliveryprovides pyrogen- and nuclease-free water at a high flow ratewhen you need it.The BioPak unit is a disposable ultrafiltrationcartridge typically used in cell culture, biochemistryor molecular biology applications. It can be installedat the outlet of Type I water purification systems suchas the Milli-Q , Direct-Q , Simplicity or Synergy systems, to produce pyrogen- and nuclease-freeultrapure water for a period of up to three months.The cartridge is composed of polysulfone hollow fibersin a white ABS housing. The BioPak ultrafiltrationmembrane is designed to optimize the rejection ofpyrogens, nucleases and bacteria, while maintaininga high flow rate and minimizing the release of ionicand organic materials.EMD Millipore is a division of Merck KGaA, Darmstadt, GermanyKey benefits Direct connection to all EMD Millipore Type Iwater systems Pyrogen-free water ( 0.001 EU / mL) production RNase-free water ( 0.01 ng / mL) andDNase-free water ( 4 pg / μL) production Safe method that eliminates the need totreat water with DEPC Bacteria-free water ( 1 cfu / mL) production Warranty of results within specifications fora minimum of 90 days usage Maintenance-free
Pyrogen removalThe most common pyrogens are endotoxins,i.e., lipopolysaccharides (LPS) from the walls ofGramnegative bacteria. The LPS have two major parts:a hydrophilic polysaccharide chain with antigenicregions and a hydrophobic lipid group. As thepolysaccharide chain is variable in length, the LPSmolecular weight ranges from 3,000 to 25,000 Dalton.In ultrapure water, the LPS sub-units aggregate toform higher molecular weight structures that can beremoved by ultrafiltration membranes with cut offbelow 20,000 Dalton.Pyrogens are known to affect cell culture andbiochemistry experiments in numerous ways. Ithas been demonstrated that their interaction withcell membranes causes morphological changesand damage, as well as the secretion of specificsubstances such as tumor necrosis factor, cytokines orenzymes. Pyrogens also affect the cell division process(enhancing or reducing it) depending on the nature ofthe cell line. The presence of pyrogens also may affectanalytical techniques such as electrophoresis.For these reasons, it is good laboratory practice toremove pyrogens from all solutions used in cell cultureand other biochemical applications. Experimentsperformed in EMD Millipore R&D laboratorieshave demonstrated that the BioPak ultrafiltrationcartridge can be used for at least 90 days to treatMilli-Q ultrapure water and obtain product waterwith a pyrogen level below 0.001 EU/mL.Nuclease removalChallenge tests performed in EMD Millipore R&Dlaboratories have demonstrated that the BioPak cartridge allows easy production of ultrapure waterthat is both RNase-free ( 0.01 ng/mL) and DNasefree ( 4 pg/μL).Previous experiments also have shown thatultrafiltration with a properly validated device is justas efficient as diethylpyrocarbonate (DEPC) for RNaseremoval from ultrapure water without the negativeaspects of DEPC treatment: lengthy treatment timeand contamination of the treated water by CO2 andethanol.2
Bacteria-free water productionHigh flow rateThe validation protocol provided proof that theBioPak ultrafiltration cartridge enables delivery ofbacteria-free ( 1 cfu/mL) water, when used accordingto instructions with the filter outlet located in a cleanenvironment.The large ultrafiltration membrane surface of theBioPak cartridge makes it possible to producepyrogen-free ultrapure water without compromisingthe flow rate, as shown by the graph opposite.BioPak ultra filtrationcartridge flow rateThe user can obtain ultrapure water on demandjust before solution preparation, minimizing risks ofrecontamination.Certificate of QualityEach BioPak unit has been individually testedfor efficiency and flow rate and is delivered with aCertificate of Quality.Feed pressure 1 barFlow rate (l/min)2.5ORDERING logue No.BioPak Ultrafiltration Cartridge (1/pk),validated for pyrogen- and nucleasefreewater production, delivered with aself-adhesive label (with space to noteinstallation and replacement dates),Certificate of Quality and multilingualUser manual.CDUFBI001Water temperature ( C)Product water specificationsPyrogen Level (EU/mL) 0.001RNases* (ng/mL) 0.01DNases* (pg/μL) 4Bacteria* (cfu/mL) 1* When used according to instructions with the filter outlet locatedin a clean environment3
Lit. No. PB1006ENUS 01/12. Copyright 2012, EMD Millipore, Billerica, MA, U.S.A.Millipore, Milli-Q, Direct-Q, Simplicity, BioPak and Synergy are registered trademarksof Merck KGaA, Darmstadt, Germany. The M Mark and EMD Millipore are trademarksof Merck KGaA. All rights reserved. Design: Sysaxe4
LC-Pak Point-of-use PolisherC18 Reverse phase silicaKey Benefits The LC-Pak cartridge is easily connected to theoutlet of all EMD Millipore Type I water systems (Milli-Q , Direct-Q , Synergy and Simplicity ) Final purification step uses efficient C18 reversephase silica technology to provide fresh ultrapurewater with low traces of organics-at high flow rate,when you need it Delivers a minimum of 500 L of organic-free ultrapure water Designed to answer the demands of UPLC, LC-MSand LC-MS/MS analytical techniques used for organictrace and ultra-trace analysis Validated for the production of water whose qualityequals or exceeds the specifications of bottledwater for LC-MS Each LC-Pak cartridge is delivered with a Certificateof QualityRecent advances in analytical techniques such as UPLC,LC-MS, LC-MS / MS have considerably improved thesensitivity of organic and biochemical molecule detection.As a result, these techniques now require improvedwater quality for the production of mobile phase,buffers, blanks, standards preparation, sample dilution,glassware rinsing or extraction.EMD Millipore is a division of Merck KGaA, Darmstadt, Germany
Although fresh Milli-Q water has proved adequatefor most procedures involving these techniques untilrecently, there is now a growing need for higher waterquality in some advanced applications.To answer this emerging need, EMD Millipore hasdeveloped a new POD-Pak designed to produceultrapure water with an organics contaminationlevel below most water sources available today, at afraction of the cost.The LC-Pak cartridge can be connected to the outletof existing and new EMD Millipore Type I water systems(Milli-Q , Direct-Q , Synergy and Simplicity ) toproduce water with a TOC level below 5 ppb and deliverat least 500 L of ultrapure water with minimum traceorganic contamination. The LC-Pak cartridge uses thewell-known reversed-phase silica purification media toremove traces of neutral organics.LC-PAK CARTRIDGE DESIGNThe LC-Pak is designed to provide scientists with the certitude that the ultrapure water that they are using incritical organic molecules analysis at trace and ultra-trace levels will be adequate.Polypropylene CompressionDisc (Sintered Filter)and Luer FC18 reversephase silica(25 gram)Polypropylene VyonPolypropylene Housingand Luer FHose connection foreasy water deliveryThe granular C18 reverse phase silica in the polypropylene housing is tightly packed by a compression disc toavoid any channeling effect. This ensures that the water passing through the LC-Pak will be in close contactwith the C18 chains binding organic molecules by hydrophobic interactions.When required, the LC-Pak outlet can be connected to a 0.22 μm in-line filter.2
LC-PAK CARTRIDGE OPERATIONThe LC-Pak is easy to use: simply flush it with 50 mL methanol to properly wet the pores of the C18 reversephase purification media, connect the LC-Pak to the outlet of the EMD Millipore ultrapure water system andflush with ultrapure water for 10 minutes. You are now ready to deliver at least 500 L of ultrapure water withlow trace organic.LC-PAK CARTRIDGE SPECIFICATIONSThe LC-Pak cartridge was extensively validated to ensure that the water quality at the outlet matches themost demanding requirements.ParameterHPLC Gradient Test - Absorbance ofhighest eluted peakHPLC Gradient Test - Absorbance ofhighest eluted peakOptical properties:absorbance in UV rangeFluorescence as quinineCompliance with suitability for LC/MS:Reserpine testResidue after evaporationSpecification of LC-Pak ultrapure waterCommentsZRXQ003 WW*Concentration of 60 mL waterat 1 mL/min prior to elutionAt 210 nm 0.006 AUAt 254 nm 0.002 AUUV 200 nm 0.05 AUUV 205 nm 0.01 AUUV 210 nm 0.01 AUUV 254 nm 0.005 AUAt 254 nm 1 ppbAt 365 nm 1 ppbNo peak higher than10 ppbReserpine at 609 m/z inESI 0.0001 % w/wNo water preconcentrationTest performed as specifiedin ISO 3696 procedureAn LC-Pak validation guide is available should you wish to review the details of the validation report.OPTIONALMillipak 0.22 μm end filterMPGP040013
COMPARISON OF LC-PAK AND SOME LC-MS BOTTLED WATERIn order to verify that fresh ultrapure water produced by a Milli-Q system and an LC-Pak cartridge matchedor exceeded the quality of commercially available bottled water for LC-MS applications, different 60 mL watersamples were pre-concentrated on a C18 reverse phase chromatography column, then eluted and an lyzed byLC-MS (PDA, single quadrupole). Examples of the results obtained are shown below.Bottled water F for LC-MSBottled water J for LC-MSBottled water R for LC-MSBottled water B for LC-MSMilli-Q Integral with LC-Pak EQUIPMENTThe equipment used throughout the experiment was from Waters Corporation, Milford, MA, U.S.A. LC system: Alliance 2695 Pre-column Column: C18 X-Terra ; 3 μm, 4.6 x 30 mm Column: C18 Atlantis; 3 μm, 2.1 x 150 mm Detectors: PDA Model 2996 Mass detector ZQ 2000 Elution solvents: Water to be tested, Acetonitrile HPLC grade (J.T. Baker) Computer for data acquisition and system control equipped with Empower softwareDetailed analytical conditions available upon request: internal procedure No. 00084229SO.The entire study, including details of analytical procedures, is provided in the LC-Pak cartridge Validation Guide.ORDERING INFORMATIONDescriptionLC-Pak Cartridge (1/pk) delivered hermetically sealed,with a Certificate of QualityCartridge installation and Conditioning Kit including the followingreusable parts: Polyethylene ¼ Gaz F-Hose barb connection with O-ring Polyethylene ¼ G F – ¼ Gaz F connectors for connection to Millipak CommentsMillipak 0.22 μm end filterMPGP04001Millipore, Milli-Q, Direct-Q, Synergy, LC-Pak, Simplicity and Millipak are registered trademarks of Merck KGaA, Darmstadt, Germany.The M mark, EMD Millipore are trademarks of Merck KGaA. Waters, Alliance, XTerra, Atlantis and Empower are registered trademarks ofWaters Investment Ltd., and ZQ is a trademark of Waters Investment Ltd.Lit. No. DS1067ENUS 2012 EMD Millipore, Billerica, MA. U.S.A. All rights reserved.4LCPAK0001EDSKIT001
EDS-Pak Point-of-Use PolisherEndocrine disrupter-free water at the ultrapure waterpoint of deliveryKey benefits:Endocrine disrupters Point-of-use polisher for use with Milli-Q and other EMD Millipore Type I waterpurification systemsEndocrine disrupters are small, man-madeorganic chemicals whose structures mimicthose of hormones, enabling them to interactwith normal endocrine system functions.By sheer coincidence, their key-shapedmolecules open the hormonal locks thatcontrol proper development and behavior inboth humans and animals.1,2,3 Final purification step provides endocrinedisrupter-free ultrapure water at high flowrate—when you need it Delivers a minimum of 300 l of EDS-freeultrapure water Validated for efficient removal of bisphenolA, diethyl phthalate and di-n-butyl phthalate Each EDS-Pak cartridge is delivered witha Certificate of QualityEMD Millipore is a division of Merck KGaA, Darmstadt, Germany
Today, endocrine disrupters are increasinglypresent in the environment. Althoughendocrine disrupters may be only somewhatdangerous for adults, mounting evidencesuggests that they can significantly disturbfetal and infant development. As researchersincreasingly focus on the effects of thesechemicals, there is a need for endocrinedisrupter free water for use in experimentaltests. While conventional water purificationtechniques remove the bulk of organiccompounds from ultrapure water, some traceorganics still may remain.These man-made chemicals can be foundin many products widely used in industrialsocieties: Bisphenol A is a plasticizer used in reusablewater bottles, laptop computer housings,dental sealants and the resins in some foodcan linings. Phthalates are found in a wide varietyof products, including vinyl flooring, foodpackaging, blood-storage bags, intravenousmedical tubing, as well as many health andbeauty products such as detergents, soaps,shampoos, deodorants, fragrances, hair sprayand nail polish. They are often used as aplasticizer in PVC pipes distributing water inbuildings.Examples of endocrine disrupters includenonylphenol (a detergent), bisphenol A (aplasticizer), diethylstilbestrol (a growthagent administered to livestock) and DDT (apesticide).Examples of man-made chemicalswith estrogeno-mimetic hylstilbestrolCIBisphenol AHOCH3CH3DDTCIH2CCI3
EDS-Pak polisher designEDS-Pak polisher operationThe EDS-Pak is a disposable point-of-useultrafiltration cartridge developed by EMDMillipore to meet the needs of researcherswho require ultrapure water free of endocrinedisrupters (EDS).The EDS-Pak cartridge contains a specifictype of activated carbon that has beenvalidated for the removal of several endocrinedisrupters. The cartridge’s housing andconnector materials also have been validatedfor the absence of endocrine disrupters.The EDS-Pak can be installed on allEMD Millipore Type I water purificationsystems that deliver ultrapure water withTOC 5 ppb, including the Milli-Q ,Synergy , Simplicity and Direct-Q systems. It is designed to provide at least300 l of EDS-free ultrapure water beforereplacement is necessary.Before use, the EDS-Pak unit must beconditioned with methanol to ensure that allbinding surfaces are accessible to endocrinedisrupters.*Following this step, the cartridge should thenbe flushed with ultrapure water to removeany excess methanol. Once conditioned, theEDS-Pak unit is warranted to deliver up to300 l of EDSfree water (see specifi cationsbelow) when fed by ultrapure water with aTOC level 5 ppb at a fl ow rate between0.5 – 2 l/min.Inlet Connection ¼"Male and Luer FOutlet Connection ¼″Male and Luer FActivated Carbon(50 ml)Polyethylene HousingHose Connection(with O-ring) for easyLength 12.7 cmEDS-Pak polisher specificationsThe EDS-Pak has been validated for the effi cient removal of the following endocrine disrupters: bisphenol A,diethyl phthalate, di-n-butyl phthalate and nonylphenol.EDS testedBisphenol ADiethyl phthalateFeed (ppb)Up to 4Up to 1.5Product (ppt) DL (5 ppt) DL (200 ppt)Volume (l)300300Flow (l/min)0.5 – 2.00.5 – 2.0Di-n-butyl phthalateUp to 1.5 DL (200 ppt)3000.5 – 2.0NonylphenolUp to 3.3 DL (100 ppt)3000.5 – 2.0Each EDS-Pak cartridge is delivered with a Certificate of Quality showing the analytical results of theEndocrine Disrupter Analysis performed on the cartridge lot.3
Ordering informationDescriptionEDS-Pak Cartridge (1/pk) delivered hermetically sealed, witha Certificate of QualityCatalogue No.EDSPAK001EDS-Pak Cartridge installation and Conditioning Kitincluding the following reusable parts: Polyethylene ¼ Gaz F-Hose barb connection with O-ring EDSKIT001 Polyethylene ¼ G F – ¼ Gaz F connectors for connectionto Millipak *Note: A 50 ml glass syringe is required to condition the EDS-Pak cartridge with methanol. Thissyringe is not provided by EMD Millipore.Bibliography1. Huang, Y.W.; Twidwell, D. L.; Elrod, J.C. “Occurrence and Effects of Endocrine Disrupting Chemicalsin the Environment,” Practice Periodical of Hazardous, Toxic, and Radioactive Waste Management, 7(4), p. 241-247 (2003).2. Brevini, T.A.L.; Zanetto, S.B.; Cillo, F. “Effects of Endocrine Disrupters on Developmental andReproductive Functions,” Current Drug Targets – Immune, Endocrine & Metabolic Disorders, Volume 5,Number 1, p. 1-10 (2005).3. Bigsby, R.; Chapin, R. E.; Daston, G. P.; Davis, B.J.; Gorski, J.; Gray, L.E.; Howdeshell, K.L.; Zoeller, R.T. and vom Saal, F. S. “Evaluating the Effects of Endocrine Disrupters on Endocrine Function duringDevelopment,” Environmental Health Perspectives 107 (Suppl. 4), p. 613-618 (1999).4Lit No. PB1234ENUS01/12 Copyright 2012 EMD Millipore, Billerica, MA, U.S.A., Milli-Q, EDS-Pak, Synergy, Simplicity, Direct-Q and Millipakare registered trademarks of Merck KGaA, Darmstadt, Germany. The M mark, EMD Millipore are trademarks of Merck KGaA.All rights reserved
VOC-Pak Point-of-Use PolisherFinal purification step at the ultrapure waterpoint-of-delivery efficiently removes VolatileOrganic Compounds (VOCs)VOCs are industrial contaminants that may causehealth safety issues and therefore should be detectedin the environment. VOCs are routinely analyzed inwater, soil, soil gas and air.Specifications for VOCs in drinking water have beenestablished by different regulatory agencies in manycountries. A table showing the Maximum AllowableConcentration (MAC) for some of the different VOCsmonitored in the United States, Europe, Japan andChina, as well as by the World Health Organization(WHO), is available on the EMD Millipore web site:www.millipore.com/labwaterBenefits The VOC-Pak polisher is easily connected to theoutlet of all EMD Millipore Type I water purificationsystems (Milli-Q , Direct-Q , Synergy andSimplicity systems) The final purification step provides VOC-freeultrapure water at high flow rate—when you need it Delivers a minimum of 300 l of VOC-free ultrapurewater Validated for efficient removal of VOCs Each VOC-Pak cartridge is delivered with aCertificate of QualityEMD Millipore is a division of Merck KGaA, Darmstadt, Germany
VOC-Pak POLISHER DESIGNThe VOC-Pak cartridge is designed to assurescientists performing VOC analysis that the ultrapurewater they use to rinse glassware, produce standardsolutions or dilute samples is VOC-free.Polypropylene Sintered FilterInlet Connection ¼Male and Luer FOutlet Connection ¼Male and Luer FActivated Carbon(50 ml)Polyethylene HousingLength 12.7 cmHose Connection (withO-ring) for easy waterdeliveryThe purification media used to achieve this goalis a specific activated carbon selected after testsperformed on media from many suppliers.Table 1:Results of the VOC-Pak challenge test for a processedvolume of 350 l2VOC testedQuantificaChallengetion limit(ppb) (*)(ppb) (**)Concenctration afterVOC-Pak (ppb) (***)benzene0.97 0.05 0.05bromodichloromethane1.56 0.05 0.05bromoform1.58 0.50) 0.50carbon tetrachloride1.06 0.05 0.05chlorobenzene0.94 0.05 0.05chloroform1.07 0.05 0.05dibromochloromethane1.01 0.10 0.101,2-dichlorobenzene1.01 0.05 0.051,4-dichlorobenzene1.53 0.05 0.051,2-dichloroethane1.08 0.05 0.051,1-dichloroethene0.87 0.05 0.05cis-1,2-dichloroethene0.95 0.05 0.05trans-1,2-dichloroethene1.53 0.05 0.051,2-dichloropropane0.86 0.05 0.05cis-1,3-dichloropropene0.97 0.04 0.04trans-1,3-dichloropropene0.91 0.05 0.05ethylbenzene1.09 0.05 0.05tetrachloroethene1.44 0.05 0.05toluene0.96 0.10 0.101,1,1-trichloroethane0.94 0.10 0.101,1,2-trichloroethane0.93 0.05 0.05trichloroethene1.02 0.05 0.05o-xylene0.79 0.05 0.05m-xylene1.02 0.05 0.05p-xylene0.94 0.05 0.05(*) Challenge VOC concentration in feed water upstream ofVOC-Pak cartridge(**) Quantification limit: limit of quantification of the VOCmeasured by GC(***) Concentration of VOC below the VOC-Pak cartridge for afeed water concentration of 1 ppbVOC-Pak POLISHER OPERATIONThe VOC-Pak cartridge is easy to use: simply connectthe VOC-Pak unit to the outlet of the EMD Milliporeultrapure water system and flush 5 l of water. Youare now ready to deliver at least 300 l of VOC-freeultrapure water.VOC-Pak POLISHER SPECIFICATIONSThe VOC-Pak cartridge is designed to producewaterin which the VOC concentration is below theanalytical quantification limit (**) for the VOCs listedin Table 1, provided that the VOC concentration infeed water does not exceed 1 ppb (1 μg/l), and theTotal Oxidizable Carbon (TOC) of the ultrapure waterentering the VOC-Pak cartridge is below 5 ppb.In order to warrant this water quality, theVOC-Pak cartridge was qualified by a challenge test:the VOC-Pak cartridge was fed by ultrapure watercontaining a 1 ppb (1 μg/l) concentration of the VOCto be eliminated.The concentration of the VOC was measured by GasChromatography (GC) downstream of the VOC-Pak cartridge after passage of 50, 100, 150, 200, 250,300 and 350 l in order to determine the breakthroughpoint of the different VOCs. The results are displayedin Table 1 for a processed volume of 350 l.Q-POD dispenser fitted with a VOC-Pak cartridge
Analysis of the VOCs in ultrapure water was performedaccording to EMD Millipore procedure 84214SO,using an Eclipse 4660 Purge-and-Trap sampleconcentrator from OI CorporationTM (College Station,Texas) and a GC-MS system (GC model 6890N and MSmodel 5975B) from Agilent Technologies , Inc., SantaClara, California. A copy of the complete qualificationreport with description of the analytical procedure isavailable upon request.Additionally, each lot of VOC-Pak cartridges isanalyzed to ensure that the following VOCs (Table 2)are below the analytical quantification limit in waterproduced by a VOC-Pak fed with ultrapure waterfrom a EMD Millipore Milli-Q system (resistivity at18.2 MΩ.cm at 25 C and TOC 5 ppb).Table 2:Quantification limits (ppb) of VOCs analyzed in eachlot of VOC-Pak cartridgesCompoundQL* (ppb)CompoundQL* (ppb)benzenebromobenzene 0.05 0.102,2-dichloropropane 0.051,1-dichloropropene 0.05bromochloromethane ylbenzenesec-butylbenzenetert-butylbenzenecarbon 2-dibromo-3chloropropane chloropropane1,3-dichloropropane 0.05 0.50 0.20 0.05 0.50 0.05 0.05 0.05 1.00 0.05 0.05 0.10 -isopropyltoluenemethylene nyl chloride 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.10 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.10 0.10 0.10 0.50 0.10 0.05 0.10 0.05 0.10 0.05 0.05 0.10 0.05 0.05 0.10 0.05 0.05 0.10 0.05 0.05 0.05 0.20*QL Quantification Limit – expressed in ppb (μg/l)CCI4C6H5CICHCI3C2H3CIMolecules removed by the VOC-Pak cartridgeinclude carbon tetrachloride (CCl4) andchlorobenzene (C6H5Cl).Molecules removed by the VOC-Pak cartridgeinclude chloroform (CHCl3) and vinyl chloride(C2H3Cl).Milli-Q water purification system fitted with aVOC-Pak cartridgeDirect-Q 3 water purification system withVOC-Pak cartridge3
Ordering informationDescriptionVOC-Pak cartridge (1/pk) delivered hermetically sealed, with a Certificate of QualityCartridge installation and Conditioning Kit including the following reusable parts: Polyethylene 1/4 Gaz F-Hose barb connection with O-ring Polyethylene 1/4 Gaz F – 1/4 Gaz F connectors for connection to Millipak final filterFor more information, please visit our website:www.millipore.com/labwater4Millipore, Milli-Q, Direct-Q, Synergy, Simplicity, and Millipak are registered trademarks of Merck KGaA, Darmstadt, Germany. VOC-Pak,the M mark and EMD Millipore are trademarks of Merck KGaA. OI Corporation is a trademark of OI Corporation. Agilent Technologies isa registered trademark of Agilent Technologies, Inc.Lit. No. PB1632ENUS 01/12 2012 EMD Millipore, Billerica, MA U.S.A. All rights reserved.Catalogue No.VOCPAK001EDSKIT001
(Milli-Q , Direct-Q , Synergy and Simplicity ) to produce water with a TOC level below 5 ppb and deliver at least 500 L of ultrapure water with minimum trace organic contamination. The LC-Pak cartridge uses the well-known reversed-phase silica purification media to remove traces of neutral organics. LC-PAK CARTRIDGE DESIGN
NIOSH Approval Label 4 I. Cautions and Limitations 5 II. S. Special or Critical User’s Instructions 5 III. Theory of Biopak Operation 7 A. Biopak Description 7 B. Breathing Loop 9 C.
AirCel. The price of ineffective and inefficient filtration Liquid water entering refrigerated air dryer. Inefficient dryer operation leading to higher cost and/or inability to meet dew point. Damage to dryer components such as heat exchanger. Inst
One Point Perspective: City Drawing A Tutorial Engineering 1 Tatum. When completing this tutorial, you must use the following items: * White, unlined paper * A ruler or other straight-edge * A pencil. Begin by setting up your paper for a one-point perspective drawing. Draw a horizon line and a vanishing point. Draw two orthogonals (diagonal .File Size: 727KBPage Count: 41Explore furtherOne point perspective city: The step by step guide .pencildrawingschool.comHow to Draw One Point Perspective City Printable Drawing .www.drawingtutorials101.comOne Point Perspective Drawing Worksheets - Learny Kidslearnykids.comPerspective Drawing - An Easy Lesson in 1 Point .www.drawinghowtodraw.comThe Helpful Art Teacher: Draw a one point perspective city .thehelpfulartteacher.blogspot.comRecommended to you b
Tests to ensure all carbonaceous contamination is removed include: Measuring the quantity of carbon remaining on the ultrafilters after they have been cleaned. To achieve this, an ultrafilter is selected (randomly) for carbon content analysis. No measurable carbon was found, indicating that the ultrafilters had been cleaned satisfactorily.
Applications - LHC collimation region Regions of high losses (e.g., Collimators, « ) ATLAS Regions with low losses (e.g., due to residual gas) The LHC Loss Regions Point 1 Point 2 Point 3.2 Point 3.3 Point 4 Point 5 Point 6 Point 7 Point 8 ALICE LHCb Momentun Cleaning RF CMS LHC Dump Betatron Cleaning FLUKA geometry visualized with SimpleGeo .
Applications -LHC collimation region Regions of high losses (e.g., Collimators,« ) ATLAS Regions with low losses (e.g., due to residual gas) The LHC Loss Regions Point 1 Point 2 Point 3.2 Point 3.3 Point 4 Point 5 Point 6 Point 7 Point 8 ALICE LHCb Momentun Cleaning RF CMS LHC Dump Betatron Cleaning FLUKA geometry visualized with flair .
Applications -LHC collimation region Regions of high losses (e.g. , Collimators, « ) ATLAS Regions with low losses (e.g. , due to residual gas) The LHC Loss Regions Point 1 Point 2 Point 3.2 Point 3.3 Point 4 Point 5 Point 6 Point 7 Point 8 ALICE LHCb Momentun Cleaning RF CMS LHC Dump Betatron Cleaning FLUKA geometry visualized with .
An industry code of practice is approved by the Minister for Commerce. It takes effect on the day specified in the code or, if no day is specified, on the day it is published in the NSW Government Gazette. An approved industry code of practice may be amended from time to time (or it may be revoked) by publication in the gazette. An approved industry code of practice is designed to be used in .
