Diagnostic Rapid Test - Zoetis US

DIAGNOSTICRAPID TESTS1

With diagnostic rapid tests from Zoetis, you get more of what you want andless of what you don’t want: more options, more confidence, less hassle,less waiting. VETSCAN and WITNESS diagnostic rapid tests offer you theflexibility to do single or multiplex testing, so you can test for what is neededto make the right clinical decisions, and prevent wasted time and expense.Get accurate, fast, and clear point-of-care results with Zoetis.Zoetis investigates all reported discrepant or unexpected results.2VETSCAN Technical Support24/71 800 822 2947WITNESS Technical SupportMonday-Friday 9am-7pm ET1 888 ZOETIS1 (963-8471)Confirmation testing available for:VETSCAN Flex4VETSCAN HeartwormVETSCAN LymeVETSCAN AnaplasmaVETSCAN EhrlichiaConfirmation testing available for:WITNESS HeartwormWITNESS FeLV-FIVWITNESS FFH

Canine Infectious Disease4VETSCAN Flex45VETSCAN Heartworm6WITNESS Heartworm6WITNESS Lepto7Tick-Borne Disease8VETSCAN Anaplasma9VETSCAN Lyme9VETSCAN Ehrlichia9Feline Infectious Disease10VETSCAN FeLV/FIV11WITNESS FeLV/FIV11WITNESS FeLV12WITNESS FFH13Fecal14VETSCAN Parvo15WITNESS Parvo15VETSCAN Giardia16PancreaticVETSCAN cPLReproduction171819WITNESS LH20WITNESS Relaxin21Lateral Flow Technology22Lateral Flow Diagram23Also available for tick-borne disease testingAlso available for feline testingMust be read with the VETSCAN VUE Rapid Test Analyzer3

CANINE INFECTIOUSDISEASE4

FLEX4 Rapid TestLeahThe VETSCAN Canine Flex4 Rapid Test is a convenient and easyto-use visual rapid test for the qualitative detection of antibodies toAnaplasma phagocytophilum, A. platys, Borrelia burgdorferi, Ehrlichiacanis, E. chaffeensis, E. ewingii, and Dirofilaria immitis antigens incanine whole blood, serum or plasma.Also available for tick-borne disease testing25Read at8 minutesRoom temperaturestorageAvailable in25 test kitTest InstructionsCLACLAFLEX41CHEAdd 1 drop of anticoagulatedwhole blood, serum or plasma toeach sample well.FLEX42CLACHEAdd 3 drops of the chase bufferprovided to each sample well.FLEX43CHERead results at 8 minutes.Heartworm/Ehrlichia PositiveExample5

Heartworm Rapid TestVETSCAN and WITNESS Heartworm Rapid Tests are convenient,easy-to-use visual rapid tests for the qualitative detection of Dirofilariaimmitis antigen associated with heartworm disease in dogs and cats.Also available for feline infectious disease testingHeartwormVETSCANTest Instructions1Heartworm2Add 1 drop ofanticoagulated wholeblood, serum, or plasmato the sample well.Add 2 drops of chasebuffer provided to thesample well.HeartwormRead results at 10minutes. PositiveExample3Read at10 minutesRoom temperaturestorage25100VETSCAN available in25 test kit & 100 test kitHWWITNESSTest Instructions6HWHW101Add 1 drop ofanticoagulated wholeblood, serum, or plasmato the sample well.2Add 2 drops of chasebuffer provided to thesample well.3Read results at 10minutes. PositiveExample25WITNESS available in10 test kit & 25 test kit

Lepto Rapid TestWITNESS Lepto detects the primary immune response(immunoglobulin M [IgM] antibodies) that develops withinone week of exposure to canine leptospires.1,25Read at10 minutesRoom temperaturestorageAvailable in5 test kitTest InstructionsLEP1Grip the pipette by the stem. Hold a tube of sample and pipetteat an angle to allow proper filling of pipette. Without squeezingthe bulb, place the tip of the pipette in the sample. The capillarytube in the pipette will fill automatically in 2-3 seconds.2Touch the tip of the pipette to thesample pad and carefully squeezethe bulb to express the sample (5 µl),making sure fingers are covering thevent holes on the pipette bulb.LEPLEP3Add 3 drops of chase buffer provided tothe sample well. Read results at 10 minutes.Positive ExampleRemove pipette from sample when the capillary tube has filled.7

TICK-BORNEDISEASE8

Anaplasma Rapid TestThe VETSCAN Canine Anaplasma Rapid Test is a convenient and easy-to-usevisual rapid test for the qualitative detection of antibodies to A. phagocytophilumand/or A. platys in canine whole blood, serum, or plasma.Read within8-10 minutesLyme Rapid TestThe VETSCAN Canine Lyme Rapid Test is a convenient and easy-to-usevisual rapid test for the qualitative detection of antibodies to Borreliaburgdorferi in canine whole blood, serum, or plasma.Room temperaturestorage25Ehrlichia Rapid TestAvailable in25 test kitThe VETSCAN Canine Ehrlichia Rapid Test is a convenient and easy-to-usevisual rapid test for the qualitative detection of antibodies to E. canis, E.chaffeensis, and E. ewingii in canine whole blood, serum, or plasma.Test InstructionsAnaplasma1Add 1 drop of anticoagulatedwhole blood, serum, or plasma tothe sample well.Lyme2Add 3 drops of chase bufferprovided to the sample well.Ehrlichia3Read results within 8-10 minutes.Positive Example9

FELINE INFECTIOUSDISEASE10

FeLV/FIV Rapid TestVETSCAN and WITNESS FeLV/FIV Rapid Tests are convenient, easy-to-use, in-clinic test kitsfor the qualitative detection of feline leukemia virus antigen and feline immunodeficiencyvirus antibodies in feline whole blood, serum, or plasma.VETSCAN now availableas a single strip testFeLVVETSCANTest Instructions1FIVC L IFeLV2Add 1 drop ofanticoagulated wholeblood, serum, or plasmato the sample well.FIVC L IAdd 2 drops of chasebuffer provided to thesample well.FeLVFIVC L IRead results at 10minutes. FIV PositiveExample3Read at10 minutesRoom temperaturestorageWITNESSTest InstructionsFIVFIVFIVFeLVFeLVFeLV1Add 1 drop ofanticoagulated wholeblood, serum, or plasmato each sample well.2Add 2 drops of chasebuffer provided to eachsample well.3Read results at 10minutes. FIV PositiveExample1025Available in 10 test kit& 25 test kit11

FeLV Rapid TestWITNESS FeLV is a convenient, easy-to-use, in-clinic test that detects thepresence of the feline leukemia virus antigen that is found in high levels ininfected cats. Antigen test results are not affected by FeLV vaccination ormaternal antibodies. Kittens can be tested at any age.10Read at10 minutesRoom temperaturestorageAvailable in10 test kitTest InstructionsFeLV112Add 1 drop of anticoagulatedwhole blood, serum, or plasma tothe sample well.FeLV2Add 2 drops of chase bufferprovided to the sample well.FeLV3Read results at 10 minutes.Positive Example

FFH Rapid TestWITNESS FFH is a triple test kit that detects antigens to felineheartworm and feline leukemia (FeLV) and antibodies to felineimmunodeficiency virus (FIV).10Read at10 minutesRoom temperaturestorageAvailable in10 test kitTest Instructions1Add 1 drop of anticoagulatedwhole blood, serum, or plasma toeach sample well.2Add 2 drops of the chase bufferprovided to each sample well.3Read results at 10 minutes.FeLV Positive Example13

FECAL14

Parvo Rapid TestVETSCAN and WITNESS Parvo Rapid Tests are convenient and easy-touse in-clinic tests for the qualitative detection of parvovirus antigens incanine feces.3Read VETSCANwithin 10-15 minutesParvoVETSCANTest Instructions1Coat the swab uniformly with athin layer of sample.Break theseal to release sample diluent.Squeeze the bulb 5 to 6 times tocompletely mix the sample.2ParvoTransfer 3 dropsof sample into thesample well.Read results within10-15 minutes. PositiveExample3Read WITNESSat 10 minutesRoom temperaturestorage2010VETSCAN available in10 test kit & 20 test kitCPVWITNESSTest InstructionsCPV51Dispense buffer into tube up tograduation mark. Collect fecesswab and insert it into the tube.Tap to mix. Break the top of theswab at the blue line.2Invert and use thedevice to dispense 5drops of sample.3Read results at 10minutes. PositiveExampleWITNESS available in5 test kit15

Giardia Rapid TestThe VETSCAN Canine Giardia Rapid Test is a highly sensitive and specific test forthe qualitative detection of Giardia cyst antigens in canine feces.Read within10-15 minutesRoom temperaturestorageGiardiaTest Instructions1Coat the swab uniformly with athin layer of sample.Break theseal to release sample diluent.Squeeze the bulb 5 to 6 times tocompletely mix the sample.2Transfer 3 dropsof sample into thesample well.Giardia310Read results within10-15 minutes. PositiveExample20Available in 10 test kit& 20 test kitFecal Sample Collection Guide for Parvo and Giardia Rapid TestsThe sample amountis insufficient and notrecommended for testing.16The swab is coated uniformly with a thinlayer of sample. It is recommended to usethis amount of fecal sample for testing.The sample amount is excessiveand not recommended fortesting.

PANCREATIC17

cPL Rapid TestThe VETSCAN Canine Pancreatic Lipase (cPL) Rapid Test is aconvenient and easy-to-use in-clinic test for the semi-quantitativedetection of pancreas-specific lipase in canine serum or plasma.Must be read with the VETSCAN VUE Rapid Test Analyzer10Must be read withthe VETSCAN VUERoom temperaturestorageAvailable in10 test kitTest InstructionsRead with1Add 1 drop of serum or plasma* tothe sample well.*Serum or plasma only - no whole blood18Read withcPL2cPLAdd 3 drops of C hase Buffer to thesample well.Read with3cPLInsert cassette into VUE. Initiate aTimed Scan in the VUE app.Check mobile device display fornumeric result when timer completes.

REPRODUCTION19

LH Rapid TestWITNESS LH helps pinpoint the optimum time for canine breeding byidentifying the day of the luteinizing hormone (LH) surge. It may alsoaid in distinguishing between ovariectomized female dogs or catsfrom those that are sexually intact.Also available for feline reproduction testing6Read at20 minutesRoom temperaturestorageAvailable in6 test kitTest InstructionsLH1Add 4 drops of serum* to thesample well.*Serum only - no whole blood or plasma20LH2Read results at 20 minutes.LH Positive Example*Test Line “2” is darker or similar in intensity toControl Line “3”

Relaxin Rapid TestWITNESS Relaxin offers an early, reliable means for determiningpregnancy status in dogs and cats.Also available for feline reproduction testing5Read at10 minutesRoom temperaturestorageAvailable in5 test kitTest InstructionsRLX1Add 2 drops of serum or plasma*to the sample well.RLX2Add 2 drops of the chase bufferprovided to the sample well.RLX3Read results at 10 minutes.Positive Example*Serum or plasma only - no whole blood21

LATERAL FLOWTECHNOLOGY22

Highly accurate results, delivered through proven lateral flow technologyLateral flow technology has served as a reliable tool for efficient diagnosis and patient evaluation inveterinary and human medicine for more than 30 years.Antibody Lateral FlowAntigen Lateral FlowMagnification ofthe test “T” lineMagnification ofthe test “T” lineThe conjugate pad is pre-treated with specific colloidalgold-conjugated peptides and colloidal gold-conjugatedsignal amplification proteins. The membrane is precoated with antibody specific peptides that have beenlinked to a protein carrier at the Test “T” line.CCSample and specific colloidal gold-conjugatedantibodies combine in the conjugate pad creatingantigen-antibody-gold conjugate. The test ispre-coated with antigen-specific antibodies at thetest “T” line.TTWhen the sample flows through the conjugate padboth colloidal gold conjugates bind to any specificantibodies present in the sample.When the gold labeled antibodies reach the test “T”line specific antibodies bind to the test “T” line formingan “amplified sandwich” complex which has beenaugmented by Abaxis’ patented signal amplificationtechnology. The accumulation of the amplified sandwich complexes creates a visible signal.The conjugates flow across the membrane andbind to the antibodies in the sample line forming a“sandwich”.CTThe accumulation of “sandwich” complexes in the test“T” line creates a visible signal.Positive ResultCapture AntigenCarrier ProteinGold Nano ParticleCapture AntibodyGeneric AntigenAntibody of InterestGeneric AntibodySignal Amplification ProteinGold Nano ParticleAntigen of Interest23