Falcon Product Selection Guide - Corning Inc.
Falcon Product SelectionGuide
Falcon Product Selection GuideCorning Life SciencesGeneralInformationTable of ContentsGeneral InformationAdditional Falcon LabwareFalcon Brand Products . . . . . . . . . . . . . . . . . . . . . . . . . 2Bacteriological Petri Dishes . . . . . . . . . . . . . . . . . . . . . . 40Our Commitment to Quality . . . . . . . . . . . . . . . . . . . . . . 3Cell Strainers and Containers . . . . . . . . . . . . . . . . . . . . . 41Falcon CulturewareCell Culture Flasks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5Flasks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5Multi-Flasks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7Flask Caps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9CultureSlides . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10Cell Culture Dishes . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11Plates and Microplates . . . . . . . . . . . . . . . . . . . . . . . . . 13Cell Scrapers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16Library Storage Plates . . . . . . . . . . . . . . . . . . . . . . . . . . 42Falcon Pipets and Pipet ControllerSerological Pipets . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44Individually Wrapped Serological Pipets . . . . . . . . . . . . . . 45Bulk-Packaged Serological Pipets . . . . . . . . . . . . . . . . . . 46Aspirating Pipets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47Transfer Pipets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48Pipet Controller . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49In Vitro Fertilization Plasticware . . . . . . . . . . . . . . . . . . . 17Falcon TubesCorning Primaria Cultureware . . . . . . . . . . . . . . . . . . 19Round-Bottom Tubes . . . . . . . . . . . . . . . . . . . . . . . . . . 51Cell Culture Inserts . . . . . . . . . . . . . . . . . . . . . . . . . . . 21Conical Tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53Inserts and Companion Plates . . . . . . . . . . . . . . . . . . . 21Laboratory Aids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5524 Multiwell Insert Systems . . . . . . . . . . . . . . . . . . . . 2596 Multiwell Insert Systems . . . . . . . . . . . . . . . . . . . . 27Corning FluoroBlok Cell Culture Inserts . . . . . . . . . . . . 29Corning FluoroBlok 24 Multiwell Insert Systems . . . . . . . 31Corning FluoroBlok 96 Multiwell Insert Systems . . . . . . . 33Tube Size Identification Chart . . . . . . . . . . . . . . . . . . . 55Tube Chemical Resistance Chart . . . . . . . . . . . . . . . . . 56Characteristics of Falcon Plasticware . . . . . . . . . . . . . . . 57Rotor/Adapter Selection Guide . . . . . . . . . . . . . . . . . . 59Nomogram for Computing Relative Centrifugal Force . . . . 60Laboratory Aids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35Microplates Key Dimensions . . . . . . . . . . . . . . . . . . . . 35IndexWorking Volumes for Tissue Culture Vessels . . . . . . . . . . 37Catalog Number Index . . . . . . . . . . . . . . . . . . . . . . . . . 61Cell Record Worksheet . . . . . . . . . . . . . . . . . . . . . . . . 38www.corning.com/lifesciences1
Falcon Product Selection GuideCorning Life SciencesGeneralInformationFalcon Brand ProductsKnown the world over for the popular “Falcon Tubes”, the Falcon brand has set the standard for highquality disposable plasticware for more than 50 years. Falcon-branded liquid handling and cell cultureproducts are designed to advance research and are used in laboratories worldwide. Manufacturedfrom advanced bioanalytical-grade resins and extensively tested, Falcon plasticware ensures highquality and consistent, reliable results.Committed to delivering the right product at the right timeCorning Sales and Service Representatives are specialists who have the training and knowledge tohelp you make the right purchasing decisions. You can be confident they’ll direct you to the productsthat deliver the best value while meeting your research needs.Our Sales Representatives work in close partnerships with our distributors to ensure the availability ofall of our products and to keep you up-to-date on new products and technologies. They stay in touchwith you and the changing requirements of your research.Corning Scientific Support Representatives have laboratory experience and an in-depth understandingof our products and their applications. Based on their experience, they provide expert assistance inselecting the products that best meet the requirements of your research protocols.Our Customer Service Representatives provide the information you need to place and process yourorders accurately and efficiently. They make sure you get the products you need, when and where youneed them, whether they are through one of our authorized distributors or directly through Corning.Whether you need to place an order or have a special request or concern, depend on Corning Salesand Service Representatives.www.corning.com/lifesciences2
Falcon Product Selection GuideCorning Life SciencesGeneralInformationOur Commitment to QualityAll Falcon products are manufactured per current Good Manufacturing Practices (cGMP) and ISO9001 standard. The ISO certification verifies our facilities meet international quality system standards.The quality system is routinely audited by a notified body to ensure a work environment thatconsistently maintains the highest standards. ISO compliance gives our customers an added level ofassurance that Corning is committed to superior quality and continuous product improvement.Copies of our ISO and quality certificates can be obtained by contacting Corning Scientific Support at 1.978.442.2200 or e-mail ScientificSupport@corning.com.Corning facilities and the products they manufactureDurham, North CarolinaISO 9001 and EN ISO 13485 certified and cGMP (21 CFR 820) compliant Falcon flasks Falcon multiwell plates Falcon vented caps Falcon assay plates Falcon serological pipets Falcon bacteriological petri dishes Falcon aspirating pipets Falcon in vitro fertilization plasticware* Falcon specimen containers Corning Primaria flasks Falcon cell strainers Corning Primaria dishes Falcon cell culture inserts Corning Primaria plates Falcon cell culture dishesReynosa, MexicoISO 9001 and EN ISO 13485 certified and cGMP (21 CFR 820) compliant Falcon conical tubes Falcon round-bottom tubes*Falcon In Vitro Fertilization plasticware is CE marked in compliance with the European Medical Devices Directive93/42/EEC as amended by 2007/47/EC. Falcon IVF plasticware cleared as US Class II medical devices by the FDAper 21 CFR 884.www.corning.com/lifesciences3
Falcon Product Selection GuideCorning Life SciencesFalcon CulturewareTable of ContentsFlasks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5Multi-Flasks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7Flask Caps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9CultureSlides . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10Cell Culture Dishes . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11Plates and Microplates . . . . . . . . . . . . . . . . . . . . . . . . . 13Cell Scrapers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16In Vitro Fertilization Plasticware . . . . . . . . . . . . . . . . . . . 17Corning Primaria Cultureware . . . . . . . . . . . . . . . . . . 19Cell Culture Inserts . . . . . . . . . . . . . . . . . . . . . . . . . . . 21Inserts and Companion Plates . . . . . . . . . . . . . . . . . . . 2124 Multiwell Insert Systems . . . . . . . . . . . . . . . . . . . . 2596 Multiwell Insert Systems . . . . . . . . . . . . . . . . . . . . 27Corning FluoroBlok Cell Culture Inserts . . . . . . . . . . . . 29Corning FluoroBlok 24 Multiwell Insert Systems . . . . . . . 31Corning FluoroBlok 96 Multiwell Insert Systems . . . . . . . 33Laboratory Aids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35Microplates Key Dimensions . . . . . . . . . . . . . . . . . . . . 35Working Volumes for Tissue Culture Vessels . . . . . . . . . . 37Cell Record Worksheet . . . . . . . . . . . . . . . . . . . . . . . . 38Trust Falcon, the first name in cell cultureFalcon Cultureware was the first developed tissue culturetreated plasticware that enabled scientists to grow cells in vitro.www.corning.com/lifesciences4Falcon CulturewareCell Culture Flasks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Falcon Product Selection GuideCorning Life SciencesFalcon Cell Culture Flasks Vacuum-gas plasma tissue culture treatment providesconsistent cell attachment, spreading, and growth Choose standard tissue culture, Corning Primaria , or notFalcon Culturewaretissue culture-treated polystyrene growth surfaces to meetyour individual cell culture requirements Secure stacking provides stability from accidental tips or spills,and protection against contamination (canted neck flasks) Sterilized by gamma irradiation Nonpyrogenic tested to less than 0.1 EU/mL Volumetric graduations and writing patch Vented caps incorporate a 0.2 µm hydrophobic membraneto isolate the container it is placed on from the environmentwhile allowing gas exchange and reducing spills. Growth area and volume are nominal225 cm2 Flasks Innovative shape permits access to all corners with a pipetor scraper Unique Locking Incubation Position prevents caps fromfalling off or closing while in the open position Plug Seal or vented cap available Skirted, canted neck adds stability to neck area of flask Large frosted writing area Vertical graduations up to 400 mL 100 mL maximum horizontal working volume lineTissue culture-friendly packaging Convenient reseal tab on the bag Innovative bag materials that will not scuff or scratch theflask’s optical surface Double-wall bags provide increased sterility assurance Medical-style, peel-open bags assure that flask sterilityis maintained Recyclable bag material (low density polyethylene) Knife not required for case openingLow profile 150 cm2 Flasks Low profile for efficient stacking and incubator utilization Unique Locking Incubation Position prevents caps fromfalling off or closing while in the open position Precision engineered cap spins on quickly Innovative shape permits access to all corners with a pipetor cell scraper5www.corning.com/lifesciences
Falcon Product Selection GuideCorning Life SciencesFalcon Cell Culture Flasks Ordering InformationCat. No.SurfaceNeck StyleCap StyleQty/PkQty/CsStandard TC*Standard TCCantedCantedPlug SealVented101010010025 cm2 Flask, 50 mL353014353108353813353808Standard TCStandard TCCorning Primaria TCCorning Primaria TCCantedCantedCantedCantedPlug SealVentedPlug SealVented2020202020010020010025 cm2 Flask, 70 mL353082353109353009Standard TCStandard TCNon-TCCantedCantedCantedPlug SealVentedPlug Seal202020200100200Standard TCStraightPlug Seal5100Standard TCCorning Primaria TCCorning Primaria TCStandard TCStandard VentedPlug SealVentedPlug SealVentedPlug Seal555555100100100606060150 cm2 Flask, 600 mL355000355001Standard TCStandard TCCantedCantedPlug SealVented554040175 cm2 Flask, 750 mL353028353112Standard TCStandard TCStraightStraightPlug SealVented554040225 cm2 Flask, 800 mL353139353138Standard TCStandard TCCantedCantedPlug SealVented553030Neck StyleCap StyleQty/PkQty/Cs-Vented212-Vented18cm212.5Flask, 25 mL353018353107353824353810353135353136353133Falcon Cultureware75 cm2 Flask, 250 mL353024353110Falcon Cell Culture Multi-Flasks Ordering InformationCat. No.Surface525 cm2 Multi-Flask 3-Layer, up to 50 mL per layer**353143Standard TCcm2875Multi-Flask 5-Layer, up to 50 mL per layer**353144Standard TC*TC Tissue Culture** For more information on the Multi-Flasks, see page 6Tips Use Falcon non-Tissue Culture-treated cell culture flasksfor suspension cultures. For enhanced cell performance, Corning BioCoat Flasks are available with pre-applied matrix proteins, andCorning PureCoat Flasks are available with syntheticcoating (amine and carboxyl). For more information, lifesciences6
Corning Life SciencesFalcon Product Selection GuideFalcon Cell Culture Multi-Flasks Increase productivity by enabling to grow more cells faster and easier 3- or 5-layer formats provide 525 cm2 and 875 cm2 cell growth surface area, respectivelyFalcon CulturewareFeatures Even distribution of media across all layers for homogeneouscell growth Ability to mix cells and reagents in the Falcon Multi-Flasksaves time and reduces risk of contamination Flexible design allows to pour or aspirate/recover cells usinga pipet Consistent surface treatment for predictable scale-up Lot number printed on each flask for traceability Manufactured in compliance to cGMP standardsDesigned to fit your protocolFalcon Multi-Flasks offer the same footprint and the samereagent volumes and cell seeding densities per unit areaas 175 cm² flasks. With the same proven surface treatmentas all other Falcon flasks, it simplifies scale up.Improves cell culture productivityFalcon Multi-Flasks deliver a thoughtful design that simplifiescell culture workflow by eliminating multiple steps and reducingthe risk of contamination. Pipet access allows to aspirate to replenish media and recovercells without pouring The mixing port enables rapid mixing inside the vessel andallows to add cell suspension, transfection, or other reagentsdirectly to the flask. The port also enables uniform distributionof media and cells to facilitate homogeneous cell growth onall layers.More consistent cell growthFalcon Multi-Flasks’ even distribution of media, proven vacuumgas-plasma tissue culture surface treatment, and effectivegas exchange all combine to provide an optimal cell cultureenvironment. The result is high cell yield and a homogeneouscell population.7www.corning.com/lifesciences
Falcon Product Selection GuideCorning Life SciencesPredictable scale-upConsistent cell growth3002502006Falcon CulturewareCell Yield (x 10 ; mean SD)350150100500Falcon T-175 FlaskFalcon Multi-Flask(3-layer)Falcon Multi-Flask(5-layer)TopMiddleBottomT-175 (control)Cell Culture VesselExpected YieldActual Yield2Cells per unit area (x 10 ; cells/cm )0.560.40.30.20.10.0BHK-21LnCapHep-G2EcoPack 2-293Cell LineT-175Falcon Multi-Flask (3-layer)Falcon Multi-Flask (5-layer)Three and five times the number of BHK-21 cells were grown and recovered from 3- and 5-layer Falcon Multi-Flasks compared to T-175 flasks.Expected yield (left panel) was determined using mean cell yield fromcontrol T-175 flasks multiplied by three and five times for the 3- and5-layer Falcon Multi-Flasks, respectively. Cell yield per cm2 (right panel)was equivalent in 3- and 5-layer Multi-Flasks and T-175 flasks for BHK21, LnCap, Hep-G2 and EcoPack 2-293 cells.This figure illustrates uniform cell growth between layers of FalconMulti-Flasks. BHK-21 cells grown to 80% confluence in 3-layer FalconMulti-Flasks and control T-175 flasks were fixed and stained with crystalviolet. Control flasks and individual layers of the Falcon Multi-Flasks werecut and scanned.Falcon Cell Culture Multi-Flasks Ordering InformationCat. No.DescriptionFalcon Multi-Flasks3531433-Layer TC-treated3531445-Layer TC-treatedSurface Area (cm2)Cap StyleQty/Cs525 cm2875 cm2VentedVented128250Tips6Cell Yield (x 10 SD)200 Contact your local Corning office for custom coating -293CHO-M1MSC( Serum)MSC(- Serum)Cell LinesFalcon T-175 FlaskFalcon Multi-Flask (3-layer)Compatible with your cell linesDiverse cell lines and primary cultures (with and without serum) can begrown and efficiently recovered from Falcon Multi-Flask cell culture vessels. This graph illustrates the increased cell yield one can obtain fromFalcon Multi-Flasks as compared to control T-175 flasks for various celllines.www.corning.com/lifesciences8
Falcon Product Selection GuideCorning Life SciencesFalcon Flask CapsA variety of caps to suit your cell culture requirementsFalcon polyethylene plug seal caps provide a liquid-tight sealwhen closed and an open-incubation position for reliable gasexchange when partially opened. A reference bar on the outsideof the cap is aligned with the “Falcon” on the flask for preciseopen-incubation conditions.Convenient vented caps Ensure consistent gas exchange and reduce spills Polyethylene caps with an integral, hydrophobic 0.2 µm micro-porous membrane filter vent allow consistent gas exchangewhen the caps are in the closed position (see graph). Gasesrequired for cell growth and metabolism pass freely throughthe vent while microorganisms cannot. Falcon vented caps willnot wet out. The vented caps prevent media that can become trapped in apartially opened cap from blocking gas exchange. The caps willnot fall off in the incubator because of vibration.Falcon Flask Caps Ordering InformationCat. No.DescriptionLoose Plug-Seal Cap8.0Qty/CsVented Caps for Falcon Flasks354637For use on 25 cm2 Flasks100354638For use on 75 cm2 Flasks100354639For use on 175 cm2 Flasks50Closed Vented Cap7.87.6pHFalcon CulturewarePlug Seal caps7.47.27.06.86.601530456090120150180Time (minutes)pH equilibration using vented caps after flasks are placed in an incubator(175 cm2 Flasks, 5% CO2 incubator)Tips Corrugated cardboard boxes are a source of particulatesand associated microbial contamination and should not bekept in the tissue culture area. Corning Life Sciences has eliminated corrugated trays from most cases to reducepackage waste. You can further reduce contamination dueto corrugate by wiping the outside of any package or bagwith alcohol or an a ppropriate disinfectant before puttingit under the hood.9www.corning.com/lifesciences
Falcon Product Selection GuideCorning Life SciencesFalcon CultureSlides Safe and easy chamber removal for microscopic analysis Innovative sealing designFalcon CultureSlides for in situ analysisFalcon CulturewareFalcon CultureSlides allow you to culture cells and then analyzethem on a glass microscope slide. Cells are grown in a plasticchamber affixed to a specially prepared glass microscope slide.Cells can be fixed and stained in place without disruption of thecell monolayer. The chamber is easily and safely removed withan easy-to-use, disposable Safety Removal Tool.Designed for consistent cell culture results Specially cleaned and triple-rinsed glass slides Performance validated with HEp-2 and BAE cells Tested for 72-hour confluency w
Corning Life Sciences Falcon Product Selection Guide Falcon Cultureware Falcon Cultureware Trust Falcon, the first name in cell culture Falcon Cultureware was the first developed tissue culture-treated plasticware that en
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When designing a storage tank, it is necessary to meet the requirements of the design code (in this case, API 650), and also with all those requirements of the codes involved in the process of the tank. Some of them are listed below: API-RP 651: Cathodic Protection of Aboveground Petroleum Storage Tanks
