AMG: Micron(EVOS) User Guide - Thermo Fisher Scientific
Micron (EVOS)Digital Imaging SoftwareUser Guidea division of Westover Scientific, Inc.18421 Bothell-Everett Hwy.Suite 150Mill Creek, WA 98012Phone:Fax:E-mail:Web:(866) 614-4022 or (425) 368-0444(425) 368-0555info@amgmicro.comwww.amgmicro.com
MICRON (EVOS) User GuideCo n t e n t sMicron (EVOS) Features. 2System Requirements.2Software Installation . 3–6Uninstalling Previous Version.3Installing Software.3Driver Installation. 7–9Found New Hardware Wizard.7If you have the Micron(EVOS) Installation CD.8If you DO NOT have the Micron(EVOS) Installation CD. . .9Operation . 10–19Getting Started.10Main Window.10Adjusting the Image.11Capturing an Image.12Annotation and Measurement.12Submenu Options.15Select All.16Saving an Image.16Printing an Image.17Print Previewing an Image.17AutoSaving an Image.18Calibration.19Menus . . 21–30File Menu.21Edit Menu.22View Menu.22Tools Menu.23Annotation Menu.24Measurement Menu.26Auto Create Areas.28Window Menu.30Help Menu.30Toolbars & Icons . 31–34Main Toolbar.31Annotation Toolbar.32Measurement Toolbar.33Status Bar.34Troubleshooting . 35More Help.35Customer Service . 36Contact Information.36Warranty Information.36 2008 Westover Scientific, Inc. All rights reserved.
Micron (EVOS) FeaturesMicron (EVOS) FeaturesMicron is the Westover Scientific imaging software that lets you make thebest use of a camera attached to a microscope. Among Micron’s features are: Simple and quick installation Easy and intuitive user interface Hi-resolution 2048 x 1536 (EVOS) Frame rate of 48 MHz for Intel -based chips (24 MHz for all others) Can freeze images Saves images to disk files on the PC Recalls previously saved images for later review Prints images Annotates imagesSystem Requirements (Minimum)XX PC with Pentium III 1.0 GHz CPU or higherXX Windows 2000 (SP4) or XP Operating SystemXX 30 MB of hard drive space for application softwareXX One open USB 2.0 portXX 256 MB RAM2Micron(EVOS) User Guide
Software InstallationUninstalling Previous VersionBefore installing the Micron(EVOS) imaging software, remove any previouslyinstalled versions of Micron:1.Click the Start menu button.2.Select Programs Westover Scientific Uninstall Micron.The uninstall program will run and remove the previously installed version.Another method for removing Micron uses the Control Panel:1.Click the Start menu button.2.Click Settings Control Panel.3.Click Add or Remove Programs.4.Scroll through the list of installed programs and select Micron.5.Click Remove.Installing SoftwareTo install Micron(EVOS), exit all Windows programs you have running andplace the installation disk in your computer’s CD-ROM drive. Wait a fewseconds for the computer to recognize the new disk. The setup programshould start automatically.If the setup program starts automatically, skip down to step 5, below.If the setup program does not start automatically:1.Click the Start menu button.2.Click Run.3.In the Run dialog box, type:D:\setupassuming that D is your computer’s CD-ROM drive. If D is incorrect,substitute the appropriate drive letter.amgmicro.com3Micron(EVOS) User Guide
Software InstallationScreen 1Screen 2Screen 3WelcomeLicense AgreementSelect Destination Location4.Click OK. This should start the setup program that installs Micron.5.At the Welcome screen, click Next to continue to the LicenseAgreement screen.6.To install Micron, you must accept all the terms of the softwarelicense agreement. If you agree, click the “I accept the agreement”button to continue. If you do not agree, click “I do not accept theagreement” to cancel the installation.7.Click Next after you have agreed to continue.8.At the Select Destination Location screen, accept the defaultlocation and click Next, or select an alternate location where youwant Micron to be installed and click Next.4Micron(EVOS) User Guide
Software InstallationScreen 4Select Additional TasksScreen 5Ready to InstallScreen 6Informationamgmicro.com9.To create a desktop or quick launch icon for Micron, check the boxesand click Next to proceed.10.At the Ready to Install screen, click Install to continue. The Micronfiles will be copied to your computer.11.At the Information screen, take a moment to read the ReleaseNotes, then click Next to continue.5Micron(EVOS) User Guide
Software InstallationScreen 7Completing Setup12.At the Completing the Micron EVOS. screen, click Finish.tocomplete software installation. To open the User Guide (PDF), youmust have a PDF reader application installed.Note: Download a free copy of Adobe Reader from the URL ep2.html6Micron(EVOS) User Guide
Driver InstallationFound New Hardware WizardConnect the USB2 cable to EVOS (behind LCD panel) and to an open USB2port on the PC. Follow the directions below to complete installation of theUSB2 device drivers.USB2 requires device drivers that are not completely installed as part of theMicron software installation. Instead, they are installed by the Found NewHardware Wizard when you first plug the USB2 cable into the PC.Note: Also, if you move the USB2 cable to another USB2 port on your computer,the Found New Hardware Wizard will again appear, just as though you hadnot already installed the drivers.Once the USB2 cable is connected to the PC, the Found New HardwareWizard automatically starts to guide you through the driver installationprocess.Note: Wait for the wizard to start; it may take a few minutes.Screen 1amgmicro.comFound New Hardware Wizard1.At the Found New Hardware Wizard, select No, not this time, andclick Next to proceed.2.If you have the Micron(EVOS) installation CD, go to step 3a.If you DO NOT have the Micron(EVOS) installation CD, go to step 4a.7Micron(EVOS) User Guide
Driver InstallationScreen 3aInstall the software automaticallyIf you have the Micron(EVOS) Installation CD.3a.Screen 3bHardware Installation3b.Make sure the Micron(EVOS) installation CD is inserted in theCD drive and select Install the software automatically(Recommended) button, then click Next to continue.At the Hardware Installation screen, click Continue Anyway toproceed.Note: Westover Scientific has extensively tested Micron to verify itscompatibility with Windows . Proceed with confidence.Screen 3cCompleting the Found New Hardware Wizard3c.At the Completing the Found New Hardware Wizard, click Finish tocomplete driver installation.8Micron(EVOS) User Guide
Driver InstallationScreen 4aInstall the software automaticallyIf you DO NOT have the Micron(EVOS) Installation CD.4a.At the Found New Hardware Wizard screen, select Install from alist or specific location (Advanced) button, then click Next tocontinue.Screen 4bHardware Installation4b.When Micron(EVOS) was installed, the drivers were copied to theMicron installation directory, which, by default, is:C:\Program Files\Westover Scientific\Micron(EVOS)Select the Search for the best driver in these locations buttonand check the Include this location in the search box. Browse tothe directory above and click Next to continue.Screen 4cCompleting the Found New Hardware Wizard4c.At the Hardware Installation screen, click Continue Anyway toproceed.Note: Westover Scientific has extensively tested Micron to verify itscompatibility with Windows . Proceed with confidence.4d.amgmicro.comAt the Completing the Found New Hardware Wizard, click Finish tocomplete driver installation.9Micron(EVOS) User Guide
OperationGetting StartedBefore you get started, make sure. EVOS is powered on and operating properly; the latest version of Micron is installed on your PC; the USB2 cable is attached to EVOS and to a USB2 port on your PC.Note: You cannot tell by looking at a cable if it is USB or USB2, so make sureyou have the correct cable by checking any labels.Place a slide or specimen on the stage. Using the focus control knobs, bringthe image into focus.Main WindowLaunch/open Micron.Menu BarMain ToolbarImage WindowAnnotation ToolbarMeasurement ToolbarStatus Bar10Micron(EVOS) User Guide
OperationAdjust CameraAdjusting the ImageOn the View menu, click Adjust Camera (you can also click the AdjustCamera icon on the Main toolbar).Brightness SliderAdjusts the average brightness of the image between 0% and 100%.Contrast SliderAdjusts the spread of brightness of the image between 0% and 100%.Saturation SliderAdjusts the amount of color in the image between 0% (black and white) and100% (as colorful as possible).Hue SliderAdjusts the relative gains of red, green, and blue camera pixels according tothe location of the slider on the hue line.Note: Hue wraps around so that very small values of hue are nearly identical tolarge values of hue.Reset ButtonResets all image settings above to default (exact values/levels coming frommicroscope).Frame RateHow quickly an image updates on the screen. For Intel -based chips, theframe rate will default to 48 MHz, for all others the option for 24 MHz will beavailable.Note: If you have an older computer or the user-interface is not responsive, tryselecting the lower frame rate (24 MHz). Lower frame rates may also be preferredwith low-light images.amgmicro.com11Micron(EVOS) User Guide
OperationCapturing An ImageMain ToolbarTo capture the image on the PC screen, click either: Camera IconThe capture button on the camera, if the camera has one, orThe Camera icon on Micron’s Main toolbar:Note: The Camera icon has a lighter background when you are viewing livevideo.When you press the camera’s capture button or click Micron’s Camera icon,the Annotation and Measurement toolbars become active. You can thenannotate, print, and save the image you captured.To return to a live image, either.Auto Save click the Camera icon again, or click Live Video on the View menu.Annotation and MeasurementSeveral items on the Annotation and Measurement menus—as well as theiridentical counterparts on the Annotation and Measurement toolbars—letyou add lines, arrows, and text to the images you captured. You can also drawgeometric shapes to surround areas you want to highlight on the capturedimage.Note: You cannot add these features to a live image.Auto SaveAutomatically saves any captured image to file at the instant the image iscaptured (before any annotations or other changes are added to the image).The Auto Save feature is discussed in the AutoSaving an Image section (seepage 18).12Micron(EVOS) User Guide
OperationLive OverlayLive OverlayThis dialog box lets you superimpose a grid on the screen. You can select thenumber of rows and columns, and the color and thickness of the lines in thegrid.The Bitmap File option lets you call up a saved file and overlay it on top ofyour image. This feature is useful for video comparators.In this feature: The screen overlay must be a Windows bitmap (.bmp) file, so onlybitmaps or other image files—JPGs or PNGs—that can be converted tobitmap format can be loaded (Micron automatically converts a .jpg or.png file loaded in this feature to bitmap). If the bitmap file includes a transparency band (alpha band), thetransparency information is applied on a per-pixel basis. If theinformation is not supplied, the dominant color is taken as thetransparent background. Micron scales the bitmap to match the image size, so for best results setthe bitmap to match the Micron 4:3 aspect ratio. For example, if you arecreating an overlay file with Microsoft Paint, select Image Attributesand set the Width to 640 and the Height to 480.The Micron Annotation File option lets you call up a saved annotation fileand lays it over the current image. The file must be a .png file, and it musthave saved annotations in it.The Transparency feature governs how transparent or opaque the grid is.Note: For Live Overlay to appear correctly on your screen, you need a high-endgraphics or video card. Graphics cards that perform well with Live Overlay havethe following hardware support built into them: Support for YUV and “non-power of 2” Direct3D texture surfaces. The ability to StretchBlt from YUV to RGB DirectDraw surfaces. At least 16MB of video memory if multiple video streams are to beblended together. The actual amount of memory required is dependenton the image size of the video streams and resolution of the displaymode being used. Support for an RGB overlay or the ability to blend to a YUV overlaysurface. Hardware-accelerated video (support for DirectX Video Acceleration)decoding. High pixel-fill rates.Note: Live Overlay requires that the system monitor be set for a color depth ofat least 16 bits. Live Overlay cannot run if th
transparency information is applied on a per-pixel basis. If the information is not supplied, the dominant color is taken as the transparent background. Micron scales the bitmap to match the image size, so for best results set the bitmap to match the Micron 4:3 aspect ratio. For example, if you are
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EASIER. SMARTER. FASTER. with. EVOS Microscopes by AMG. WHY EVOS? EVOS is the must have system for microscopy, whether you're capturing images for publication, teaching or researching. From cell culture to complex protein analysis to multi-channel fluorescence imaging, EVOS microscopes are your solution for a variety of routine and specialty .
EASIER. SMARTER. FASTER. with. EVOS Microscopes by AMG. WHY EVOS? EVOS is the must have system for microscopy, whether you're capturing images for publication, teaching or researching. From cell culture to complex protein analysis to multi-channel fluorescence imaging,
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