C-9 And Other Microgravity Simulations Summary Report
NASA/TM-2010-216132C-9 and Other Microgravity SimulationsSummary ReportReport prepared bySpace Life Sciences DirectorateHuman Adaptation and Countermeasures DivisionNASA Johnson Space Center, HoustonNational Aeronautics andSpace AdministrationJohnson Space CenterHouston, TX 77058September 2010
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NASA/TM-2010-216132C-9 and Other Microgravity SimulationsSummary ReportReport prepared bySpace Life Sciences DirectorateHuman Adaptation and Countermeasures DivisionNASA Johnson Space Center, HoustonNational Aeronautics andSpace AdministrationJohnson Space CenterHouston, TX 77058September 2010
PREFACEThis document represents a summary of medical and scientific evaluations conducted aboard theC-9 and other NASA-contracted aircraft from June 2009 to June 2010. Included is a generaloverview of investigations manifested and coordinated by the Human Adaptation andCountermeasures Division. A collection of brief reports that describes tests conducted aboardNASA-sponsored aircraft follows the overview. Principal investigators and test engineerscontributed significantly to the content of the report, describing their particular experiment orhardware evaluation. Although this document follows general guidelines, each report formatmay vary to accommodate differences in experiment design and procedures. This documentconcludes with an appendix that provides background information concerning the NASA ReducedGravity Program.AcknowledgmentsThe Space Life Sciences Directorate gratefully acknowledges the work of Sharon Hecht,Jacqueline M. Reeves, and Elisabeth Spector for their outstanding editing support and contributions to the overall quality of this annual summary report.Available from:NASA Center for AeroSpace Information7115 Standard DriveHanover, MD 21076-1320Phone: 301-621-0390 orFax: 301-621-0134National Technical Information Service5285 Port Royal RoadSpringfield, VA 22161703-605-6000This report is also available in electronic form at http://ston.jsc.nasa.gov/collections/TRS/
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ContentsPageOverview of Reduced Gravity Flight Activities Sponsored by theHuman Adaptation and Countermeasures Division.1Medical and Scientific Evaluations during Parabolic Flights .2Education Outreach Program – Effects of Altered Gravity on Cellular Function .3High-Accuracy Eye-movement Monitor .12FASTRACK Program – Antimicrobial Polymers Project .16Education Outreach Program – Space Motion Sickness and the SemicircularCanals of the Inner Ear .33Appendix . A-1Background Information about the C-9 and NASA Reduced Gravity Program . A-2ii
a2CrO4NSTAPPBSPCAmerican Chemical SocietyAir Force Research Laboratorysilversilver fluorideAntimicrobial Materials for Microgravity Environments Test Equipment DataPackageAdvanced Materials Processing Programacridine orangeacridine orange direct countAmerican Society for Testing and Materialsadenosine triphosphateAdvanced Water Recovery System Development FacilityBiological Research in a CanisterBiological Research in a Canister-Light-emitting Diodebiological safety cabinetchange data capturecarbon dioxideCommittee for the Protection of Human Subjectschromiumdeoxyribonucleic acidenvironmental control and life supportengineered roughness indexenvironmental scanning electron microscopyethylene oxideFacilitated Access to Space Environment for Technology Development andTrainingfluorodecyl polyhedral oligomeric silsesquioxanesheterotrophic plate countinductively coupled plasma atomic emission spectroscopyintegrated phase planningInstitutional Review Boardinverse Sharklet International Space StationJohnson Space CenterKennedy Space Centerlive/deadlaunch services support contractmathematics, engineering, science, and achievementmodified Petri dish fixation unitsodium chromateNational Science Teachers Associationpilin peptidephosphate buffered salinepolycarbonateiii
PDFUPDMSPEPETPOSSppbQGA RGOSASSEMSMSMSSPDFUSSTiTSBUSMUVWADPetri dish fixation unitpolydimethylsiloxanepolyethylenepolyethylene terephthalatepolyhedral oligomeric silsesquioxanesparts per billionQuench-Gone AqueousReduced Gravity Officespace adaptation syndromescanning electron microscopysmoothspace motion sicknessstandard Petri dish fixation unitstainless steeltitaniumTrypticase Soy BrothUniversity of Southern Maineultravioletwork authorization documentiv
Overview of Reduced Gravity Flight ActivitiesSponsored by the Human Adaptation and Countermeasures DivisionAs a summary for the year, 4 weeks were specifically reserved for flights sponsored from June2009 to June 2010. Seven flights with approximately 32 parabolas per flight were completed andthe average duration of each flight was 2.1 hours. The Reduced Gravity Program coordinatorassisted principal investigators and test engineers of 7 different experiments and hardwareevaluations in meeting the necessary requirements for flying aboard the C-9 or another NASAcontracted aircraft and in obtaining the required seating and floor space. Support was provided tothe Education Outreach Program during weeks in June and August 2009 and in April 2010. Alarge ground crew from the respective academic institutions supported the in-flight experiments.The number of seats supported and number of different tests flown by flight week are providedbelow:Flight Week2009June 11–12August 11–142010April 29–30SeatsNo. Tests FlownSponsor63514Education Outreach ProgramFASTRACK102Education Outreach ProgramFurther flights will be added throughout the remainder of calendar year 2010 to accommodatecustomers as needs arise.1
Medical and Scientific Evaluations during Parabolic Flights2
TITLEEducation Outreach Program –Effects of Altered Gravity on Cellular FunctionFLIGHT DATESJune 11–12, 2009PRINCIPAL INVETIGATORJohn Pierce Wise, Sr., PhD, University of Southern Maine (USM), Portland, MECOINVESTIGATORSJohn P Wise, USMMichael Browne, USMJane McKay, USMJennifer Brown, USMMatthew Braun, USMJames Wise, USMCatherine Wise, USMRyan Duffy, USMEben Estell, USMSandra Wise, USMKellie Joyce, USMMichael Mason, PhD, USM3
GOALTo determine the effects of altered gravity on cellular function.OBJECTIVESTo determine how altered gravity affects cellular function, we looked at three specific objectives:1. Determine whether human genotoxic agents cause more damage to cells and deoxyribonucleic acid (DNA) in altered gravity than in normal gravity. The effects of alteredgravity on the potency of human genotoxicants are not well understood. Last year we foundthat altered gravity increases damage induced by genotoxic chromate. Thus, this specificobjective is to confirm the effects of altered gravity on DNA damage in comparison tonormal gravity using an assay for chromosome damage.2. Determine whether the change in the amount of chromosome damage is due to an increase incellular uptake of chemicals by facilitated diffusion and phagocytosis in altered gravity: Theability of cells to take up chemicals in altered gravity is not well understood. This objective isto determine the effects of altered gravity on facilitated diffusion and phagocytosis usingassays for ion uptake and particle internalization.3. Determine whether the change in the amount of chromosome damage is due to inhibition ofDNA repair mechanisms by altered gravity. The effects of altered gravity on DNA repairmechanisms are not well understood. This objective is to determine the effects of alteredgravity on DNA repair relative to normal gravity, using an assay for DNA double-strandbreaks.METHODS AND MATERIALSOverviewThis hypothesis was tested by measuring the effects of known genotoxic chemicals in alteredgravity (flight), and comparing the results to identical tests conducted in normal gravity (ground).To keep the cells alive, we took a mini-oven (kept at human body temperature 37 C [98 F]) onboard the plane with us. The mini-oven was secured to the floor of the plane using a structure designed by SpaceWorks, Inc. (Grand Junction, CO). A cooler wasattached on top of this structure to stop a few experiments at theend of flight. We used this cooler on our first flight but decidedit was not important for this year’s experiment. Our task onboard the plane was to monitor the temperature and power.Collectively, the cooler-oven system (figure 1) worked verywell for our experiments.Figure 1. The “ rig” used to maintain the cells in flight.Cells and Cell CultureThe human lung cells were developed in the Wise Laboratory(Blankenship et al., 1997). These cells are fibroblasts immortalized with telomerase. Thetelomerase serves to extend the fibroblasts’ life spans, but the cells otherwise function normally4
and maintain normal responses to metals (Blankenship et al., 1997). The cells, which were maintained as adherent subconfluent monolayers (figure 2), were seeded48 h before treatment to allow them to settle and resume normal growth.For our experiments the cells were grown in T-25 flasks (figure 3A) andon sealed slide chambers (figure 3B). All experiments were conductedon logarithmically growing cells. There is little concern for the lack ofcarbon dioxide (CO2) gas exchange (an essential compound for cellgrowth) during the flight because the length of time without CO2exchange is insignificant.Figure 2. Human lung fibroblast cells under microscope.A – FlaskB – Slide chamberFigure 3. Pictures of cell culture tools.TreatmentsSodium chromate (Na2CrO4) (CAS #7775-11-3, American Chemical Society (ACS) reagentminimum 98% purity) was used as a soluble hexavalent chromium salt, Cr(VI). Solutions ofNa2CrO4 were prepared by: (1) measuring the desired amount of Na2CrO4; (2) dissolving theNa2CrO4 in double distilled water; and (3) filtering the solution through a 0.2-µm filter to sterilizethe solution. We used 5 to 20-µM concentrations of Na2CrO4 as our laboratory has employedthese methods with success (Xie et al., 2004).We treated cells for varying times, as indicated under each specific objective. All treatmentswere done at the Bioscience Core Laboratory at the NASA Johnson Space Center before flight.Our experiments focused on using vitamin C and cold temperature to stop the treatments. Forrepair experiments, we used vitamin C to stop the Na2CrO4 treatment, allowing the cells’ repairmechanisms to begin, because Na2CrO4 is a form of Cr(VI). Cr(VI) ions can enter the cell withthe help of a protein in the plasma membrane (Wise et al., 2004). By contrast, trivalent chromiumCr(III) ions cannot enter the cell (Wise et al., 2004). By adding vitamin C, the Cr(VI) ions arereduced to Cr(III) ions outside the cell, thus preventing further chromium (Cr) uptake. Therefore,vitamin C prevents Cr ions from getting into the cell and causing more DNA damage (Xie et al.,2005; Crawford-Young, 2006; Tischler and Morey-Holton, 1993). Not only does this approachprevent uptake, but our laboratory has shown that this approach also prevents chromosomedamage after Na2CrO4 exposure (Crawford-Young, 2006; Tischler and Morey-Holton, 1993).Vitamin C was dissolved in double distilled water before it was sterilized by filtering through a0.2-µm filter. We used a 2-µM vitamin C co-treatment as our laboratory found that thisconcentration maximizes the amount of chromate reduction without inducing cytotoxicity(Crawford-Young, 2006).5
The results of these experiments have implications for protecting the health of astronauts goingon extended missions to the International Space Station (ISS), the Moon, and, eventually, the outerreaches of the universe. These results will help determine how occupational exposure limits tohazardous materials in reduced gravity compare to the occupational exposure limits in normalgravity. Further, the results may impact how NASA engineers design future space equipmentand stations (ie, with or without the same materials, and in the same or different amounts to limitexposure).Specific Objective 1Our first specific objective was to determine whether human genotoxic agents damage cells andDNA more in altered than in normal gravity. The focus of this objective was to measure theeffects of altered gravity on DNA damage caused by exposure to chemicals. We considered theability of chemicals to induce chromosome damage in normal, hyper-, and microgravity. Bytreating cells according to the schedule in Table 1, we measured the amount of chromosomalabnormalities produced in metaphase cells after Na2CrO4 exposure using the Wise Laboratory’spublished methods for chromosome damage (Xie et al., 2004). We measured the amount of DNAdouble-strand breaks using immunofluorescence of H2A.X foci, as each focus is considered torepresent one DNA double-strand break. We also used the Wise Laboratory’s published methodsfor H2A.X foci formation.Table 1. Outline of Experiments for Specific Objective 1ExperimentChromosomedamagePurposeConfirm thatchromosome damageis increased by alteredgravityTreatmentNa2CrO4 (0, 5, 10,20 µM)Procedure Treat cells 1 h before takeoff Allow cells to incubate in warm environmenton plane Harvest 1 h after landingDNA doublestrand breaksDetermine whetherDNA breaks are alsoincreased by alteredgravityNa2CrO4 (0, 5, 10,20 µM) Treat cells 1 h before takeoff Allow cells to incubate in warm environmenton plane Harvest 1 h after landingCells were treated on the ground and then harvested shortly after landing, as described in Table1. After the experiments were completed and the cells harvested, the cells were prepared to besent back to Maine for microscopic analysis in the Wise Laboratory. We are analyzing 100metaphases per dose, and the results will be expressed as percentage of metaphases with damageand total damage in 100 metaphases. We predict that altered gravity will result in an increase inthe amount of DNA damage and the percentage of metaphases with damage. For DNA doublestrand breaks, cells were harvested at the cessation of the experiments and preserved for fociformation measurement before they were shipped to the Wise Laboratory for analysis on aconfocal microscope. We will analyze 50 cells per dose and the results will be expressed asaverage number of foci per cell. We predict that altered gravity will increase the level of DNAdouble-strand breaks.Specific Objective 2Our second specific objective was to determine whether the change in the amount ofchromosome damage is due to an increase in the cellular uptake of chemicals by facilitateddiffusion and phagocytosis in altered gravity. We tested the effects of altered gravity on cellularuptake of chemicals. We measured ion uptake and particle internalization in normal and altered6
gravity, and treated cells according to the schedule in Table 2. We then measured the amount ofCr ion inside the cell after a 4-h exposure to Na2CrO4 using the Wise Laboratory’s publishedmethods for inductively coupled plasma atomic emission spectroscopy (ICP-AES) (Holmes et al.,2005). Cells were treated on the ground and harvested shortly after landing, as described in Table2. After the experiments were completed, cells were frozen and shipped to the Wise Laboratory.There, cells will be analyzed for uptake using ICP-AES (ion uptake) or transmission electronmicroscopy. Data will be presented as µM Cr in the intracellular and extracellular environmentand percentage of cells with internalized particles. We predict that altered gravity will resultincrease the uptake of ions, thereby increasing the frequency of DNA and chromosome damage.Table 2. Outline of Experiments for Specific Objective 2ExperimentCr ion uptakePurposeDetermine the effects ofaltered gravity on the uptakeof Cr ions to compare withthe DNA damage studies inSpecific Objective 1TreatmentNa2CrO4(0, 5, 10, 20 µM)Procedure Treat cells 1 h before takeoff Allow cells to incubate in warmenvironment on plane Harvest 1 h after landingSpecific Objective 3Our third specific objective was to determine whether the change in the amount of chromosomedamage is due to an inhibition of DNA repair mechanisms by altered gravity. In this objective,we tested the effects of altered gravity on DNA repair mechanisms in an altered gravityenvironment. We measured DNA double-strand breaks using the immunofluorescence of H2A.Xfoci, as each focus is considered to represent one DNA double-strand break. We treated cells andallowed for a repair time according to the schedule in Table 3. We then measured the averagenumber of H2A.X foci per cell after Na2CrO4 exposure using the Wise Laboratory’s publishedmethods for H2A.X foci formation. After the experiments were complete, the cells were harvestedand preserved for foci formation measurement before they were shipped to the Wise Laboratoryfor analysis by confocal microscope. We will analyze 50 cells per dose and the results will beexpressed as average number of foci per cell. We predict that altered gravity will inhibit DNArepair mechanisms, resulting in a higher number of DNA double-strand breaks.Table 3. Outline of Methods of Specific Objective 3ExperimentDNA repair:4-h treatmentpreflightDNA repair:4-h treatmentpreflight 4-h repairrecoveryDNA repair:4-h treatmentpreflight 24-h repairrecoveryPurposeDetermine amount of damage incells before flightTreatmentNa2CrO4(0, 5, 10, 20 µM)Procedure Treat 5 h before takeoff Harvest 1 h before flightDetermine effects of alteredgravity on recovery of DNAdamageEqual treatment/recoverytime intervals Treat 5 h before takeoff Add vitamin C 1 h beforetakeoff Keep in incubator for flightduration Harvest 1 h after landing Treat 5 h before take-off Add Vitamin C 1 hourbefore takeoff Keep in incubator for flightduration Store in incubator for 22 h Harvest after 24-h recoveryDetermine effects of alteredgravity on recovery of DNAdamage after 4-h treatmentinterval over standard recoveryinterval, beginning on flightNa2CrO4(0, 5, 10, 20 µM)Short treatment time;standard recovery time,beginning in altered gravityNa2CrO4(0, 5, 10, 20 µM)7
RESULTSWe do not have completed data from this year’s experiments because it takes several weeks(sometimes months) to complete data analysis and we are currently analyzing our data. Theresults shown in figures 4 and 5 are based on last year’s experiments. We anticipate similarresults from this year’s experiment.Total Damage in 100 centration (uM)Figure 4. Chromosome damage resulting from Na2CrO4 treatments. The data show thereis a much higher incidence of chromosome damage in flight than on the ground. Forexample; at 10 µM, there are 43 chromosome aberrations per 100 metaphases on the groundand 92 in flight.6000Intracellular Concentration centration (uM)Figure 5. Cr uptake resulting from Na2CrO4 treatments at varying concentrations. Unlikewhat we expected to find, the data show there is a much lower Cr uptake in flight than onthe ground. For example: at 10 µM, the uptake is 1,773 µM in flight and 4,874 µM on theground.Now, if we take the intracellular Cr concentrations that we found in the uptake experiments andgraph them against the total amount of chromosome damage, we can more accurately demonstratethe effects of altered gravity, as shown in figure 6.8
Total Damage in 100 400050006000Concentration (uM)Figure 6. Chromosome damage resulting from the intracellular Cr concentration. Thegraph illustrates the following: (1) flight increases the rate at which chromosome damageoccurs; (2) there is much more damage in flight than on the ground (eg, at 1773 µM, thereare approximately 23 aberrations in the ground experiments; in flight, there are 92).DISCUSSIONBased on the results from our chromosome damage experiments, a combination of hyper- andmicrogravity causes an increase in the number of chromosomal aberrations induced by Na2CrO4.We used three concentrations of Na2CrO4 (5, 10, and 20 µM) and a negative control (0 µM) toconsider a dose response. Our results showed that altered gravity dramatically increased chromosome damage inducing two to three times the total amount of damage present in 100 metaphasecells and in the percentage of metaphase cells with damage. Analyzing the data, we observed thatthe total amount of chromosome damage and the percentage of damaged metaphase cells inaltered gravity drops at doses between 10 and 20 µM, which is most likely due to an increase incell death removing severely damaged cells from the population.Results from our experiments to determine the effects of altered gravity on cellular uptake ofchemicals indicate there is less uptake of chemicals in flight than on the ground. Again, we usedthree concentrations of Na2CrO4 (5, 10, and 20 µM) and a negative control (0 µM). Unlike ourhypothesis, our results indicated that altered gravity inhibited a cell’s ability to take up Cr(VI)ions. We used ICP-AES to analyze our samples, and found the normal gravity samples hadapproximately twice the number of Cr ions as the altered gravity samples. We observed a dropbetween the 10 and 20 µM treatments (similar to what we saw in the chromosome damageexperiments), and believe this drop to be a result of altered gravity increasing the occurrence ofapoptosis.Last year’s results to determine the effects of hyper- and microgravity on DNA repair mechanisms are incomplete because the antibody needed to analyze these slides failed. During DNArepair, a protein called H2A.X responds by accumulating around damaged DNA strands and initiating the repair process. We detected the H2A.X by adding an antibody that binds only to theprotein; this antibody is fluorescent and can be detected using a green light filter under a microscope. We conducted similar experiments at our laboratory to determine how long it would takefor DNA repair to occur. After treating cells with 10-µM Na2CrO4 for 4 h before co-treating thenwith vitamin C for 4, 6, and 24 h, we observed approximately half of the foci after a 4-h repair9
period. We did the same set of experiments with a 20-µM treatment, but did not see any repairuntil after 24 h. Thus, we expect to be able to find some significant results from this year’sexperiments.CONCLUSIONOur hypothesis predicted that due to the known morphological alterations cells undergo in zerogravity (Crawford-Young, 2006; Tischler and Morey-Holton, 1993), the altered-gravity environment that astronauts experience will have implications on cellular function, such that chemicallyinduced genotoxicity will be exacerbated. We hypothesized that this effect would result from anincrease in cellular uptake of chemicals and/or inhibition of DNA repair mechanisms. After analyzing the results from our chromosome damage experiments, we confirmed our hypothesis thataltered gravity exacerbates chemically induced genotoxicity. However, altered gravity does notincrease the amount of background damage, which means that flight alone is not genotoxic. Our results indicate that crewmembers exposed to genotoxic chemicals in flight could be at greater riskthan expected. We predicted this effect would be a result of the increased cellular uptake of ions,but our data proved our hypothesis incorrect. Instead, we found the cellular uptake of ions to bedrastically inhibited. We anticipate the data from this year’s experiment to show the same results,and indicate whether the exacerbated genotoxicity is due to inhibited DNA repair mechanisms orsome other unknown factor.REFERENCES1. Wise SS, Elmore LW, Holt SE, et al. Telomerase-Mediated Lifespan Extension of HumanBronchial Cells Does Not Affect Hexavalent Chromium-Induced Cytotoxicity or Genotoxicity.Mol Cell Biochem, 2004;255:103-111.2. Wise JP Sr, Wise SS, Little JE. The Cytotoxicity and Genotoxicity of Particulate and SolubleHexavalent Chromium in Human Lung Cells. Mutat Res. 2002;517:221-229.3. Costa M, Klein CB. Toxicity and Carcinogenicity of Chromium Compounds in Humans. CritRev Toxicol. 2006; 36.2:155-163.4. Blankenship LJ, Carlisle DL, Wise JP Sr, Orenstein JM, Dye LE III, Patierno SR. Inductionof Apoptotic Cell Death by Particulate Lead Chromate: Differential Effects of Vitamins Cand E on Genotoxicity and Survival. Toxicol Appl Pharmacol. 1997;146:270-280.5. Xie H, Holmes AL, Wise SS, Gordon N, Wise JP Sr. Lead Chromate-Induced ChromosomeDamage Requires Extracellular Dissolution to Liberate Chromium Ions but Does Not RequireParticle Internalization or Intracellular Dissolution. Chem Res Toxicol. 2004;17.10:1362-1367.6. Wise SS, Holmes AL, Ketterer ME, et al. Chromium Is the Proximate Clastogenic Speciesfor Lead Chromate-Induced Clastogenicity in Human Bronchial Cells. Mutat. Res. 2004;560:79-89.7. Xie H, Wise SS, Holmes AL, et al. Carcinogenic Lead Chromate Induces DNA DoubleStrand Breaks in Human Lung Cells. Mutat. Res. 2005;586(2):160-172.8. Crawford-Young SJ. Effects of Microgravity on Cell Cytoskeleton and Embryogenesis. Int JDev Biol. 200650:183-191.9. Tischler ME, Morey-Holton E. Space Life Sciences Research: The Importance of Long-TermSpace Experiments. Washington, DC: NASA Headquarters; 1993; NASA-TM-4502.10. Holmes AL, Wise SS, Xie H, Gordon N, Thompson WD, Wise JP Sr. Lead ions do notcause human lung cells to escape chromate-induced cytotoxicity. Toxicol Appl Pharmacol.2005;203:167-176.10
PHOTOGRAPHSJSC2009E135194 to 137339JSC2009E137558 to JSC2009E137559JSC2009E137577 to JSC2009E137578JSC2009E137598 to JSC2009E137600JSC2009E137631 to JSC2009E137649VIDEO Zero G flight week 6/5 – 6/12, 2009 Master: 743649Videos are available from Imagery and Publications Office (GS4), NASA Johnson Space Center(JSC).CONTACT INFORMATIONJohn P. Wise, Jr.john.p.wise@maine.eduDr. John P. Wise, Sr.john.wise@maine.eduDr. Michael Masonmmason@umche.maine.edu11
TITLEHigh-Accuracy Eye-Movement MonitorFLIGHT DATESAugust 11–12, 2009PRINCIPAL INVESTIGATORMark Shelhamer, Johns Hopkins University, School of Medicine, Baltimore, MDCOINVESTIGATORSKara Beaton, Johns Hopkins University, School of Medicine, Baltimore, MDAaron Wong, Johns Hopkins University, School of Medicine, Baltimore, MDDale Roberts, Johns Hopkins University, School of Medicine, Baltimore, MDMichael Schubert, Johns Hopkins University, School of Medicine, Baltimore, MD12
GOAL AND OBJECTIVESTo validate, in a realistic space analog environment, the performance of a device for accuratehigh-resolution measurements of eye movements during head and body motions. This device canaid future research programs into human sensorimotor adaptation to spaceflight.INTRODUCTIONThe control of eye movements represents one of the most basic and fundamental motor controlsystems of the human brain, combining information from both low-level motion transducers inthe inner ear (t
NASA/TM-2010-216132 . C-9 and Other Microgravity Simulations . Summary Report . Report prepared by . Space Life Sciences Di
The purpose of this curriculum supplement guide is to define and explain microgravity and show how microgravity can help us learn about the phenomena of our world. The front section of the guide is designed to provide teachers of science, mathematics, and technology at many levels with a foundation in microgravity science and applications.
Microgravity — A Teacher’s Guide with Activities in Science, Mathematics, and Technology, EG-1997-08-110-HQ, Education Standards Grades 5–8 ( ), 9–12 ( ) 46 Skylab, America’s first space station. Microgravity Science Space Flights Until the mid-20th century, gravity was an unavoidable aspect of research and technology.
MASER, and the Japanese TR-1A can provide 6 min of microgravity (with an apogee of about 250 km), while 15 minutes can be attained with the Swedish MAXUS. Orbital Flights can provide a good level of microgravity: 10-4 to 10-5 g, with the g-jitter depending mainly on the crew movements in
What is CCM-Wound Repair? Effect of Microgravity on Wound Repair: In Vitro Model of New Blood Vessel (CCM-Wound Repair) was an experiment that utilized the CCM
The cardiovascular system involves many subsystems that interact with each other and with other physiological sys-tems to insure system homeostasis. Mathematical modeling can play an important role in analyzing these complex interactions and, in particular, in analyzing the cardiovas-cular system and its response to the stress of microgravity.
On Earth muscles and bones support the body as we move against the force of gravity. In space’s microgravity environment, muscles and bones are not needed to support the body. Without use, bones and muscles become weaker. Astronauts follow an exercise program to keep their muscles and
physics, fundamental physics, and materials science) and work conducted on behalf of the MRP’s acceleration measurement, glovebox, and technology programs are managed by the MRPO and implemented at the following
Automotive Council The Advanced Propulsion Centre Thermal Efficiency and System Efficiency Spokes, supported by an expert Steering Group, helped to shape the roadmap before and after the workshop. Pre-event Common Assumptions Briefing Breakout Sessions Collective Briefing Process Post-Event Debrief Pre-Event Email 1 day workshop with 45 attendees Post-Event Email Thermal Propulsion Systems .
