Cell Culture Module: Effect Of Microgravity On Wound Repair
Cell Culture Module:Effect of Microgravity onWound RepairIn Vitro Model of New Blood VesselPresentation by Katherine and Lauren
What is the Cell Culture Module?The Cell Culture Module (CCM) is a completely automaticcell culture system designed to study the effects ofmicrogravity at the cellular and embryonic levels. It hasbeen in operation since 1996.
What is CCM-Wound Repair?Effect of Microgravity on Wound Repair: In VitroModel of New Blood Vessel (CCM-Wound Repair) was an experiment that utilized the CCM flew on International Space Station Expedition 15 lasted from April to October 2007CCM-Wound Repair's Principal Investigators were:—and—Dr. Stuart K. WilliamsDr. James B. Hoying
What is the ISS?The International Space Station is a satellite createdcollaboratively by 15 space agencies around the world (NASAincluded). It serves as a space laboratory, in whichexperiments like CCM-Wound Repair are performed.Since its launch inNovember 2000, the ISShas seen. over 200 astronauts over 600 experiments and 29 expeditions!Just how big is "big"? The ISS is the size of afootball field! That's 120 yards across! Italso has nearly an acre of solar panels.
Research ObjectivesThe ProblemMicrogravity has been shown to be detrimental to wound repair.Astronauts injured in space have a harder time healing.The Questions How does microgravity affectangiogenesis (thedevelopment of new bloodvessels)? How can these effects bemitigated?The Hypotheses The cells cultured inmicrogravity and the cellscultured on earth will differ ingene expression. Blood vessel developmentwill be impaired in cellscultured in microgravity. Cells injected with growthhormone will grow faster.
Experimental MethodsBefore the flight, endothelialcells (see picture and nextslide) were seeded ontobioreactors, devices capable ofsupporting biological systems(the system in this case beingthe cells and new vesselsresulting from angiogenesis).The cells were perused, orsustained and allowed to growby submergence in fluid. Halfthe samples were also injectedwith VEG-F, a growth hormone.During the flight, allsamples were fixed, ortreated to prevent decay.a diagram of a blood vessel
Adipose-derived Stem Cells (ADSCs)CCM-Wound Repair usedADSCs to generate endothelialcells for the experiment.Adult Stem Cells can bethought of as "template" cells—they can differentiate, ortransform, into other types ofcells, a process involved inorgan regeneration. ADSCs inparticular come from fat tissueand can differentiate into bone,muscle, nerves, blood vessels,etc. ADSCs may also speed upangiogenesis.an adipose tissue sample
Experimental ResultsAfter the flight, thesamples were returned toDr. Williams and Dr. Hoyingfor comparison withsamples from earth.Analysis is not yet done.Researchers are currentlyworking to characterize themicrogravity-induced stresseson the samples.Expected ApplicationsMicrogravity provides unique conditions for understanding the tissuedeath (necrosis) that occurs following serious injuries. In space, the results of the CCM-Wound Repair will helpresearchers treat the damaging effects of microgravityon wound repair and angiogenesis. On Earth, the results will provide a model by which tolearn more about angiogenesis and prevent tissuenecrosis and limb loss.
ReflectionsWe chose CCM-Wound Repair because we wereinterested in learning about a biology-based experimentand wound repair sounded like a fascinating topic. Theresults of this experiment don't seem to have beenpublished yet, but it will be exciting to learn what becameof this experiment. By doing this project, the enormity ofthe ISS was really put into perspective. We also learnedabout cell repair systems, bioreactors, adipose-derivedstem cells, and gained a general awareness as to theexperiments on the ISS.
Bibliography"Cell Culture Module - Effect of Microgravity on Wound Repair: In Vitro Model of New Blood Vessel Development (CCMWound Repair)." NASA. NASA, 10 Jun 11. Web. 30 Sep 2011. http://www.nasa.gov/mission pages/station/research/experiments/CCM-Wound Repair.html ."Cell Culture Module (CCM)." Life Into Space: Space Life Sciences Research. NASA, 2005. Web. 19 Oct 2011. http://lis.arc.nasa.gov/lis3/Hardware Appendix/CCM.html ."Explore Your Future: Store Your Adult Stem Cells Today." Advanced Minimally-Invasive Surgery. Advanced Minimally InvasiveSurgery, LLC, n.d. Web. 31 Oct 2011. derived-adult-stem-cells.asp ."International Space Station." NASA. NASA, n.d. Web. 31 Oct 2011. http://www.nasa.gov/mission pages/station/main/index.html ."The International Space Station." High-Tech Science. High-Tech Productions, n.d. Web. 7 Nov 2011. http://www.hightechscience.org/iss.htm ."White Adipose Tissue and Vasculature" Photograph. University of Delaware Biology Department. Web. 8 Nov 2011. ge/ca/wav.GIF ."Stem Cell 2." Photograph. Total Cosmetrix, Web. 8 Nov 2011. g ."International Space Station." Merriam-Webster Visual Dictionary Online. QA International, 2011. Web. utics/international-space-station.php ."Research: Innovation in Science and New Materials." Omega Genesis. OmegaGenesis, 2009. Web. 8 Nov 2011. http://www.omegagenesis.com/html/Research.html .Evans, Cynthia A., Julia A. Robinson, Judy Tate-Brown, Tracy Thumm, Jessica Crespo-Richey, David Baumann, and JenniferRhatigan, ed. and comp.International Space Station Science Research Accomplishments During the Assembly Years: An Analysis ofResults from 2000-2008 . Revision A. Houston: NASA, 2009. 125. Web. 11 Nov. 2011. http://www.nasa.gov/pdf/389388main ISSScience Report 20090030907.pdf .Momoko Yoshimoto, and Movin C. Yoder. Relationship between endothelial cells and blood cells. 2009. Graphic. Nature. Web. 13Nov 2011. g tab/457801a F1.html .
What is CCM-Wound Repair? Effect of Microgravity on Wound Repair: In Vitro Model of New Blood Vessel (CCM-Wound Repair) was an experiment that utilized the CCM
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