The MMO-MUG Test
A Newsletter for Ohio’s Public Drinking Water SystemsSummer 2015Volume 8, Issue 1The MMO-MUG TestCritical Documents that Make or Break ResultsOhio EPA’s microbiological certification program requires extensivedocumentation to ensure reliable laboratory data. Analysts certified to testdrinking water samples by MMO-MUG must have a thoroughunderstanding of the microbiological records associated with the drinkingwater program and how the information fits together to support accurateresults. Two documents discussed in this article, the Microbiological TestData Sheet (bench sheet) and the Incubator Temperature Records, aredirectly linked to the analytical process of this method.The information recorded on the bench sheets and in the incubatortemperature records are tied to the analytical process and document keyquality control components of the test. The data recorded in both logsdirectly impacts a sample’s validity. Together, these two documents show asnapshot of the entire analysis and demonstrate that the entire analyticalprocess was in control, resulting in reliable data.Bench Sheet: The bench sheet is used to record the most pertinentinformation about a batch of samples and helps document that the analysiswas performed correctly. In order to prevent accidental misidentificationof samples, each sample is assigned a unique ID number, along withinformation regarding sample location and the date and time the samplewas collected.Microbiological testing is time-sensitive and must be initiated within 30hours from the time of collection. Therefore, it is important to accuratelyrecord the date and time of collection and incubation so the bench sheetreflects that the samples were set up within the allowed hold time. Analysisof samples after the allowed hold time has expired can result in falsenegatives, which occur when stressed and/or dying bacteria in the sampleare not able to grow.Continued on page 2Ohio Environmental Protection AgencyDivision of Drinking and Ground WatersINSIDE THIS ISSUEThe MMO-MUG Test:Critical Documents thatMake or Break Results.1, 2Exam Info, Fall 2015 .2Online Payment Options.2Compliance-BasedReduction of DBPs inOhio .3, 4Ohio EPA’s eDocumentSearch .4Dear Answer Place .5Rule-MakingActivities 6
2015 FALL PAPER AND PENCIL EXAM DEADLINES AND DATESWastewater:Aug. 6 - Application dueNov. 4 - ExamWater:Aug. 7 - Application dueNov. 5 - ExamFor more informationCall the operator certification hotline at1-866-411-OPCT (6728) or visitepa.ohio.gov/ddagw/opcert.aspxMMO-MUG TESTContinued from page 1Incubation start and end dates and times are also recorded on the bench sheet, in order to demonstrate that thesamples were interpreted within the allowable time frame for the media being used. Colilert , Colilert-18 andColisure are three common forms of MMO-MUG media used in the laboratory. Each media has been designedso that samples are interpreted within a specific time frame, which represents the optimum time period forcoliform and E. coli growth. If a sample is incubated past the allowable time period for a particular media, falsepositives may occur. False positives can occur when background bacteria overcome inhibitors designed tosuppress growth of non-coliform bacteria.For every batch of samples analyzed and recorded on the bench sheet, positive and negative controls (or qualitycontrol samples) are also analyzed and recorded. A batch of samples is defined as no more than 60 samplesanalyzed within a four-hour period. The purpose for these controls is to demonstrate that a known negative anda known positive can produce the expected results (i.e., the negative control does not change colors while thepositive control changes colors and fluoresces under a UV light). If the controls fail to produce the expectedresults, the entire batch is considered invalid.Incubator Temperature Records: Growth of bacteria is highly dependent on the temperature at which theyare incubated. The incubator temperature record demonstrates that the incubator is being kept at an optimumtemperature for coliform growth. When using MMO-MUG media, coliform bacteria grow best when incubated at34.5 C to 35.5 C. Therefore, in addition to quality control samples, routine monitoring of the incubatortemperature is a critical aspect of analyzing for coliforms and helps ensure that the incubator has maintainedthe optimum temperature. Monitoring is done by placing a calibrated thermometer on each sample shelf andthen recording temperatures twice per day (for example, once in the morning and then once again in theafternoon at least four hours later).For questions about this article or the subject matter, please contact Ohio EPA’s Division of EnvironmentalServices (DES), Laboratory Certification Section at (614) 644-4245.ONLINE PAYMENT OPTIONSMost invoices issued by Ohio EPA may now be paid through our online eBusiness Center with a credit card or bydebiting your bank account through the Automated Clearing House (ACH). The payment options for theDDAGW program currently include fees for operator certifications and licenses to operate public water systems.To view this page, please visit https://ebiz.epa.ohio.gov. An online account is required.A service fee of 2.2% of the total amount owed will be charged for payments made by credit card.There is no service fee for ACH payments, but they do require an Ohio EPA eBusiness Center PersonalIdentification Number (PIN). If you have a PIN for another eBusiness service, that same PIN may be used to payby ACH. If you do not have a PIN, you may request one by completing the form available at the eBusinessCenter.For support, please contact Ohio EPA weekdays from 8 a.m. – 5 p.m. at (877) 372-2499.
COMPLIANCE-BASED REDUCTION OF DBPS IN OHIOThe State of Ohio completed the transition to Stage 2 Disinfection/Disinfectant Byproduct (DBP) Rule(Stage 2 Rule) from the Stage 1 DBP Rule in January 2013. Since that time, public water system (PWS)operators have worked diligently with Ohio EPA to take a multifaceted approach to reducing DBPformation. As a result, water quality in Ohio’s distribution systems has drastically improved.The operational evaluation level (OEL) is one piece of the Stage 2 Rule contributing to improved waterquality in distribution systems. The purpose of the OEL is to serve as a warning to utilities that DBP levelsare approaching an exceedance of the maximum contaminant level (MCL). Beginning with the transitionfrom the Stage 1 DBP Rule to the Stage 2 Rule in 2012, Ohio has seen 85 PWSs exceed the OEL at leastonce for either TTHM or HAA5. Of those 85 systems, 62 have exceeded the MCL for either TTHM orHAA5.Ohio PWSs are using a variety of methods to reduce DBP levels in order to comply with the Stage 2 Rule.Methods include optimization of operations through adjusting coagulant dosage, reducing prechlorination, and implementing flushing programs, including the use of automatic flush hydrants. OtherOhio utilities have intensified the fight to reduce DBPs by installing treatment, including granularactivated carbon (GAC) filters for removal of DBP precursors, passive and active mixing, as well asaeration in clear wells and storage tanks to remove formed DBPs. Many other strategies for DBPreduction are available; there is no “one-size fits all” plan, but utility operators and managers should usethe best method for their water system.Responses to elevated DBP levels may vary, but will generally fall into one of the following threecategories:1) Investigation2) Operational Optimization3) Treatment InstallationCollect special purposesamples at significantlocations to identifyproblem areas.Implement preventive tankmaintenance programs, increasemixing and reduce turnover time intanks.Remove DBP precursors at the plantthrough enhanced coagulation/softening, activated carbonadsorption, anion exchange, and/ormembrane filtration.Use on-site analyzers toreduce sample resultturnover time.Perform scheduled distributionflushing programs to reduce waterage.Remove DBPs after formationthrough tank mixing/aeration orbiological filtration.Identify the source ofhigh DBPs.Manage chlorine residuals at allpoints in distribution.Continued on page 4Ohio EPA Spigot News Summer 2015 Page 3
COMPLIANCE-BASED REDUCTION OF DBPSWhat happens if my system exceeds the OEL and/or the MCL?Continued from page 3Many PWSs initiate an investigative process in order to mitigate high DBP levels before their systemexceeds the OEL. It is especially important for purchased water systems to identify a starting point forDBP levels at the point of purchase from the wholesaler because remediation might not be worthwhile.Special purpose sampling for all systems with high DBPs will help to identify the areas of concern. Ifoperational modifications are made it is a good idea to only make one change at a time in order toidentify what type of impact the change has made.When a PWS exceeds the OEL, they will first be contacted by Ohio EPA with a request to complete anOEL report addressing water treatment, distribution and storage in relation to DBP levels. Ohio EPAstaff will be available to help identify the problem and make necessary modifications to reduce DBPs. Ifthe OEL is exceeded in future quarters, the system will be required to re-submit the report withadditional information related to any progress made since the last exceedance.If a PWS exceeds both the OEL and MCL in the same quarter, it is required to complete the OEL reportin addition to the public notice for the violation. Continued exceedance of the MCL will result ininitiation of an enforcement action by Ohio EPA. It is highly recommended that PWS operators andmanagers take a proactive approach to operational control of DBP formation beginning at the momenthigh levels are reported. The OEL and other investigative tools, shown in the table on page 3, should beused to identify the problem and ensure that necessary changes are made in an effort to provide the bestproduct to the customers and to avoid violation of the Stage 2 Rule.OHIO EPA’S eDOCUMENT SEARCHIn an effort to make public documents more easily available, Ohio EPA has created the eDocument(eDoc) Search, designed to improve efficiency; reduce costs; and greatly improve the public's ability toaccess Ohio EPA's public records from anywhere without incurring copying costs.The system currently includes the following document types, with a few exceptions.Jan. 1, 2007 - CurrentBilateral Compliance Agreement(BCA)Director’s Final Finding andOrders (DFFO)Judicial OrderRescissionNotice of Violation (NOV)Warning LetterReturn to Compliance (RTC)Ohio EPA Spigot News Summer 2015 Page 4
eDOCUMENT SEARCHContinued from page 4As of Sept. 30, 2014, all outgoing documents going forward pertaining tothe following:AdjudicationAppealAssessmentCompliance NotificationCost RecoveryCovenantDirector’s AuthorizationGeneral CorrespondenceInspection or Compliance ReviewLab CertificationLong-Term Planning for RegulatedEntityMonitoring or SamplingNon-permit Related ExemptionsNon-permit Related VarianceOhio EPA MonitoringPermit – IntermediatePermit – Long-TermPermit – Short-TermPlanReferral to Attorney GeneralRegistrationRemediation ReportRemediation ResponseReportSettlement Cost RecoveryTechnical AssistanceUIC Monitoring ReportVerified ComplaintAs of Dec. 15, 2014, most incoming documents pertaining to all of thedocument types above are being added as they are created, but there is nobacklog before that date. The only exception are those documents thatcome into the agency typically bound (large documents usually spiralbound or contained in binders.) The agency plans to add these goingforward in 2015.To get started with Ohio EPA’s eDocument Search, visit epa.ohio.gov/dir/publicrecords. This site provides frequently asked questions,search tips, a quick start video and a link to start a search.Currently, there are more than 112,000 documents loaded into eDoc andavailable for use. Ohio EPA has an estimated 8 million documents toeventually upload into the system. It is the agency’s intent to loaddocuments based on the amount the document is requested, theusefulness of the document to the public and agency staff and thedocument’s retention schedule.DEAR ANSWER PLACE:I heard we can makecredit card payments foroperator certificationonline. Is this true?- J.D.DEAR J.D.:Yes! The OperatorCertification Unit hasbeen added to the OhioEPA eBusiness Center oreBiz. Operators mayapply for examinations,submit Third Party ExamCertification applicationsand renew theircertifications online. eBizwill accept credit cardand electronic checkpayments. To learn more,click on the eBiz tab atepa.ohio.gov/ddagw/opcert.aspx.- Answer PlaceHAVE A QUESTIONFOR ANSWER PLACE?Ask a question at http://ohioepa.custhelp.comOhio EPA Spigot NewsSummer 2015Page 5
OHIO EPA’SSPIGOT NEWSJohn R. Kasich, GovernorMary Taylor, Lt. GovernorCraig W. Butler, DirectorProduced by the Division ofDrinking and Ground WatersEditors: Susan Kramer andJulie SpanglerContributing Writers:Mike Deal,Mark Tomasi,Linda Oros andJessica DingmanOhio EPA is anEqual Opportunity EmployerRULE-MAKING ACTIVITIESBelow is a brief summary of recent and upcoming rule changes. For moredetails, including notice of opportunities to comment on draft rules, signup for our electronic mailing list, or visit us on the web at epa.ohio.gov/ddagw.Recently adopted Interested Party Review (IPR) Revised Total Coliform Rule (RTCR); propose to file, tentatively Fall2015 Water Well Standards in Chapter 3745-9 and Plan Approval rules inChapter 3745-91 of the Administrative Code; second round of IPR,tentatively Summer 2015 Underground Injection Control amendments in Chapter 3745-34 of theAdministrative Code; second round of IPR, tentatively Summer 2015 Backflow Prevention & Cross-Connection Control amendments inChapter 3745-95 and additional rule amendments in Chapter 3745-91and 3745-96 of the Administrative Code; second round of IPR heldJan. 2015; propose with JCARR, tentatively Summer 201524-HOUREMERGENCY HOTLINEFor emergencies occurringafter 5 p.m. or on a weekendor holiday, please call OhioEPA’s emergency hotline at(800) 282-9378Ohio EPADivision of Drinkingand Ground WatersP.O. Box 1049Columbus, Ohio 43216-1049Phone: (614) 644-2752Fax: (614) 644-2909Visit us on the web atepa.ohio.gov/ddagwLaboratory Certification rules in Chapter 3745-89 of theAdministrative Code and Laboratory Manuals (effective May 4, 2015)In The Works Early Stakeholder Outreach (ESO) for rules covering harmful algalblooms for PWSs, June 1—June 30, 2015 ESO for rules covering Operator Certification, May 27—June 24, 2015 ESO for rules covering Secondary Drinking Water Standards andEmergency Loans, May 11—June 5, 2015 ESO for rules covering Contingency Plan Requirements for PWSs, May6—June 3, 2015 ESO for rules covering Plan Approval and Consumer ConfidenceReports, April 1—April 30, 2015 ESO for rules covering Approval Criteria for Treatment Technologiesand General Planning, March 20—April 20, 2015 Finalized 2015-2017 DDAGW Rule-Making Plan, March 9, 2015RECEIVE THE SPIGOT NEWSThe Spigot News is sent only to subscribers! Sign up to receive it inelectronic form at p/enduser/doc serve.php?2 subscriptionpage.Printed on Recycled PaperOhio EPA has a new look!
water program and how the information fits together to support accurate results. Two documents discussed in this article, the Microbiological Test Data Sheet (bench sheet) and the Incubator Temperature Records, are directly linked to the analytical process of this method. The information recorded
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