Nelson Annual Ryegrass - Texas A&M University

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Nelson Annual RyegrassL. R. Nelson and Jim Crowder„Nelson‟ is a forage-type annual ryegrass (Lolium multiflorum Lam.) developed by the TexasAgriLife Research. Nelson is a tetraploid where 2n 4x 28 chromosomes. This line has beenevaluated in forage trials at Overton, College Station and Beaumont for the past four years. Ithas also been evaluated in Mississippi, Oklahoma and Florida in official state experiment stationforage trials.Main Attributes Large plant stature compared to diploid cultivars provides high forage yield potential Good crown rust resistance Wide range in adaptability across southern USA Has wider leaves than TAM 90 and Gulf, but is similar to TAMTBODescription of LineNelson was developed for high forage production in the Deep South and Mid South of the USA.It can be over-seeded on warm season perennial pastures or drilled into prepared seedbeds in theautumn. This contrasts with TAES releases such as „Gulf‟ (Weihing, 1963) and „TAM 90‟(Nelson et al., 1992) which are diploid cultivars; however, Nelson is related to „TAMTBO‟(Nelson et al., 2007) a tetraploid cultivar released in 2006. Nelson was the second tetraploidryegrass cultivar released by Texas AgriLife Research.Plant stature (Table 1) of Nelson was 124 cm and is larger and taller than Gulf (96 cm), butsimilar to tetraploid cultivars TAMTBO and Jumbo (Prine et al., 2002) which were 125 and 128cm, respectively.Flag leaf length (Table 1) of Nelson was less (p 0.05) than Jumbo, Prine(Ball and Blount, 2002) and TAMTBO, but longer than Gulf. Nelson had a mean number of 31spikelets/spike (Table 1) compared to 37 for Jumbo and Prine, 38 for TAMTBO, and only 25 forGulf. In regard to length of 10 seed, Nelson was similar to Jumbo, Prine, and TAMTBO;however, Nelson differed from other cultivars for length of 10 seed (Table 1), where Nelson was

6 mm shorter than „Tetrapro‟ and 6 mm longer than Gulf. Nelson also had a slightly higher 1000seed weight compared to Jumbo, TAMTBO, and Gulf (Table 1).Freeze damage has notoccurred on Nelson over the past four years at Overton when a low temperature of 11 Foccurred in 2010. Nelson is not infected with the fungal endophyte. The fluorescence level ofNelson according to the Oregon State Seed Testing Laboratory is 99.49%. The ploidy level is100% tetraploid.Breeding History Of NelsonNelson is a 3-way cross from ryegrass breeding lines TXR2000-T2, TXR2002-T17, and thecultivar Jumbo (Prine et al., 2002). Jumbo is a tetraploid cultivar released by the University ofFlorida. The 3-way cross was made by growing adjacent rows (replicated) of TXR2000-T2,TXR2002-T17, and Jumbo. Any plants not exhibiting good forage potential (large plants withwide leaves and high tiller number) were removed from the population prior to cross-pollinating.Seed was harvested from individual plants and bulked. In 2002-2003, and in 2003-2004 thisseed was grown in a space planting (500 plants) at Beaumont, Texas. Best plants were selectedand produced seed each year. In 2004-2005, seed was increased in Oregon, returned to Overtonand designated TXR2006-T22, which was later named Nelson.Performance Of NelsonTexas TrialsNelson produced excellent forage yields over the past 4 years at Overton, College Station andBeaumont, Texas. Table 2 provides the ryegrass forage yields at Overton for 2009-2010 and 3year means. Nelson had a total season yield of 6381 lb/ac; however, it‟s 3-year mean yield of9556 lb/ac was second in yield. This indicates Nelson has high forage yielding potential inNortheast Texas. Table 3 provides the forage yield of Nelson in the 2009-2010, and 3-year meanin the ryegrass forage trial at College Station. Nelson produced a total season yield of 12,517lb/ac which led the trial for that year. Nelson 3-year mean (including two previous years atBeaumont) was 7160 lb/ac which was the second highest yielding cultivar over that 3-yearperiod.

RESULTS FROM FORMER YEARS: Table 4 lists the forage yields at Overton of allryegrass varieties tested in 2007-2008 as well as a 3-year mean of those varieties tested over thatperiod. Nelson had the highest numerical forage yield for the 2007-2008 growing season;however, several other varieties were not statistically different. Nelson was significantly(p 0.05) higher yielding compared to Jumbo, Prine, TAM 90, and other entries. In comparingthe 3-year mean, Nelson and TAMTBO had the two highest yields at Overton. Forage yields atBeaumont (Table 5) indicated that Nelson had the second highest forage yield for the seasontotal as well as for the three year mean. The total season yield of 5325 lb/ac was significantlyhigher than all cultivars other than Tetrapro and Big Boss. In growing season 2006-2007 (Table6) at Overton,Nelson produced an average yield compared to other entries. At Beaumont in2006-2007 (Table 7), Nelson produced the 5th highest yield which was not significantly lowerthan the top numerical forage yield. Forage yields for 2005-2006 at Overton (Table 8) indicatedNelson produced a total season yield of 7316 lb/ac and was ranked 2nd in the trial and notsignificantly different than TAMTBO. At Beaumont in 2005-2006 (Table 9), Nelson producedthe 4th highest yield indicating high forage production in Gulf Coast Region of Texas.Results from Other StatesIn 2007-2008 Nelson was tested by the Mississippi Agricultural and Forestry Experiment Stationat Newton and Starkville, Mississippi, and by the Noble Foundation at Ardmore, Oklahoma. Inboth the Starkville (Table 10) and Newton (Table 11) trials for total season forage yield, Nelsonwas in the top third of entries for each test and not significantly different from the highestyielding entry. In the Noble Foundation trial (Table 12) Nelson had a forage yield of 7420 whichwas the highest yielding entry in the experiment. These data indicate that Nelson has high forageyielding potential and good winter hardiness in Oklahoma which is in the northern region of theryegrass growing belt. In the previous year or 2006-2007 in Mississippi (Table 13) at Newton,Nelson was only average; however, there was a very high LSD value in that experiment. AtStarkville, Nelson produced an above average yield and its yield was not significantly differentfrom the top yielding entry in the trial. In the Noble Foundation trial (Table 14) Nelson did notproduce a good forage yield. Nelson was also tested at Gainesville, Florida in 2006-2007 (Table15), where it ranked 6th in the trial and was not different from the highest yielding entry. From

the data presented in these trials, it appears that Nelson has very good forage yielding potentialfor all of the southern region of the USA.Disease DataCrown rust (Puccinia coronata Corda) data are shown for Beaumont in 3 years (Tables 5, 7,and 9). In these replicated trials Nelson demonstrated good crown rust resistanceMarshall is asusceptible cultivar and it was rated fairly high or susceptible each year. In crown rust ratings ina crown rust nursery conducted by Dr. G. M. Prine at Gainesville, Florida, (a location wherecrown rust epidemics are very severe most years) shows that Nelson had relatively goodtolerance to this disease (Table 16). Nelson was similar to Jumbo, TAM 90, and TAMTBO incrown rust ratings and more resistant than Marshall. Dr. Prine also rated the ryegrass nursery forgray leaf spot (Pyricularia grisea) at Gainesville, Florida (Table 15). These data indicate thatNelson had good to average tolerance to gray leaf spot and was similar to Jumbo and TAMTBO,but more tolerant than TAM 90 or Gulf. Gray leaf spot has not been reported on ryegrass inTexas during the past 30 years.Literature CitedBall, D.M. and A.R. Blount. 2002. New (2000-2003) Forage Varieties for the Southeast. In:Proc. 57th Southern Pasture and Forage Crop Improvement Conference, Athens, GA. 4 p.Nelson, L.R., F.M. Rouquette, Jr. and G.H. Evers. 1992. Registration of „TAM 90‟ annualryegrass. Crop Sci. 32:828.Nelson, L.R., J. Crowder, F. T. Turner, G. W. Evers and F. M. Rouquette, Jr. 2007. Registrationof „TAMTBO‟ annual ryegrass. J. Plant Registrations. 1:127.Prine, G. M., A. R. Blount, L. S. Dunavin, P. Mislevy and R. L. Stanley, Jr. 2002. Registrationof „Jumbo‟ annual ryegrass. Crop Sci. 42:1749.Arnold, B. L., C. E. Watson, Jr. and N. C. Edwards, Jr. 1981. Registration of „Marshall‟ annualryegrass. Crop Sci. 21:474.Weihing, R. M. 1963. Registration of „Gulf‟ annual ryegrass. Crop Sci. 3:366.

Table 1. Plant characters of Nelson which differed from other varieties in Plant Variety ProtectionNursery at Overton, Texas in 2008.GenotypePlantFlag leaf SpikeletsLength1000 SeedHeading AnthesisHeightLengthPer spike10 seedWtDateDatecmcmNo.mmmgOf YearOf YearNelson124 a66 bc31 b60 bc3280 ab119120Tetrapro127 a78 a36 a66 a3450 a117121Jumbo128 a77 a37 a65 ab3100 b121122Prine124 a79 a37 a60 bc3290 ab113118TAMTBO125 a73 ab38 a65 ab2690 c118121Gulf96 b60 cd25 c54 d1860 d118120------------------ ----------- ----------- ----------- ----------- -------------- ----------- 37.3--lsd (0.05)4.32.83.75.2320---

Table 2. Annual ryegrass forage variety trial at Overton, TX for 2009-10 and 3-year ty1234SeasonMeanJan 27Mar 18Apr 20May 24YieldYields---------------------------lbs of dry matter/acre---------------------------RIOFlying “A”CHIPOLA2009TXR2008-T3aM/FLX2009 (4X) ERaGulfFLX2009Red 4X LateaTam 90Passeral PlusTAMTBOFLXSH2009 (2X) MEaPrineNelson07-WWaJacksonMarshallME 4aTetraproWinter Hawk07-EWaSHFL2009 (2X)aDiamond T07-MEaJumboFLX2009 (PE-2X) LRCTaME 94aMaximusB-8.1441aGo SaenSuxylPPERC2aAdrenalinPS-Lm-09-2aMeanC.V.LSD 4483798876-Experimental line, seed not commercially available.Entry not tested over last 3 years.Planted on 30 Sept. 2009. Fertilization: Pre-plant 52 lbs N, 28 lbs P2O5 and 108 lbs K2O/ac. Topdress 40 lbs N/ac on 9 Nov.2009, 26 lbs N/ac on 11 Dec. 2009, 40 lbs N/ac on 4 Mar. 2010 and 50 lbs N/ac on 29 Mar. 2010. Herbicide applied:Weedmaster at recommended rate on 9 Nov. 2009.

Table 3. Annual ryegrass forage variety trial at College Station, TX for 2009-10 and 3-yearmeans using Beaumont location data for 2007-08 and iety1234SeasonMeanJan 26Mar 12Apr 22May 25YieldYields--------------------------lbs of dry drenalin216020526118116511495-bWinter 993811435Tetrapro176818776866665111767291Diamond 15976142590101985959ME 06155TAM 4TXR2008-T3a951164560468629504Passeral Plus18691661544234093125606FLX2009Red 4X 62M/FLX2009 (4X) ERa1511234845985459002aME 9412201613543065089135524Flying 8443Go Saen1623116647427268257aFLX2009 (PE-2X) LRCT571177352735908207FLXSH2009 (2X) MEa1981198938733488191CHIPOLA2009 (2X)a2160204435603338097aSHFL2009 C.V.3216201716LSD (0.05)75339816031682190aExperimental line, seed not commercially available.Entry not tested over last 3 years.Planted on 20 Oct. 2009. Fertilizer Application: Topdressed on 13 Nov. 2009 with 50 lbs N/ac and on 22 Feb. 2010 with 40 lbsN/ac. Herbicide applied: Weedmaster at recommended rate on 13 Nov. 2009.b

Table 4. Ryegrass forage variety test at Overton, Texas for 2007-08 and 3-year riety123456Total3-YearDec 20Feb 18Mar 14Apr 7May 5May -----pounds of dry matter per 136724582166227530631273126029365Big Boss142421171893242327921132117819045Passeral ------------ ------------- ------------- ------------- ------------- ------------- ------------- ---------- 08109938935Diamond ---------------------------------- ------------- ------------- ------------- ------------- ------------- ------------- ---------- ----------Pasture Sweetner Mix1367183916562390258691810756-Flying ------------------------------------------------- ------------- ------------- ------------- ------------- ------------- ------------- ---------- ----------TAM ------------------------------- ------------- ------------- ------------- ------------- ------------- ------------- ---------- -- ------------- ------------- ------------- ------------- ------------- ------------- ---------- 261614162111LSD (0.05)4716443924675902621605Planted on 19 October 2007. Fertilizer preplant 76 lb/ac of N, 36 lb P 2O5 and 76 lb K2O/ac, respectively. Topdressed with 50 lb N/ac on 21 January, 40 lb N/ac on 20 February,and 50 lb N/ac on 9 April 2008 (as urea).Herbicide applied Weedmaster at recommended rate on 24 March 2008.

Table 5. Ryegrass forage variety test at Beaumont, Texas for 2007-08 and 3-year mean iety1234TotalMeansDec 18Jan 8Feb 28Apr 29----------------------------------pounds of dry matter per 39260956061Nelson466718343379853255819Big ----------------------------- -------------- -------------- -------------- -------------- ------------ 9342367944235178Flying ------------------- -------------- -------------- -------------- -------------- ------------ -------------TAM 90201597467303042965037Diamond Passeral --- -------------- -------------- -------------- -------------- ------------ - -------------- -------------- -------------- -------------- ------------ e Sweetner ---------------------- -------------- -------------- -------------- ------------- ------------ -LSD (0.05)260261213288564--Crown 2.31.3-------------------1.111.22.1Experiment planted on 4 October 2007. Fertilizer application was 48 lb/ac of N, P 205 and K20 on 10 October 2007. Topdressed with 108 lb N/ac on 18 December and 36 lb N/acon 13 March 2008.bCrown rust ratings were recorded on 13 March 2008 and were on a 0-9 scale where 0 no disease and 9 most severe.

Table 6. Ryegrass forage variety test at Overton, Texas for TotalVariety123456DMY12 Dec7 Feb6 Mar23 Mar19 Apr14 May-------------------------------------pounds of dry matter per 4846814721578294912678782Surrey --- ------------- ------------- ------------- ------------- ------------- ------------- 49Dynagain10417061544150627939488538Passerel ----- ------------- ------------- ------------- ------------- ------------- ------------- ---------WD-40146410621431129525016988451Big ----- ------------- ------------- ------------- ------------- ------------- ------------- ---------Flying 37953TAM 31120357597861Diamond - ------------- ------------- ------------- ------------- ------------- ------------- 986731171129923729227135------------------------- ------------- ------------- ------------- ------------- ------------- ------------- 111710LSD (0.05)6384343033624332421210Planted on 21 September 2006. Fertilization: Preplant 60 lb N, 20 lb P2O5 and 120 lb K2O/ac. Topdressed with 50 lb N on 3October; 52 lb N on 11 January, 50 lb N on 12 February and 40 lb N on 23 March as ammonium nitrate.*Means include yields of several experimental lines not shown.Herbicide application: Applied Weedmaster at recommended rate on 5 October 2006.

Table 7. Ryegrass forage yields at Beaumont, Texas for nVariety12345Total12 Dec9 Jan1 Feb2 Mar10 unds dry ------Tetrapro66810191172137121746404Diamond 848Big ------------Flying A7391041983102114825265TAM 385199Shwasuaoba8371733106811413865164Surrey 4105818834929Passerel ---------------------------------------Mean **619879966108316675215LSD P 0.05272365210221324491Planting date: 4 October. Fertilization: Applied 38 lb/ac of N, P 2O5 and K2O on 25 October. Topdressed: 98 lb N/ac on 11 January 2007.Crown RustRating ----

*Crown rust ratings recorded on 9 April on a 0 to 3 scale where 0 no rust and 3 20% leaf area rusted.**Means include yields for several experimental lines not shown.Data collected by Mike Jund, TAMU at Beaumont.

Table 8. Total ryegrass forage yields at Overton, Texas for 12345Total26 Jan8 Mar30 Mar26 Apr24 ---------------pounds dry 27165Jumbo109513211111243611897152TAM 7025Big 67Maximus10931502116521238116694Passerel 5279496087Surrey II10311389111715349676038Diamond 6106098918438985106Flying ean*9671360121718258946264

cvLSD (0.10)383351721313147152423024211647Planted 3 October 2005. Preplant fertilizer: 60 lb N, 80 lb P 2O5 and 0 lb K2O per acre. Topdressed with 40 lb N/ac on 11 November 2005, 20 lb N/ac on 31 January 2006, 20 lb N/ac on 1 March and45 lb N/ac on 22 March 2006, respectively.*Means shown include yields from several experimental lines not shown.

Table 9. Total ryegrass forage yields at Beaumont, Texas for 2005-2006.HarvestHarvestHarvestHarvestHarvestCrown RustVariety12345SeasonRating12 Dec9 Jan1 Feb2 Mar10 ---------------------------pounds dry 281.0aDiamond 103914108151039177160740.7------------------- ------------------- ------------------- ------------------- ------------------- ------------------- ------------------- Dynagain117713427521039167059801.0Big ----------- ------------------- ------------------- ------------------- ------------------- ------------------- ------------------- 0TAM 9080112287811197158955960.7Flying ---- ------------------- ------------------- ------------------- ------------------- ------------------- ------------------- N 948124846980.7------------------- ------------------- ------------------- ------------------- ------------------- ------------------- ------------------- al Plus1014914461835110443290.7Surrey 302.0------------------- ------------------- ------------------- ------------------- ------------------- ------------------- ------------------- ------------------Mean *8601132684107714335164LSD P 0.05311242230186494Planted October 13, 2005. Fertilization was 45 lb of N, P 2O5 and K2O per acre on November 10, 2005. Topdressed with 64 lb/ac on December 12, 2005 and 56 lb/ac on February 1, 2006.

aCrown rust ratings recorded on April 6, 2006. Values are the average of 3 replications.*Means include yields of several experimental lines not shown.

Table 10. Annual ryegrass at Starkville, Mississippi ty1234520 Nov15 Jan6 Mar2 Apr6 May---------------------------------pounds dry 149424834419Big ---------------------------Diamond d (L5-1-AR102)13441945143420413289FL/NE2006 (misc2x)LRCT8692043177419593540FLX 2002 (LA 3) ----Flying 2620443501Passerel ----Pasture Sweet‟ner Mix5301800121321563208Prine 310TAM 3746Harvest611 32

Tetrastar -----------LSD (P 044---------26820.6928Planted: September 27, 2008. Soil: Savannah sandy loam. Fertilizer: 400 lb/ac 15-5-10 at planting and after each harvest. Herbicide: glyphosate at 1 qt/ac 2 weeks prior to ---12307.711344

Table 11. Annual ryegrass at Newton, Mississippi, 7 Dec18 Feb3 Apr14 May---------------------------pounds dry g -------------------------------Diamond T2337227327442544DynaGain2607215930322302Ed 921240330142625FLX 2002 (LA 3) ------------Flying 42618268828632409ME942516284129442248Passeral ------------Pasture Sweet‟ner Mix1655200528842434Prine Tetraploid2366221229242689Rio2398246530232505TAM 110391962510193----------9869

Tetrastar TetraploidTXR 2006 ------------LSD (P 32568------------48812.52392Planted: September 28, 2008. Soil: Prentiss Fine Sandy Loam. Fertilizer: 500 lb/ac 13-13-13 onOctober 16, 2008 and 2000 lb/ac following each harvest. Herbicide: 031810526----------8935.410047

Table 12. Dry matter forage yields of a

Nelson according to the Oregon State Seed Testing Laboratory is 99.49%. The ploidy level is 100% tetraploid. Breeding History Of Nelson Nelson is a 3-way cross from ryegrass breeding lines TXR2000-T2, TXR2002-T17, and the cultivar Jumbo (Prine et al., 2002). Jumbo is a tetraploid cultivar released by the University of Florida.

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