Digital Wine Refractometers

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INSTRUCTION MANUALMA882, MA883,MA884, MA885Digital WineRefractometersSATISFACTIONGUARANTEED

THANK YOU for choosing Milwaukee Instruments!This instruction manual will provide you the necessaryinformation for correct use of the meters.All rights are reserved. Reproduction in whole or in part isprohibited without the written consent of the copyright owner,Milwaukee Instruments Inc., Rocky Mount, NC 27804 USA.

3TABLE OF CONTENTS1. FUNCTIONAL DESCRIPTION.52. GENERAL DESCRIPTION.83. SPECIFICATIONS.94. PRINCIPLE OF OPERATION. 105. MEASUREMENT UNITS.116. MEASUREMENT GUIDELINES.137. CALIBRATION PROCEDURE. 148. MEASUREMENT PROCEDURE. 169. CHANGING MEASUREMENT UNIT (MA884 & MA885). 1810. CHANGING TEMPERATURE UNIT. 2011. CHANGING POTENTIAL ALCOHOL CONVERSION FACTOR(MA884 only).2112. MAKING A STANDARD %BRIX SOLUTION.2213. ERROR MESSAGES.2314. BATTERY REPLACEMENT. 25CERTIFICATION. 26RECOMMENDATION. 26WARRANTY. 26

4 MA882, MA883, MA884, MA885 Digital Wine RefractometersRemove the instrument from the packing materials and examinecarefully to ensure no damage has occurred during shipping. Ifany damage has occurred, notify your Dealer.Each instrument is supplied with:y 9 V batteryy Instruction manualNote: Save all packing material until you are sure that theinstrument functions correctly. A defective instrument must bereturned in its original packing.

FUNCTIONAL DESCRIPTION 51. FUNCTIONAL DESCRIPTIONDISPLAYA. BATTERY STATUS ICON (BLINKS WHEN LOW BATTERYCONDITION DETECTED)B. MEASUREMENT IN PROGRESS TAGC. SETUP: FACTORY CALIBRATION TAGD. CAL: CALIBRATION TAGE. AUTOMATIC TEMPERATURE COMPENSATION (BLINKS WHENTEMPERATURE EXCEEDS 10-40 C / 50-104 F RANGE)F. PRIMARY DISPLAY (DISPLAYS MEASUREMENT AND ERRORMESSAGES)G. TEMPERATURE UNITSH. SECONDARY DISPLAY (DISPLAYS TEMPERATUREMEASUREMENTS; WHEN BLINKING, TEMPERATURE HASEXCEEDED OPERATION RANGE: 0-80 C / 32-176 F)I. RANGE INDICATOR (MA884 and MA885)

6 MA882, MA883, MA884, MA885 Digital Wine RefractometersFRONT PANELAHGFMA882Digital Wine Refractometer0 to 50 %BrixBCAEHGFMA885Digital WineRefractometer1 %Brix2 Oechsle ( Oe)3 KMW ( Babo)BA.B.C.D.E.F.G.H.CDELIQUID CRYSTAL DISPLAY (LCD)READ KEY (USER MEASUREMENT)ZERO KEY (USER CALIBRATION)RANGE KEY (MA884 and MA885)ON/OFFSTAINLESS STEEL SAMPLE WELL AND PRISMSECONDARY DISPLAYPRIMARY DISPLAY

FUNCTIONAL DESCRIPTION 7BOTTOMIJI. BATTERY COVERJ. BATTERY COMPARTMENT

8 MA882, MA883, MA884, MA885 Digital Wine Refractometers2. GENERAL DESCRIPTIONSIGNIFICANCE OF USEThank you for choosing Milwaukee. This instruction manual willprovide you the necessary information for correct use of the meter.The MA882, MA883, MA884 and MA885 are optical instrumentsthat are based on the measurement of the refractive index of asolution. The measurement of refractive index is simple andquick and provides the vintner an accepted method for sugarcontent analysis. Samples are measured after a simple usercalibration with deionized or distilled water. Within secondsthe instrument measures the refractive index of the grape. Thisdigital refractometers eliminate the uncertainty associatedwith mechanical refractometers and are easily portable formeasurements in the field.The four instruments utilize internationally recognized referencesfor unit conversion and temperature compensation.MA882measures%BrixMA883measures BauméMA884measures%BrixPotential Alcohol (% vol)MA885measures%Brix Oechsle ( Oe) KMW ( Babo)Temperature (in C or F) is displayed simultaneously with themeasurement on the large dual level display along with icons forLow Power and other helpful message codes.Key features include:y Dual-level LCDy Automatic Temperature Compensation (ATC)y Easy setup and storagey Battery operation with Low Power indicatory Automatically turns off after 3 minutes of non-usey Single-point calibration with distilled or deionized watery Waterproof models offers IP65 waterproof protectiony Quick, precise results readings are displayed in approximately1.5 secondsy Small sample size as small as 2 metric drops.

SPECIFICATIONS 93. SPECIFICATIONSMA882Range:0 to 50 %BrixResolution:0.1%Brix0.1 C (0.1 F)Accuracy: 0.2%Brix 0.3 C ( 0.5 F)MA883Range:Resolution:Accuracy:MA884Range:0 to 28 Baumé0 to 80 C (32 to 176 F)0 to 80 C (32 to 176 F)0.1 Baumé0.1 C (0.1 F) 0.1 Baumé 0.3 C ( 0.5 F)0 to 500 to 25Resolution: 0.10.1Accuracy: 0.2 0.2MA885Range:0 to 500 to 2300 to 42Resolution: 0.110.1Accuracy: 0.2 1 0.2%Brix0 to 80 C (32 to 176 F)% v/v Potential Alcohol%Brix0.1 C (0.1 F)%v/v Potential Alcohol%Brix 0.3 C ( 0.5 F)% v/v Potential Alcohol%Brix Oechsle KMW%Brix Oechsle KMW%Brix Oechsle KMW0 to 80 C (32 to 176 F)0.1 C (0.1 F) 0.3 C ( 0.5 F)Common SpecificationsLight SourceMeasurement TimeMinimum Sample VolumeSample CellTemperatureCompensationCase MaterialEnclosure RatingBattery Type/LifeAuto-Shut offDimensionsWeightYellow LEDApproximately 1.5 seconds100 µL (cover prism totally)Stainless Steel ring and flint glass prismAutomatic between 10 and 40 C (32 to 104 F)ABSIP 651 x 9 volt AA batteries / 5000 readingsAfter 3 minutes of non-use19.2 x 10.2 x 6.7 cm (7.5 x 4 x 2.6")420 g (14.8 oz.).

10 MA882, MA883, MA884, MA885 Digital Wine Refractometers4. PRINCIPLE OF OPERATION%Brix, Baumé, Oechsle, KMW and % potential alcoholdeterminations are made by measuring the refractive indexof a solution. Refractive Index is an optical characteristic of asubstance and the number of dissolved particles in it. RefractiveIndex is defined as the ratio of the speed of light in empty spaceto the speed of light in the substance. A result of this property isthat light will “bend”, or change direction, when it travels througha substance of different refractive index. This is called refraction.When passing from a material with a higher to lower refractiveindex, there is a critical angle at which an incoming beam oflight can no longer refract, but will instead be reflected off theinterface. The critical angle can be used to easily calculate therefractive index according to the equation:sin (θcritical) n2 / n1Where n2 is the refractive index of the lower-density medium; n1 isthe refractive index of the higher-density medium.θrθrn2θin1n2θin2n1θiCritical Angleθ i θ criticalRefractionθ i θ criticaln1Total Internal Reflectionθ i θ criticalθ i angle of incidenceθ r angle of refractionn1 , n2 - refractive indexIn the MA882, MA883, MA884 and MA885 refractometers, lightfrom an LED passes through a prism in contact with the sample.An image sensor determines the critical angle at which the light isno longer refracted through the sample.Sample WellSampleLedPrismLensShadowLinear Image SensorSpecialized algorithms then apply temperature compensation tothe measurement and convert this refractive index to the modelspecific measurement unit.

MEASUREMENT UNITS 115. MEASUREMENT UNITSMA882, MA883, MA884 and MA885 measure sugar content inseveral units to meet the differing requirements found in thewine industry.MA882, MA884 and MA885 convert the refractive index of thesample to sucrose concentration in units of percent by weight,%Brix (also referred to as Brix). The conversion used is based onthe ICUMSA Methods Book (International Commission for UniformMethods of Sugar Analysis). Since the majority of sugar in grapejuice is fructose and glucose and not sucrose, the reading issometimes referred to as “Apparent Brix”.MA883 has units of Baumé. The Baumé scale is based on densityand was originally designed to measure the mass of sodiumchloride in water. Baumé is used in wine making to measure thesugar in must. The MA883 converts the %Brix reading to Baumébased on the table found in the Official Methods of Analysis ofAOAC International, 18Th Edition. 1 Baumé is approximately equalto 1.8 %Brix, and 1 Baumé is roughly equivalent to 1 % alcoholwhen the wine is fully fermented.In addition to %Brix, MA885 includes two other scales used in thewine industry: Oechsle and KMW. Oechsle ( Oe) is mainly used in the German, Swiss andLuxenburgish winemaking industry to measure the sugar contentof must. The Oe scale is based on specific gravity at 20 C(SG20/20) and is the first 3 digits following the decimal point. 1 Oeis roughly equal to 0.2 %Brix. Oe [(SG20/20) - 1] x 1000 Klosterneuburger Mostwaage ( KMW) is used in Austria tomeasure the sugar content of must. KMW is related to Oe by thefollowing equation: Oe KMW x [(0.022 x KMW) 4.54]1 KMW is roughly equivalent to 1 %Brix or 5 Oe. KMW is alsoknown as Babo.In addition to %Brix, MA884 also has a second scale that estimatesthe alcohol content in the finished wine in (% vol/vol). This isknown as “potential” or “probable“ alcohol since the conversion

12 MA882, MA883, MA884, MA885 Digital Wine Refractometersbetween sugar and alcohol depends on many factors such as thetype of grapes, the grape maturity, the growing region and yeastfermentation efficiency and temperature.Since no fixed conversion factor is universally applicable, MA884allows the user to taylor the instrument to their specific needsbased on their experience.The first conversion is based on the %Brix value, with anadjustable conversion factor anywhere between 0.50 and 0.70(0.55 is a common value).Potential alcohol (% v/v) (0.50 to 0.70) x %BrixOne drawback of the above equation is that it does not take intoaccount the nonfermentable sugars and extract.A second equation was also added that takes these factors intoaccount and can give a more accurate estimate of the alcoholcontent in the finished wine. This conversion is named “C1” on themeter, and uses the following equation:Potential Alcohol (% v/v) 0.059 x [(2.66 x Oe) - 30](C1)

MEASUREMENT GUIDELINES 136. MEASUREMENT GUIDELINESy Handle instrument carefully. Do not drop.y Do not immerse instrument under water.y Do not spray water to any part of instrument except the“sample well” located over the prism.y The instrument is intended to measure grape/wine solutions.Do not expose instrument or prism to solvents that willdamage it. This includes most organic solvents and extremelyhot or cold solutions.y Particulate matter in a sample may scratch the prism. Absorbsample with a soft tissue and rinse sample well with deionizedor distilled water between samples.y Use plastic pipettes to transfer all solutions. Do not usemetallic tools such as needles, spoons or tweezers as thesewill scratch the prism.y Cover sample well with hand if measuring in direct sun.

14 MA882, MA883, MA884, MA885 Digital Wine Refractometers7. CALIBRATION PROCEDURECalibration should be performed daily, before measurements aremade, when the battery has been replaced, between a long seriesof measurements, or if environmental changes have occuredsince the last calibration.1. Press the ON/OFF key, then release. Two instrument testscreens will be displayed briefly; all LCD segments followedby the percentage of remaining battery life. When the LCDdisplays dashes, the instrument is ready.2. Using a plastic pipette, fill the sample well with distilled ordeionized water. Make sure the prism is completely covered.Note: If the ZERO sample is subject to intense light such assunlight or another strong source, cover the sample well withyour hand or other shade during the calibration.3. Press the ZERO key. If no error messages appear, your unit iscalibrated. (For a description of error messages see ERRORMESSAGES section).

CALIBRATION PROCEDURE 15Note: The 0.0 screen will remain until a sample is measured orthe instrument is turned off.4. Gently absorb the ZERO water standard with a soft tissue.Use care not to scratch the prism surface. Dry the surfacecompletely. The instrument is ready for sample measurement.Note: If instrument is turned off the calibration will not be lost.

16 MA882, MA883, MA884, MA885 Digital Wine Refractometers8. MEASUREMENT PROCEDUREVerify the instrument has been calibrated before takingmeasurements.For MA884 and MA885 select the desired measurement unit (seeCHANGING MEASUREMENT UNIT (MA884 & MA885) section).1. Wipe off prism surface located at the bottom of the samplewell. Make sure the prism and sample well are completely dry.2. Using a plastic pipette, drip sample onto the prism surface. Fillthe well completely.Note: If the temperature of the sample differs significantly fromthe temperature of the instrument, wait approximately 1 minuteto allow thermal equilibration.3. Press the READ key. The results are displayed in unit ofinterest.Note: The last measurement value will be displayed untilthe next sample is measured or the instrument is turned off.Temperature will be continuously updated.

MEASUREMENT PROCEDURE17Note: The “ATC” tag blinks and automatic temperaturecompensation is disabled if the temperature exceeds the0-40 C / 32-104 F range.4. Remove sample from the sample well by absorbing on asoft tissue.5. Using a plastic pipette, rinse prism and sample well withdistilled or deionized water. Wipe dry. The instrument is readyfor the next sample.

18 MA882, MA883, MA884, MA885 Digital Wine Refractometers9. CHANGING MEASUREMENT UNIT (MA884 & MA885)For MA884 only:1. Press the RANGE key to select measurement units of %Brixor % potential alcohol. The instrument toggles between thetwo measurement scales each time the key is pressed andthe primary display indicates “bri” for %Brix or “P.ALc” forpotential alcohol. When the instrument displays the screenwith 4 dashes, the instrument is ready for measurement.A number on the display indicates the selected unit: “1”denotes %Brix and “2” denotes potential alcohol as indicatedon the instrument cover.2. The potential alcohol scale also indicates the conversionfactor chosen for the potential alcohol reading. See CHANGINGPOTENTIAL ALCOHOL CONVERSION FACTOR to change thecurrent factor.Note: When changing ranges the presently configuredconversion factor will be displayed in the lower display. (SeeFUNCTIONAL DESCRIPTION)For MA885 only:1. Press the RANGE key to select measurement units %Brix, Oechsle ( Oe) or KMW ( Babo). The instrument togglesbetween the three scales each time the key is pressed andthe primary display indicates “bri” for %Brix, “OE” for Oechsleor “bAbo” for KMW. When the instrument displays the screenwith 4 dashes the instrument is ready for measurement. Anumber on the display indicates the selected unit: “1” denotes

CHANGING MEASUREMENT UNIT (MA884 & MA885)19%Brix, “2” denotes Oe and “3” denotes KMW as indicated onthe instrument cover.

20 MA882, MA883, MA884, MA885 Digital Wine Refractometers10. CHANGING TEMPERATURE UNITTo change the temperature measurement unit from Celsius toFahrenheit (or vice versa), follow this procedure.1. Press and hold the ON/OFF key continuously for approximately8 seconds. The LCD will display the “all segment” screenfollowed by a screen with the model number on the primarydisplay and the version number on the secondary display.Continue pressing the ON/OFF key.8 seconds2. While continuing to hold the ON/OFF key, press the ZERO key.The temperature unit will change from C to F or vice versa. C or F

CHANGING POTENTIAL ALCOHOL CONVERSION FACTOR 2111. CHANGING POTENTIAL ALCOHOL CONVERSIONFACTOR (MA884 only)To change the potential alcohol conversion factor, follow thisprocedure.1. Press and hold ON/OFF key continuously for approximately8 seconds. The LCD will display all segments screen followedby a screen with the model number on the primary displayand the version number on the secondary display. Continuepressing the ON/OFF key.8 seconds2. While continuing to hold ON/OFF, press the RANGE key.The LCD will show the current conversion factor on theprimary display and “P.ALc” on the secondary display.Continue holding the ON/OFF key. Press the ZERO key toincrease this number. The number will continually increaseuntil “0.70” is reached then wrap back to “C1”. The userselectable conversion range is 0.50 to 0.70. C1 stands for“curve 1” (see MEASUREMENT UNITS). When you reach theconversion factor you desire release the ON/OFF key. Thenew conversion factor will be used.

22 MA882, MA883, MA884, MA885 Digital Wine Refractometers12. MAKING A STANDARD %BRIX SOLUTIONy To make a Brix Solution, follow the procedure below:y Place container (such as a glass vial or dropper bottle that hasa cover) on an analytical balance.y Tare the balance.y To make an X BRIX solution weigh out X grams of high puritySucrose (CAS #: 57-50-1) directly into the container.y Add distilled or deionized water to the container so the totalweight of the solution is 100 g.Note: Solutions above 60 %Brix need to be vigorously stirredor shaken and heated in a water bath. Remove solution frombath when sucrose has dissolved. The total quantity can bescaled proportionally for smaller containers but accuracy maybe sacrificed.Example with 25 %Brix:%Brix25g Sucrose25.000g Water75.000g Total100.000

ERROR MESSAGES2313. ERROR MESSAGESError CodeDescriptionErrGeneral failure. Cyclepower to instrument. Ifinstrument still has error,contact Milwaukee.LO Primary displaySample is reading lowerthan the 0 % standardused for meter calibration.HI Primary displaySample exceeds maximummeasurement range.LO Primary displayCAL segment ONWrong calibration usedto zero instrument. Usedeionized or distilledwater. Press ZERO.HI Primary displayCAL segment ONWrong calibration usedto zero instrument. Usedeionized or distilledwater. Press ZERO.t LO Primary displayCAL segment ONTemperature exceeds ATClow limit (10 C) duringcalibration.t HI PrimarydisplayCAL segment ONTemperature exceeds ATChigh limit (40 C) duringcalibration.AirPrism surface insufficientlycovered.ELtToo much external lightfor measurement. Coversample well with hand.nLtLED light is not detected.Contact Milwaukee.

24 MA882, MA883, MA884, MA885 Digital Wine RefractometersBattery segmentblinking 5% of battery life isremaining.Temperature valuesare blinking 0.0 Cor 80.0 CTemperature measurementout of sampling range(0.0 to 80.0 C).ATC segmentblinkingOutside temperaturecompensation range (10 to40 C).SETUP segmentblinkingFactory calibration lost.Contact Milwaukee.

BATTERY REPLACEMENT 2514. BATTERY REPLACEMENTTo replace the instrument’s battery, follow these steps:y Turn the instrument OFF by pressing the ON/OFF key.y Turn instrument upside down and remove the battery coverby turning it counterclockwise.y Extract the battery from its location.y Replace with fresh 9V battery making certain to observepolarity.y Insert the back battery cover and fasten it by turningclockwise to engage.

26 MA882, MA883, MA884, MA885 Digital Wine Ref

the ICUMSA Methods Book (International Commission for Uniform Methods of Sugar Analysis). Since the majority of sugar in grape juice is fructose and glucose and not sucrose, the reading is sometimes referred to as “Apparent Brix”. MA883 has units of Baumé. The Baumé scale is based on density

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