Lab 20 – Special Senses Special Senses

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Lab 20 – Special SensesI.II.III.IUSM – 2016IntroductionSpecial SensesKeywordsSlidesA. Eye1. General structure2. Lens3. Layersa. Fibrous tunici. Corneaii. Sclerab. Vascular tunici. Irisii. Ciliary bodyiii. Choroidc. Neural tunic (Retina)4. Optic NerveB. EyelidC. Ear1. General structure2. PartsIV.a. External earb. Inner earSummaryFig 23-1, Junqueira, 13th ed.

Lab 20 – Special SensesI.II.III.IUSM – 2016IntroductionKeywordsSlidesA. Eye1. General structure2. Lens3. Layersa. Fibrous tunici. Corneaii. Sclerab. Vascular tunici. Irisii. Ciliary bodyiii. Choroidc. Neural tunic (Retina)4. Optic NerveB. EyelidC. Ear1. General structure2. PartsIV.Keywordsa. External earb. Inner earSummaryAnterior chamberBowman’s membraneCanal of SchlemmChoroidCiliary bodyCiliary processesCochleaCochlear ductConesConjunctivaCorneaDescement’s membraneDilator pupillae m.FoveaHair cellsInner limiting membraneInner plexiform layerIrisLensLimbusMeibomian glandsNucleated fibersOptic discOptic nerveOuter plexiform layerPigmented epitheliumPosterior chamberRetinaRodsScala mediaScala tympaniScala vestibuliScleral venous sinusSphincter pupillae m.Spiral ganglionSpiral organ of CortiStria vascularisSubstantia propriaSuspensory ligamentTarsal plateTectorial membraneVestibular membrane

Lab 20 – Special SensesI.II.III.Slide 78: Eye, H&EIUSM – 2016Slide OverviewIntroductionKeywordsSlidesA. Eye1. General structure2. Lens3. Layersa. Fibrous tunici. Corneaii. Sclerab. Vascular tunicanteriorcavitylenscornea(vitreous chamber)i. Irisii. Ciliary bodyiii. Choroidc. Neural tunic (Retina)4. Optic NerveB. EyelidC. Earposterior cavityoptic nerve(CN II)irisw/ pupil1. General structure2. PartsIV.a. External earb. Inner earSummaryneural tunicvascular tunicfibrous tunicsclera

Lab 20 – Special SensesI.II.III.Slide 78: Eye, H&EIUSM – 2016IntroductionKeywordsSlidesA. Eye1. General structure2. Lens3. Layersa. Fibrous tunici. Corneaii. Sclerab. Vascular tuniclensi. Irisii. Ciliary bodyiii. Choroidc. Neural tunic (Retina)4. Optic NerveB. EyelidC. Ear1. General structure2. PartsIV.a. External earb. Inner earSummaryanteriorcavityanteriorchamberbetween cornea and irisposteriorchamberbetween iris and lensposteriorcavity(vitreous chamber)the eye is divided into two cavities based upon the lens: posterior to the lens is the posterior cavity containingthe vitreous body (humor) which is a large gelatinous mass of transparent connective tissue composed mainlyof water; anterior to the lens is the anterior cavity which is subdivided into the anterior and posteriorchambers based upon the iris; the anterior cavity is filled with aqueous humor which is produced by theciliary processes in the posterior chamber and flows through the pupil into the anterior chamber

Lab 20 – Special SensesI.II.III.IUSM – 2016IntroductionKeywordsSlidesA. Eye1. General structure2. Lens3. Layersa. Fibrous tunici. Corneaii. Sclerab. Vascular tunici. Irisii. Ciliary bodyiii. Choroidc. Neural tunic (Retina)4. Optic NerveB. EyelidC. Ear1. General structure2. PartsIV.a. External earb. Inner earSummarySlide 78: Eye, H&Enon-nucleatedfibers(contain crystallin proteins)differentiationnucleated fiberssubcapsular epitheliumcapsule(basal lamina)the lens is an avascular, biconvex, transparent disc derived from epithelium and attached to the ciliary body bythe suspensory ligament; the flexibility of the lens allows it to change shape to affect light refraction upon theretina; its transparency is principally due to the loss of light-scattering organelles (such as the nucleus), thepresence of crystallin proteins, and the precise shape and organized packing of its non-nucleated fibers

Lab 20 – Special SensesI.II.III.IUSM – 2016IntroductionKeywordsSlidesA. Eye1. General structure2. Lens3. Layersa. Fibrous tunici. CorneaSlide 78: Eye, H&Ethe fibrous tunic is the outermost layerof the eye; it consists of two majorregions:1.2.ii. Sclerab. Vascular tunici. Irisii. Ciliary bodyiii. Choroidsclera - posterior 5/6th; the “white”of the eye; consists of dense CT thatprotects the eye and serves as sitefor extraocular muscle attachmentcornea - anterior 1/6th; transparentand avascular; serves as primarysite of light refraction; density ofpain receptors is 100x greater thanin skin and 10x than in dental pulpc. Neural tunic (Retina)4. Optic NervescleraB. EyelidC. Ear1. General structure2. PartsIV.a. External earlimbuscorneab. Inner earSummarycorneaconjunctivathe sclera and cornea are joined at the limbus (Lt. “edge”)which encircles the cornea and serves as its source ofstem cells; it becomes more stratified at the conjunctivawhich is the stratified columnar mucous membrane withnumerous goblet cells that covers the exposed portion ofthe sclera (not the cornea) and the inside of the eyelid

Lab 20 – Special SensesI.II.III.Slide 78: Eye, H&EIUSM – 2016IntroductionKeywordsSlidesA. Eye1. General structure2. Lens3. Layersa. Fibrous tunici. Corneaii. Sclerab. Vascular tunici. Irisii. Ciliary bodyiii. Choroidc. Neural tunic e of collagen bundles withinterspersed keratocytescornealendotheliumsimplesquamous4. Optic NerveB. EyelidC. Ear1. General structure2. PartsIV.a. External earb. Inner earSummaryBowman’s membrane(anterior limiting membrane)Descemet’s membrane (des-suh-mays)(posterior limiting membrane)

Lab 20 – Special SensesI.II.III.Slide 78: Eye, H&EIUSM – 2016IntroductionKeywordsSlidesA. Eye1. General structure2. Lens3. Layersa. Fibrous tunici. Corneaii. Sclerab. Vascular tuniccornealepitheliumi. Irisii. Ciliary bodyiii. Choroidc. Neural tunic (Retina)4. Optic NerveB. EyelidBowman’smembraneC. Ear1. General structure2. PartsIV.a. External earb. Inner earSummarythe corneal epithelium is nonkeratinized stratified squamous epithelium generally with 5-7 cell layers; cellturnover is about 7 days from stem cells located in the limbus; unlike the overlying epithelium, Bowman’smembrane is relatively static and does not regenerate after injury; the uniform orientation of the CT fiberswithin the substantia propria contributes to corneal transparency

Lab 20 – Special SensesI.II.III.IUSM – 2016IntroductionKeywordsSlidesSlide 78: Eye, H&EA. Eye1. General structure2. Lens3. Layersa. Fibrous tunici. Corneaii. Sclerab. Vascular tunici. IrisDescemet’s membrane(des-suh-mays)corneal endotheliumkeratocyteii. Ciliary bodyiii. Choroidc. Neural tunic (Retina)4. Optic NerveB. EyelidC. Ear1. General structure2. PartsIV.a. External earb. Inner earSummarythe cornea is avascular, so most metabolic exchange occurs across the corneal endothelium with the aqueoushumor in the anterior chamber; additionally the endothelium keeps the cornea dehydrated by actively pumpingNa into the chamber (water follows); Descemet’s membrane separates the endothelium from the substantiapropria, but unlike Bowman’s membrane it is readily regenerated after injury if the endothelium is intact; unlikethe corneal epithelium, the endothelium has limited regenerative potential

Lab 20 – Special SensesI.II.III.IUSM – 2016IntroductionKeywordsSlidesA. Eye1. General structure2. Lens3. Layersa. Fibrous tunici. CorneaSlide 78: Eye, H&Eretinachoroidii. Sclerab. Vascular tunici. Irisii. Ciliary bodyscleraiii. Choroidc. Neural tunic (Retina)4. Optic NerveB. EyelidC. Ear1. General structure2. PartsIV.a. External earb. Inner earSummarythe sclera (Gr. “hard”) is the opaque layer of dense CT constituting the posterior 5/6th of the fibrous tunic; itserves to maintain the rigidity of the eye and as the site of attachment for the tendons of the extraocular muscles

Lab 20 – Special SensesI.II.III.Slide 78: Eye, H&EIUSM – 2016IntroductionKeywordsSlidesA. Eye1. General structurethe vascular tunic (uvea) is the middle layer of theeye; it consists of three parts:1.2. Lens3. Layersa. Fibrous tunici. Corneaa.ii. Sclerab. Vascular tunici. Irisii. Ciliary body2.iii. Choroidc. Neural tunic (Retina)4. Optic NerveB. EyelidC. Ear1. General structure2. PartsIV.a. External earb. Inner earSummaryiris: most anterior portion of vascular tunic;contains many melanocytes that provide thecolor of the eye and prevent the passage oflight, leaving only the central pupil (opening)for light to pass through; contains:3.b.dilator pupillae m.sphincter pupillae m.ciliary body: expansion of the vascular tunic,encircling the lens; consists of:a.ciliary musclea.loose CTb.ciliary processeschoroid: located in posterior 2/3rd of eye;contains:b.c.lots of vasculaturemelanocytesciliary choroidbodyirispupil

Lab 20 – Special SensesI.II.III.Slide 78: Eye, H&EIUSM – 2016IntroductionKeywordsSlidesA. Eye1. General structure2. Lens3. Layersa. Fibrous tunici. Corneastromadilator pupillae m.ii. Sclerab. Vascular tunici. Irisii. Ciliary bodyiii. Choroidc. Neural tunic (Retina)4. Optic NerveB. Eyelidsphincter pupillae m.anteriorchamberpigmented epitheliumC. Ear1. General structure2. PartsIV.a. External earb. Inner earSummarythe iris consists of a CT stroma with an uncovered anterior surface, not lined by an epithelium bordering theanterior chamber; the posterior pigmented epithelium blocks light entering the eye except through the pupiland separates the stroma from the posterior chamber, however the basal lamina faces the chamber side, not thestroma; the sphincter pupillae m. has parasympathetic innervation and constricts the pupil; the dilatorpupillae m. is a thin layer of myoepithelial cells with sympathetic innervation that dilate the pupil

Lab 20 – Special SensesI.II.III.IntroductionKeywordsSlidesA. Eyeciliarybody1. General structure2. Lens3. Layersa. Fibrous tunici. Corneaii. Sclerab. Vascular tunici. Irisii. Ciliary bodyiii. Choroidc. Neural tunic (Retina)4. Optic NerveB. EyelidC. Ear1. General structure2. PartsIV.Slide 78: Eye, H&EIUSM – 2016a. External earb. Inner earSummaryciliary processesscleralvenous sinus(canal of Schlemm)and trabecular meshworkirislensthe ciliary body controls the shape of the lens and produces aqueous humor; it is composed of the ciliarymuscle and the ciliary processes; the ciliary muscle is attached to the lens via zonular fibers from between theciliary processes to form the suspensory ligament; during accommodation, contraction of the ciliary musclereleases tension on the ligament causing the lens to thicken permitting focus on near objects; the ciliaryprocesses are covered by a double layer of columnar epithelium which secretes aqueous humor into theposterior chamber; the aqueous humor then passes through the pupil to enter the anterior chamber where itdrains into the trabecular meshwork and the scleral venous sinus to return to the systemic circulation

Lab 20 – Special SensesII.III.IntroductionKeywordsSlidesA. Eye1. General structure2. Lens3. Layersa. Fibrous tunici. Corneaii. Sclerab. Vascular tunici. Irisii. Ciliary bodyiii. Choroidthe choroid contains looseCT, vasculature, andmelanocytesposterior cavity(vitreous chamber)I.Slide 78: Eye, H&EIUSM – 2016the vasculature providesoxygen and nutrients to theadjacent tissuesthe melanocytes provide thedark coloration of the choroidand serve to absorb scatteredlight and reduce reflectionc. Neural tunic (Retina)4. Optic NerveB. EyelidC. Ear1. General structure2. PartsIV.a. External earb. Inner earSummaryretina(neural tunic)choroid(vascular tunic)sclera(fibrous tunic)

Lab 20 – Special SensesI.II.III.IUSM – 2016IntroductionKeywordsSlidesA. Eye1. General structure2. Lens3. Layersa. Fibrous tunici. Corneaii. Sclerab. Vascular tunici. Irisii. Ciliary bodyiii. Choroidc. Neural tunic (Retina)1. inner limiting layer: basal lamina of Müller cells (glial cells) whichsupport the cells and processes of retina; they extend to the outer limitinglayer, where they form tight/adherens junctions with rods and cones2. nerve fiber layer: axons from ganglionic layer below; axons convergeat the optic disc and collectively leave the eye as the optic nerve3. ganglionic layer: cell bodies of multipolar ganglion cells; axons willcollectively form the optic nerve4. inner plexiform layer: axodendritic connections between bipolarneurons and ganglion cells5. inner nuclear layer: cells bodies of bipolar cells and other neurons;bipolar cells (interneurons) connect photoreceptors to ganglion cells4. Optic Nerve6. outer plexiform layer: axodendritic connections betweenphotoreceptors and neurons from the inner nuclear layer1. General structure8. outer limiting layer: junctions between rods/cones and Müller cellsB. EyelidC. Ear2. PartsIV.posterior cavitySlide 78: Eye, H&Ea. External earb. Inner earSummary7. outer nuclear layer: cell bodies of rods and cones (photoreceptors)9. photoreceptors: photosensitive processes of rods (light) and cones (color)10. pigmented epithelium: melanin-containing cuboidal epithelium; absorbslight, forms part of blood-retina barrier, and aids chromophore recyclingchoroid of vascular tunic

pathway of “information”:Lab 20 – Special SensesI.II.III.IUSM – 2016IntroductionKeywordsSlidesA. Eye1. General structure2. Lens3. Layersa. Fibrous tunici. Corneaii. Sclera12344b. Vascular tunici. Irisii. Ciliary bodyiii. Choroidc. Neural tunic (Retina)4. Optic NerveB. EyelidC. Ear1. General structure2. PartsIV.a. External earb. Inner earSummaryinhibition of photoreceptors (inhibitory): photons hitchromophore molecules (e.g., retinal in rods) on “stacked”membranes of photoreceptors (rods or cones); the energizedchromophore leads to a confirmation change in its associatedopsin protein (rhodopsin in rods, or one of 3 iodopsins forcones); the opsin activates transducin, leading to decreasedcGMP and closure of Na channels; cell becomes hyperpolarizedand stops releasing neurotransmitter (glutamate) to bipolar cellsbipolar cells are no longer inhibited by photoreceptors; theydepolarize and send action potentials (APs) to ganglion cellsganglion cells conduct APs through axons into optic nerveoptic nerve optic chiasm LGN (thalamus) primaryvisual cortex in occipital lobe of brain “vision”pathway of light:through cornea where it is refracted (air-corneal surface),accounting for 2/3 of eye’s total optical powerthrough anterior cavity, where additional refraction occurs;muscles of the iris regulate amount of light passing throughpupil and “entering the eye”through the lens it again is refracted; the lens accounts for1/3 of the eye’s total optical power; ciliary mm. can affectshape of lens (contract to loosen tension on suspensoryligaments to accommodate near vision) affecting refractionthrough vitreous body, transmitting light to retinathrough retinal layers to “deepest” level for photoreceptors3251

Lab 20 – Special SensesI.II.III.IUSM – 2016IntroductionKeywordsSlidesA. Eye1. General structure2. Lens3. Layersa. Fibrous tunici. Corneaii. Sclerab. Vascular tunici. Irisii. Ciliary bodyiii. Choroidc. Neural tunic (Retina)4. Optic NerveSlide 49a (464): Optic Nerve, H&Eoptic nerveoptic disc (blind spot)retinadetachment of the retina fromthe pigemented epithelium is anartifact of slide preparationB. EyelidC. Ear1. General structure2. PartsIV.a. External earb. Inner earSummarynerve fibers from the retina (cell bodies in the ganglionic layer) converge at the optic disc to form the opticnerve (CN II), which carries the sensory information into the brain; because the optic disc consists of bundles ofnerve fibers and lacks photoreceptors, it is a “blind spot” in the visual field

Lab 20 – Special SensesI.II.III.IUSM – 2016IntroductionKeywordsSlidesA. Eye1. General structureSlide 138: Eyelid, H&Eexternal2. Lens3. Layersa. Fibrous tunicskeletal muscle oforbicularis oculi m.,and levator palpebrae m.in upper eyelidi. Corneaii. Sclerab. Vascular tunici. Irisii. Ciliary bodylook here for conjunctivaiii. Choroidc. Neural tunic (Retina)4. Optic NerveB. EyelidC. Ear1. General structure2. PartsIV.a. External earb. Inner earSummarylook here for skin andfollicles of eyelashesinternalthe eyelids provide a protective covering of the anterior surface of the eye; they consist of skin lining the outersurface and an inner core of fibroelastic CT known as the tarsus (Gr. “flat surface”); lining the innermost surfaceof the tarsus is the conjunctiva (mucous membrane) continuous onto the anterior sclera of the eye

Lab 20 – Special SensesI.II.III.Slide 138: Eyelid, H&EIUSM – 2016IntroductionKeywordsSlidesA. Eye1. General structure2. Lens3. Layersa. Fibrous tunici. Corneaii. Sclerab. Vascular tunici. Irisii. Ciliary bodyiii. Choroidc. Neural tunic (Retina)tarsus(tarsal plate)dense fibroelastic CT thatcontributes form andsupport to the eyelid4. Optic NerveB. EyelidC. Ear1. General structure2. PartsIV.a. External earb. Inner earSummarytarsal (Meibomian) glandssebaceous glands which produceoils that form a surface layer ontear film, reducing evaporation,and lubricating ocular surfaceconjunctivamucous membrane with a stratified columnarepithelium w/ goblet cells, providing lubricationof surfaces; covers internal eyelid and anterior(exposed) portion of the sclera

Lab 20 – Special SensesI.II.III.IUSM – 2016IntroductionKeywordsSlidesA. Eye1. General structure2. Lens3. Layersa. Fibrous tunici. Corneaii. Sclerab. Vascular tunici. Irisii. Ciliary bodyiii. Choroidc. Neural tunic (Retina)4. Optic NerveB. EyelidC. Ear1. General structure2. PartsIV.a. External earb. Inner earSummaryFig 23-21, Junqueira, 13th ed.

Lab 20 – Special SensesI.II.III.IUSM – 2016IntroductionKeywordsSlidesA. Eye1. General structure2. Lens3. Layersa. Fibrous tunici. Corneaii. Sclerab. Vascular tunici. Irisii. Ciliary bodyiii. Choroidc. Neural tunic (Retina)4. Optic NerveB. EyelidC. Ear1. General structure2. PartsIV.Slide 25: Auditory Meatus, H&Ea. External earb. Inner earSummaryceruminous glandsspecialized apocrine glandsthat aid in production ofcerumen (earwax)keratinized stratifiedsquamous epitheliumhair follicleauditorymeatusthe external auditory meatus (acoustic canal) is the air-filled canal that transmits sounds collected by theauricle of the ear to the tympanic membrane (eardrum); the canal is lined with skin and contains largeceruminous glands; the outer (lateral) portion of the canal is supported by elastic cartilage, continuous withthe cartilage of the auricle

Lab 20 – Special SensesI.II.III.IUSM – 2016IntroductionKeywordsSlidesSlide 121: Internal Ear, H&EA. Eye1. General structure2. Lens3. Layersa. Fibrous tunici. Corneaii. Sclerab. Vascular tunici. Irisii. Ciliary bodyiii. Choroidc. Neural tunic (Retina)4. Optic NerveB. Eyelidvestibular membranesimple squamous epitheliumstria vascularisvascular epithelium whichsecretes endolymph (high K )basilar membranevascular CT structureC. Ear1. General structure2. PartsIV.a. External earb. Inner earSummarythe bony cochlea (Lt. “snail shell”) of the inner ear arises from the vestibule and makes 2 ¾ turns (more arevisible on slide above since guinea pig, not human); the cochlear canal is divided into three compartments: thescala vestibuli and scala tympani which connect at the apex of the cochlea and are filled with perilymph, andthe central scala media (cochlear duct) which contains the sensory organ of Corti and is filled with endolymph

Lab 20 – Special SensesI.II.III.IUSM – 2016IntroductionKeywordsSlidesA. Eye1. General structure2. LensSlide 121: Internal Ear, H&Evestibular membrane3. Layersa. Fibrous tunici. Corneatectorial membranei. Irishair cellsii. Sclerab. Vascular tunicii. Ciliary bodyiii. Choroidc. Neural tunic (Retina)4. Optic NerveB. Eyelidbasilar membraneC. Ear1. General structure2. PartsIV.a. External earb. Inner earSummarythe spiral organ of Corti is responsible for the sensation of sounds; it rests on the basilar membrane within thescala media; its hair cells (outer and inner) have stereocilia attached to the connective tissue tectorialmembrane; sound energy creates a shearing effect between the basilar and tectorial membranes, causing thehair cells (surrounded by endolymph with high K ) to release neurotransmitters to cells in the spiral ganglion

Lab 20 – Special SensesI.II.III.IUSM – 2016IntroductionKeywordsSlidesA. Eye1. General structure2. Lens3. Layersa. Fibrous tunici. Corneaii. Sclerab. Vascular tunici. Irisii. Ciliary bodyiii. Choroidc. Neural tunic (Retina)4. Optic NerveB. EyelidC. Ear1. General structure2. PartsIV.a. External earb. Inner earSummarypressure waves from the oval window travelthrough the scala vestibuli, causing thevestibular membrane to move, and generatingpressure waves in the scala media; the scalavestibuli connects to the scala tympani throughthe helicotrema at the apex of the cochlea,permitting pressure waves to displace the roundwindow into to middle earpressure waves pass in the scala mediadisplace the basilar membrane; composition ofthe basilar membrane differs according to itslocation and affects its displacement: highfrequency sounds displace it near the ovalwindow while low-frequency sounds travelfarthest and cause displacement at the mostdistal portion, near the helicotremascala vestibuli(perilymph)145scala typmani(perilymph)scala media(endolymph)high K 32outer hair cells (3-5 rows), with stereocilia (no kinocilium) intectorial membrane, depolarize when stereocilia are deformed bymovement of basilar membrane; depolarization causes cells to rapidlyshorten, pulling down on tectorial membrane which pulls and deformsthe stereocilia of the inner hair cellsinner hair cells depolarize if pulled toward longest stereocilium,releasing neurotransmitters that synapse with bipolar neurons inspiral ganglion; these neurons fire APs down their axons which formthe cochlear branch of CN VIII (vestibulocochlear)CN VIII cochlear nuclei sup. olivary nuclei inf. colliculus MGN (thalamus) primary aud. cortex in temporal lobe “hearing”

Lab 20 – Special SensesI.II.III.IUSM – 2016IntroductionKeywordsSlidesCommon Confusion:Lip vs. EyelidLip: movable fold of soft tissue surrounding the mouthA. EyeLook for: (1) internal surface is lined by oral mucosa withnon-keratinized stratified squamous epithelium; (2)transition zone between skin and mucosa is more externalthan in eyelid so skin does not cover anterior surface; (3)mucous labial salivary glands deep to skeletal muscle1. General structure2. Lens3. Layersa. Fibrous tunici. Corneaii. Sclerab. Vascular tunici. Irisii. Ciliary bodyLipiii. ChoroidEyelid: protective covering for the anterior surface of the eyec. Neural tunic (Retina)4. Optic NerveLook for: (1) internal surface is lined by conjunctiva withstratified columnar epithelium and many goblet cells; (2)skin extends over anterior surface; (3) sebaceous tarsalglands deep to skeletal muscle; (4) dense fibroelastic CTtarsal plateB. EyelidC. Ear1. General structure2. PartsIV.a. External earb. Inner earSummaryEyelid

Special Senses Lab 20 – Special Senses IUSM – 2016. I. Introduction. II. Keywords. III. Slides. A. Eye. 1. General structure. 2. Lens. 3. Layers. a. Fibrous tunic

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