Plastics Format Understanding The Differences In PCR
USER BULLETINUnderstanding the Differences in PCRPlastics FormatPublication Number MAN0025596 Revision A.0Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1PCR plate formats . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1PCR tube formats . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6PCR sealing closures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8How to use the PCR Plastics Selection Tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9Quality . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13DescriptionThe items listed throughout this user bulletin are representative of available plastics and otherconsumables. Choosing the correct plastic for your instrument is important for ensuring success. Usethe qPCR and PCR Plastics Selection Tool to find the right plastic for you:http://www.thermofisher.com/findplastics.PCR plate formatsNumber of wells96 wellsMost commonly used.384 wellsFor use with high-throughput automation platforms.For Research Use Only. Not for use in diagnostic procedures.
PCR plastic formatsPCR plate formatsNumber of wells, segmented[1,2]8 wells24 wellsA segmented 96-well plate that can be broken into8‑tube strips.A plate provided with 24 wells.32 wells48 wellsA segmented 96-well plate that can be broken into three32-well plates.[1][2]A plate provided with 48 wells.8 and 32-well PCR plates often come as segmented 96-well plates that can easily be broken apart.Convenient format allows for a lower number of samples.MaterialPolypropylene frame and wells Can withstand rapid changes in temperature. Minimizes absorption of reaction components.2Polycarbonate frame with polypropylene wellsSame polypropylene features but also contains arigid frame compatible with high-throughput roboticapplications.Understanding the Differences in PCR Plastics Format User Bulletin
PCR plastic formatsPCR plate formatsFrame colorAvailable in various colors to provide visual organization and identification of samples.Well color[1]Clear or coloredRecommended for end-point PCRapplications.White Ensures the highest level ofsensitivity for qPCR reactions. Recommended for low copynumber samples.[1]FrostedAlternative to white plastic forqPCR if the fluorescence detector isoversaturated.Refer to qPCR instrument manufacturer for recommendations on well color for proper plate calibration.Skirt typeSkirtedSemi-skirtedNon-skirted Full height panel surrounding theedge of the plate. Short panel around the edge ofthe plate. No panel surrounding the edgeof the plate. Fits securely over a thermalcycler with a raised block. Adequate support duringpipetting. Enhanced mechanical strengthfor use with robotic platforms. Mechanical strength for robotichandling. Fits the block of most thermalcyclers and real-time PCRinstruments.Understanding the Differences in PCR Plastics Format User Bulletin Not suitable for roboticapplications.3
PCR plastic formatsPCR plate formatsAutomation compatible Skirted and semi-skirted plates provide a necessary side surface for grippers to grasp. Polycarbonate-framed plates are able to withstand the forces exerted by grippers.Thermal block fit96-well 0.2 mL (standard profile) Most commonly used. 0.3 mL maximum well volume.96-well 0.1 mL (low profile/fast) Reduced height reducesevaporation and enhancesthermal conductivity. 0.2 mL maximum well volume.384-well Ideal for high-throughputapplications. 40 µL maximum well volume.Alphanumeric labelingPrintedHigh contrast, easy to read.Engraved/moldedMay improve sealing of the outer edges.Deck typeFlat Universal fit with most thermal cyclers. Facilitates sealing and handling.4Raised Required for some thermal cycler lids andinstruments. Balances lid pressure without the need for adapters.Understanding the Differences in PCR Plastics Format User Bulletin
PCR plastic formatsPCR plate formatsFrame notchA modified corner of a PCR plate that some instruments require for optimal fit.BarcodingAllows for easy recording and tracking of samples in high-throughput experiments.Understanding the Differences in PCR Plastics Format User Bulletin5
PCR plastic formatsPCR tube formatsPCR tube formatsTo find the correct PCR plate or tube for your instrument use the qPCR and PCR Plastics SelectionTool: http://www.thermofisher.com/findplastics.Tube formatSingleStrip tubesIndividual PCR tubes with separate or attached capsprovide the flexibility to set up the exact number ofreactions to run.Offered in 8 or 12 tube strips with separate or attachedcaps.Well profile0.1 mL (low profile)0.2 mL (standard profile) Fits 0.1 mL thermal blocks. Fits 0.2 mL thermal blocks. Reduced height minimizes air space above thereaction to reduce evaporation and enhance thermalconductivity. Most commonly used.Tube colorAvailable in various colors to provide visual organization and identification of samples.6Understanding the Differences in PCR Plastics Format User Bulletin
PCR plastic formatsPCR tube formatsCap typeDomedFlat For end-point PCR applications. May be required for instruments with concave lids. For qPCR applications, use optical or ultra-cearcaps. May be required for instruments with flat lids.Cap format[1]SeparateStrip caps can be used with standard or low profile PCRstrip tubes and plates.[1]AttachedCan open and close tubes independently to preventsample contamination.To ensure compatibility select strip tubes and caps from the same brand, Applied Biosystems or Thermo Scientific Understanding the Differences in PCR Plastics Format User Bulletin7
PCR plastic formatsPCR sealing closuresPCR sealing closuresTo find the correct PCR plate or tube for your instrument use the qPCR and PCR Plastics SelectionTool: http://www.thermofisher.com/findplastics.Closure typeSealing filmsCreates a tight seal over wellsto prevent evaporation and crosscontamination.8Sealing foilsCreates a tight seal over wellsto prevent evaporation and crosscontamination. Clear adhesive films—For PCR,economical and sticky. For use with light-sensitivesamples. Optical sealing films—For qPCRapplications, non-tacky pressuresensitive adhesive. Pierceable with pipette tip forsample transfer.Strip capsSeals securely with minimal pressure.Can be used with strip tubes or PCRplates. Domed caps—For PCR. Flat caps—For qPCRapplications, maximizes thepassage of fluorescence signalsand minimize distortion.Understanding the Differences in PCR Plastics Format User Bulletin
PCR plastic formatsHow to use the PCR Plastics Selection ToolHow to use the PCR Plastics Selection Tool1. Go to http://www.thermofisher.com/findplastics.2. Select the instrument manufacturer.3. Select the instrument model.Understanding the Differences in PCR Plastics Format User Bulletin9
PCR plastic formatsHow to use the PCR Plastics Selection Tool4. Select the block format.5. Click Display Results.10Understanding the Differences in PCR Plastics Format User Bulletin
PCR plastic formatsHow to use the PCR Plastics Selection Tool6. Filter results using features in left-hand column.Note: To return to the full list of result, click Reset Filters.Understanding the Differences in PCR Plastics Format User Bulletin11
PCR plastic formatsHow to use the PCR Plastics Selection ToolUnderstanding the filter optionsConsumable Type Choose between plate, tube strip, individual tube, cap strip, seal, and accessory.Number of Wells Choose 8 or 12 for strip tubes and caps. Choose 8, 24, 32, 48, 96, or 384 for plates.Plate Skirt Type Choose skirted, semi-skirted, or non-skirted.Well Profile Choose between standard or low profile/fast.Color Choose from the available plate colors. Common options include clear, white, black, and single orassorted colors.Well Color Choose from the available colors. Common options include clear, white, black, single and assortedcolors.Barcoded Narrow options based on whether or not they have a barcode.Automation Compatible Narrow options based on whether or not they are designed for use with automated roboticapplications.12Understanding the Differences in PCR Plastics Format User Bulletin
PCR plastic formatsQualityQualityProduction The production process is carried out in a Class 100,000 cleanroom under ISO 13485 or ISO 9001guidelines.Materials All PCR plastics are manufactured using medical-grade virgin polypropylene.Design Made with polished tools designed to produce chemical-free and ultra-smooth well surfaces thathelp prevent inhibition and binding.QC testing Each well is visually inspected and electrostatically tested for defects; samples of each lot are runthrough a PCR cycling test to check for sealing and contaminants.Understanding the Differences in PCR Plastics Format User Bulletin13
For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition.The information in this guide is subject to change without notice.DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL,INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT,INCLUDING YOUR USE OF IT.Revision history: Pub. No. MAN0025596RevisionDateA.07 September 2021DescriptionBaseline for this revision history.Important Licensing Information: This product may be covered by one or more Limited Use Label Licenses. By use of this product, you accept the terms andconditions of all applicable Limited Use Label Licenses.TRADEMARKS: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. 2021 Thermo Fisher Scientific Inc. All rights reserved.How to select PCR Plastics 021/06/2823:36:36 en23:18:46.473 01:00thermofisher.com/support thermofisher.com/askaquestionthermofisher.com7 September 2021
Understanding the Differences in PCR Plastics Format User Bulletin. Quality. Production The production process is carried out in a Class 100,000 cleanroom under ISO 13485 or ISO 9001 guidelines. Materials All PCR plastics are manufactured using medical-grade virgin polypropylene.
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