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MACHEREY-NAGELBioanalysisSelection Guidefor DNA, RNA, and protein purification productsn Compact overview of MN Bioanalysis productsn All essential information at a glancen Find the optimal product for your applicationwww.mn-net.comwww.mn-net.com
Selection GuideSelection GuideRNA, DNA, and protein purification from MACHEREY-NAGELSummarySince 1993 MACHEREY-NAGEL has been successfully developing, producing, andworldwide marketing a comprehensive range of ready to use kits and consumables forpurification of nucleic acids (DNA and RNA) and proteins.The company provides innovative bioseparation technologies and exceptionalproducts for a variety of application areas: academic, industrial, clinical, CROs, andgovernmental research, genomics, nucleic acid based molecular diagnostics, geneticidentity (including forensics, veterinary testing, GMO detection / quantification as well asanimal species differentiation), gene expression profiling, gene therapy, and proteomics.This selection guide presents an overview of the broad portfolio of MN products forDNA, RNA, and protein purification. It serves as a guide to find the most suitableproduct for every application from our constantly growing range of MN Bioanalysisproducts.Why choose MN for your life science applicationMN is known as reliable partner for high quality products in sample preparation. Ourproducts cover a broad range of applications and are highly esteemed in leadinglaboratories worldwide. The vast experience of our research team allows MN to offeroptimal solutions for changing requirements and challenges of today’s life science.Product groupNo.PagennPlasmid DNAMolecular biology-grade plasmid DNA1–44Transfection-grade plasmid DNA5–104Endotoxin-free plasmid DNA11–134Plasmid DNA concentration and desalting14–174nnClean upPCR clean up18–236Gel extraction24–266NGS clean up and size selection276Genomic DNA clean up28–296RNA clean up30–326Dye terminator removal336nnRNARNA from cells and tissue34–428MicroRNA43–468RNA, DNA, and protein isolation47–498How to use the selection guideRNA from blood50–5210As seen at the summary on page 3, the products of this selection guide are listed ingroups according to the intended application.Total RNA from FFPE samples53–5410RNA from plant and fungi5510After identifying the target molecule / starting material of your personal interest, followthe corresponding numbers to select the kits which relate to your lab focus.RNA from soil and stool56–6010DNA from blood and biological fluids61–6912cfDNA from plasma70–7412DNA from tissue and cells75–8212DNA from FFPE samples83–8514DNA from forensic samples86–8914DNA from plant and fungi90–9514DNA from microorganisms9616DNA from soil and stool97–9916DNA from food and feed100–10216Direct PCR103–10416Viral RNA / DNA from blood and biological fluids105–11016Viral RNA / DNA and bacterial DNA from clinical samples11116Viral RNA / DNA and bacterial DNA from veterinary samples11216Purification of His-tag proteins113–12518Purification of GST-tag proteins126–12818nnDNAnnViral RNA and DNAnnProtein2www.mn-net.comwww.mn-net.com3
Plasmid DNANo. ProductPlasmid DNAREFStarting material (E. coli culture)FormatBinding capacity1)Typical yield / recoveryElution volume Vector sizeApproximate processing timeFeaturesnnPlasmid DNAMolecular biology-grade plasmid DNA1NucleoSpin Plasmid,NucleoSpin Plasmid (NoLid)740588.10 / .50 / .250,740499.50 / .2501–5 mL (high copy), 6–10 mL (low copy)Mini spin column60 μg25–45 μg50 μL 25 kbp20 min/6 prepsLow protein contamination due to Wash Buffer AW2NucleoSpin Plasmid EasyPure740727.10 / .50 / .2501–5 mL (high copy)Mini spin column35 μg15–30 μg50 μL 25 kbp14 min/6 prepsUltrafast plasmid mini prep Liquid RNase included3NucleoSpin 8 Plasmid,NucleoSpin 8 Plasmid Core Kit2),NucleoSpin 96 Plasmid,NucleoSpin 96 Plasmid Core Kit2)740621 / .5,740461.4,740625.1 / .4 / .24,740616.41–5 mL8-well strip,30 μg4–30 μg75–150 μL 25 kbp45 min/6 strips or plateFlexible format Flexible processing(vacuum / centrifugation / positive pressure) Automation possibleNucleoSpin 96 Flash740618.2 / .4 / .241.1–1.3 mL (high copy), 1.1–3.9 mL (low copy)96-well plate 250 kbp90 min/2 platesNo BAC shearing 496-well plate8 μg (high copy), 1 μg (low copy)5NucleoSpin Plasmid Transfection-grade740490.10 / .50 / .2501–5 mLMini spin column35 μg15–30 µg30–50 μL 25 kbp20 min/6 prepsPatented technology for endotoxin removal Purification inmini format Ultrafast procedure6NucleoSpin 96 Plasmid Transfection-grade,NucleoSpin 96 Plasmid Transfection-gradeCore Kit2)740491.1 / .4 / .24,740492.4 / .241–5 mL96-well plate20 μg5–20 µg100–200 μL 25 kbp45 min/plateNovel technology to diminish endotoxin content Purification in HTP format Ultrafast procedure7NucleoSnap Plasmid Midi740494.10 / .5050 mLSnap off column(vacuum processing)1500 µg250 μg200–500 μL 25 kbp35 min/6 prepsUltrafast procedure New column design (snap off column)for vacuum processing of large sample volumes Patentedtechnology for endotoxin removal8NucleoBond Xtra Midi,NucleoBond Xtra Midi Plus740410.10 / .50 / .100,740412.10 / .50 200 mL (high copy), 400 mL (low copy)Midi gravity flowcolumn800 μg500 μg 300 kbp70 min/prep,30 min/prepLysate clarification and binding in one step NucleoBond Xtra Midi Plus: NucleoBond Finalizer for rapid plasmidprecipitation9NucleoBond Xtra Maxi,NucleoBond Xtra Maxi Plus740414.10 / .50 / .100,740416.10 / .50 600 mL (high copy), 1200 mL (low copy)Maxi gravity flowcolumn2000 μg1000 μg 300 kbp75 min/prep,35 min/prepLysate clarification and binding in one step NucleoBond Xtra Maxi Plus: NucleoBond Finalizer for rapid plasmidprecipitation10NucleoBond Xtra BAC740436.10 / .25250–750 mL (low copy)Maxi gravity flowcolumn150 μg10–150 μg 300 kbp75 min/4 prepsLysate clarification and binding in one stepPlasmid DNAPlasmid DNATransfection-grade plasmid DNAEndotoxin-free plasmid DNA11NucleoBond Xtra Midi EF,NucleoBond Xtra Midi Plus EF740420.10 / .50,740422.10 / .50 200 mL (high copy), 400 mL (low copy)Midi gravity flowcolumn800 μg500 μg 300 kbp85 min/prep,45 min/prepEndotoxin level of 0.05 EU/μg DNA NucleoBond XtraMidi Plus EF: NucleoBond Finalizer for rapid plasmidprecipitation12NucleoBond Xtra Maxi EF,NucleoBond Xtra Maxi Plus EF740424.10 / .50,740426.10 / .50 600 mL (high copy), 1200 mL (low copy)Maxi gravity flowcolumn2000 μg1000 μg 300 kbp90 min/prep,50 min/prepEndotoxin level of 0.05 EU/μg DNA NucleoBond XtraMaxi Plus EF: NucleoBond Finalizer for rapid plasmidprecipitation13NucleoBond 96 Xtra EF740430.1 / .41–5 mL96-well plate50 μg2–4 μg (1.5 mL in 96-well plates),10–50 μg (5 mL in glass tubes) 25 kbp, 300 kbp(without NucleoBond Finalizer Plate)120 min/plateEndotoxin level of 0.1 EU/μg DNA NucleoBond FilterPlate for lysate clarification, NucleoBond Finalizer Plate forDNA precipitationPlasmid DNA concentration and desalting14NucleoSnap Finisher Midi,NucleoSnap Finisher Maxi740434.10 / .50,740435.10 / .50DNA eluateSnap off column(vacuum processing)1.5 mg90–100 % 100 μL 25 kbp 10 min/12 prepsNo time consuming isopropanol precipitation New columndesign (snap off column) for vacuum processing of largesample volumes15NucleoSpin Finisher Midi740439.10 / .50DNA eluateFunnel column(centrifugeprocessing)1.5 mg90–100 % 100 μL 25 kbp15 min/6 prepsNo time consuming isopropanol precipitation16NucleoBond Finalizer,NucleoBond Finalizer Plus740519.20,740520.205 mL DNA eluateSyringe filter500 μg60–90 %200–800 μL2–50 kbp5 min/prepFast plasmid precipitation NucleoBond Finalizer Plus:additional syringes included17NucleoBond Finalizer Large,NucleoBond Finalizer Large Plus740418.20,740419.2015 mL DNA eluateSyringe filter2000 μg60–90 %400–1000 μL2–50 kbp5 min/prepFast plasmid precipitation NucleoBond Finalizer LargePlus: additional syringes included1)4Theoretical value; 2) Kit mainly for use on automation platforms, for additional accessories and detailed information see www.mn-net.comwww.mn-net.comwww.mn-net.com5
Clean upNo. ProductClean upREFTypical amount of starting materialFormatBinding capacity1)Typical recoveryElution volume Fragment sizeApproximate processing timeFeaturesnnClean up18NucleoSpin Gel and PCR Clean up740609.10 / .50 / .250 400 μL PCR reaction mixtureMini spin column25 μg70–95 %15–30 μL50 bp–approx. 20 kbp10 min/6 preps2 in 1 kit – PCR clean up and gel extraction19NucleoSpin Gel and PCR Clean up Midi740986.20 4 mL PCR reaction mixtureMidi spin column75 µg70–95 %200–400 µL50 bp–approx. 20 kbp25 min/6 preps2 in 1 kit – PCR clean up and gel extraction Now in Midiprep scale20NucleoSpin Gel and PCR Clean up Maxi740610.20 10 mL PCR reaction mixtureMaxi spin column250 µg70–95 %1000 µL50 bp–approx. 20 kbp30 min/6 preps2 in 1 kit – PCR clean up and gel extraction Now in Maxiprep scale21NucleoSpin 8 PCR Clean up,NucleoSpin 8 PCR Clean up Core Kit2),NucleoSpin 96 PCR Clean up,NucleoSpin 96 PCR Clean up Core Kit2)740668 / .5,740463.4,740658.1 / .2 / .4 / .24,740464.4 100 μL PCR reaction mixture8-well strip,15 μg75–95 %75–150 μL50 bp–10 kbp30 min/6 strips,Flexible format Flexible processing(vacuum / centrifugation / positive pressure) Automation possible22NucleoFast 96 PCR Clean up Kit,NucleoFast 96 PCR Plates743500.4,743100.10 / .5020–300 μL PCR reaction mixture96-well plate23NucleoMag PCR744100.1 / .4 / .24 50 μL PCR reaction mixtureMagnetic beads96-well plate45 min / plate40–95 %25–100 μL 150 bp20 min / plate0.3 μg/μL beads80–95 %25–100 μL150 bp–approx. 10 kbp 40–120 min/96 preps3)Fast procedure (vacuum / centrifugation) Automation possibleEasily adapted to automated useGel extraction24NucleoSpin Gel and PCR Clean up740609.10 / .50 / .250 400 mg agarose gelMini spin column25 μg70–95 %15–30 μL50 bp–approx. 20 kbp10 min/6 preps4)2 in 1 kit – PCR clean up and gel extraction25NucleoSpin Gel and PCR Clean up Midi740986.20 4 g TAE / TBE agarose gelMidi spin column75 µg70–95 %200–400 µL50 bp–approx. 20 kbp25 min/6 preps2 in 1 kit – PCR clean up and gel extraction Now in Midiprep scale26NucleoSpin Gel and PCR Clean up Maxi740610.20 10 g TAE / TBE agarose gelMaxi spin column250 µg70–95 %1000 µL50 bp–approx. 20 kbp30 min/6 preps2 in 1 kit – PCR clean up and gel extraction Now in Maxiprep scale17.5 pg–5 μg nucleic acids in NGS6) reactionmixtureMagnetic beads 80 %10–100 μLTunable (150–800 bp)40–120 min/96 preps3)Convenient magnetic bead technology Easy to adjust forspecific applications or sequencers Optimal scalability formanual and automated processingClean upClean upPCR clean upNGS clean up and size selection27NucleoMag NGS Clean up and Size Select5) 744970.5 / .50 / .500Genomic DNA clean up28NucleoSpin gDNA Clean up XS740904.10 / .50 / .250 400 μL solution containing 2 μg DNAMini spin column(XS design)3 μg60–70 %6–10 μL100 bp–approx. 50 kbp 20 min/6 prepsFor small amounts of genomic DNA (e.g., forensic samples)29NucleoSpin gDNA Clean up740230.10 / .50 / .250 150 μL solution containing 25 μg DNAMini spin column50 μg80–90 %50–100 μL100 bp–approx. 50 kbp 15 min/10 prepsSpecial buffer chemistry for clean up of genomic DNARNA clean up30NucleoSpin RNA Clean up XS740903.10 / .50 / .250 300 μL RNA solution containing 90 μg RNAMini spin column(XS design)110 μg85–95 %5–30 μL 200 nt20 min/6 prepsEasy clean up and concentration of prepurified RNA31NucleoSpin RNA Clean up740948.10 / .50 / .250 200 μL phenol / chloroform extract or reactionmixtureMini spin column200 μg85–95 %40–120 μL 200 nt20 min/6 prepsEasy clean up of prepurified RNA32NucleoSpin RNA Clean up Maxi740910.20 35 mg crude RNAMaxi spin column35 mg85–95 %3–5 mL 200 nt30 min/6 prepsSimple, fast, and convenient clean up of huge RNAamounts740523.10 / .50 / .25020 μL sequencing reaction mixtureMini spin column5 min/prep (excl. hydration)Efficient dye terminator removalDye terminator removal33NucleoSEQ 20 μL1)Theoretical value; 2) Kit mainly for use on automation platforms, for additional accessories and detailed information see www.mn-net.com/HTapplications; 3) Depending on instrument type / setup / configuration. For more detailed infomation regarding the processing time and equipment (e.g., automation platform, purification manifolds), please have a look at www.mn-net.com.; 4) Gel melting time excluded; 5) Not available in the USA;6)Next generation sequencing6www.mn-net.comwww.mn-net.com7
RNARNANo. ProductREFTypical amount of starting materialFormatBinding capacity1)Typical yield / recoveryElution volume Fragment sizeApproximate processing timeFeatures5–30 µL 100 nt18 min/6 prepsFor extra small samples down to single cell analysis gDNA removal column – no DNA digestion needed Noß-mercapthoethanol or TCEP XS columns allow elution in5 μL for highest RNA concentrationnnRNA34NucleoSpin RNA Plus XS740990.10 / .50 / .2501–105 cells, 5 mg human / animal tissueMini spin column(XS design)110 µg0.5–2 µg (105 HeLa cells),0.05–0.2 ng (10 HeLa cells),2.5–8 ng (0.5 µg mouse liver),0.1–0.5 ng (0.5 µg mouse brain)35NucleoSpin RNA Plus740984.10 / .50 / .25010⁷ cultured cells, 10⁹ bacterial cells, 10⁸ yeast cells, 30 mg human / animal tissueMini spin column200 μg40–60 μg (5 x 10⁶ HeLa cells),30–120 μL80–100 μg (20 mg mouse liver),40–70 μg (20 mg mouse kidney),30–60 μg (5 mg mouse spleen) 200 nt20 min/6 prepsFiltration and DNA removal in one step with theNucleoSpin gDNA Removal Column Noß-mercaptoethanol / TCEP necessary36NucleoSpin RNA XS740902.10 / .50 / .2501–10⁵ cells, 5 mg human / animal tissueMini spin column(XS design)110 μg0.1–1.5 ng (10² HeLa cells),1–1.5 μg (10⁵ HeLa cells)5–30 μL 200 nt35 min/6 prepsFor smallest samples rDNase and NucleoSpin Filtersincluded No ß-mercapthoethanol37NucleoSpin RNA740955.10 / .50 / .250 5 x 10⁶ cultured cells, 10⁹ bacterial cells, 10⁸ yeast cells, 30 mg human / animal tissueMini spin column200 μg14 μg (10⁶ HeLa cells),70 μg (10⁹ bacterial cells)30–120 μL 200 nt35 min/6 prepsrDNase and NucleoSpin Filters included38NucleoSpin RNA Midi740962.20 5 x 10⁷ cultured cells, 1010 bacterial cells, 3 x 10⁸ yeast cells, 200 mg human / animaltissueMidi spin column700 μg620 μg (4 x 10⁷ HeLa cells)500–1000 μL 200 nt80 min/4 prepsLarge scale RNA preparation rDNase and NucleoSpin Filters included39NucleoSpin 8 RNA,NucleoSpin 8 RNA Core Kit2),NucleoSpin 96 RNA,NucleoSpin 96 RNA Core Kit2)740698 / .5,740465.4,740709.2 / .4 / .24,740466.4 2 x 10⁶ cells, 20 mg human / animal tissue8-well strip,100 μg20 μg (2 x 10⁶ HeLa cells, 20 mg 50–130 μLmouse liver) 200 nt45 min/6 strips,Flexible format Flexible processing(vacuum / centrifugation / positive pressure) Automation possible rDNase included40NucleoMag RNA744350.1 / .4 2 x 10⁶ cells, 20 mg human / animal tissueMagnetic beads41NucleoZOL740404.200Per mL NucleoZol: 2–106 cultured bacteria / yeast cells, 100 mg human / animal / plant tissue, 0.4 mL (viral) fluidsReagent96-well plate70 min/plate0.4 μg/μL beads 30 μg50–200 μL 200 nt40–120 min/96 preps3)(excl. lysis)rDNase included Easily adapted to automated useTotal RNA: 6–8 μg/mg (liver),3–4 μg/mg (kidney, spleen),0.5–1.5 μg/mg (muscle, brain),4–10 μg/10⁶ (cultured cells)Flexible 10 nt (total RNA), 10–200 nt (smallRNA), 200 nt (large RNA) 1hNo chloroform, no phase separation, easy procedure HighRNA yield and purity from any sample material Small andlarge RNA in one or in separated fractions Combinationwith NucleoSpin RNA Set (mini spin columns) possibleRNARNARNA from cells and tissueLarge RNA: 5–7 μg/mg (liver),3–4 μg/mg ( kidney, spleen),0.5–1.5 μg/mg (muscle, brain),3–8 μg/10⁶ (cultured cells)42NucleoSpin RNA Set for NucleoZOL740406.10 / .50 500 µL NucleoZOL sampleMini spin column200 μg85–95 % (depending on samplequality)60 µL 10 nt (total RNA), 10–200 nt (smallRNA), 200 nt (large RNA) 1hTotal RNA (incl. miRNA) with a simple bind-wash-eluteprocedure Efficient lysis, superior yields Save time andbenefit from the easy and proven handlingMicroRNA43NucleoSpin miRNA740971.10 / .50 / .250 10⁷ cells, 30 mg human / animal tissue, 50 mg plant tissue, 150 μL reaction mixtureMini spin column200 μg100 µg total RNA (10⁷ HeLacells: 10 μg small RNA, 90 μglarge RNA)30–100 μL 18 nt45 min/6 preps (small and largeRNA), 35 min/6 preps (smallRNA only)Optional fractionation of small and large RNA No organicsolvents rDNase and NucleoSpin Filters included44NucleoSpin miRNA Plasma740981.10 / .50 / .250 300 μL plasma / serum ( 900 μL with multipleloading steps)Mini spin column200 μgDepending on sample amountand quality20–50 μL 18 nt40 min/10 preps,70 min/10 preps (incl. DNAdigestion)Efficient purification of RNA Optional co-isolation of cfDNA45Exosome Precipitation Solution(Serum / Plasma)4)740398.2 / .12 / .600.1–1 mL serum / plasmaBuffer set45 min/6 prepsRNA purification from exosomes Simple and efficientprecipitation of exosomes No ultracentrifugation Flexiblescale Ideal for subsequent nucleic acid purification withNucleoSpin miRNA Plasma46Exosome Precipitation Solution (Urine)4)740399.12 / .50 / .2501–10 mL urineBuffer set45 min/6 prepsRNA purification from exosomes Simple and efficientprecipitation of exosomes No ultracentrifugation Flexiblescale Ideal for subsequent nucleic acid purification withNucleoSpin miRNA PlasmaRNA, DNA, and protein isolation47NucleoSpin TriPrep740966.10 / .50 / .250 5 x 10⁶ cells, 30 mg human / animal tissue, 100 mg plant tissueMini spin column200 μg 70 μg RNA, 6 μg DNA, 1200 μg protein40–120 μL(RNA),100 μL (DNA),10–100 μL(protein) 200 nt (RNA), 30 kbp (DNA),15–300 kDa (protein)30 min/6 preps (RNA),RNA, DNA, and proteins - three molecules in separate45 min/6 preps (RNA and DNA), fractions One procedure 35 min/6 preps (protein)48NucleoSpin RNA/Protein740933.10 / .50 / .250 5 x 10⁶ cells, 30 mg human / animal tissue, 100 mg plant tissueMini spin column200 μg 70 μg RNA, 1200 μg protein40–120 μL(RNA),10–100 μL(protein) 200 nt (RNA),15–300 kDa (protein)30 min/6 preps (RNA), 35 min/6 preps (protein)1)8RNA and proteins - two molecules in separate fractions One procedureTheoretical value; 2) Kit mainly for use on automation platforms, for additional accessories and detailed information see www.mn-net.com; 3) Depending on instrument type / setup / configuration. For more detailed infomation regarding the processing time and equipment (e.g., automation platform, purification manifolds), please have a look at www.mn-net.com.; 4) Not available in the USAwww.mn-net.comwww.mn-net.com9
RNARNANo. ProductREFTypical amount of starting materialFormat740944See NucleoSpin RNA, NucleoSpin RNA XS,NucleoSpin miRNA, NucleoSpin RNA Blood,NucleoSpin RNA Plant, NucleoSpin RNA/ProteinBuffer setBinding capacity1)Typical yield / recoveryElution volume Fragment sizeApproximate processing timeFeaturesRNA yield and quality identical toNucleoSpin RNA kits100 μL (DNA) 30 kbp (DNA)5 min/6 preps (DNA), for RNA,see NucleoSpin RNA kitsRNA and DNA – two molecules in separate fractions OneprocedureRNA, DNA, and protein isolation49NucleoSpin RNA/DNA Buffer SetRNA from blood50NucleoSpin RNA Blood740200.10 / .50 400 μL whole blood (fresh or frozen)Mini spin column200 μg1–8 μg (400 μL whole blood)40–120 μL 200 nt55 min/6 prepsNo selective erythrocyte lysis – direct lysis of whole blood rDNase included51NucleoSpin RNA Blood Midi740210.20400–1300 μL whole blood (fresh or frozen)Midi spin column700 μg4–26 μg (1.3 mL whole blood)200–400 μL 200 nt75 min/6 prepsNo selective erythrocyte lysis – direct lysis of whole blood rDNase included52NucleoSpin 8 RNA Blood,NucleoSpin 96 RNA Blood740220 / .5,740225.2 / .4 400 μL whole blood (fresh or frozen)8-well strip,96-well plate100 μg1–8 μg (400 μL whole blood)50–130 μL 200 nt60 min/6 strips,100 min/plateNo selective erythrocyte lysis – direct lysis of wholeblood rDNase included Flexible format Flexibleprocessing (vacuum / centrifugation / positive pressure) Automation possible53NucleoSpin totalRNA FFPE XS740969.10 / .50 / .250 10 sections (10 μm) with 5 mg tissueMini spin column(XS design)100 μgDepending on sample amountand quality5–30 μL70 min/6 preps (90 min incl.optional rDNase digest)Special Paraffin Dissolver (patented technology) – no xylenenecessary – high decrosslinking efficiency54NucleoSpin totalRNA FFPE740982.10 / .50 / .250 10 sections (10 μm) with 50 mg tissueMini spin column200 μgDepending on sample amountand quality30–50 μL70 min/6 preps (90 min incl.optional rDNase digest)Special Paraffin Dissolver (patented technology) – no xylenenecessary – high decrosslinking efficiency740120.10 / .50 / .250 500 mg plant / fungal materialMini spin column200 μg20–70 μg50 μL 200 nt25 min/6 prepsUniversal kit and tailored protocols for challenging plant andfungal samples Convenient handling and efficient isolationof high integrity RNA NucleoSpin RNA Plant and FungiFilters for lysate clearing included600 µg1–10 μg100 μL 100 nt60 min/6 prepsAnion exchange technology to optimize RNA yield andpurity – suitable for metagenomic studies Combinationof mechanical homogenization and chemical lysis for largesample amounts5–50 μg100 μL15 min/12 prepsParallel preparation of RNA and DNA in one hour0.25–2.5 μg50–100 µL60 min/12 prepsRNA purification from soil samples for qRT-PCRanalysis Parallel preparation of RNA and DNA in one hour1.25–12.5 μg50–100 µL15 min/12 prepsParallel preparation of RNA and DNA in one hour10–30 μg (varies by sample andprotocol used)100 μL70 min/10 prepsTotal RNA isolation (incl. miRNA) from human and animalstool samples No Proteinase K treatment required NucleoSpin PCR Inhibitor Removal Columns includedRNA from plant and fungi55NucleoSpin RNA Plant and FungiRNA from soil and stool56NucleoBond RNA Soil740140.20 2 g soilMidi gravity flowcolumn57DNA Set for NucleoBond RNA Soil740141.20NucleoBond RNA Soil kit requiredBuffer set58NucleoBond RNA Soil Mini740142.10 / .500.25–0.5 g soilMini gravity flowcolumn59DNA Set for NucleoBond RNA Soil Mini740143.10 / .50NucleoBond RNA Soil Mini kit requiredBuffer set60NucleoSpin RNA Stool740130.10 / .50180–220 mg fresh or frozen human stool (foranimal stool lower amounts may be required foroptimal results)Mini spin column1) 30 µg200 μg 100 nt 18 ntTheoretical value10www.mn-net.comwww.mn-net.com11RNARNATotal RNA from FFPE samples
DNADNANo. ProductREFTypical amount of starting materialFormatBinding capacity1)Typical yieldElution volume Fragment sizeApproximate processing time FeaturesnnDNA61NucleoSpin Blood740951.10 / .50 / .2505–200 μL blood / serum / plasma, 5 x 106 human / animal cellsMini spin column60 μg4–6 μg (200 μL blood)60–200 μL200 bp–approx. 50 kbp 30 min/prepHigh quality DNA from blood Also suitable for DNA fromplasma, serum, buffy coat, and other body fluids Compatiblewith all blood stabilization substances (e.g., citrate, EDTA,heparin, CPDA)62NucleoSpin Dx Blood (CE-IVD)3)740899.50 / .250200 μL human blood (fresh or frozen, EDTA,citrate, or heparin treated)Mini spin column60 μg3–5 μg (200 μL blood)50–200 μL200 bp–approx. 50 kbp 30 min/prepCE-IVD marked isolation of gDNA from blood Compatiblewith several blood stabilization substances (citrate, EDTA,heparin)63NucleoSpin Blood QuickPure740569.10 / .50 / .2505–200 μL blood / serum / plasma, 5 x 106 human / animal cellsMini spin column50 μg4–6 μg (200 μL blood)30–50 μL200 bp–approx. 50 kbp 25 min/prepFast procedure Washing and drying combined in a singlestep Compatible with all blood stabilization substances (e.g.,citrate, EDTA, heparin, CPDA)64NucleoSpin Blood L740954.20 / .1000.2–2 mL blood / serum / plasma, 2 x 107 human / animal cellsMidi spin column250 μg40–60 μg (2 mL blood)120–200 μL200 bp–approx. 50 kbp 60 min/prepFor processing of larger blood volumes (0.2–2 mL) Compatible with all blood stabilization substances (e.g., citrate, EDTA,heparin, CPDA)65NucleoSpin Blood L Vacuum740954.241–2 mL whole bloodMidi spin column250 µg50–80 μg (2 mL blood)2 x 300 µL300 bp–approx. 50 kbp 75 min/24 prepsParallel processing of 24 samples for time saving workflows(vacuum, positive pressure) Compatible with blood stabilization substances (e.g., citrate, EDTA) Automation possible66NucleoSpin Blood XL740950.10 / .502–10 mL blood / serum / plasma, 108 human / animal cellsMaxi spin column700 μg200–300 μg (10 mL blood)600–2000 μL200 bp–approx. 50 kbp 60 min/prepFor processing of large blood volumes (2–10 mL) Compatiblewith all blood stabilization substances (e.g., citrate, EDTA,heparin, CPDA)67NucleoSpin 8 Blood,NucleoSpin 8 Blood Core Kit2),NucleoSpin 96 Blood,NucleoSpin 96 Blood Core Kit2)740664 / .5,740455.4,740665.1 / .4 / .24,740456.4 200 μL blood / serum / plasma, 2 x 106 human / animal cells8-well strip,20 μg4–6 μg (200 μL blood)100 μL300 bp–approx. 50 kbp 35 min/6 strips (excl. lysis),Flexible format Flexible processing(vacuum / centrifugation / positive pressure) Automationpossible Compatible with blood stabilization substances(e.g., citrate, EDTA, heparin)68NucleoSpin 8 Blood QuickPure,NucleoSpin 96 Blood QuickPure740666 / .5,740667.2 / .4 / .24 200 μL blood / serum / plasma, 5 x 106 human / animal cells8-well strip,96-well plate60 μg4–6 μg (200 μL blood)75–100 μL300 bp–approx. 50 kbp 60 min/12 strips,60 min/2 platesFast procedure and flexible format Manual processing bycentrifuge Compatible with all blood stabilization substances(e.g., citrate, EDTA, heparin, CPDA)69NucleoMag Blood 200 μL,744501.1 / .4,Magnetic beads0.4 μg/μL beads2–8 μg (200 μL blood),50–100 μL,300 bp–approx. 50 kbp 40–120 min/96 preps4),Easily adapted to automated use Compatible with bloodstabilization substances (e.g., citrate, EDTA)NucleoMag Blood 3 mL744502.1 200 μL blood (fresh or frozen, EDTA or citratetreated), 3 mL blood (fresh or frozen, EDTA or citratetreated)100–130 μg (3 mL blood)1000 μL5–30 μL96-well plate70 min/plate (excl. lysis)60 min/24 preps4)cfDNA from plasma70NucleoSpin cfDNA XS740900.10 / .50 / .250 240 μL plasma / serum, 720 μLplasma / serum (multiple loading steps)Mini spin column(XS design)25 pg–25 ng (240 μL plasma)71NucleoSpin cfDNA Midi,NucleoSpin cfDNA Midi Core Kit2)740303.48,740302.481–5 mL plasma (EDTA, Cell-Free DNA BCT )Midi spin column72NucleoSpin 96 cfDNA,NucleoSpin 96 cfDNA Core Kit2)740873.1 / .4,740874.1 / .40.5–2 mL plasma73NucleoSnap cfDNA740300.10 / .5074NucleoMag cfDNA 50 bp20 min/6 prepsSpecial buffer chemistry for cell-free DNA from plasma andserum XS column design for elution in 5 µL for highestconcentrationDepending on sample source,storage, and quality 50 bp90 min/24 prepsSuperior recovery of fragmented cell-free DNA Parallelpurification of 24 samples Special adapter set for NucleoVac96 Vacuum Manifold available Optimized protocol for CellFree DNA BCT (Streck) Automation possible96-well plateDepending on sample source,storage, and quality 50 bp90 min/plateHigh throughput solution for cell-free DNA isolation Optimizedprotocol for Cell-Free DNA BCT (Streck) Manual or automated processing (vacuum / centrifugation / positive pressure)1–10 mL plasma (EDTA, Cell-Free DNA BCT )Snap off columnDepending on sample source,storage, and quality20–100 μL 50 bp45 min/6 prepsNucleoSnap column for quick processing of large samplevolumes by vacuum Highly efficient recovery of nucleic acidsfrom “Liquid Biopsies” No carrier RNA needed744550.1 / .41–10 mL human plasma (EDTA, Cell-Free DNABCT )Magnetic beads0.3 µg/µL beadsDepending on sample source,storage, and quality50–200 µL 50 bp60 min/24 preps (excl. lysis)4)Consistent cfDNA recovery from 1–10 mL plasma samples Efficient purification of fragmented DNA as small as 50 bp Automation possibleDNA from tissue and cells75NucleoSpin DNA RapidLyse740100.10 / .50 / .250 40 mg fresh weight, 106 cellsMini spin column60 μg1–30 μg (depending on sample 60–100 μLsource)200 bp–approx. 50 kbp 25 min/6 preps (excl. lysis)Powerful lysis in one hour or less Unique lysis chemistry forgDNA from cells, tissues, and organs Superior DNA yieldscompared to standard extraction methods76NucleoSpin 96 DNA RapidLyse740110.1 / .4 30 mg fresh weight, 106 cells96-well plate40 μg1–30 μg (depending on sample 100 μLsource)200 bp–approx. 50 kbp 60 min/plate (excl. lysis)4)Unique lysis chemistry for DNA from a variety of sample materials in one hour or less Manual or automated processing byvacuum, positive pressure, or centrifugation Easy automationon robotic platforms77NucleoSpin Tissue XS740901.10 / .50 / .2500.025–10 mg human / animal tissue, 10–104human / animal cells, Guthrie cards (5–30 mm2)Mini spin column(XS design)50 μg0.1–0.5 ng (102 HeLa cells),10–50 ng (104 HeLa cells)5–30 μL200 bp–approx. 50 kbp 20 min/prep (excl. lysis)Purification of genomic, bacterial, and viral DNA from smallestsamples78NucleoSpin Tissue740952.10 / .50 / .250 25 mg human / animal tissue,102–107 human / animal cellsMini spin column60 μg20–35 μg (25 mg mouse liver)60–100 μL200 bp–approx. 50 kbp 20 min/prep (excl. lysis)Allround genomic DNA purification kit for purification fromclinical and forensic samples, tissues, cells, yeast, bacteria,or viruses1)Theoretical value; 2) Kit mainly for use on automation platforms, for additional accessories and detailed information see www.mn-net.com; 3) Not available in the USA; 4) Depending on instrument type / setup / configuration. For more detailed infomation regarding the processing time and equipment (e.g., automation platform, purification manifolds), please have a look at DNADNA from blood and biological fluids
DNADNAREFTypical amount of starting materialFormatBinding capacity1)Typical yieldElution volume Fragment sizeNucleoSpin 8 Tissue,NucleoSpin 8 Tissue Core Kit2),NucleoSpin 96 Tissue,NucleoSpin 96 Tissue Core Kit2)740740 / .5,740453.4,740741.2 / .4 / .24,740454.4 20 mg human / animal tissue, 106 human / animal cells8-well strip,40 μ
8 NucleoBond Xtra Midi, NucleoBond Xtra Midi Plus 740410.10 / .50 / .100, 740412.10 / .50 200 mL (high copy), 400 mL (low copy) Midi gravity flow column 800 μg 500 μg 300 kbp 70 min/prep, 30 min/prep Lysate clarification and binding in one step NucleoBond Xtra Midi Plus: Nucle
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Background Current guidelines for bioanalysis in China －Chinese Pharmacopoeia 2000-2010 －Included in BA/BE guideline (1 page) －CFDA guidelines 2005 Objectives of the new guidance in ChP2015 －A separate guidance for bioanalysis －Harmonized with international guidelines (EMA, FDA) －Detailed information －Meet future demands in China
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Alternative providers, including foreign banks and non-ADIs (‘shadow banks’) have increased lending to some areas of the property sector as the domestic ADIs have pulled back. If this increase in lending by non- . of the availability of finance and its cost rising).
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1003 1.74 1247 1.40 1479 1.18 1849 .0946 2065 0.847 2537 0.690 3045 0.575 3481 0.503 4437 0.394 5133 0.341 6177 0.283 7569 0.231 Ratio 1/8 1/4 1/3 1/2 3/4 1 1.5 2 3 5 7.5 10 15 20 25 30 40 50 60 Motor HP OUTPUT TORQUE lb in min. max. Ratio Output Speed RPM (60 Hz) 1/8 1/4 1/3 1/2 3/4 1 1.5 2 3 5 7.5 10 15 20 25 30 40 50 60 75 100 Motor HP 6 292 8 219 11 159 13 135 15 117 17 103 21 83.3 25 70 .
