Lab Safety Presentation And Contract First Day Of Lab Know .

Lab Safety presentation and contractfirst day of labknow the locations of everything in your labno student can participate in class without signed contractLab inspectionsSafety Office will be randomly inspecting your labsbe sure to use your safety check listChemical control and MSDSimplementation of new MSDS practicesTraining - some is required of everybodysome on line, some liveyou will receive notification

Essentials of Laboratory Safety

Before Lab Work, Get to Know: Hazards of materials & agents andtheir prescribed safety proceduresEmergency spill procedures, use ofadsorbents and disinfectantsDesignated escape route andalternateLocation of fire ext., eye wash,shower, first aid, and spill kitsEmergency telephone numbersand reporting procedures

While Working in the Lab: Shoes with full coverageand good grip solesRestrain long hair, looseclothing and jewelryUse appropriate eye,skin, and handprotection

Eye protection Protects against risk offlying objects or dustparticles, splashes ofhazardous materials orharmful rays

Safety Glasses Unbreakable lenses ofplastic or temperedglassFor light-to-moderateworkCan be prescriptionlensesDo not interfere withcontact lenses

Goggles Work with significant riskof splash of chemicalsor projectilesCan be worn overprescription glasses

Face Shield Work with significant riskof splash on face orpossible explosionFace shield protects faceadequately but not eyes

Hand Protection Protects against risk ofcuts, abrasions, burns,or exposure tohazardous materials.Requires selection of theappropriate chemicalresistant gloves

While Working in the Lab: Handle volatiles in a chemical fume hood Use mechanical transfer devices Follow universal precautions Handle unknowns as if they were hazardous Contain bioaerosols in a biological safety cabinet

While Working in the Lab: Authorized persons onlyIdentify EVERYTHING!No food, beverages,tobacco products, orapplication of cosmetics

While Working in the Lab:Report all: Accidents Injuries Fires Spills Close calls

Before Leaving the Lab:Turn off: Gas Water Power supplies Vacuum lines Compression lines Heating apparatus

Before Leaving the Lab: Identify and packagewaste, dispose properlyLock/out and tag/outdefective equipmentDecontaminate worksurfaces and equipmentReturn unusedequipment, apparatus,etc.Leave lab coat in the labWashClose and lock door

Evaluating Lab Hazards Regular review of thetypes of hazards: chemical physical biological ergonomic mechanical

Weekly Lab Checks Eye wash (purge)Fire extinguisherFirst Aid KitFume HoodTubing, pressurizedconnectionsChemical storage

I. GENERAL SAFETY RULES1. Read the exercise long BEFOREcoming to class. Take note of theinclusion of chemicals and solutions,sharps, glassware, biological hazards,or anything else that might pose asafety concern. Don’t over look littlethings.

2. Under most circumstances in your biologyinstructional labs eye protection is not necessary.However, there are times when you should wheresafety glasses.Wear safetyglasses toprotect your eyesfrom chemicals,heatedmaterials, orthings that mightbe able toshatter.

3. Clean up spills as soon as they happen. Report the spill to yourlab instructor. If the spill is only water, clean it up with paper towels.You may find yourself doing this often around lab bench sinks.http://en.wikipedia.org/wiki/Slip andfall

(3. continued) Large spills should be cleaned up with the spill kit.The kit has a dam to contain the spill and absorbent towels toclean it up with. There is also a plastic bag to put everything inafter the spill is cleaned up.

4. After handling chemicals, alwayswash your hands with soap and water.5. During lab work, keep your handsaway from your face.6. Tie back long hair.7. Roll up loose sleeves.8. Know the location of the fireextinguisher, eyewash station, andfirst aid kit.9. Keep your work area uncluttered.Take to the lab station only what isnecessary.

10. It is suggested that you wear glasses rather thancontact lenses. Contact lenses can trap any chemicalpowders, aerosols, gases or solutions that come incontact with your eyes.

11. Never put anything into your mouth during a labexperiment unless specifically told to do so by yourinstructor.

12. Clean up your lab area at the conclusion of thelaboratory period.

13. Never “horse around” or play practical jokes in thelaboratory.

14. Dispose of animal waste in autoclavable biohazard bags.

15. Dispose of sharps (needles, scalpel blades, etc) in the sharpscontainer.

16. Dispose of paper waste and other trash in the regular trashcontainer.

17. Shorts and open toed shoes are not allowed in lab. You will beasked to leave class if you wear these items to lab. Boots, tennisshoes, or other hard soled shoes are desirable. Long pants orjeans or long dresses are required. Long hair should be tied back.

18. Wash your hands often. In lab and out.

B. GLASSWARE SAFETY1. Chipped or cracked glassware should not be used. Show itto the teacher.2. Broken glassware should not be disposed of in aclassroom trashcan. There is a special glass disposalcontainer for broken glassware and use microscope slides.

3. When pouring liquids into glassware, make sure thecontainer you are pouring into is resting on a table at least ahand’s breadth from the edge.

4.5.If a piece of glassware gets broken, do nottry to clean it up by yourself. Notify the labinstructor.Handle hot glassware with autoclavegloves

6. Handle hot test tubes with test tube holders.

7. Do not place hot glassware in water. Rapidcooling may make it shatter.

8. Wash glassware with dish soap when the exercise is complete

C. CHEMICAL SAFETY1. Wear protective goggles and a labapron whenever heating or pouringhazardous chemicals.

2. Never mix chemicals together unless you are told todo so (and then only in the manner specified).

3. Never taste any chemicals (you should never tasteanything in the lab unless you are specifically told to doso by the lab instructor.).

4. If you need to smell the odor of achemical, waft the fumes toward yournose with one hand. Do not put yournose over the container and inhale thefumes.

5. Never pour water into a concentrated acid. Acidshould be poured slowly into water.

6. Follow the instructions of your labinstructor when disposing of allchemicals. All labs have a specificarea designated for chemical waste.7. Wash your hands after handling anychemicals.

D. ELECTRICAL SAFETY1. Lay electrical cords where no one can tripon them or get caught in them.

2. Be sure your hands and your lab area are drybefore using electrical equipment.

3. Never poke anything into electrical outlets.

4. Unplug cords by pulling the plug and notthe cord.5. Unplug all electrical equipment at the endof the lab period.

E. HEATING SAFETY1. Let burners and hotplates cool downbefore touching them. Test to see ifthey are cool enough by bringing theback of your hand close to them.2. Use tongs and/or protective gloves tohandle hot objects.3. Never reach across an open flame orburner.

4. The only type of glassware that may safelybe heated is either Kimax or Pyrex.5. Always point the top ends of test tubesthat are being heated away from people.6. When heating a test tube, move it aroundslowly over the flame to distribute the heatevenly.

7. Do not heat glassware unless required bythe exercise.8. Heat glassware by placing it on a wiregauze platform on a ringstand or use anelectric hotplate. Do not hold it in yourhand.

9. When lighting a burner, wait until a matchis struck or the striker is in place beforeyou turn on the gas.10. The amount of air can be adjusted by theair supply valve below the tube of theburner. This regulates the flametemperature and color.11. Never leave a burner or hotplateunattended.

FIRST AIDInjury: BurnsTo do: Immediately flush with cold wateruntil burning sensation is lessened.

FIRST AIDInjury: Cuts, bruisesTo do: Do not touch an open woundwithout safety gloves. Pressingdirectly on minor cuts will stopbleeding in a few minutes. Apply coldcompress to bruises to reduceswelling.

FIRST AIDInjury: FaintingTo do: Provide fresh air and have theperson recline so that their head islower than the rest of their body.

FIRST AIDInjury: The eyesTo do: Flush eyes immediately withplenty of water for several minutes. If aforeign object is lodged in the eye, donot allow the eye to be rubbed.

FIRST AIDInjury: PoisoningTo do: Find out what substance wasresponsible for the poisoning and alertthe Lab Instructor immediately.

FIRST AIDInjury: Spills on the skinTo do: Flush with large quantities of water forat least one minute.

FIRST AIDInjury: Electrical shockTo do: Shut off the current at the source.Remove wire with rubber gloves. Alertthe Lab Instructor immediately.

Things to find around the instructional lab:1.2.3.4.5.6.7.8.Fire extinguisherFirst Aid kitEye washBreaker boxSpill kitSoapTable top cleanersPaper towels

Daily Lab ChecksGood Poor CommentsGoggles, or safety glasses as appropriateShoes and long pantsHair tied backNo horse playFirst aid kitFire extinguisher accessibleEye wash readyGas/electric shut offWindows intactSashes downPPE usedGlovesNo food or drinksMSDS availableGas cylinders secured

Lab Safety presentation and contract first day of lab know the locations of everything in your lab no student can participate in class without signed contract Lab inspections Safety Office will be randomly inspecting your l