LaMotte Soil Test Kit Garden Guide - CropWatch
LaMotteSoil Test Kit Garden Guidewww.lamotte.comVisual Symptoms of Element DeficienciesMineralNeedle Color Stem AppearanceRootsNitrogenLight or paleSlender, may bedeficiencygreen colorsucculentSmall rootsPhosphorusdeficiencyYellow colorShort, slender stems Small rootsPotassiumDeficiencyCotyledons redor chocolateShoot dieback;brownstunted growthSmall roots, poorgrowth1
Nutrient Requirements of a Seedling:The minimum quantity of nutrients the plant will absorb to attain thedesired morphological size and physiological condition.Soil Analysis:The major tool for determining the range of critical and acceptablevalues of soil fertility, and for maintaining optimum levels of nutrients.Four Tests: pH, Phosphorus, Nitrogen, PotassiumRecommended Nutrient Levels for Seedling ProductionSoil TextureSandyLoamyClayOrganicMatter (%)1.52.0 3N (%)0.07%0.10%0.15%pH5.5 - 7.55.5 - 7.55.5 - 7.5P (kg/ha)50 - 10075 - 10075 - 125K (kg/ha)75 - 125125 - 175150 - 2502
Soil Sample:1. Must be representative of nursery2. Establish sampling areas3. In one field, collect from 5 areas andmix all samples4. Sample at the root zone5. Keep samples free of foreign objects6. Keep soil management recordsSoil Preparation:1.2.3.4.Spread out sampleAllow soil sample to dry overnightRemove all twigs, etc.Gently crush soil to remove lumpsSoil pH: Why It’s Important1.2.3.4.Numerical scale 1 – 14Measures acidity – alkalinityConifers 5.5 – 6.5 pHDeciduous 6.5 - 8 pH3
Soil pH Test:1. Fill test tube (0755) to line 4with pH Indicator (5701).Squeeze bottle gently.Soil pH Test:2. Use .5 g spoon (0698) to add three measures of soilsample to test tube solution.Soil pH Test:3. Cap and mix gently for oneminute.Soil pH Test:4. Allow test tube to stand for10 minutes to let soil settle.4
Soil pH Test:5. Match color reaction with pH Color ChartPhosphorus: Why It’s Important1. Vital for root formation2. Essential for development of cell tissue3. Vital for development of healthy seeds and fruits5
Soil Phosphorus Test:1. Fill test tube (0755) to line 6 withPhosphorus Extracting Solution(5704)Soil Phosphorus Test:2. Use .5 g spoon (0698) to add three measures of soilsample to test tube solution.Soil Phosphorus Test:3. Cap and mix gently for oneminute.Soil Phosphorus Test:4. Remove cap. Allow to stand,and soil to settle, until liquidabove the soil is clear.6
Soil Phosphorus Test:5. Use one pipet (0364) to transfer the clear liquid to a secondclean test tube. To avoid agitation of the soil, squeeze bulb ofpipet before inserting into liquid. Release bulb slowly to drawclear liquid into pipet. Do not pull any soil. Fill second tube toline 3.Soil Phosphorus Test:6. Add six drops of Phosphorus Indicator Reagent (5705) to soilextract in the second tube.7
Soil Phosphorus Test:8. Add one Phosphorus TestTablet (5707).Soil Phosphorus Test:7. Cap and mixthoroughly.Soil Phosphorus Test:9. Cap and mix until tabletdissolves. A blue color willdevelop.Soil Phosphorus Test:10. Match color withPhosphorus Chart (1372). Low:Medium:High:0 – 50 kg/ha50 – 100 kg/ha 100 kg/ha8
Nitrogen: Why It’s Important1. Part of every living cell (proteins)2. Directly involved in photosynthesis3. Stimulates above-ground growthSoil Nitrogen Test:1. Fill test tube (0755) toline 7 with NitrogenExtracting Solution (5702)Soil Nitrogen Test:2. Use .5 g spoon (0698)to add two measures ofsoil samples.9
Soil Nitrogen Test:3. Cap and mix gently for oneminute.Soil Nitrogen Test:4. Remove Cap and allow soil tosettle.Soil Nitrogen Test:5. Use a clean pipet(0364) to transfer theclear liquid to a secondtest tube. To avoidagitation of the soil,squeeze bulb of pipetbefore inserting tip intoliquid. Release bulbslowly to draw clearliquid into pipet. Do notpull up any soil. Fillsecond tube to line 3with liquid.10
Soil Nitrogen Test:6. Use .25 g spoon (0695) to add twomeasures of Nitrogen Indicator Powder (5703)to soil extract in second tube.Soil Nitrogen Test:7. Cap and gently mix.Wait 5 minutes for pinkcolor to develop abovethe powder.Soil Nitrogen Test:8. Match test colorwith NitrogenColor Chart(1371) Low: 0 – 30kg/haMedium: 30 – 60kg/haHigh: 60 kg/ha11
Potassium: Why It’s Important1. Promotes photosynthesis and efficient use ofwater2. Strengthens natural mechanisms for diseaseresistance, drought tolerance, transplantingshockSoil Potassium Test:1. Fill test tube (0755) toline 7 with PotassiumExtraction SolutionSoil Potassium Test:2. Use .5 g spoon (0698) to add 4measures of soil sample to test tube.12
Soil Potassium Test:3. Cap and shake vigorouslyfor one minuteSoil Potassium Test:4. Remove cap and allow soilto settle.Soil Potassium Test:5. Use a clean pipet(0364) to transfer theclear liquid to asecond clean testtube. Be careful notto pull up any soilinto pipet. Fillsecond tube to line 5with the liquid. Note:If additional extract isneeded to fill the tubeto line 5, repeat steps1 through 4.13
Soil Potassium Test:6. Add one Potassium IndicatorTablet (5708) to soil extract insecond tube.Soil Potassium Test:7. Cap and mix until tablet dissolves.A purplish color will appear.Soil Potassium Test:8. Add Potassium Test Solution (5709), two drops at a time, keepingcount. Mix contents after each addition. Stop adding drops when thecolor changes from purplish to blue.14
Soil Potassium Test:9. Use potassium End Color Chart (1352) as aguide in reading this color change. Keep anaccurate count of the number of drops added.Read test results from table.4 DropsNumber of Drops0-8101214161820 or more Low:Medium:High:Potassium LevelVery highHighMedium HighMediumMedium LowLowVery low8 Drops1 – 120 kg/ha120 – 200 kg/ha 200 kg/ha12 DropsSanitation:1. Wash all equipment after each use.2. Dirty equipment could influence results15
Sources of Soil Enrichment:1. Commercial Fertilizers2. Organic iammoniumphosphateAnhydrousammoniumUreaCalcium NitrateMixed fertilizers,asFormulaNitrogen Content(NH4) N0333.5%(NH4)2 SO421%(NH4)2 HPO421%Liquid NH3CO (NH2)2Ca (NO3)282%45%15%10-10-1010%16
Soil pH Test: 1. Fill test tube (0755) to line 4 with pH Indicator (5701). Squeeze bottle gently. Soil pH Test: 2. Use .5 g spoon (0698) to add three measures of soil sample to test tube solution. Soil pH Test: 3. Cap and mix gently for one minute. Soil pH Test: 4. Al
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Miniprep Kit, the ZR Soil Microbe DNA MiniPrep Kit, the E.Z.N.A. Soil DNA Kit, the FastDNA SPIN Kit for Soil, Norgen Biotek's Soil DNA Isolation Kit or the PureLink Microbiome DNA Purification Kit as described in Experiment 1. DNA purity was assessed by measurements of 260/280 nm and 260/230 nm ratios.
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recommended when soil pH is above 5.3, soil test phosphorus is above 25 ppm, soil test potassium is above 150 ppm, soil test calcium is above 5 meq/100 g soil (1,000 ppm), and soil test magnesium is above 0.5 meq/100 g soil (60 ppm). Do not use
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