Table 1: Quantitative Analysis Of Ceramides Using Liquid .
Table 1: Quantitative Analysis of Ceramides Using Liquid Chromatography Tandem Massspectrometry (LC-MS/MS)ClassQ1 (m/z)Q3 6:0680.95266.4Monohexosyl-ceramides (Glu20d18:1/16:1698.4264.4or Gal 840.31264.4DIhydroGlc or Galceramides32d18:1/16:0702.05266.4(DHGlc or mides (DHCer)SerialMolecularNoSpecies1Quantitative Analysis of Lipids: Dr. Haughey’s LabPage 1
4Table 2: Quantitative Analysis of Sphingomyelins with LC-MS/MSClassSphingomyelinsSerialMolecularQ1 (m/z)Q3 18:1/24:1813.7184.1Quantitative Analysis of Lipids: Dr. Haughey’s LabPage 2
4.1(DHSM)19d18:0/26:0845.7184.1Table 3: Quantitative Analysis of Sulfatides Using LC-MS/MS ularQ1 (m/z)Q3 (m/z)NoSpecies1ST 17:0 (IS)792.796.92ST 16:1776.796.93ST 16:0778.796.94ST 18:1804.796.95ST 18:0806.796.96ST 20:1832.796.97ST 20:0834.796.98ST 22:1860.796.99ST 22:0862.796.910ST 23:0876.696.911ST 24:1888.796.912ST 24:0890.796.913ST 25:1902.796.914ST 26:1916.796.915ST 26:0918.696.916ST 16:0-OH794.796.917ST 18:1-OH820.796.918ST 18:0-OH822.796.919ST 20:0-OH850.796.920ST 22:1-OH876.796.921ST 22:0-OH878.796.922ST 23:0-OH892.796.923ST 24:1-OH904.796.924ST 24:0-OH906.796.925ST 25:1-OH918.796.9Quantitative Analysis of Lipids: Dr. Haughey’s LabPage 3
Table 4: Quantitative Analysis of Fatty Acids Using LC-SRM/MS MethodSerialMolecularQ1 (m/z)Q3 :0255.3255.34C17:0 5C28:0395.3395.326C29:0409.3409.3Quantitative Analysis of Lipids: Dr. Haughey’s LabPage 4
Table 5: Quantitative Analysis of Eicosanoids Using LC-MRM MethodSerialCommon nameAbbreviationQ1 (m/z)NoQ3(m/z)1(d4) Thromboxane B2(d4) TXB23731732(d4) Prostaglandin F2a(d4) PGF2a3571973(d4) Prostaglandin E2(d4) PGE23552754(d4) Prostaglandin D2(d4) PGD23552755(d4) 15-deoxy-Prostalandin J2(d4) 15d-PGJ23192756(d4) 13,14-dihydro-15-keto(d4) dhk PGF2a357295(d4) dhk PGE2355211(d4) dhk PGD2355211Prostaglandin F2a7(d4) 13,14-dihydro-15-ketoProstaglandin E28(d4) 13,14-dihydro-15-ketoProstaglandin D29(d4) Leukotriene B4(d4) LTB433919710(d8) 5-hydroxy-eicosatrienoic acid(d8) 5-HETE32711611(d8) 12-hydroxy-eicosatrienoic acid(d8) 12-HETE32718312(d8) 15-hydroxy-eicosatrienoic acid(d8) 15-HETE32722613(d6) 20-hydroxy-eicosatrienoic acid(d6) 20-HETE32529514(d4) 9-hydroxy-octadecadienoic acid(d4) 9-HODE29917215(d4) 13-hydroxy-octadecadienoic acid(d4) 13-HODE29919816(d7) 5-oxo-eicosatetraenoic acid(d7) 5-oxoETE32320917(d8) 5(6)-epoxy-eicosatetraenoic acid(d8) 5, 6 EET33020218(d8) 8(9)-epoxy-eicosatetraenoic acid(d8) 8, 9 EET32715819(d8) 11(12)-epoxy-eicosatetraenoic(d8) 11, 12 EET327171(d8) 14, 15 EET327226acid20(d8) 14(15)-epoxy-eicosatetraenoicacid21(d4) 9,10-dihydroxy-octadecenoic acid(d4) 9, 10 diHOME31720322(d4) 12,13-dihydroxy-octadecenoic acid(d4) 12, 13 diHOME31718523(d5) Leukotriene C4(d5) LTC463027224(d8) Arachidonic acid(d8) AA31126725(d5) Eicosapentaenoic acid(d5) EPA30626226(d5) Dohexacosaenoic acid(d5) DHA332234276-keto-Prostaglandin F1a6k-PGF1a36916328Thromboxane B2TXB236916929Prostaglandin F2aPGF2a353193Quantitative Analysis of Lipids: Dr. Haughey’s LabPage 5
30Prostaglandin E2PGE235127131Prostaglandin D2PGD23512713211-beta-Prostaglandin F2a11bPGF2a35319333Thromboxane B1TXB137117134Prostaglandin F1aPGF1a35529335Prostaglandin E1PGE135327336Prostaglandin D1PGD135327337Ä17-6-keto-Prostaglandin F1aÄ17 6k-PGF1a36716338Thromboxane B3TXB336716939Prostaglandin F3aPGF3a35119340Prostaglandin E3PGE334926941Prostaglandin D3PGD334926942dihomo Prostaglandin F2adihomo PGF2a38122143dihomo Prostaglandin E2dihomo PGE237929944dihomo Prostaglandin D2dihomo PGD237929945dihomo Prostaglandin J2dihomo PGJ236129946dihomo 15-deoxy-Prostaglandin J2dihomo 15d PGJ2361299476-Prostaglandin E16k PGE1367143486,15-diketo-, 13, 14-dihydro-6, 15 dk-, dh-PGF1a369113Prostaglandin F1a4915-keto-Prostaglandin F1a15k PGE1a3531135015-keto-Prostaglandin F2a15k PGF2a3511135115-keto-Prostaglandin E215k PGE23491135213, 14-dihydro-Prostaglandin F2adh PGF2a3552755313, 14-dihydro-15-keto Prostaglandindhk PGF2a353291dhk PGE2351207dhk PGD2351207F2a5413, 14-dihydro-15-keto ProstaglandinE25513, 14-dihydro-15-keto ProstaglandinD256bicyclo Prostaglandin E2bicyclo PGE23331135711beta-13, 14-dihydro-15-keto-11b dhk PGF2a353113Prostaglandin F2a5819-hydroxy-PGF2a19oh PGF2a3691935920-hydroxy-PGF2a20oh PGF2a3691936019-hydroxy-PGE219oh PGE23671896120-hydroxy-PGE220oh PGE2367189Quantitative Analysis of Lipids: Dr. Haughey’s LabPage 6
622, 3-dinor-11-beta-Prostaglandin F2a2, 3 dinor 11b PGF2a32514563tetranor-Prostanglin F n E Metabolitetetranor-PGEM32729165tetranor 12-hydroxy-eicosatetraenoictetranor 12-HETE265109acid6611-beta-Prostaglandin E211b PGE235127167Prostaglandin K2PGK23492056812S-hydroxy-heptadecatrienoic acid12-HHT2791636911-hydroxy-eicosatetraenoic acid11-HETE3191677011-hydroxy-eicosapentaenoic acid11-HEPE3171217113-hydroxy-docosahexaenoic acid13-HDoHE34322172Prostaglandin A2PGA233327173Prostaglandin B2PGB23332717415-deoxy-Prostaglandin A215d-PGA231527175Prostaglandin J2PGJ23332717615-deoxy-Ä12, 14-PGD215d-PGD23332717715-deoxy-Ä12, 14-PGJ215d-PGJ231527178Isoprostane F2a-IV5-iso PGF2aVI35311579Isoprostane F2a-III8-iso PGF2aIII353193809-hydroxy-eicosatetraenoic acid9-HETE319151819-hydroxy-eicosapentaenoic acid9-HEPE317149828-hydroxy-docosahexaenoic acid8-HDoHE3431098316-hydroxy-docosahexaenoic acid16-HDoHE3432338420-hydroxy-docosahexaenoic acid20-HDoHE34324185Leukotriene B4LTB43351958620-hydroxy-Leukotriene B420oh LTB43511958720-carboxy-Leukotriene B420cooh LTB4365195885, 6 dihydroxy-eicosatetraenoic acid5, 6 diHETE3351638920-trans-Leukotriene B46t LTB43351959012-epi-Leukotriene B412epi LTB4335195916-trans-, 12-epi-Leukotriene B46t, 12epi LTB43351959212-oxo-Leukotriene B412-oxoLTB433317993Leukotriene C4LTC462527294Leukotriene D4LTD449517795Leukotriene E4LTE44383339611-trans-Leukotriene C411t LTC46252729711-trans-Leukotriene D411t LTD4495177Quantitative Analysis of Lipids: Dr. Haughey’s LabPage 7
9811-trans-Leukotriene E411t LTE4438333995-hydroxy-eicosatetraenoic acid5-HETE3191151005-hydroxy-eicosapentaenoic acid5-HEPE3171151017 -hydroxy-docosahexaenoic acid7-HDoHE3431411024-hydroxy-docosahexaenoic acid4-HDoHE3431011039-hydroxy-octadecatrienoic acid9-HOTrE2931711045-hydroxy-eicosatrienoic oic5,15 diHETE335201acid1065(S),6(R)-lipoxin A46R-LXA43511151075(S),6(S)-lipoxin A46S-LXA43511151085(S),14{R)-Lipoxin A414R-LXA4351115109Lipoxin A5LXA5349113110Lipoxin B4LXB4351221111Resolvin E1RvE1349195112Resolvin D1RvD1375141113Neuroprotectin D1PD135915311415-trans Neuroprolectin D115t PD1359153115lOS, 17S-dihydroxy Docosahexaenoic10S,17S-DiHoHE3591538,15 icacid11715-hydroxy-eicosatetraenoic acid15-HETE31921911815-hydroxy-eicosapenlaenoic acid15-HEPE31721911917-hydroxy Docosahexaenoic acid17-HDoHE34324512013-hydroxy-octadecadienoic acid13-HODE29519512113-hydroxy-ocladecatrienoic acid13-HOTrE29319512213-hydroxy-g-octadecatrienoic acid13-HOTre-g29319312315-hydroxy-eicosatrienoic acid15-HETrE3212211248-hydroxy-eicosatetraenoic acid8-HETE3191551258-hydroxy-eicosapenlaenoic acid8-HEPE31712712610-hydroxy Docosahexaenoic acid10-HDoHE3431811278-hydroxy-eicosatrienoic acid8-HETrE321157128Eoxin C4EXC4625272129Eoxin D4EXD4495177130Eoxin E4EXE443833313112-hydroxy-eicosatetraenoic acid12-HETE319179Quantitative Analysis of Lipids: Dr. Haughey’s LabPage 8
13212-hydroxy-eicosapentaenoic acid12-HEPE31717913314-hydroxy-eicosapentaenoic acid14-HDoHE34320513411-hydroxy-eicosapentaenoic acid11-HDoHE3431491359-hydroxy-octadecadienoic acid9-HODE295171136Hepoxilin A3HXA3335127137Hepoxilin B3HXB33351831385-oxo-eicosatelraenoic acid5-oxoETE31720313912-oxo-eicosatelraenoic acid12-oxoETE31715314015-oxo-eicosatetraenoic acid15-oxoETE3171131419-oxo-octadecadienoic acid9-oxoODE29318514213-oxo-octadecadienoic acid13-oxoODE29311314315-oxo-eicosadienoic acid15-oxoEDE32111314420-hydroxy-eicosatelraenoic acid20-HETE31928914519-hydroxy-eicosatelraenoic acid19-HETE31923114618-hydroxy-eicosatetraenoic acid18-HETE31926114717 -hydroxy-eicosaletraenoic acid17-HETE31924714816-hydroxy-eicosaletraenoic acid16-HETE31918914918-hydroxy-eicosapenlaenoic acid18-HEPE3172151505(6)-epoxy-eicosatrienoic acid5,6 EET3191911518(9)-epoxy-eicosatrienoic acid8,9 EET31915515211(12)-epoxy-eicosatrienoic acid11,12 EET31916715314(15)-epoxy-eicosatrienoic acid14,15 EET31921915414,15-epoxy Eicosaletraenoic acid14,15 EpETE31720815517,18-epoxy Eicosatetraenoic acid17,18 EpETE31721515616,17-epoxy Docosapentaenoic acid16,17 EpDPE34319315719,20-epoxy Docosapentaenoic acid19,20 0 DiHDPA361229acid1599(10)-epoxy-octadecenoic acid9,10 EpOME29517116012(13)-epoxy-octadecenoic acid12,13 EpOME2951951615,6-dihydroxy-eicosatrienoic acid5.6 DHET3371451628,9-dihydroxy-eicosatrienoic acid8,9 DHET33712716311, 12-dihydroxy-eicosatrienoic acid11,12 DHET33716716414,15-dihydroxy-eicosatrienoic acid14.15 DHET3372071659, 10-dihydroxy-octadecenoic acid9,10 diHOME31320116612, 13-dihydroxy-octadecenoic acid12,13 diHOME313183167Arachidonic acidAA303259Quantitative Analysis of Lipids: Dr. Haughey’s LabPage 9
168Adrenic acidADA331287169Eicosapentaenoic acidEPA301257170Docohexaenoic acidDHA327229Table 6: Quantitative Analysis of Phospholipids Using LC-MRM MethodSerialMolecular SpeciesNoQ1Q3(m/z)(m/z)1LPC 14:0468.3184.12LPC 15:0482.3184.13LPC 16:1494.3184.14LPC 16:0496.3184.15LPC 17:0510.3184.16LPC 18:2520.4184.17LPC 18:1522.4184.18LPC 18:0524.4184.19LPC 20:4544.4184.110LPC 20:3546.4184.111LPC 22:6568.4184.112LPC 22:0580.4439.413LPC 24:0608.5184.114LPC 26:1634.4184.115LPC 26:0636.5184.116LPC 28:1662.5184.117LPC 28:0664.5184.118LPC 30:2702.5184.119PC 30:0706.6184.120PC 31:2716.6184.121PC 31:1718.6184.122PC 32:3728.5184.123PC 32:2730.6184.124PC 32:1732.6184.125PC 32:0734.6184.126PC 33:3742.6184.127PC 33:2744.6184.128PC 33:1746.6184.129PC 33:0748.6184.1Quantitative Analysis of Lipids: Dr. Haughey’s LabPage 10
30PC 34:4754.6184.131PC 34:3756.6184.132PC 34:2758.6184.133PC 34:1760.6184.134PC 34:0762.6184.135PC 35:5766.6184.136PC 35:4768.6184.137PC 35:3770.6184.138PC 35:2772.6184.139PC 35:1774.6184.140PC 35:0776.6184.141PC 36:6778.5184.142PC 36:5780.6184.143PC 36:4782.6184.144PC 36:3784.6184.145PC 36:2786.6184.146PC 36:1788.6184.147PC 36:0790.6184.148PC 37:6792.6184.149PC 37:5794.6184.150PC 37:4796.6184.151PC 37:3798.6184.152PC 37:2800.6184.153PC 38:7804.6184.154PC 38:6806.6184.155PC 38:5808.6184.156PC 38:4810.6184.157PC 38:3812.6184.158PC 38:2814.6184.159PC 38:1816.6184.160PC 38:0818.6184.161PC 39:6820.6184.162PC 39:5822.6184.163PC 40:8830.6184.164PC 40:7832.6184.165PC 40:6834.6184.166PC 40:5836.6184.1Quantitative Analysis of Lipids: Dr. Haughey’s LabPage 11
67PC 40:4838.6184.168PC 40:3840.6184.169PC 40:2842.6184.170PC 40:1844.6184.171PC 40:0846.6184.172PC-O 30:0692.5184.173PC-O 32:2716.6184.174PC-O 32:1718.5184.175PC-O 32:0720.6184.176PC-O 34:2744.6184.177PC-O 34:1746.6184.178PC-O 34:0748.6184.179PC-O 36:5766.5184.180PC-O 36:4768.6184.181PC-O 36:3770.6184.182PC-O 36:2772.6184.183PC-O 36:1774.6184.184PC-O 38:7790.5184.185PC-O 38:6792.6184.186PC-O 38:5794.6184.187PC-O 38:3798.6184.188PC-O 40:6820.6184.189PC-P 34:1744.6184.190PC-P 36:4766.5184.191PC-P 36:3768.6184.192PC-P 36:2770.6184.193PC-P 36:1772.6184.194LPE 16:0454.3313.395LPE 18:0482.3341.396LPE 18:1480.3339.397LPE 20:4502.3361.398PE 34:1718.5577.599PE 36:1746.6605.6100PE 36:2744.6603.5101PE 36:3742.5601.5102PE 36:4740.5599.5103PE 38:3770.6629.6Quantitative Analysis of Lipids: Dr. Haughey’s LabPage 12
104PE 38:4768.6627.5105PE 38:5766.5625.5106PE 38:6764.5623.5107PE 40:4796.6655.6108PE 40:6792.6651.5109PE 40:7790.5649.5110PE-O 38:5752.6611.5111PE-O 40:5780.6639.5112PE-O 40:7776.6635.5113PE-P 16:0/18:1702.5339.3114PE-P 16:0/18:2700.5337.3115PE-P 16:0/20:3726.5363.3116PE-P 16:0/20:4724.5361.3117PE-P 16:0/22:4752.5389.3118PE-P 16:0/22:5750.5387.3119PE-P 16:0/22:6748.5385.3120PE-P 17:0/20:4738.5361.3121PE-P 17:0/22:6762.5385.3122PE-P 18:0/18:1730.5339.3123PE-P 18:0/18:2728.5337.3124PE-P 18:0/20:3754.5363.3125PE-P 18:0/20:4752.5361.3126PE-P 18:0/20:5750.5359.3127PE-P 18:0/22:4780.5389.3128PE-P 18:0/22:5778.5387.3129PE-P 18:0/22:6776.5385.3130PE-P 18:1/18:1728.5339.3131PE-P 18:1/18:2726.5337.3132PE-P 18:1/18:3724.5335.3133PE-P 18:1/20:4750.5361.3134PE-P 18:1/22:4778.5389.3135PE-P 18:1/22:5776.5387.3136PE-P 18:1/22:6774.5385.3137PE-P 20:0/18:2756.6337.3138PE-P 20:0/20:4780.6361.3139PE-P 20:0/22:5806.6387.3140PE-P 20:0/22:6804.6385.3Quantitative Analysis of Lipids: Dr. Haughey’s LabPage 13
141PE-P 20:1/20:4778.6361.3142PE-P 20:1/22:6802.6385.3143PE-P 36:1730.502589.5144PE-P 36:3726.5585.5145PE-P 38:1758.6617.6146PE-P 38:2756.6615.6147PE-P 38:3754.6613.6148PE-P 38:5750.5609.5149PE-P 40:6776.601635.5150PE-P 40:7774.6633.5151PG 34:2764.6575.5152PG 36:2792.6603.5153PG 36:3790.6601.5154PI 25:0 (IS)730.4453.4155PI 34:1854.6577.5156PI 38:4904.6627.5157PS 24:0 (IS)624.5493.5158PA 24:0 (IS)554.4439.4159PE 24:0 (IS)580.4439.4Quantitative Analysis of Lipids: Dr. Haughey’s LabPage 14
Quantitative Analysis of Lipids: Dr. Haughey’s Lab Page 1 Table 1: Quantitative Analysis of Ceramides Using Liquid Chromatography Tandem Mass spectrometry (LC-MS/MS) Class Serial No Molecular Species Q1 (m/z) Q3 (m/z) Ceramides 1 d18:1/12:0 482.9 264.4 2 d18:1/16:0 538.9 264.4 3 d18:1/18:0 566.3 264.4 4 d18:1/20:0 594.8 264.4
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