MVE Cryopreservation For Life Science - LabRepCo, LLC

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MVE Cryopreservationfor Life Science1HZ &KDUW 09( 7RXFK 6FUHHQ &RQWURO 6\VWHPV 6WDLQOHVV 6WRUDJH 6\VWHPV IRU 09( &U\RSUHVHUYDWLRQ

ContentsTemperature Chart.3Product Selector Guide .3&KDUW 09( 7RXFK 6FUHHQ .4MVE HEco 800 Series Freezers .5MVE HEco 1500 Series Freezers .7MVE HEco 1800 Series Freezers .9Visit www.chartbiomed.com for more information.

Temperature ChartChart MVE is the leading global manufacturer of vacuum insulated products and cryogenic systems. Morethan fifty years ago, we set the standard for storage of biological materials at low temperatures. Today, wecontinue to exceed these standards. Industries from around the world look to Chart MVE for excellence andinnovation. Our solutions empower industries to better utilize cryogenic technology. In this manner, ChartMVE continues to make a vital contribution in today’s biomedical Life Sciences industry.Recommended maximum temperature for storage of biological samples.MATERIAL TO BESTOREDAlgaeBloodCells:Animals / rariesProtozoaViruses: AnimalIn CellsVOLUMECONTAINERCryovialCryovial/Blood BagINVENTORYCONFIGURATIONBoxes or canesBoxes or canes/bag rackCRITICALTEMPERATURE-150 C-150 C0.5 - 1.0 mL0.5 - 500 mL0.5 - 1.0 mL0.5 - 1.0 mLCryovialCryovialStrawBoxes or canesBoxes or canesCanes-150 C-150 C-150 C0.5 - 1.0 mL0.5 - 1.0 mLCryovialCryovialBoxes or canesBoxes or canes-150 C-150 C0.5 - 1.0 mL0.5 - 1.0 mLCryovialCryovialBoxes or canesBoxes or canes-150 C-150 C0.5 - 1.0 mLCryovialBoxes or canes-150 CProduct Selector GuideDo your biologicalsamples need tostore in vapor?YESNONODo you need tostore 6000 or morevials or straws?YESYESDo you want easyaccess to the samplesand the lowest LN2consumption?Choose from the800, 1500, or 1800SeriesYESChoose fromthe MVE SeriesNOYESNODo you need tostore more than1500 vials?Do you require-190 C top boxtemperature?Choose theCryoSystem6000 FAORNONOAre you usingsquare storageboxes?NOChoose from the MVECryoSystem SeriesYESChoose from the MVESC/XC Series

&KDUW 09( 76 7RXFK 6FUHHQ/FX 4DSFFO XJMM GFBUVSF 9 : (SBQI GPS RVJDL WJFX PG 5FNQFSBUVSF BOE -JRVJE /JUSPHFO -FWFM &KDUW 09( 76 employs a variety of advanced features that enable the controller tomonitor and control the environment inside a freezer with a high level of precision.Liquid Nitrogen Level Measurement IBSU .7& 54 uses a differential pressure system to determinethe LN2 level to an accuracy of 0.5 in. (15 mm) and a resolution of 0.1 in. (5.0 mm). Unlike alternative level sensing systems,differential pressure allows the exact level to be measured anddisplayed. Using the simple single point calibration in a range of3.0 in. to 48.0 in. (75 mm to 1220 mm), the patented, selfmaintaining, closed-loop system displays in inches, millimeters,or a percentage full.Automatic Liquid Nitrogen Level ControlThe fully automated LN2 level control system is based on userdefined parameters that can be electronically adjusted over theentire level range. The parameters include Low Level Alarm, LowLevel Fill Point, High Level Fill Point and High Level Alarm. Theredundant Dual Solenoid Valves for overfill protection run on 24VDC, 1.0 amp (max).Liquid UsageThis exclusive feature provides an estimation of liquid usage totrack LN2 consumption and can provide an early failure warningto allow sufficient time to implement corrective action and saveirreplaceable samples.User-Defined AlarmsA total of 1 audio/visual alarms are used to alert the user to anypotential or developing problems. The alarms include: HighTemperatures, Low Temperatures, High Level, Low Level, LiquidUsage, Maximum Fill Time, Gas Bypass, 4UVDL 0QFO MPTFE Temperature Calibrations, Low Battery, Power Failure, Lid Openand Communication Loss.Remote Alarm MonitoringAlarm monitoring includes Global %JTDSFUF Remote AlarmRelay Temperature MeasurementTwo independent temperature measurement channels are employed to accurately measure the temperature across the entirestorage space. The two platinum RTD sensors have an accuracyof 1.0 C and a resolution of 0.1 C. The temperature can be displayed in C, F or K. The single or two point calibration also hasaltitude compensation for the highest accuracy.7HPSHUDWXUH ,QOHW 6HWWLQJV Hot Gas BypassThis unique feature is able to vent warm nitrogen gas from thesupply line before initiating a fill. This prevents warm gas fromentering the freezer space, which helps maintain a stable temperature gradient and increases the efficiency by reducing excessLN2 evaporation.Event Log / Data Storage4tore vital, unalterable, time-stamped data in nonvolatilememory. This is a great tool for assessing freezer performanceand troubleshooting any problems. The memory can store30,000 events, an estimated 10 years of storage capacity. Thedata includes time-stamped temperatures, LN2 level, liquidusage, and any alarms or events.Password SecurityThe multilevel security system comprised of up to ten userspecific programmable passwords and four security levels canbe customized to grant or restrict personnel access to certainmenus and settings.Communication Capabilities0OF independent port can be used to communicate with otherTEC 3000 controllers, a remote PC, serial printer, or other RS- 485 networks and devices. Options include ASCII, MODBUS,Printer and One Fill All Fill (OFAF).(WKHUQHW Capabilities0OF independent 3 port can be used GPS OFUXPSL DPOOFDUJPO

MVE HEco 800 Series FreezersFeaturing the /FX IBSU .7& 5PVDI 4DSFFO The MVE HEco Series is the next generation High Efficiency freezer from Chart BioMedical. With theirunique shroud design, streamlined LN2 plumbing, and vacuum jacketed transfer hose, the MVE HEco800 Series freezers provides efficient use of LN2, making them the most efficient vapor freezersavailable. These next generation high efficiency freezers incorporate hinged work surfaces that fully enclose all electronics and plumbing to enhance overall safety and usability.The technical and visual improvements combination create a more aesthetically pleasing freezer thatoffers greater functionality. As with all of MVE’s cryogenic freezers, the MVE HEco 800 Seriesfreezers are available in three unique sizes and provide maximum storage density as well as thelongest hold time and highest sample security in the industry.Features include: LN2 efficiency Fully enclosed wiring and plumbing Vacuum jacketed transfer hose Dry sample storage -190 C top box temperature Lowest liftover height Single step standard Optional double-tier step Optional battery backup—ask about one part number to orderHEco with battery backup installed!MVE HEco 800 Temperature Test*20.00.0Freezer firstfilledTop BoxMiddle Box-20.0 Standard One-Tier Step Optional Two-Tier StepspTEMPERATURE C-40.0-60.0TemperatureCrosses -135 C13 days a erliquid supplyremoved-80.0-100.0-120.0Liquid SupplyCompletelyRemoved atDay 10Lid Open Test-170 C Max(2 ure Test Graph* Temperature test indicates typical performance of HEco Series freezer with full inventory system and factory recommended level settings. Actual performance may vary withatmospheric conditions and usage.Visit www.chartbiomed.com for more information.

Rack LayoutsSquare Rack Layout (P)791 OS/U LayoutMVE HEco 815P-190MVE HEco 818P-190MVE HEco 819P-190Maximum Storage Capacity1.2 & 2 ml Vials (Internally Threaded)15,60018,20019,500Quantity of Large Racks 100 cell boxes121212Quantity of Mini Racks 25 cell boxes444Number of Shelves per Rack121415LN2 Capacity w/o Inventory L est.370420463LN2 Capacity at Vapor Platform L est.525555Static Hold Time—Vapor days101111Static Hold Time—Liquid Full days est.748492Neck Opening in. (mm)12.5 (317)12.5 (317)12.5 (317)Usable Internal Height in. (mm)26.5 (673)30.7 (781)34.5 (877)Inner Diameter in. (mm)28.8 (731)28.8 (731)28.8 (731)Overall Height in. (mm)49.5 (1257)53.9 (1370)57.70 (1465)Liftover Height in. (mm)40 (1016)43.8 (1115)47.63 (1209)Door Width Requirement** in. (mm)32.0 (813)32.0 (813)32.0 (813)Door Width Requirement, with handles** in. (mm)33.2 (843)33.2 (843)33.2 (843)Weight Empty* lb. (kg)475 (215)495 (225)515 (234)Weight Liquid Full* lb. (kg) est.1134 (514)Blood Bag CapacitiesTotalBags791 OS/U Medsep (25 ml)1,2246204142872044R9951 (50 ml)768612889671284R9953 (250 ml)416410441641044R9955 (500 ml)304476304476PerformanceUnit DimensionsBags/Frame1168 (530)No.FramesTotalBagsBags/Frame1340 4102481285205104380576DF200 (200 ml)236459236459295559DF700 (700 ml)132344176444220544TWO Year Parts Warranty FIVE Year Vacuum Warranty* Without inventory**Minimum width required for vessel to pass through opening. Footprint may vary. Contact Tech Service for detailed drawings.

MVE HEco 1500 Series Freezers/FX IBSU .7& 5PVDI 4DSFFO The MVE HEco 1500 Series freezers provide efficient use of LN2, making them the most efficientvapor freezers available. These next generation high efficiency freezers incorporate a hinged worksurfaces that fully enclose all electronics and plumbing to enhance overall safety and usability. Thetechnical and visual improvements combine to create a more aesthetically pleasing freezer that offersgreater functionality. As with all of MVE’s cryogenic freezers, the MVE HEco 1500 Series freezers areavailable in three unique sizes and provide maximum storage density as well as the longest hold timeand highest sample security in the industry.Features include: -/ efficiency Fully enclosed wiring and plumbing Vacuum jacketed transfer hose Dry sample storage -190 C top box temperature Lowest liftover height Two tier folding step Optional battery backup—askabout one part number toorder HEco with batterybackup installed!MVE HEco 1536P Temperature Test*20FreezerĮƌƐƚ ĮůůĞĚ0Top Box ŽƩŽŵ Ždž-20TEMPERATURE C-40-60-80dĞŵƉĞƌĂƚƵƌĞĐƌŽƐƐĞƐ ͲϭϯϱΣ Ϯϭ ĚĂLJƐ ĂŌĞƌ EϮ ƵƉƉůLJƌĞŵŽǀĞĚ-100-120 ŝĚ KƉĞŶ dĞƐƚ-170 C Max;Ϯ ĂLJƐͿ-140 EϮ ƵƉƉůLJ ZĞŵŽǀĞĚ Ăƚ Day 13-160-180-2000510152025303540DAYSTemperature Test Graph* Temperature test indicates typical performance of HEco Series freezer with fullinventory system and factory recommended level settings. Actual performancemay vary with atmospheric conditions and usage.Visit www.chartbiomed.com for more information.

Rack LayoutsSquare Rack Layout (P)4R9951 Layout (R)MVE HEco 1536P-190MVE HEco 1539P-190MVE HEco 1542R-1901.2 & 2 ml Vials (Internally Threaded)36,40039,20042,000Quantity of Large Racks 100 cell boxes242426Quantity of Mini Racks 25 cell boxes161616Number of Shelves per Rack131414730768768Maximum Storage CapacityPerformanceLN2 Capacity w/o Inventory L est.LN2 Capacity at Vapor Platform L est.115115115Static Hold Time—Vapor days171717Static Hold Time—Liquid Full days est.100104104Neck Opening in. (mm)17.4 (442)17.4 (442)17.4 (442)Usable Internal Height in. (mm)28.8 (732)30.2 (767)30.8 (782)Inner Diameter in. (mm)38.5 (978)38.5 (978)38.5 (978)Overall Height in. (mm)54.7 (1389)56.8 (1442)56.8 (1442)Liftover Height in. (mm)37.3 (947)39.4 (999)39.4 (999)Door Width Requirement** in. (mm)42.0 (1067)42.0 (1067)42.0 (1067)Door Width Requirement, with handles**in. (mm)43.3 (1099)43.3 (1099)43.3 (1099)Weight Empty* lb. (kg)700 (318)700 (318)700 (318)Weight Liquid Full* lb. (kg) est.2000 (907)2100 (953)2100 (953)Blood Bag s/FrameNo.FramesTotalBagsBags/FrameNo.Frames791 OS/U Medsep (25 ml)2,90574153,3208†4153,1848†3984R9951 (50 ml)1,48862481,73672481,68772414R9953 (250 ml)8124203812420376841924R9955 (500 ml)608415260841525764144DF200 (200 ml)496412449641244884122DF700 (700 ml)256464256464264466Unit DimensionsTWO Year Parts Warranty FIVE Year Vacuum Warranty* Without inventory**Minimum width required for vessel to pass through opening. Footprint may vary. Contact Tech Service for detaileddrawings. † Between usable height and clearance. Please refer to specifications.

MVE HEco 1800 Series Freezers/FX IBSU .7& 5PVDI 4DSFFO The MVE HEco 1800 Series freezers provide efficient use of LN2, making them the most efficient vaporfreezers available. When an LN2 fill is initiated, the redesigned plumbing system optimizes the LN2flow and reduces transfer loss, increasing efficiency and LN2 cost savings. The 1800 series providesoption for greater storage in one freezer. These next generation high efficiency freezers incorporate ahinged work surfaces that fully enclose all electronics and plumbing to enhance overall safety and usability. The technical and visual improvements provide an aesthetically more pleasing freezer while offering greater functionality. As with all of MVE’s cryogenic freezers, the MVE HEco 1800 Series freezersprovide maximum storage density as well as the longest hold time and highest sample security in theindustry.Features include: LN2 efficiency Fully enclosed wiring and plumbing Vacuum jacketed transfer hose Dry sample storage -190 C top box temperature Lowest liftover height Two tier folding step Optional battery backup—ask about onepart number to order HEco with batterybackup installed!Temperature Test*0-20Top BoxFreezer firstfilledon Day 0-40Bottom BoxTemperature C-60-80TemperatureCrosses -135 C27 days afterliquid supplyremoved-100-120Liquid SupplyCompletelyRemoved atDay 22Lid Open Test-170 C ature Test Graph* Temperature test indicates typical performance of HEco Series freezer with full inventorysystem and factory recommended level settings. Actual performance may vary withatmospheric conditions and usage.Visit www.chartbiomed.com for more information.

Rack LayoutsSquare Rack Layout (P)4R9951 Layout (R)MVE HEcoMVE HEcoMVE HEcoMVE HEco1879P-1901881R-1901892P-1901894R-1901.2 & 2 ml Vials (Internally Threaded)79,95081,90092,25094,500Number of Racks 100 cell boxes54605460Number of Racks 25 cell boxes30123012Number of Stages per Rack13131515LN2 Capacity L1745174517701770LN2 Capacity Under Tray L318318295295Static Hold Time—Vapor days25252323Static Hold Time—Liquid Full days139139142142Neck Opening in. (mm)24.7 (627)25 (635)24.7 (627)24.7 (627)Usable Internal Height in. (mm)29.5 (749)29.2 (741)34.5 (876)34.2 (868)Inner Diameter in. (mm)56.0 (1422)54.8 (1391)56.0 (1422)54.7 (1389)Overall Height in. (mm)63.7 (1618)61.3 (1556)68.6 (1742)68.6 (1742)44.0 (1118)Maximum Storage CapacityPerformanceUnit DimensionsLiftover Height in. (mm)38.8 (985)38.8 (985)44.0 (1118)Door Width Requirement** in. (mm)60.0 (1524)60.0 (1524)60.0 (1524)60.0 (1524Weight Empty lb. (kg)1712 (781)1721 (781)1721 (781)1721 (781)Weight Liquid Full* lb. (kg)4830 (2191)4830 (2191)4875 (2211)4875 (2211)Blood Bag agsBags/FrameNo.Frames791 OS/U (25 ml)5,86678385,62878046,70488386,43288044R9951 (50 ml)2,95264922,94064903,93684923,92084904R9953 (250 ml)1,58443961,60844021,98053962,01054024R9955 (500 ml)1,10442761,24043101,38052761,5505310DF200 (200 ml)960424098442461,20052401,2305246DF700 (700 ml)5044126544413663051266805136TWO Year Standard Warranty FIVE Year Vacuum Warranty* Without inventory**Minimum width required for vessel to pass through opening. Footprint may vary. Contact Tech Service for detailed drawings.

Chart: Total Cryogenic SolutionsAir SeparationDistribution & StorageBulk Storage TanksDewarsLiquid CylindersFreezersSecurity Through SystemsChart BioMedical2200 Airport Industrial Dr., Ste. 500Ball Ground, GA 30107Ph 770-721-7759 Toll Free 1-800-482-2473Fax mChart Inc. reserves the right to discontinue its products, or change the prices, materials, equipment, quality, descriptions,specifications and/or processes to its products at any time without prior notice and with no further obligation orconsequence. All rights not expressly stated herein are reserved by us, as applicable.Chart BioMedical Ltd.Ph 44(0) 1344 403100 Fax 44(0) 1344 art Japan Co., Ltd.Ph (03) 5776 2670 Fax (03) 5776 2676customerservice.japan@chartindustries.comChart Australia Pty Ltd.Ph 61 2 974 94 333 Fax 61 2 974 94 pyright 2017 Chart IndustriesPN ML-CRYO0042 A

MVE HEco 800 Series Freezers The MVE HEco Series is the next generation High Efficiency freezer from Chart BioMedical. With their unique shroud design, streamlined LN2 plumbing, and vacuum jacketed transfer hose, the MVE HEco 800 Series freezers provides efficient use of LN2,

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