Abstracts Of The 2017 International Poultry Scientific .
Abstracts of the2017 International Poultry Scientific ForumGeorgia World Congress Center, Atlanta, GeorgiaJanuary 30–31, 2017SYMPOSIA AND ORAL SESSIONSMonday, January 30, 2017AbstractNo.PageNo.Milton Y Dendy Keynote Address . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Physiology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .M1–M9 . . . . . . . . . . .Processing & Products I . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .M10–M23 . . . . . . . . . . .Pathology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .M24–M37 . . . . . . . . . . .SCAD I . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .M38–M48 . . . . . . . . . . .Environment Management I . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .M49–M63 . . . . . . . . . . .Environment Management II . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .M64–M73 . . . . . . . . . . .Metabolism & Nutrition I . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .M74–M88 . . . . . . . . . . .247248250254258261266268Metabolism & Nutrition II . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .M89–M98 . . . . . . . . . . . 273Metabolism & Nutrition III . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .M99–M113 . . . . . . . . . . . 276Metabolism & Nutrition IV . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .M114–M123 . . . . . . . . . . . 280Tuesday, January 31, 2017Metabolism & Nutrition V . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .T124–T136 . . . . . . . . . . .SCAD II . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .T137–T143 . . . . . . . . . . .SCAD III . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .T144–T149 . . . . . . . . . . .Metabolism & Nutrition VI . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .T150–T162 . . . . . . . . . . .Metabolism & Nutrition VII . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .T164–T175 . . . . . . . . . . .Metabolism & Nutrition VIII . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .T177–T188 . . . . . . . . . . .POSTER PRESENTATIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . P189–P306 . . . . . . . . . . .IPSF Author Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .282286288289293296299330
ABSTRACTS2017 International Poultry Scientific ForumGeorgia World Congress Center, Atlanta, GeorgiaJanuary 30-31, 2017Milton Y Dendy Keynote AddressB-313 An Optimist's View on How We Will Maintain Broiler Gut Health and Performance in Today's NAE, Food Safety and FDA RegulatoryClimate Charles Hofacre, University of Georgia, Athens, Georgia, USAWe have been growing broilers with the aid of antibiotics for over 40 years. These drugs have helped the broiler industry maintain the birds’intestinal health by many different bacteria insults but primarily Clostridium perfringens. There are many reasons broiler companies may choose to nolonger routinely feed antibiotics in their broiler diets. There are also the new FDA regulations that remove the label claim for many antibiotics for performance enhancement, however, the labels allow for use in disease prevention. All of these changes may result in less routine use of in feed antibiotics.I believe what this means is the broiler producers may have to evaluate alternative non-antibiotic products to maintain broiler intestinal health, alterproduction practices to lessen the intestinal insults and help educate their growers to what they can do to maintain performance and health without theroutine antibiotic use. In addition, we still need to answer the question of whether the reduction in antibiotic use may result in greater intestinal diseaseresulting in greater levels of Salmonella and Campylobacter coming to the processing plant. We will discuss the antibiotic alternative products, thehusbandry requirements and the impact of going to a no antibiotics ever program on bird health, performance and food safety.1Poult. Sci. 96(E-Suppl. 1)247
2ABSTRACTS OF PAPERS*Author presenting paperGS Denotes Graduate Student CompetitionUG Denotes Undergraduate PresentationPhysiologyM1 The mRNA expression of the kappa opioid receptor in thepituitary and ovary of broiler breeder hens. Ashley Stephens*GS, MarthaFreeman, Adam Davis University of GeorgiaThe neuropeptide dynorphin (Dyn) is translated as preprodynorphin (Pdyn)and undergoes post-translational modification to its mature form, Dyn. Evidence suggests that Dyn acts through its receptor, the kappa opioid receptor(KOR), to negatively impact gonadotrophin-releasing hormone (GnRH)release in mammalian species. Previously our laboratory assessed PdynmRNA expression in pituitary and ovarian tissue of broiler breeder hens.Pdyn mRNA was detected in the pituitary as well as the ovary. Follicularexpression was exclusive to granulosa cells with highest expression in theF1 follicle and the prehierarchical follicles. Thus, Pdyn could have an impact on follicular development. In order to elucidate potential functions ofPdyn, the current research assessed the mRNA expression of its receptor,KOR, in the pituitary and in the theca and granulosa tissues of preovulatoryfollicles of broiler breeder hens, 5 and 72 hours post-feeding. Individualtheca and granulosa layers were separated from the F1-F4 follicles. Thecaand granulosa cells were enzymatically separated from one another in thenonhierarchical follicles which were pooled by size into small yellow (5–12mm in diameter) and large white (2–5 mm in diameter) follicle categories.The isolated theca and granulosa cells from each follicle size from 2 birdswere combined to create 5 replicate samples for each cell type from eachfollicle size and post-feeding period. Likewise, the pituitary from 2 birdswas combined to create 7 replicate samples for each post-feeding duration.Total RNA was extracted from the samples and DNase treated for 2-stepreal-time PCR analyses of KOR and GAPDH (control) mRNA expression. Taqman minor groove binding probes and primers for each gene weredesigned using Primer Express (Version 2.0, Applied Biosystems). WhileKOR mRNA was detected in the pituitary tissue, its expression did not differ based on fasting duration. In the F1 and F2 follicles, KOR mRNA wasdetected only in the theca of the F1 follicle 5 hours post-feeding whereasat 72 hours post-feeding, it was expressed only in the granulosa of the F1and F2 follicles. Given that Pdyn and KOR mRNA are both detected in follicular tissue, the results suggest that locally produced Pdyn could regulatefollicular development.Key Words: granulosa, theca, pituitary, dynorphinM2 The impact of a dietary yeast fermentation product derived fromSaccharomyces cerevisiae on semen quality and semen microbiota ofaged White Leghorn roosters Midian Nascimento dos Santos*GS, ReshmaRamachandran, Kelley Wamsley, Aaron Kiess, Christopher McDanielMississippi State UniversityDietary supplementation of yeast fermentation products (YP) derivedfrom Saccharomyces cerevisiae has been examined in broilers and layinghens. However, limited information is available about the impact of YP onrooster reproductive performance. Thus, the objective of this study was todetermine the effects of feeding different levels of YP on rooster semenquality and semen microbiota (yeast and bacteria). A common basal dietwas formulated to meet or exceed NRC recommendations. A commercially available YP was included at either 0, 0.5 (manufacturer recommendations), or 1.0% of the diet. Sand was included in these diets at either 1, 0.5,or 0%, respectively, to keep nutrients provided by the basal diet consistent.Individually caged White Leghorn roosters (n 63), 60 wk of age, were divided equally among the 3 diets. Individual semen samples were collected248weekly (8 wk) and analyzed for the sperm quality index (SQI), semen volume, sperm concentration, and sperm viability. Biweekly, semen sampleswere serially diluted and spread plated to detect yeast as well as total aerobic bacteria. Regression and correlation analyses of semen characteristicswith dietary levels of YP and with microbiota counts, respectively, wereconsidered significant at P 0.10. Dietary YP did not increase yeast perbillion sperm (P 0.17). However, as the dietary levels of YP increased,there was a linear decrease in the SQI (P 0.07) but a linear increase inbacteria per billion sperm (P 0.07). Additionally, yeast per billion spermwas positively correlated with bacteria per billion sperm (P 0.0001, r 0.56). The decrease in SQI may be a result of the increase in bacteria perbillion sperm with supplementation of YP, because the SQI was negativelycorrelated with bacteria per billion sperm (P 0.0001, r -0.30). Additionally, the SQI is a measurement of overall sperm movement, and, becauseall other semen characteristics were unaffected by dietary treatments, YPmay reduce the SQI by reducing sperm motility. However, interestingly,sperm concentration (P 0.0001, r -0.36), live sperm concentration (P 0.0001, r -0.36) and semen volume (P 0.0001, r -0.25) were negativelycorrelated with bacteria per billion sperm. Possibly the increased numberof bacteria per sperm, as a result of YP supplementation, is detrimentalto semen quality due to the bacteria itself or their toxins and products. Inconclusion, it appears that YP supplementation has an indirect and negativeeffect on semen quality because of an increase of bacteria per sperm.Key Words: Semen, Bacteria, Yeast, Sperm quality index, SpermatozoaM3 Relationship between sperm-egg penetration and the durationof fertility in Chinese painted quail hens (Coturnix chinensis) ReshmaRamachandran*GS, Midian Nascimento Dos Santos, Christopher McdanielMississippi State UniversityA better understanding of sperm storage and release from the oviductalsperm storage tubules could yield better fertility in the poultry industry.Chinese painted quail are an excellent model for poultry reproduction research due to their small size and rapid sexual maturity. However, information regarding the duration of fertility and the minimum number of spermegg penetration (SEP) holes for maximum fertility is not available for thisspecies. Therefore, the objective of this study was to determine the durationof fertility and the minimum SEP holes required for maximum fertility.For 60 breeding pairs, males were removed after obtaining at least 3 eggsfor SEP per breeding pair. Afterwards, eggs were collected daily, and, onalternate days, eggs were analysed for SEP and fertility until SEP was 0 for3 consecutive days. For SEP analysis, the perivitelline layer was examinedmicroscopically for sperm holes in a 1.35 mm2 area of the germinal disc.Eggs for fertility analysis were incubated, candled at 10 d and examinedfor hatch residue analysis. Regression analyses were used to examine therelationships of fertility and sperm-egg penetration with days post-male removal. Prior to male removal, the population distribution of SEP holes inindividual eggs was skewed greatly to the right and ranged from 0-1900holes with a median of 72 and a mean of 192 293. Additionally, greatvariation was also seen in the SEP population distribution for hen averageswhich ranged from 0-977 holes with a median of 105 and a mean of 181 224. A quadratic decline in SEP over days post-male removal was obtained,beginning at approximately 300 SEP holes and declining to 0 holes by 9d. However, fertility declined linearly over days post-male removal, beginning at 95% and reaching 0% by 9 d. Therefore, the duration of fertilityPoult. Sci. 96(E-Suppl. 1)
ABSTRACTS OF PAPERSwas 9 d, and the minimum SEP for 95% fertility was approximately 75holes. In conclusion, Chinese painted quail appear to require more SEPto attain maximum fertility when compared to other avian species. It ispossible that because such a large number of sperm are required to insurefertilization that, with each ovulation, the hen may release an excessivenumber of sperm from the sperm storage tubules, making sustained spermstorage very inefficient.Key Words: Sperm-egg penetration, Fertility, Sperm storage, Hen,Chinese painted quailM4 Evaluation of the role of maternally derived antibodies onthe immunomodulatory capacity of Original XPCTM in broilersJuan Suarez Martinez*GS1, Wen Chou1, Rachel Blount1, Luc Berghman1,Donald McIntyre2, Hilary Pavlidis2, John Carey1 1Texas A&M University;2Diamond VThe poultry industry is currently searching for new strategies to maintainproduction levels while eliminating the use of antibiotics and maintainingbird health. This is a result of media and consumer pressure to limit the useof antibiotics in food production. The functional metabolites of Diamond VOriginal XPCTM (XPC) has been identified by our lab as a potential strategyto combat the problems faced in antibiotic free production. The objective ofthis study was to evaluate the role of maternally derived antibodies on theimmunomodulatory capacity of XPC on antigen-specific humoral immuneresponse in broilers. One-day old Ross 308 broilers (180) were randomlyassigned to a factorial arrangement of three vaccination protocols and twodiets (XPC or control (CON)). In the first protocol broiler chicks were immunized with live Newcastle Disease Virus (NDV) B1 strain vaccine atday 1. In the second protocol broiler chicks were vaccinated with live LaSota strain NDV vaccine at day 1. Both protocols received a secondary immunization with live LaSota strain NDV vaccine at day 21. Protocol threeconsisted of delaying immunizing the chicks until the level of maternalantibodies decayed below 2000 NDV titer level. Once the level of maternalantibodies was verified, broilers were immunized with live LaSota strainNDV vaccine. Antigen specific humoral immune response was assessed byNDV-specific IgY using ELISA on days 1, 7, 14, 21, 28, and 35. Cumulative feed conversion ratio, feed consumption, and body weights were alsoassessed. NDV-specific IgY titers were significantly different (P 0.001)on days 21 and 28 between XPC and CON groups, with XPC birds reaching a higher and faster titer level one week post-secondary immunization inprotocol 1. Protocol 3 resulted in birds reaching the highest NDV-titer levelduring the trial with XPC birds reaching a significantly higher NDV-titerlevel than CON birds (P 0.001). No significant differences were observedbetween XPC and CON birds in protocol 2 and both reached the lowestNDV-titer level observed in the trial. Feed conversion was significantlyhigher (P 0.05) in both treatments from protocol 3 compared to protocols1 and 2. There were no significant differences observed in body weights orfeed consumption between XPC and CON. Overall, the results indicate thatthe use of XPC favorably modulated the broiler immune system againstNDV specific adaptive immunity. The level of maternal derived antibodiesappeared to have a role on the impact of Original XPC on the adaptive immune system which is consistent with previous data from our lab.Key Words: Original XPC, Newcastle Disease, antibody titers, maternalantibodies, broilersM5 Relationship between 4 day percentage breast yield andincidence of woody breast and white striping muscle myopathies SaraOrlowski*GS, Joseph Hiltz, Barbara Mallman, Nicholas Anthony Universityof ArkansasEmbryonic and early post hatch muscle development and growth is primarily through hyperplastic growth and accumulation of nuclei throughsatellite cell contribution. Post hatch, muscle development transitionsfrom hyperplasia to hypertrophic growth of fibers. Commercial selectionfor breast yield traditionally occurs at ages targeting hypertrophic ratherthan hyperplastic growth. This has resulted in the production of giant fi-Poult. Sci. 96(E-Suppl. 1)3bers and concomitant challenges regarding muscle myopathies includingwoody breast and white striping. It has been hypothesized that selection forbreast yield during the hyperplastic growth phase will result in an increasein the number of muscle cells and the number of nuclei at hatch and potentially impact muscle characteristics at processing ages. The current studyinvestigates the relationship between 4 day percentage breast yield and incidence and severity of the woody breast and white striping myopathiesbased on sire family relationships in a randomly mated modern commercial broiler population. Average 4 day percent breast yield was calculatedfor 24 sire families (n 12 per sire family) over two separate hatches andseparated into High (highest yielding 8 families 3.6%), Average (middle8 families), and Low (lowest yielding 8 families 3.3%). A separate hatchof birds representing all 24 sire
Georgia World Congress Center, Atlanta, Georgia January 30-31, 2017 1 Milton Y Dendy Keynote Address B-313An Optimist's View on How We Will Maintain Broiler Gut Health and Performance in Today's NAE, Food Safety and FDA Regulatory Climate Charles Hofacre, University of Georgia, Athens, Georgia, USA
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Le genou de Lucy. Odile Jacob. 1999. Coppens Y. Pré-textes. L’homme préhistorique en morceaux. Eds Odile Jacob. 2011. Costentin J., Delaveau P. Café, thé, chocolat, les bons effets sur le cerveau et pour le corps. Editions Odile Jacob. 2010. Crawford M., Marsh D. The driving force : food in human evolution and the future.
Le genou de Lucy. Odile Jacob. 1999. Coppens Y. Pré-textes. L’homme préhistorique en morceaux. Eds Odile Jacob. 2011. Costentin J., Delaveau P. Café, thé, chocolat, les bons effets sur le cerveau et pour le corps. Editions Odile Jacob. 2010. 3 Crawford M., Marsh D. The driving force : food in human evolution and the future.
