FOOD ALLERGENS - Facts SA Food And Allergy Testing

FOOD ALLERGENSThe FACTS Laboratory offers LC-MS, ELISA, and PCR allergen testing. For more information refer to the Allergen Detection Information Sheet.ALLERGEN*Cow’s MilkLC-MS/MSLIMIT OF DETECTIONRANGE OF QUANTIFICATIONLEAD TIME**1 ppm1 – 1000 ppm5Casein1 ppm1 – 1000 ppm5Whey1 ppm1 – 1000 ppm5*Egg1 ppm1 – 1000 ppm5*Soya1 ppm1 – 1000 ppm5Fish1 ppm1 – 1000 ppm5Crustacean1 ppm1 – 1000 ppm5*Wheat1 ppm1 – 1000 ppm5*Glutenfrom wheat, rye, barley1 ppm1 – 1000 ppm5*Peanut1 ppm1 – 1000 ppm5*Almond1 ppm1 – 1000 ppm5Brazil nut1 ppm1 – 1000 ppm5Cashew nut1 ppm1 – 1000 ppm5Hazelnut1 ppm1 – 1000 ppm5Pecan nut1 ppm1 – 1000 ppm5Pistachio nut1 ppm1 – 1000 ppm5Walnut1 ppm1 – 1000 ppm5Mustard1 ppm1 – 1000 ppm5Sesame seedMulti Allergen Screen1 ppm1 – 1000 ppm51ppmN/A141ppmN/A14crustacean, hen’s egg, fish, cow’smilk, mustard, peanut, sesame,soy, wheatTree Nut Screenalmonds, brazil nuts, cashewnuts, hazelnuts, pecan nuts,pistachio nuts, walnutsSANAS accreditation applies to allergen detection and does not extend to the quantification of allergen residues.ALLERGEN*Total MilkLEAD TIME**No known cross-reactivity2.5 – 25.0 ppm70.167 – 4.5 ppm14CaseinNo known cross-reactivity0.5 - 13.5 ppm14*EggNo known cross-reactivity2.5 – 25 ppm7EggNo known cross-reactivity0.5 – 13.5 ppm14LysozymeNo known cross-reactivity0.05 – 4.0 ppm142.5 - 25.0 ppm720 - 160 ppm145 - 80 ppm7*SoyaCrustacean*Gluten* SANAS accredited methodsFACTS CatalogueRANGE OF QUANTIFICATIONPossible cross-reaction to hemp seeds.Whey (β-Lactoglobulin)ELISAMETHOD CROSS REACTIVITYNo known cross-reactivityPlease note that in highly processed products(e.g. refined / bleached / deodorized /hydrolysed), soy may not be detected by theassay. This includes, but is not limited to soysauce, soy oil, HVP.Low levels of cross-reactivity reported tocurcuma, mussels, mustard, beans andarthropods.No known cross-reactivity** Working days# FacilitatedTestPage 1 of 6Uncontrolled when printed or transmitted electronicallyIssue Date: 14.10.2021

Hydrolysed GlutenNo known cross-reactivity*Peanut*AlmondMustardSesame seedALLERGEN1470.75 – 6.0 ppm72.5 – 20 ppm140.5 – 13.5 ppm mustardNo known cross-reactivity2.5 – 20 ppm sesame14METHOD CROSS REACTIVITYLIMIT OF DETECTIONLEAD TIME**Cross reactivity was observed with DNA extractsfrom muscovy duck (Cairina moschata).1.0 ppm fish DNA14*CeleryNo known cross-reactivity0.4 ppm celery DNA14*MustardNo known cross-reactivity0.4 ppm mustard DNA14WheatNo known cross-reactivity 50 pg genomic DNA14RyeNo known cross-reactivity 50 pg rye DNA14Cross reactivity was observed with DNA extractsfrom shagbark hickory (Carya ovata)0.4 ppm pecan DNAMacadamia nutNo known cross-reactivity0.4 ppm Macadamia nut DNA14MolluscNo known cross-reactivity0.4 ppm mollusc DNA14Brazil nutNo known cross-reactivity0.4 ppm brazil nut14*FishPCR10 – 270 ppmPotential cross-reactivity to other legumes(chickpea, lima bean, lentils, fenugreek), greenpea and wheat semolina.Cross-reaction to apricot stone, mahalebcherry, mulberries and other plants from genusPrunus possible.Antibodies cross-react with rapeseed (67%) andpossibly with seeds of other Brassica spp. Crossreactivity to kidney beans, pinto beans, whitebeans and linseed also observed.Pecan nut14All the above methods can be performed on surface swabs and air plates. It is important to note that all swab results are qualitative,regardless of the method, and that the lab is accredited for select swab analyses. For more information on the FACTS laboratory accreditation status, view our SANASaccreditation certificate or contact us for more information.Please Note: In processed food (e.g. heat treatment, dehydration, etc.), proteins may be altered or fragmented, this may have an impacton the recovery/cross reactivity.SCREENING PACKAGESMETHODDETECTLIMIT OFDETECTIONMilk ScreenLC-MS/MSAlmond, Bovine milk, Buffalo milk, Cashew, Coconut,Goat’s milk, Oat, Rice, Sheep’s milk and Soy0.01%14Tree Nut ScreenLC-MS/MSAlmonds, Brazil nuts, Cashew nuts, Hazelnuts, Pecannuts, Pistachio nuts and Walnuts1ppm14Crustacean, Hen’s egg, Fish, Cow’s milk, Mustard,Peanut, Sesame, Soy and Wheat1ppm14LC-MS/MSGelatine, Mammal muscle tissue, Fish, Crustacean,Molluscs, Avian species, Egg and Milk0.01%14LC-MS/MSTropomyosin, casein, ovalbumin, lysozyme C, CollagenType 1 and 20.01%14PACKAGEMulti Allergen ScreenVegan ScreenVegan Wine Screen* SANAS accredited methodsFACTS CatalogueLC-MS/MS** Working days# FacilitatedLEADTIME**TestPage 2 of 6Uncontrolled when printed or transmitted electronicallyIssue Date: 14.10.2021

ANIMAL SPECIES DETECTION & QUANTIFICATIONSPECIES-SPECIFIC - DETECTION IN MIXED INGREDIENT COMMODITIES (DETECTION OF ADULTERATION)ANIMALSPECIES24 animalspecies DNAbasedscreeningMETHODPCR-microarrayAny product / derivative from the24 species listed (e.g. milk fromcow / gelatin from pork) will bedetected.Single meatspeciesquantitativeLC-MS/MSCattle (Beef, Bison) Water buffalo,Pork, Sheep, Goat, Equine (Horse,Donkey), Hare, Rabbit, Chicken,Turkey, Goose, Mallard Duck,Muscovy Duck, Pheasant, Ostrich,Kangaroo, Springbok, Fallow Deer,Red Deer, Canine, Cat, Camel,Reindeer and Roe DeerRANGE OFQUANTIFICATIONLEADTIME**0.1 – 0.5%N/A140.001 – 10%14N/A14(matrix dependent)Beef or Pork or Sheep or ChickenLC-MS/MSGelatinespeciationLIMIT OFDETECTIONSPECIFICITYBovine, Fish and Pork0.001%0.01%All the above methods can be performed on surface swabs and air plates. It is important to note that all swab results are qualitative,regardless of the method, and that the lab is accredited for select swab analyses. For more information on the FACTS laboratory accreditation status, view our SANASaccreditation certificate or contact us for more information.DNA SEQUENCING FOR SPECIES IDENTIFICATION IN SINGLE SPECIES COMMODITIESANIMAL SPECIESMETHODLEAD TIME**DNA sequencingAnimal and Fish species identificationPlease enquireNot suitable for products containing more than one species14FOOD FRAUD AND AUTHENTICITYSPICES & COMMODITIESMETHODMicroscopic assessment of herbs &spices for authenticity and thedetection of adulterantsEXAMPLELEAD TIME**Allspice , Annatto , Basil Leaves , Bay Leaves , Bun spice , Capsicum ,Cocoa powder , Tomato Pomace , Cardamom , Caraway , Celery Seeds ,Cinnamon, Cloves , Celery Coriander , Cilantro , Cumin Seeds Curryleaves , Fenugreek Seeds , Garlic powder, Ginger, Marjoram , MustardSeeds , Nutmeg , Mint Oregano , Paprika , Parsley , Pepper (Black &White) , Rosemary , Saffron , Sage , Sesame seeds , Thyme, Turmeric,etc.14PLEASE ENQUIRE IF THE HERB/SPICE YOU NEED TO AUTHENTICATE ISNOT LISTED ABOVE.14Microscopic assessment of crystallineingredientsMicroscopic detection of starchadulterantsOther* SANAS accredited methodsFACTS Catalogue** Working daysSalt, Sugar and DextroseMaize and Wheat StarchOnion powder, Tea, Whey powder, Bone meal, Cheese, etc.# Facilitated1414TestPage 3 of 6Uncontrolled when printed or transmitted electronicallyIssue Date: 14.10.2021

FOREIGN OBJECT IDENTIFICATIONMETHODEXAMPLEElectron Microscopy, EnergyDispersive X-Ray SpectroscopyUnidentified objects or visual contamination of food products#ScanningLEAD TIME**14HONEY AUTHENTICITY SCREENINGMETHODEXAMPLEHoney Quality and Authenticity TestingInterpretation of results in terms of compliance with the RegulationsRelating to the Grading, Packing and Marking of Honey and Mixtures ofBee Products intended for sale in the Republic of South Africa (R.835/2000).LEAD TIME**17-20Contact us for more informationOIL AUTHENTICITYMETHOD#Fattyacids methyl esters test#Peroxide#DioxinsEXAMPLELEAD TIME**Coconut oil diluted with palm or sunflower oilsOxidative potential of oilsPresence of dioxins in oil STAcolour Capsaicin Testing#GelatinAnimal, Fish, Plant, and Insect speciesidentificationEXAMPLELEAD TIME**Detection of illegal dyes present in spices, oils etc.Detection of illegal presence of melamine in milk and related productsColour and pungency testing of chilli-based spices to detect foradulterationDetermine compliance to processed meat standard (R. 1283)DNA sequencing1212Please Note: In processed food (e.g. canned samples.), DNA may be altered orfragmented. This may lead to unsuccessful species identification.Quantification of common wheat (Triticum aestivum) in durum wheatproducts (Triticum durum)Durum wheato/ro/r1414NUTRITIONAL ANALYSISNUTRITIONAL TESTINGMETHOD#NutritionalanalysisEXAMPLELEAD TIME**To make nutrient content claims, as part of customer requirements, forcompetitive advantage17 - 20Determining vitamin content to make claims as per R.14617 - 20Determining mineral content to make claims as per R.14614Profiling of amino acids to make a protein related claim as per R.146Determine alcohol sugar content e.g. xylitolQuantify single/multiple artificial sweeteners e.g. aspartameDetermination of alcohol content for claims as per R.146Determination of cholesterol content for claims as per R.146Determine whether a GI claim may be alcohols#Artificial sweeteners#Alcohol testing#Cholesterol#Glyceamic Index#Sugar* SANAS accredited methodsFACTS Catalogue** Working days# FacilitatedTestPage 4 of 6Uncontrolled when printed or transmitted electronicallyIssue Date: 14.10.2021

logicaltesting on nonperishables# Microbial species identification#MycotoxinsEXAMPLELEAD TIME**Testing of food products for contamination with heavy metalcontamination or adulterationTesting of food products for contamination with unlegislated or highlevels of pesticides17 - 2017 - 2014Testing of food products for contamination with microorganismsTo determine speciesTesting of food products for contamination with mycotoxinso/r14OTHER ANALYSESMETHOD#Shelf#pH,EXAMPLElife testingTo determine date of durability of a food productBrix, Acidity, Density#GMO#Histamineo/rIndicator of fish spoilageo/ro/rDetermine Antioxidant contento/rDetermine Polyphenol and Catechin contento/rTo determine the amount of sulphur dioxide present in a sample (onlyapplicable to rinse water and food products)14#Antioxidants& Catechins#SO2o/rTo determine whether a food product complies with regulatoryrequirements.and Sorbic acid#Polyphenolso/r10To determine quality aspects of food productsDetermining Caffeine content in order to comply with the Soft DrinkregulationsTo determine whether a food product complies with GMO regulatoryrequirements (In South Africa, this only applies to product containingMaize, Canola, Cotton and Soy)#Caffeine#BenzoicLEAD TIME**CUSTOM ANALYSESShould you require any other analytical support, our multidisciplinary analytical team offers clients a customisable service to test somethingunusual, confirm a hunch, bridge a gap, or simply offer peace of mind.#Facilitated TestingTest results may be generated by FACTS or a laboratory competent in performing the requested analysis.When the analysis is #facilitated, FACTS provides the following services where applicable or as per instruction from the client: Sourcing an appropriate laboratory for sample matrix and objective of analyses.Coordinating sample distribution and analyses.Interpretation of results are in the context of the relevant South African regulations where applicable, with guidance documents,standards, or scientific literature.Presentation of results, background information and interpretations in an easy-to-understand report.The lead times for facilitated testing is subject to change and may be altered by number of samples and laboratory capacity.* SANAS accredited methodsFACTS Catalogue** Working days# FacilitatedTestPage 5 of 6Uncontrolled when printed or transmitted electronicallyIssue Date: 14.10.2021