ÄKTA System Training Guide
ÄKTA system Training GuideExplorer 100 / 10ien cebioschamamersham bioamersesciencamersham bioscienceamershambioscienceÄKTAexplorer
ÄKTAexplorerInjectorBuffersBoxien mamersncebio scieOutletValveUV Detector3 Wavelengthsamersham biosciencePumpsamershambioscienceÄKTAexplorerPump ApH/cellelectrodeBuffer switchingvalveSampleValveSampleholder
ÄKTAexplorer 100In-linefilterInjectionvalve (V1)1 7623 45MixerSystempumpsColumnselectionvalve (V2)UV3 WavelengthdetectorCondConductivitymonitorpHA1 8726345BSamplepump1 87263451 7623 45Reverseflow valve (V7)pH detectorFractioncollectorFrac-9501 87263451 8726345Bufferselectvalve (V6)Outletvalve (V4)1 87263454Sample selectionvalve (V5)Columnselectionvalve (V3)ÄKTAexplorer 100 AirIn-linefilterInjectionvalve (V1)1 7623 45MixerSystempumpsReverseflow valve (V7)Columnselectionvalve (V2)UV3 WavelengthdetectorCondConductivitymonitorpHA1 8726345BAirsensorSamplepump1 87263451 7623 45pH detectorFractioncollectorFrac-9501 87263451 8726345Bufferselectvalve (V6)1 87263454Sample selectionvalve (V5)Columnselectionvalve (V3)Outletvalve (V4)
ÄKTAexplorer 10In-linefilterInjectionvalve (V1)1 7623 45MixerSystempumpsColumnselectionvalve (V2)UV3 WavelengthdetectorCondConductivitymonitorpHA1 8726345BpH detectorFractioncollectorFrac-9501 87263451 8726345Bufferselectvalve (V6)Outletvalve (V4)1 87263454Columnselectionvalve (V3)ÄKTAexplorer 10 SIn-linefilterInjectionvalve (V1)1 7623 45MixerSystempumps3 WavelengthdetectorCondConductivitymonitorpHA1 8726345BSamplepump1 8726345Columnselectionvalve (V2)UVpH detectorFractioncollectorFrac-9501 87263451 8726345Bufferselectvalve (V6)1 87263454Sample selectionvalve (V5)Columnselectionvalve (V3)Outletvalve (V4)
ÄKTAexplorer 10 XTIn-linefilterInjectionvalve (V1)1 7623 45MixerSystempumpsColumnselectionvalve (V2)UV3 WavelengthdetectorCondConductivitymonitorpHAB1 8726345pH detectorFractioncollectorFrac-9501 8726345Bufferselectvalve (V6)1 87263451 87263454Columnselectionvalve (V3)Outletvalve (V4)
PUMP P-900MixerPump APump BProportionValveA2Acid /AlkaliÄ KTA 10 mls/minÄ KTA 100 mls/minA1 B2BufferB1SaltGRADIENT 6 mls/min6 -14 mls/min 30 mls/min30 - 50 mls/min50 - 100 mls/minWaterMIXER0.6 ml mixer2 ml mixer2 mls mixer5 mls mixer12 mls mixer
PUMP P-900 InletsMixerPump BPump ASwitchingValvesBuffer SelectValve IV-908A2A123A11A12A131 84A14B1Water756A16A15B2A17A18Salt
PUMP P-900 Manually Priming Buffer Inlet LinesPump ACommon inletA2Pump BA1Common inletManualFlowpathManualA1 or A2BufferValveA B1 or B2B1B2FlowpathBufferValveBOpenDraw Airout ofBufferLine
PUMP P-900 Back - Wash LinesPump Back-WashLinesSyringe fittingWash OUTIN-LINEone-wayvalveBufferOUTBall outletcheck valveHigh pressurePiston sealPiston20% Ethanol : WaterBackwash pumpdiaphragmBall inletcheck valveWashINBufferIN
ON-LINE FILTERSP-903 ( 10 ml/min Pump )Low Flow HPLC FilterP-901 ( 100 ml/min Pump )High Flow Fibre FilterPolymer SupportLOAD (sample)INJECT 90832Pump73641111LOAD (wash loop)Waste5SampleWaste
INJECTION VALVE INV- asteWASTE
SAMPLE LOADING METHOD1COLUMN2LOADSAMPLE7PUMP3654Needle PortWasteWasteAlways tighten by handwith needle insertedDo Not Overtighten1COLUMNLOADSAMPLE72PUMP3645Luer LockAdapterWasteWasteSuperloop
SUPERLOOPS10 ml50 mlBuffer150 USHWASTEPUMP orSYRINGECOLUMNCOLUMNSampleEtchedGrooveVolumeMax. allowed columnpressureCode No.10 ml50 ml150 ml4 Mpa4 Mpa2 Mpa18-1113-8118-1113-8218-1023-85
INV-907 Injection Valve - Injection PortPEEK Valve BodyInjection Needle PortValve SealFerruleNeedleTubeNeedleNeedleAdapter
INV-907 Injection Valve - Luer e
Fluid DynamicsFlowPEEK TubingHigh Resistanceto FlowLow Resistanceto FlowEndBeginning
GeneralFluid DynamicsFlow - Injecting a LoopThe way we imagine it FlowsThe way it Actually FlowsBubble Segmentation100ul LoopWaste50ul Load60ul Load100ul Load200ul Load Partial Loading Loop No more than 1/2 Loop Volume Total Loading Loop Inject 2X Loop Volume
LOADING A SAMPLE LOOPInjection -950Pump65Waste
LOADING A SUPER LOOPInjection eWasteSample
MULTI-LOADING A SAMPLE LOOPInjection /O4576WashSamplesWaste
MULTI-LOADING A SUPER LOOPColumn12PumpLoadInjectInjection S3 S4 S5 S6S8
DIRECT LOADING ON TO COLUMNColumn1Pump723Inject64Load5Extra Tube1/16” or 1/8”TubingWasteSample
REVERSE FLOW VALVE ( INV-907 )172Pump354ColumnExtra Tube6StopPlugMONITORDownward FlowPosition 1172Pump34ColumnExtra Tube56StopPlugMONITORUpward FlowPosition 2
OUTLET VALVE PV-908DETECTORTo F8F4F3F5F6
0250500mAUFlowthroughFractionationInjectA1 A2 A3 action CollectionWasteStart050100%B
HiTrap Connections1/16” Female1/16” Male18-1112-551/16” Fingertight Fitting1/16” FemaleM6 Male18-1112-57M6 male / 1/16” FemalePhenyl SepharoseHiTrap ColumnM6 MaleM6 Female18-1112-58M6 female / 1/16” maleFlow Cell Inlet1/16” Male
Multiple HiTrap Arrangment ( Series )Phenyl SepharosePhenyl SepharosePhenyl SepharosePhenyl SepharosePhenyl SepharosePhenyl SepharosertocetDetorcetDerctoetDe
ÄKTAexplorer am ers ha m bio scie nce am er sham bio ci n a m e r s h a m b io s c i en c a m e r s h a m b io s c i e n c e ÄKTA system Training Guide Explorer 100 / 10. ÄKTAexplorer am ers ha m bio scie nce am er sham bio ci n a m e r s h a m b io s c ie n c a m e r s h a m b io s c ie Box n c e Monitor Conductivity pH UV Detector 3 .
of KTA Super Stores. Mahalo toBEAU OSHIRO of C&S Wholesale Grocers. . KTA Downtown, Hilo 321 Keawe Street 808-935-3751 KTA Puainako, Hilo 50 East Puainako Street 808-959-9111 KTA Waimea Waimea Center 808-885-8866 KTA Kailua-Kona Kona Coast Shoppin
KTA Newsletter November 2008 4 KTA News Directors Elected KTA’s Council elected Robert Davey, Jr., Thomas Kelliher, Jeffrey Mitchell, and Paul Shaw to three-year terms on the Board of Directors. Shirk Appointed to Board Wanda Shirk of Ulysses, PA, has been
President at KTA Super Stores. Under his leadership, KTA has helped make that happen by buying the cheaper cuts after hotels and restaurants take most of the expensive ones to create a multitude of value-added products like KTA beef and pork lau lau, teri-yaki beef for fundraisers
The design concepts of KTA standards are applied to the systems of the plant. For instance, the KTA 3201.2 provides design rules for pressure and activity retaining boundaries of components and piping systems of the primary circuits, while KTA 3211.2 provides design rules for the others systems.
MANUAL KMH 880/KTA 870 System Install Manual REVISION 3, August, 2002 PART NUMBER 006-10609-0003 Add, delete or replace pages as indicated below and retain all tabs and dividers. Insert this page immediately behind the titl
scope of AD 2000-Merkblatt W 0 Materials in accordance with KTA 3211.1 Materials 1.4550, 1.4580, 1.4541, 1.4571 under the scope of AD 2000-Merkblatt W 0 A 1 Sm W I (1) W II for: - PG 1- small items - integral supports (2) Materials for: 2
C oc kta il Ba rs in y ou r c hoice of s t y l e - m ode r n or v i nt a ge C e ntre piec e s f or low coc kta il t a ble s with flor a l a r r a nge m e nt , t e a l i ght ca ndl e s & mirror bas e s H i g h ba r t a ble s - m a t ching m i ni ce ntre pi e c e s t o t he c oc
SIDE LOCK ADAPTOR FOR STRAIGHT SHANK TOOLS Taper Angle Class AT3 Maximum runout of the internal ID in relation to the external taper 0.005 mm Ordering Example :-KTA BT40SLA06065 / Qty.- 2 Nos. BT D L D1 Locking Screw Description Quantity BT30SLA06065 30 6 65 20 15 40 M 5 M 6 1 BT30SLA08065 3
