Leica ES2 Leica EZ4 Leica EZ4 E Leica EZ4 W User Manual

Instructions for Use (Continued)Intervention from the userThe microscopes of the E Series containno components that can be maintained or repaired by the user. Exceptions arementioned specifically in this manual.Unless otherwise specified in thismanual, repair and service work maybe carried out by authorized Leica techniciansonly.Unauthorized alterations to the instrument or noncompliant use shall void allrights to any warranty claims.MaintenanceLeica E Series microscopes are basicallymaintenance-free. To ensure that italways operates safely and reliably, we recommend that you take the precaution of contacting the responsible service organization.Here you can arrange for periodic inspectionsand conclude a maintenance contract (recommended).Dangers during useThe microscope illumination is in the exemptgroup (risk group 0) according to EN 62471:2008when used according to its intended use. of the illumination equipment – eitherNever look directly into the LED beamwith or without optical instruments – as thisincreases the risk class. Failure to observe thisnotice poses a risk of eye damage.AccessoriesOnly those accessories may be used that arelisted in this user manual or for which safe usehas been confirmed by Leica Microsystems.Danger of infectionDirect contact with eyepieces is a potential transmission method for bacterialand viral infections of the eye. The risk can bekept to a minimum by using personal eyepiecesfor each individual or detachable eyecups.Leica E Series User ManualSafety and Use 13

Leica ES2Leica E Series User ManualLeica ES2 14

Overview: Leica ES211.10 fixed eyepieces for eyeglass wearers22. Carrying handle33. Two-level magnification changer 10 and 30 4. Focusing drive4567Leica E Series User Manual5. Integrated LED incident light illumination6. Integrated LED transmitted illumination7. Switch for incident and transmitted lightLeica ES2 15

Transport, Placement and StorageBuilt-in carrying deviceAll Leica E Series microscopes areequipped with a carrying handle forconvenient and safe transport.Leica E Series User ManualSetting up the microscope1. Place the microscope on a flat tabletop.2. Connect the power supply to a groundedsocket.Packing the device away1. After use, wind up the cable as shownin the illustration.2. Store the instrument so that it is protectedagainst dust.Leica ES2 16

The Correct Interpupillary DistanceThe ideal imageThe interpupillary distance is correctlyset if you see a single circular image fieldwhen looking at a specimen. Note for eyeglass wearersIf you wear glasses, fold the eyecups back –otherwise, fold the eyecups forwards.Setting the eye distance1. Look into the eyepieces.2. Hold the eyepieces with both hands. Pushthe eyepieces together or separate themuntil you see a circular image. You may need a short time to become familiarwith using the instrument. Not to worry – aftera little while, it will become automatic.Leica E Series User ManualLeica ES2 17

Using the LED IlluminatorIluminating the specimen1. Position a specimen in the middle of thebaseplate.2. Switch the two LED illuminators on or off asdesired.Tips on useUse incident light for plastic, opaqueobjects.OOOOUse transmitted light for transmitted lightspecimens or transparent objects.OOUse the combination of both LED illuminators for partially transparent objects.After switching off, the LEDs remain lit forabout another 4 seconds.Leica E Series User ManualLeica ES2 18

FocusingFocusing raises and lowers the stereomicroscope using the focusing drive. Thespecimen is brought into sharp focus as soon asit is in the focal point of the objective.Focusing1. Set the magnification changer to the lowestlevel (10).3. Set the magnification changer to thesecond level (30).4. Use the focusing drive once again for finefocus.The focusing drive can be operated either leftor right-handed.After you have adjusted the imagesharpness at high magnification, itis maintained even if you switch to a lowermagnification (parfocal).2. Set the working distance to approx.100 mm for coarse focusing.Focusing drive torqueLeica E Series User ManualLeica ES2 19

Magnification DisplayYou can read the configured magnification on the right-hand rotary knob ofthe Leica ES2. The additional magnificationprovided by the eyepieces is already incorporated in the scale.Leica E Series User ManualLeica ES2 20

Adjusting the Resistance of the Focus DriveAdjusting the resistanceIs the focusing drive too easy or too difficultto adjust? No problem – the resistance canbe adjusted individually depending on yourpersonal preferences:1. Grip the drive knobs with both handsand turn them towards each other untilthe desired resistance is reached duringfocusing.Leica E Series User ManualLeica ES2 21

Leica EZ4, Leica EZ4 E orLeica EZ4 WLeica E Series User ManualLeica EZ4, Leica EZ4 E or Leica EZ4 W 22

Overview: Leica EZ411.10 fixed eyepieces for eyeglass wearers22. Carrying handle33. Magnification changer4. Focusing drive4567Leica E Series User Manual5. Integrated LED incident light illumination6. Illumination control7. Integrated LED transmitted illuminationLeica EZ4, Leica EZ4 E or Leica EZ4 W 23

Instrument Variant: Leica EZ4 E or Leica EZ4 W1.123410 fixed eyepieces for eyeglass wearers2. Integrated digital camera with SD card slot3. Mode selection panel (EZ4 E or EZ4 W)564. Carrying handle75. Magnification changer86. IR receiver for optional remote control7. Focusing drive9108. Integrated LED incident light illumination9. Illumination control10. Integrated LED transmitted illuminationLeica E Series User ManualLeica EZ4, Leica EZ4 E or Leica EZ4 W 24

Transport, Placement and StorageBuilt-in carrying deviceAll Leica E Series microscopes areequipped with a holder for convenientand safe transport.Leica E Series User ManualSetting up the microscope1. Place the microscope on a flat tabletop.2. Connect the power supply to a groundedsocket.Packing the device away1. After use, wind up the cable as shownin the illustration.2. Store the instrument so that it is protectedagainst dust.Leica EZ4, Leica EZ4 E or Leica EZ4 W 25

The Correct Interpupillary DistanceThe ideal imageThe interpupillary distance is correctlyset if you see a single circular image fieldwhen looking at a specimen. Note for eyeglass wearersIf you wear glasses, fold the eyecups back –otherwise, fold the eyecups forwards.Setting the eye distance1. Look into the eyepieces.2. Hold the eyepieces with both hands. Pushthe eyepieces together or separate themuntil you see a circular image. You may need a short time to become familiarwith using the instrument. Not to worry – aftera little while, it will become automatic.Leica E Series User ManualLeica EZ4, Leica EZ4 E or Leica EZ4 W 26

Switching on the LED IlluminatorAutomatic shutoffIf the illumination is not operated for 60minutes, it switches off automatically.The illumination can be reactivated by pressinga button on the membrane keypad.LED illumination1. Position a specimen in the middle of thebaseplate.Leica E Series User Manual2. Switch the two LED illuminators on or off asdesired:OOUse incident light for plastic, opaqueobjectsOOUse transmitted light for transmitted lightspecimens or transparent objects.OOUse the combination of both LED illuminators for partially transparent objects.For a detailed description of the illuminator, refer to page 29.Leica EZ4, Leica EZ4 E or Leica EZ4 W 27

Controlling the Automatic ShutoffAutomatic shutoffThe factory settings of the EZ4 modelsswitch the instrument off automaticallyafter one hour without user interaction. Thisautomatic shutoff helps to conserve the lightemitting diodes and save energy.Leica E Series User ManualDisabling the automatic shutoffTo disable the automatic shutoff, follow thesesteps:1. Switch the microscope off.Enabling the automatic shutoffTo re-enable the automatic shutoff, followthese steps:1. Switch the microscope off.2. Press and hold theandbuttons onthe light control unit simultaneously whileyou switch on the microscope.2. Press and hold theandbuttons onthe light control unit simultaneously whileyou switch on the microscope.The LEDs flash twice slowly and then remainswitched on. The automatic shutoff is nowdisabled.The LEDs flash three times slowly and thenremain switched on. The automatic shutoff isnow re-enabled.Leica EZ4, Leica EZ4 E or Leica EZ4 W 28

Illumination TypesThree-level incident light illuminationThe incident light illumination is madeup of 5 LEDs. Two, three or five diodes areswitched on depending on the level. Accordingly, the angle of incidence of the light beamon the object is switched from high to low.Press 1 Five diodes: greatestbrightness for incidentlight objectsLeica E Series User ManualRegulating brightnessThe brightness is adjusted using thekeys and affects all switched-on LEDs.Press 2 Top three diodes: shadowless uniform lighting forobjects with highly unevensurfacesPress 3 Two diodes: side lightemphasizes structures andincreases the contrastandPress 4 Incident light offPress 1 Switch on transmitted lightfor transparent objectsand transmitted lightspecimensLeica EZ4, Leica EZ4 E or Leica EZ4 W 29

FocusingFocusing raises and lowers the stereomicroscope using the focusing drive. Thespecimen is brought into sharp focus as soon asit is in the focal point of the objective.Focusing1. Set the magnification changer to the lowestlevel (8).3. Set the magnification changer to thesecond level (35).4. Use the focusing drive once again for finefocus.The focusing drive can be operated either leftor right-handed.After you have adjusted the image sharpness at high magnification, it remainsconstant even if you switch magnification levels(parfocal).2. Set the working distance to approx.100 mm for coarse focusing.Focusing drive torqueLeica E Series User ManualLeica EZ4, Leica EZ4 E or Leica EZ4 W 30

Magnification DisplayYou can read the configured magnification on the right-hand rotary knob of theLeica EZ4 models. The additional magnificationprovided by the eyepieces is already incorporated in the scaleLeica EZ4 models with open tubesOn the Leica EZ4 model withouteyepieces, only the magnification factorsof the magnification changer are indicated: 0.8,1, 1.25, 1.6, 2, 2.5, 3, 3.5.Different total magnifications are possible depending on which eyepieces areused with the Leica EZ4. The magnificationof the eyepieces is indicated on their outersurfaces, e.g. 10 /20, 16 /16 or 20 /12.Leica E Series User ManualLeica EZ4, Leica EZ4 E or Leica EZ4 W 31

Adjusting the Resistance of the Focus DriveAdjusting the resistanceIs the focusing drive too easy or too difficultto adjust? No problem – the resistance canbe adjusted individually depending on yourpersonal preferences:1. Grip the drive knobs with both hands andturn them towards each other until thedesired resistance is reached during focusing.Leica E Series User ManualLeica EZ4, Leica EZ4 E or Leica EZ4 W 32

Eyepieces (Only for Leica EZ4)Leica E Series User ManualEyepieces (Only for Leica EZ4) 33

Changing Eyepieces (Only for Leica EZ4, Open Version)Changing eyepiecesVarious eyepieces are available for the LeicaEZ4, allowing the stereomicroscope to beadapted even better to the specific requirements.The following eyepieces are available for selection:OO Eyepieces replaceable, fixed or adjustable:10 /20, 16 /16 or 20 /12OOEyepieces for eyeglass wearers 10 /20Band 16 /15B, fixed and adjustableAdjustable eyepieces for eyeglasses with straighteyecupsThe interchangeable eyepieces also offer thefollowing advantages:OOThe dioptric range is adjustable from 5 to –5OOMeasurement graticules can be used, seepage 38 and the following.Leica E Series User ManualEyepieces (Only for Leica EZ4) 34

Eyepieces and Eyecups (Only for Leica EZ4, Open Version)EyecupsThe eyecups can be replaced.Risk of infectionDirect contact with eyepieces is a potential transmission method for bacterialand viral infections of the eye. The risk can beminimized by the use of individual eyecups.Eyeglass eyepiece with adjustable diopter and straighteyecupsLeica E Series User ManualEyepiece for users who do notwear eyeglasses with adjustablediopter and inclined eyecupFixed eyepiece with inclinedeyecupEyepieces (Only for Leica EZ4) 35

Dioptric Correction (Only for Leica EZ4, Open Version)Dioptric correctionThe adjustable eyepieces for the LeicaEZ4 are also available with built-in dioptric correction, allowing the microscope to beused without glasses even by those with visionproblems. The correction comprises a maximum of 5 diopter settings.Users with normal vision do not need adjustable eyepieces. An adjustable eyepiece is sufficient for mild shortsightedness.Adjusting1. Set the dioptric correction for botheyepieces to "0".If vision is extremely poor or when graticulesare used, two adjustable eyepieces are needed.2. Select the lowest magnification and focuson a flat specimen.When the diopters are regulated exactly, thesharpness is constant over the entire magnification range ( parfocal).3. Select the highest magnification and readjust the sharpness.Once the diopters have been set, the proceduredoes not have to be repeated. Simply restoreyour diopter values on the eyepiece beforebeginning work with the stereomicroscope4. Select the lowest magnification again, butdo not look into the eyepieces.5. Carry out steps 6 to 8 for each objectiveindividually.6. Rotate the eyepiece counterclockwisein the " " direction as far as it will go( 5 diopter settings).Leica E Series User ManualEyepieces (Only for Leica EZ4) 36

Dioptric Correction (Continued)7. Look into the eyepiece.8. Slowly rotate the eyepiece individuallyin the "–" direction until the eye sees theobject sharply imaged.9. Select the highest magnification andrefocus if necessary.Now, if you adjust the magnification from thehighest to the lowest level, the specimen isalways brought into sharp focus. If not, repeatthe process.Leica E Series User ManualEyepieces (Only for Leica EZ4) 37

Graticules (Only for Leica EZ4, Open Version)GraticulesThe transparent graticules can beinserted in the adjustable eyepieces ofthe Leica EZ4. They enable easy measurementsat the specimen.Available eyepiece insertsGraticules10 mm/ 0.1 mm5 mm/ 0.1 mm5 mm/ 0.05 mm100 Div / 0.002"For equipment with a graticule in oneeyepiece, two adjustable eyepiecesare needed. Since one eye is focussed on theeyepiece with the graticule, it needs a second,adjustable eyepiece so that it can be alignedoptically with the first.Leica E Series User Manual100 Div / 0.001"150 Div / 0.0005"Stage micrometers50 mm (0.1 / 0.01 mm graduation)1" (0.001" graduation)CrosshairsCrosshairsEyepieces (Only for Leica EZ4) 38

Graticules: PreparationCare instructionsThe graticule must always be clean anddust-free! Never touch the graticule withbare hands! Always hold the graticule by theedges!Leica E Series User ManualVariant 1: 10 and 20 eyepiecesThe 10 and 20 adjustable eyepieces includean insert at the bottom with a snap mount.Variant 2: 16 eyepiecesOn the 16 adjustable eyepieces, the insert isfixed to the eyepiece by a screwed connection.1. Gently press the two small tabs on the snapmount together and pull out the insert.1. Screw the insert out counterclockwise.Eyepieces (Only for Leica EZ4) 39

Inserting the GraticulesInserting the graticules is the same for alleyepiece types.4. Put the insert back into the eyepiece.Depending on the variant, you have toeither press or twist the insert into place.6. Align the graticules by turning the eyepiecein the tube.1. Place the insert with the knurled side onthe work surface.2. Grasp the graticule so that you see the scalein laterally correct form.3. Carefully take hold of the edge of the graticule and push it sideways into the holderin the insert. Make sure that both plasticholders snap into place.Leica E Series User Manual5. Insert the eyepiece into the tube.Eyepieces (Only for Leica EZ4) 40

Photography Using the Leica EZ4 Eor Leica EZ4 WLeica E Series User ManualPhotography Using the Leica EZ4 E or Leica EZ4 W 41

IntroductionThank you for purchasing the Leica EZ4 E orLeica EZ4 W by Leica Microsystems. The specialdesign of the Leica EZ4 E or EZ4 W makes it auniversal, highly versatile tool for viewingmicroscopic specimens and capturing stillimages or video.Leica E Series User ManualPhotography Using the Leica EZ4 E or Leica EZ4 W 42

The BasicsYour Leica HD camera has many possibilities for various applications and conditions of use. Below are some basic concepts tohelp you get the most performance for yourapplication.Modes of use1. SD modeOO capture onto SD cardOOuse remote control2. WiFi mode (only available for EZ4 W)OO Wireless connection to wireless device (tablet,phone, PC)521433. USB modeOO Cabled connection to PC4. Ethernet modeOO Cabled connection to external network5. Capture buttonOO Captures image onto the SD cardLeica E Series User ManualNote direct connection to an HD displayis possible in all modes. Aspect ratio is16:9 except in USB mode where the aspect ratiois 4:3.Photography Using the Leica EZ4 E or Leica EZ4 W 43

Leica EZ4 E or Leica EZ4 W: Overview212431431. LED status indicatorOOBlinking red means starting upOOGreen means ready2. Capture button for SD card capture3. Mode selection panel4. SD card slotLeica E Series User ManualPhotography Using the Leica EZ4 E or Leica EZ4 W 44

Leica EZ4 E or Leica EZ4 W: Overview (Continued)1. HDMI for connecting the provided HDMIcable2. Ethernet socket for connecting an ethernetcable (cable not provided)3. USB for connecting the provided USB cable123FrontInfrared window: Receives the signal from theoptional remote control.Leica E Series User ManualPhotography Using the Leica EZ4 E or Leica EZ4 W 45

UnpackingPlease do not discard any of the packingmaterials. They should be used for safelystoring and transporting the instrument shouldthe need arise.The contents include CD with imaging software,HDMI cable, and USB cable.Leica E Series User ManualIf you ordered the handheld remote, you receivedthe remote with batteries.Photography Using the Leica EZ4 E or Leica EZ4 W 46

Get Set!Leica E Series User ManualGet Set! 47

USB Mode – Capturing and Adjusting Images Using a Computer10 eyepieces have a fixed circular fieldo

Leica EZ4, Leica EZ4 E or Leica EZ4 W 22 Eyepieces (only for Leica EZ4) 33 Photography Using the Leica EZ4 E or Leica EZ4 W 41 Get Set! 47 The Camera Remote Control (Optional) 55 Care, Transport, Contact Persons 68 Specifications 70 Dimensions 72