Purifier 100 / 10 - Unibz

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ÄKTA system Training GuidePurifier 100 / 10hamam ersenbioscihamamerscencebioscieamersham bioscienceamershambi osci ence

rConductivitypHOutlet ValveUV DetectorMixerPumpsPump APump BBuffer switchingvalve

ÄKTApurifier 10 / 100In-linefilter1 7623 4 5Injectionvalve (V1)UV3 H 4765Outletvalve (V4)ÄKTApurifier Sample Pump Sample ValveIn-linefilterInjectionvalve (V1)1 7623 45MixerUV3 psApHBSamplepumppH detectorFractioncollectorFrac-9501 87263451 8726345Sample selectionvalve (V5)Outletvalve (V4)

ÄKTApurifier 100 AirIn-linefilter1 7623 4 5Injectionvalve (V1)UV3 H ensor81234765Outletvalve (V4)ÄKTApurifier Buffer ValveIn-linefilter1 7623 4 5Injectionvalve (V1)UV3 H 1 8726345Bufferselectvalve (V6)4765Outletvalve (V4)

ÄKTApurifier 10 XTIn-linefilter1 7623 4 5Injectionvalve (V1)UV3 H 4765Outletvalve (V4)

PUMP P-900MixerPump APump BProportionValveA2Acid /AlkaliÄKTA 10 mls/minÄKTA 100 mls/minA1 B2BufferB1SaltGRADIENT 6 mls/min6 -14 mls/min 30 mls/min30 - 50 mls/min50 - 100 mls/minWaterMIXER0.6 ml mixer2 ml mixer2 mls mixer5 mls mixer12 mls mixer

PUMP P-900 InletsMixerPump BPump ASwitchingValvesBuffer SelectValve IV-908A2A123A11A12A131 84A14B1Water756A16A15B2A17A18Salt

PUMP P-900 Manually Priming Buffer Inlet LinesPump ACommon inletA2Pump BA1Common inletManualFlowpathManualA1 or A2BufferValveA B1 or B2B1B2FlowpathBufferValveBOpenDraw Airout ofBufferLine

PUMP P-900 Back - Wash LinesPump Back-WashLinesSyringe fittingWash OUTIN-LINEone-wayvalveBufferOUTBall outletcheck valveHigh pressurePiston sealPiston20% Ethanol : WaterBackwash pumpdiaphragmBall inletcheck valveWashINBufferIN

ON-LINE FILTERSP-903 ( 10 ml/min Pump )Low Flow HPLC FilterP-901 ( 100 ml/min Pump )High Flow Fibre FilterPolymer SupportLOAD (sample)INJECT lumnColumnColumn1LOAD (wash loop)Waste5SampleWaste

INJECTION VALVE INV- asteWASTE

SAMPLE LOADING METHOD1COLUMN2LOADSAMPLE7PUMP3654Needle PortWasteWasteAlways tighten by handwith needle insertedDo Not Overtighten1COLUMNLOADSAMPLE72PUMP3645Luer LockAdapterWasteWasteSuperloop

SUPERLOOPS10 ml50 mlBuffer150 USHWASTEPUMP orSYRINGECOLUMNCOLUMNSampleEtchedGrooveVolumeMax. allowed columnpressureCode No.10 ml50 ml150 ml4 Mpa4 Mpa2 Mpa18-1113-8118-1113-8218-1023-85

INV-907 Injection Valve - Injection PortPEEK Valve BodyInjection Needle PortValve SealFerruleNeedleTubeNeedleNeedleAdapter

INV-907 Injection Valve - Luer e

Fluid DynamicsFlowPEEK TubingHigh Resistanceto FlowLow Resistanceto FlowEndBeginning

Fluid DynamicsFlow - Injecting a LoopThe way we imagine it FlowsThe way it Actually FlowsBubble Segmentation100ul LoopWaste50ul Load60ul Load100ul Load200ul Load Partial Loading Loop No more than 1/2 Loop Volume Total Loading Loop Inject 2X Loop Volume

LOADING A SAMPLE LOOPInjection -950Pump65Waste

LOADING A SUPER LOOPInjection eWasteSample

MULTI-LOADING A SAMPLE LOOPInjection /O4576WashSamplesWaste

MULTI-LOADING A SUPER LOOPColumn12PumpLoadInjectInjection S3 S4 S5 S6S8

DIRECT LOADING ON TO COLUMNColumn1Pump723InjectLoad645Extra Tube1/16” or 1/8”TubingWasteSample

REVERSE FLOW VALVE ( INV-907 )172Pump354ColumnExtra Tube6StopPlugMONITORDownward FlowPosition 1172Pump34ColumnExtra Tube56StopPlugMONITORUpward FlowPosition 2

OUTLET VALVE PV-908DETECTORTo F8F4F3F5F6

Fraction UDNUOBStartInject500250%B0A1 A2 A3 .0

HiTrap Connections1/16” Female1/16” Male18-1112-551/16” Fingertight Fitting1/16” FemaleM6 Male18-1112-57M6 male / 1/16” FemalePhenyl SepharoseHiTrap ColumnM6 MaleM6 Female18-1112-58M6 female / 1/16” maleFlow Cell Inlet1/16” Male

Multiple HiTrap Arrangment ( Series )Phenyl SepharosePhenyl SepharosePhenyl SepharosePhenyl SepharosePhenyl SepharosePhenyl SepharoseDeortctet orcetDeortcteDe

Traditional FPLC fitting to ÄKTA / HPLCInjector1/16” Fingertight Fitting18-1112-55100u1/16” FEMALE / M6 FEMALE18-1123-94lFlange M6 Fitting1/16” MALE / M6 FEMALE18-1112-58Flow Cell

Tubing Fittings18-1112-551/16” Fingertight Fitting1/16” PEEKTMTubing18-1112-58Flange Fitting AdapterFPLC Flange Fitting18-1112-51Luer Fitting Adapter18-1112-52Fingertight Stop PlugFlangless Inlet 8-1127-0718-1112-57M6 male / 1/16” FemaleLuer Syringe18-1112-505/16” Stop PlugFlangless Buffer ��18-1127-0618-1017-98Tubing ConnectorM6 male / O - ring18-3955-011/16”female X 1/16”female Union TiUnion 2 Fingertight Fittings18-1123-94M6 female X 1/16”female UnionFPLC Flange - 1/16” Fingertight

COMPRESSION FITTINGSUntightenedCorrectly tightenedObstructedOver tightened Tubing Restricted

PEEK TubingTMGray, 1/16”x 1.0 mm ( 7.85ul / cm )Green, 1/16”x 0.75 mm ( 4.42ul / cm )Orange, 1/16”x 0.5 mm ( 1.96ul / cm )Blue, 1/16”x 0.25 mm ( 0.49ul / cm )Teflon / Tefzel Tubing3/16” OD18-1112-471/8” OD18-1121-161/16” OD18-1119-74

Tubing Kit Changes - ÄKTApurifier 10GENERAL FLOW (at delivery)G5100ulInjection valve V1G9Outlet Valve V4 (7ul)Accumulator(40ul)UV Flow cellG8G60.25 mm i.dBlue PEEKG5 400mmG6 160mmG7 80mmG8 130mmG9 500mmG7ConductivityFlow cell (14ul)FR-904(0.2Mpa, 5ul)NB: Never usethe FR-904 withthe pH electrodepH Flow cell (100ul)Tubing KitAKTApurifier 10 , 0.50 tubing kitAKTApurifier 10 , 0.50 tubing kit pH electrodeAKTApurifier 10 , 0.25 tubing kitNo FracFrac 90123235488Frac 950382504238

Tubing Kit Changes - ÄKTApurifier 10HIGH FLOWG5100ulInjection valve V1H9Outlet Valve V4 (7ul)Accumulator(40ul)UV Flow cellH8H6H7ConductivityFlow cell (14ul)0.50 mm i.dOrange PEEKH6 160mmH7 80mmH8 130mmH9 500mmFR-904(0.2Mpa, 5ul)NB: Never usethe FR-904 withthe pH electrodepH Flow cell (100ul)Tubing KitAKTApurifier 10 , 0.50 tubing kitAKTApurifier 10 , 0.50 tubing kit pH electrodeAKTApurifier 10 , 0.25 tubing kitNo FracFrac 90123235488Frac 950382504238

Tubing Kit Changes - ÄKTApurifier 100HIGH FLOW (at delivery)G5100ulInjection valve V1G9Outlet Valve V4 (7ul)Accumulator(40ul)UV Flow cellG8G60.75 mm i.dGreen PEEKG6G7G8G9G7ConductivityFlow cell (14ul)FR-904(0.2Mpa, 5ul)160mm80mm130mm500mmNB: Never usethe FR-904 withthe pH electrodepH Flow cell (100ul)Tubing KitAKTApurifier 100 , 0.50 tubing kitAKTApurifier 100 , 0.75 tubing kitAKTApurifier 100 , 1.00 tubing kitAKTApurifier 100 , 0.50 tubing kit pH electrodeAKTApurifier 100 , 0.75 tubing kit pH electrodeAKTApurifier 100 , 1.00 tubing kit pH electrodeAKTAbasic 100 , 0.75 tubingNo Frac290Frac 901232470769354672955Frac 950382620809504862995

Tubing Kit Changes - ÄKTApurifier 100LOW FLOWL5100ulInjection valve V1L9Outlet Valve V4 (7ul)Accumulator(40ul)UV Flow cellL8L60.50 mm i.dOrange PEEKL6L7L8L9L7ConductivityFlow cell (14ul)160mm80mm130mm500mmFR-904(0.2Mpa, 5ul)NB: Never usethe FR-904 withthe pH electrodepH Flow cell (100ul)Tubing rAKTApurifierAKTApurifierNo 00tubingtubingtubingtubingtubingtubingkitkitkitkit pH electrodekit pH electrodekit pH electrodeFrac 901232470769354672955Frac 950382620809504862995

A-900 465WasteWasteCOLUMNPUMPLOAD

Needle HeightStandard VialStandard Vial Needle Height Insertvolume left2mm450ul1mm350ul0mm200ul3mm12ul

A-900Sample Injection ( Flushed Loop Injection olumnSample hethevalvevalveflusheschannel.channel.Min.( (30ul30ul) )withwithMin.AirSegmentSegmentAirMin.( (35ul35ul) sthanthantwicetwice( (noloopvolumevolumelooprecommended) ortfluid

A-900Sample Injection ( Partial Loop Injection Min.( (30ul30ul) )withwithMin.AirSegmentSegmentAirMin.( (35ul35ul) olumnSample needlepumppumpcolumnSample anhalfhalf( (noloopvolumevolumelooprecommended) )recommendedcolumncolumnSample id

A-900Sample Injection ( ul Pick-up Injection mple needleSample ReservoirpumpcolumnSample ortfluid

System Compensation VolumeEquilibration PhaseABCOLUMN TOP0%ABCOLUMN TOP20%Effect8 mlsCommand20%B0%B8mls

Gradient Delay VolumeGradient PhaseABCOLUMN TOP0%ABCOLUMN TOP100%Effect16 mls100%BGradient DelayCommand 100%

Column Pressure ( compensation ) Flow RestrictorColumn PressuresColumn Hardware Pressure LimitsColumn Shatters DANGEROUSColumn Matrix Pressure LimitsMatrix Collapses .0MPaMPa0.0Pre-column pressure 0 MPaPumpPump0.3MpaMpa0.30.3 MPaGELPressPressDropDropGEL0.3MPaMPa0.3(0.3- -0.0)0.0)(0.30.0 MPa0.0MPaMPa0.00.5ReadingReading0.5Pre-column pressure 0 MPaGel Top PressurePumpPump0.3MpaMpa0.30.3 DropGELColumnPressPressDropDropColumn(0.3 0.0)0.0)(0.3(0.3- -0.2)0.2)(0.3(0.5 0.2)0.2)(0.50.3MPaMPa0.3Gel Bottom Pressure0.1MPaMPa0.10.2 Pa0.5

AKTApurifier 100 , 0.50 tubing kit 232 382 AKTApurifier 100 , 0.75 tubing kit 470 620 AKTApurifier 100 , 1.00 tubing kit 769 809 AKTApurifier 100 , 0.50 tubing kit pH electrode 354 504 AKTApurifier 100 , 0.75 tubing kit pH electrode 672 862 AKTApurifier 100 , 1.00 tubing kit pH electrode 955 995 AKTAbasic 100 , 0.75 tubing 290

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Figure 1. Black box model of the solar water purifier Energy flow: Solar water purifier will receive and collect solar energy converts this energy into heat. Material flow: Dirty and impure water is given as input in water purifier and clean drinking water comes out as output. Information Flow: From electrical energy of solar circuit and battery, controller shows charging status of

4. Clean any remaining dust or hair inside the purifier using a vacuum hose. DO NOT use water or liquids to clean the purifier. 1. Plug in the air purifier and turn it on. 5. Remove the new filter from its packaging and place it into the purifier with the pull tabs facing the opening. The HEPA filter should be facing the opening.

Power Power on/off the air purifier Timer Set the timer of the air purifier Mode You can choose Smart or Sleepmode Fan speed You can choose fan speed Light You can turn on/off the air purifier lights Schedule You can set the Air Purifier operating schedule Notification You can receiveair quality status, filter remaining information Manage product

Packaging, Know Your Air Purifier P.6 Features P.7 Operation and Use, Replacing HEPA Filter P.8 Replacing UV Light, Maintenance P.9 Troubleshooting, Service Center P.10 Limited Warranty P.11 Warranty Card P.13 Order Form P.14 AIR PURIFIER MODELS This instruction manual applies to the following Crane Air Purifier product range: PENGUIN