ABBOTT CELL-DYN 11800 SYSTEM
mck.coABBOTTCELL-DYN 11800 SYSTEM StockCheQuick Reference GuideList No. 07H92-01
FOREWORDmFOREWORD2The CELL-DYN 1800 System is manufactured by Abbott Diagnostics, Abbott Laboratories, 2000 Abbott Park Road, AbbottPark, IL 60064, USA. Please direct all inquiries concerning information in this manual to the foregoing address.Content Revised, Added, or DeletedFirst release– April 2004ck.coRevision07H92-01Any product information in this document should be used in conjunction with the latest version of the Operations Manual. If anydiscrepancies in information exist within this document or any others, the latest version of the Operations Manual takesprecedence.All samples (printouts, graphics, displays screens, etc.) are for information and illustration purposes only and shall not be usedfor clinical or maintenance evaluations.Data shown in sample printouts and screens do not reflect actual patient names or test results.ockCheNo part of this media may be reproduced, stored, retrieved, or transmitted in any form or by any means without the prior writtenpermission of Abbott Laboratories.All Abbott Laboratories product names and trademarks are owned by and licensed to Abbott Laboratories, its subsidiaries oraffiliates. No use of any Abbott trademark, trade name, trade dress, or product name may be made without the prior writtenauthorization of Abbott Laboratories, except to identify the product or services or Abbott Laboratories. All other trademarks,brands, product names, and trade names are the property of their respective companies. All rights reserved.Except as permitted above, no license or right, express or implied, is granted to any person under any patent, trademark, orother proprietary right of Abbott Laboratories.TrademarkCELL-DYN Trademark StatementCELL-DYN is a registered trademark of Abbott Laboratories 2004, Abbott Laboratories, Abbott Park, IllinoisStCELL-DYN 1800 System Quick Reference Guide9140393A April 2004
INTRODUCTIONmCELL-DYN 1800 System Quick Reference Guide9140393A April 2004PC Keyboard (Keys)In some cases, the Operator must utilize the keys on the PCkeyboard. Pressing the F1 through F8 function keys will initiateThe CELL-DYN 1800 System Quick Reference Guide containsthe action specified by a corresponding screen label. Theprocedures used for CELL-DYN 1800 systems.alphanumeric keys (including punctuation symbols) may beused to enter specimen identification in a data entry field.The Reference Guide is designed to be folded so that theprocedures will be visible while operating the CELL-DYN 1800. Additional function keys such as the [ ] Enter key and theThe Reference Guide can be placed so that you can view two [ESC] key may be utilized as well. Special function keys, suchas the arrow keys, may appear as a symbol substituted for thepages at a time.word. Instructions for special function keys will read; forAll procedures in this Reference Guide were designed asexample “Press the [ ] arrow key.”step-by-step procedures. You should follow these proceduresThe Print Screen key on the PC keyboard can be used to printin the order they are presented.the screen as it is displayed on the LCD. This allows theText Conventions Used in this Operator’s Reference GuideOperator an option to print the screen when the [PRINT] key isIn this Reference Guide, procedural instructions are explained not available.in logical groups, using numbered steps. Illustrations andNOTE: Press the Print Screen key only when the screen is atdrawings appear where they are useful to the explanation.a static state. Pressing the key during an instrumentText conventions are as follows:action (e.g., Run cycle) may not print the screenproperly.Menu NameockCheck.coUsing this Quick Reference GuideInstrument StatusThe menu name is shown in bold, uppercase, sans serifletters; for example, SETUP. The word “menu” is notInstrument status is displayed in uppercase, regular letters; forcapitalized, although it is referenced in this manual, and it doesexample READY.not appear on the display screen.Softkeys (Screen Label Keys)Screen MessagesStScreen messages or other screen displays will appear in bold,Directly below the screen is a row of eight unlabeled,pressure-sensitive softkeys that correspond to screen labels or Courier letters, for example, Waste Full.menu options found on the lower segment of the displayscreen. Pressing one of these softkeys initiates the actionspecified by a corresponding screen label. Screen labels areshown in bold, uppercase, sans serif letters enclosed inbrackets; for example [QUALITY CONTROL].INTRODUCTION3
INTRODUCTIONBefore You Call Us for Customer SupportmCUSTOMER SUPPORT4ockCheck.coIf you need information or help in diagnosing a problem,Please have the following information ready:technical assistance is available by telephone. In the U.S., thisservice is available 24 hours a day, seven days a week by1. Customer Account numbercalling Abbott Diagnostics Customer Service at:2. Instrument Model (CELL-DYN 1800)1-877-4ABBOTT (1-877-422-2688).3. CELL-DYN Serial NumberFor customer support in Canada, call: 1-800-387-83784. Software version in use5. Lot Numbers being used for:For customer support outside the U.S. and Canada, call yourlocal Hematology Customer Support representative.a. ReagentsFor correspondence, the address in the U.S. is:b. Calibratorsc. ControlsAbbott Diagnostics Division6. Test results printoutsCustomer Service200 Abbott Park Road7. Quality Control Log:Abbott Park, IL 60064, U.S.A.a. Most recent calibration informationb. Daily control Values8. Maintenance Log information9. Printed fault Log for Error Messages and Flags relating tothe problemStCELL-DYN 1800 System Quick Reference Guide9140393A April 2004
mCELL-DYN 1800 System Quick Reference Guide9140393A April 2004The biohazard icon identifies an activity or areawhere personnel may be exposed to infectioussubstances if procedural or engineering controls arenot observed.SAFETY PRECAUTIONSck.coOperation, maintenance, and servicing of hematology systemsmay expose individuals to potential safety and health hazards.All work must be performed in accordance with proceduresdescribed in the CELL-DYN 1800 System Operator’s Manualor as directed by an Abbott Representative.Warning - Potential Biohazard: Consider all clinicalspecimens, controls, surfaces, or components thatcontain or have contacted blood, serum, or bodilyfluids as potentially infectious. Wear gloves, labcoats, and safety glasses, and follow other biosafetypractices as specified in the OSHA BloodbornePathogen Rule (29 CFR, Part 1910.1030) or otherequivalent biosafety procedures.Warnings are inserted in this Reference Guide to alertpersonnel to potential hazards.The standard warningconventions including signal words (e.g., CAUTION) and iconsare described below.Signal WordsDenotes a hazard which, if not avoided, could result in moderate to serious injury.CAUTION:Denotes a potential hazard that could result in minor injury.Also used for conditions or activities that could threatenequipment or performance.NOTE:For detailed safety information, refer to the CELL-DYN 1800System Operator’s Manual.ockCheWARNING:Denotes special operator information or standard practices.Warning IconsThe general hazard icon identifies an activity or areathat may present a hazard to personnel orequipment.StThe electrical hazard icon alerts personnel to thepossibility of electrical shock if procedural orengineering controls are not observed.INTRODUCTION5
ockCheck.coNOTES6mINTRODUCTIONStCELL-DYN 1800 System Quick Reference Guide9140393A April 2004
MASTER TABLE OF CONTENTS7mMASTER TABLE OF CONTENTS4 - Quality Control Setup1.1 Daily Startup . 1-24.1 Entering Means & Limits (Manual Method) .4-21.2 Background Counts . 1-54.2 Entering Means & Limits (by Upload from Disk).4-51.2.1 Background Counts. 1-51.3 Quality Control . 1-71.3.1 Performing Quality Control Runs . 1-72 - Specimen Processing2.1 Specimen Processing . 2-23 - Maintenance3.1 Maintenance Log . 3-25 - Calibration5.1 Pre-Calibration Procedures .5-25.1.1 Pre-Calibration Checklist .5-25.1.2 Precision Test.5-35.1.3 Calibration Verification.5-75.2 Auto Calibration.5-126 - Troubleshooting Guide6 Troubleshooting.6-2ockChe3.2 Error Message Logsheet. 3-4ck.co1 - Daily Procedures3.3 Auto Clean . 3-63.3.1 Auto Clean Procedure . 3-63.4 Special Procedures . 3-93.4.1 CELL-DYN 1800 Aperture Plates Cleaning Procedure . 3-103.4.2 CELL-DYN 1800 Supplemental ApertureCleaning Procedure. 3-147 - Parts and Accessories List7.1 CELL-DYN Equipment, Parts, and Accessories.7-27.2 CELL-DYN 1700/1800 Accessory Kit (List No. 03H54-01) .7-47.3 CELL-DYN 1800 Reagent Line Kit (List No. 91072-01) .7-57.4 CELL-DYN Reagents .7-63.4.3 “Y” Fitting Cleaning Procedure . 3-177.5 CELL-DYN Controls and Calibrators .7-73.4.4 CELL-DYN 1800 Hemoglobin Flow CellCleaning Procedure. 3-207.6 CELL-DYN Consumables.7-8StCELL-DYN 1800 System Quick Reference Guide9140393A April 2004
ockCheck.coNOTES8mMASTER TABLE OF CONTENTSStCELL-DYN 1800 System Quick Reference Guide9140393A April 2004
mCELL-DYN 1800 System Quick Reference Guide9140393A April 20041 - Daily Procedures1.1 Daily Startup . 1-21.2 Background Counts . 1-51.2.1 Background Counts. 1-51.3 Quality Control . 1-7StockChe1.3.1 Performing Quality Control Runs . 1-71ck.coTABLE OF CONTENTS1 - Daily Procedures21-1
1.1 Daily StartupRemove controls from refrigerator and allowto warm to room temperature for 15 minutes.Follow mixing and handling steps outlined oncontrol assay sheet or CELL-DYN Calibrator andControl Mixing and Handling Instructions.1If Standby or Initialized is displayed in the MAINmenu status box, press [PRIME/RUN] to bring theinstrument to the Ready State.CELL-DYN 16 Control or CELL-DYN 22 ControlNecessary equipment: Protective equipment (gloves, lab coat andeye protection) Printer paper CELL-DYN 16 or CELL-DYN 22 Controls Reagents Maintenance logDiluent (20L)Diluent (4x3.8L)Detergent (20L)Detergent (4x3.8L)3Check printer paper is installed and feedingcorrectly.ockCheCELL-DYN Reagents:CN-Free Diff Lyse (3.8L)CN-Free Diff Lyse (960mL)StCELL-DYN 1800 System Quick Reference Guide2ck.coPerform these tasks daily and document inthe Maintenance Log.m1.1 Daily Startup1-2NOTE:4The CELL-DYN 1800 is compatible withprinters that support ESC-P or PrinterControl Language Release 3 (PCL-3).Any printer offering ESC-P or PCL-3compatibility may be used to printreports generated by this instrument. Forinformation on ordering printers, refer toAppendix A: Parts and Accessories inthe CELL-DYN 1800 System Operator’sManual.Check that the Diluent tubing is properlyplaced into reagent container and volumelevel is sufficient.5R017a9140393A April 2004
mCELL-DYN 1800 System Quick Reference Guide6Check that the Detergent tubing is properlyplaced into reagent container and volumelevel is sufficient.7Run Background counts until acceptableresults are obtained for all parameters (WBCRBC, HGB and PLT). For detailedinstructions, see “1.2.1 Background Counts” onck.coCheck that the Lyse tubing is properly placedinto reagent container and volume level issufficient.9140393A April 20048page 1-5.StRun a minimum of two levels of CELL-DYNcontrols. For detailed instructions, see “1.3.1Performing Quality Control Runs”.SpecificationsWBC 0.5 K/µLRBC 0.05 M/µLHGB 0.1 g/dLPLT 10.0 K/µLR017aockCheR017aParameter9Rerun controls, if any QC results are outsideallowable limits1.1 Daily Startup10Verify results are within acceptable limitsbefore running any patient specimens.111-3
12ockChe1513StCELL-DYN 1800 System Quick Reference Guide14ck.coYou are now ready to run patient specimens.1-4m1.1 Daily Startup9140393A April 2004
mCELL-DYN 1800 System Quick Reference Guide12From MAIN menu, press [RUN].ockCheBackground counts are routinely run toensure sample analysis accuracy and propersystem performance. The background count checkmay help in ruling out issues with fluidics or valveoperation.1.2.1 Background CountsStFrom SPECIMEN TYPE menu, press[NORMAL BACKGRND].31From RUN menu press [SPECIMENTYPE].2ck.co1.2 Background Counts9140393A April 2004When the following screen appears, pressthe touch plate to initiate background run.1.2 Background Counts4Background count results are displayedwhen the run cycle is complete.5Compare the background results to the followingacceptable ranges for these parameters:ParameterSpecificationsWBC 0.5 K/µLRBC 0.05 M/µLHGB 0.1 g/dLPLT 10.0 K/µL1-5
6ockChe9If results are still unacceptable, refer toSection 6: Troubleshooting Guide.StCELL-DYN 1800 System Quick Reference Guide7ck.coIf background counts do not fall within theacceptable limits, repeat the backgroundcount procedure.1-6m1.2.1 Background CountsIf background counts do not fall within theacceptable limits, repeat the background countprocedure.108119140393A April 2004
Controls are used to determine whether aninstrument is operating with accuracy. Controlsconsist of stabilized blood cells with assayedranges for each measured parameter.9140393A April 200411.3.1 Performing Quality ControlRuns1If the System has been idle for fifteen minutes ormore, run a background prior to running anyCELL-DYN controls. Remove the CELL-DYNControls from the refrigerator and allow controls towarm at ambient room temperature for fifteenminutes. For operating instructions, refer toCELL-DYN 1800 System Operator’s Manual,Section 11: Quality Control, Subsection:Quality Control Menu, Performing a QC Run.CELL-DYN Controls2ck.co1.3 Quality ControlmCELL-DYN 1800 System Quick Reference GuideCELL-DYN controls provide three levels (low,normal and high) for each measured parameter.ockCheNecessary Equipment: Protective equipment (gloves, lab coat andeye protection) CELL-DYN 16 Controls or CELL-DYN 22ControlsStFrom MAIN menu, press [RUN].3From RUN menu, press [SPECIMENTYPE].1.3 Quality Control4From SPECIMEN TYPE menu, press [QCTYPE].51-7
1.3.1 Performing Quality Control RunsUse the [ ] and [ ] arrow keys on the PCkeyboard to highlight the file into which youwant to run your control.Verify that the Control level being runcorresponds to the file selected in Step 8.Follow the mixing and handling steps outlinedon the control assay sheet, or the CELL-DYNCalibrator and Control Mixing and HandlingInstructions.9Remove the cap from the pre-mixed controltube.ockCheStCELL-DYN 1800 System Quick Reference Guide7After the required file is highlighted, press[RETURN]. The file name will appear in theupper left corner of the RUN screen.m6ck.coFrom QC TYPE menu, press the level ofcontrol to be run.1-810NOTE:811Procedure continued onnext page.9140393A April 2004
mCELL-DYN 1800 System Quick Reference Guide11Press the touch plate to activate the run.12After the control specimen has beenaspirated from the tube, the probe moves upthrough the wash block. There will be anaudible beep and the message line displays: REMOVE SPECIMEN. 13ck.coPlace the open tube under the SampleAspiration Probe and raise the tube so thatthe end of the probe is deeply immersed inthe control material.9140393A April 2004ockCheRemove the tube and replace the cap.After the cycle is complete, the resultsdisplayed on the RUN screen willautomatically print if that option was selected.14Optionally, press [PRINT REPORT] for a printoutof the results before running the next specimen.Press [SPECIMEN TYPE], followed by [QCTYPE], to select the next control to be run.Repeat this procedure to run other levels ofcontrol in their respective files.1516When all controls are complete, verify controls arewithin limits.Values out of limits will be highlighted on screen orunderlined when printed.If any values are out of limits, repeat control. Ifcontrols continue to be out of range, call AbbottDiagnostics Customer Service for assistance.StIf controls are within limits, you can run patientspecimens.1.3.1 Performing Quality Control Runs1-9
1-10m1.3.1 Performing Quality Control RunsockCheck.co11StCELL-DYN 1800 System Quick Reference Guide9140393A April 2004
mCELL-DYN 1800 System Quick Reference Guide9140393A April 20042 - Specimen ProcessingStockChe2.1 Specimen Processing . 2-21ck.coTABLE OF CONTENTS2 - Specimen Processing22-1
2.1 Specimen Processing3Confirm specimen ID on the tube.ockCheSelect the desired specimen tube from therack.After specimen is collected, place it in a racknear the CELL-DYN 1800 System.StCELL-DYN 1800 System Quick Reference Guide1After daily startup is completed, go to MAINmenu.mNecessary equipment: Protective equipment (gloves, lab coat andeye protection) Anticoagulated (EDTA) whole blood specimenck.co2.1 Specimen Processing2-24From the MAIN menu, press RUN.259140393A April 2004
9140393A April 20046Using the PC keyboard, enter specimen IDinformation and demographics.7With the cap tightly secured, gently invert thetube 10 to 15 times.8ockCheck.coFrom the RUN menu, press [SPECIMENTYPE], then press [PATIENT SPECIMEN].mCELL-DYN 1800 System Quick Reference GuideStRemove the cap from the pre-mixedspecimen tube.9Place tube under sample aspiration probeand raise the tube so that the end of theprobe is deeply immersed in the specimen.2.1 Specimen Processing10Press touch plate to activate run.112-3
1215StCELL-DYN 1800 System Quick Reference Guide13Optionally, press [PRINT REPORT] for a printoutof the results before running the next specimen.ockCheWhen the READY message appears,continue specimen processing with anothertube if necessary.After the cycle is completed, the results willdisplay on the RUN screen and willautomatically print if that option was selected.14ck.coAfter the instrument aspirates the specimen,the probe moves up. There will be an audiblebeep and the message line displaysREMOVE SPECIMEN. Remove the specimen andreplace the cap.2-4m2.1 Specimen Processing169140393A April 2004
01 Jun 200412:45mRightCELL-DYN 1800 System Quick Reference Guide9140393A April 20043 - Maintenance3.1 Maintenance Log . 3-23.2 Error Message Logsheet. 3-43.3 Auto Clean . 3-63.3.1 Auto Clean Procedure . 3-63.4 Special Procedures . 3-93.4.1 CELL-DYN 1800 Aperture Plates Cleaning Procedure . 3-103.4.2 CELL-DYN 1800 Supplemental ApertureCleaning Procedure. 3-143.4.3 “Y” Fitting Cleaning Procedure . 3-17StockChe3.4.4 CELL-DYN 1800 Hemoglobin Flow CellCleaning Procedure. 3-201ck.coTABLE OF CONTENTS3 - Maintenance23-1
Left01 Jun 200412:453.1 Maintenance Logm3.1 Maintenance Log3-2ockCheck.coDocumentation of all maintenance performed is an important part of goodlaboratory practices. Maintenance procedures performed in a timely mannerwill assist in keeping instrument performing as expected.StCELL-DYN 1800 System Quick Reference Guide9140393A April 2004
01 Jun 200412:45mRightCELL-DYN 1800 System Quick Reference Guide9140393A April 2004CELL-DYN 1800 MAINTENANCE LOGDAYDAILYWEEKLYMONTHLYDaily StartupDaily ShutdownAuto CleanClean Aspiration Probe ExteriorRinse Lyse Inlet LinesRinse Reagent Inlet LinesSEMI-ANNUAL Clean PrinterAS-REQUIRED Clean HGB Flow CellClean Pre-Mixing CupEmpty Instrument WasteClean/Replace Aperture PlateClean/Replace Aspiration ProbeClean/Replace Aspiration Probe Wash 8293031ockCheClean/Replace SyringesYEAR1ck.coMONTHClean/Drain Vacuum AccumulatorClean Bar Code Scanner LensClean “Y” FittingSupplemental Aperture CleaningStPrepare Instrument for Extended Period ofNon-Use or Shipping3.1 Maintenance Log3-3
Left01 Jun 200412:453.2 Error Message LogsheetockCheck.coThe Error Message Log is used for documentation of problems seen duringdaily operations, such as fault messages, error messages and unexpecteddata obtained. Keeping a record of how the problem was resolved is a helpfulaid for future troubleshooting. A copy of the Error Message Logsheet has beenprovided for your reference. Refer to Appendix C – Sample Logs andWorksheets found in the CELL-DYN 1800 System Operator’s Manual foradditional templates.3-4m3.2 Error Message LogsheetStCELL-DYN 1800 System Quick Reference Guide9140393A April 2004
01 Jun 200412:45CELL-DYN 1800 System Quick Reference Guide9140393A April 2004ERROR MESSAGE LOGSHEETTIMESEQUENCE #OBSERVATIONRESOLUTIONStockCheTECHck.coInstrument Serial Number:DATEmRight3.2 Error Message Logsheet3-5
Left01 Jun 200412:453.3 Auto Clean3.3.1 Auto Clean ProcedureThe Auto Clean procedure uses anenzymatic cleaning solution to clean and drain thefluidics system and reduce protein buildup in linesand apertures.Materials needed: Protective equipment (gloves, lab coat andeye protection) CELL-DYN Enzymatic Cleaner Maintenance log Standard specimen tube (no anticoagulant)Dispense a portion (3/4 full) of the enzymaticcleaner into a clean standard specimen tube.Label with name and date.3From MAIN menu, press [SPECIALPROTOCOLS].CELL-DYN Enzymatic Cleanerck.co1m11ockChe3.3 Auto Clean3-64Press [AUTOCLEAN] to begin the AutoClean cycle.25Return bottle to refrigerator but allow thedispensed enzymatic cleaner to warm at roomtemperature.CAUTION:Enzymatic Cleaner is extremelyslippery. Wipe excess cleaner offtop of tube before inserting stopper.StCELL-DYN 1800 System Quick Reference Guide9140393A April 2004
01 Jun 200412:45mRightCELL-DYN 1800 System Quick Reference Guide6Press the [START CLEAN] soft key toaspirate the enzymatic cleaner. When theenzymatic cleaner has been drawn from thetube, the probe will move up through the washblock, and the REMOVE SPECIMEN messageappears on the display screen.When process is complete, press [MAIN] toreturn to MAIN menu.9StSave the remaining enzymatic cleaner in therefrigerator for up to one week. Discard enzymaticcleaner if it becomes cloudy or if original bottlereaches expiration date.During the cleaning process a message PROCESS ACTIVE appears on thescreen.8NOTE:The complete cycle takes approximatelyseven minutes.Do not press the touch plateockCheNOTE:7ck.coPlace the tube with enzymatic cleaner underthe aspiration probe. Raise the tube so thatthe end of the probe is deeply immersed inthe enzymatic cleaner.9140393A April 2004From the MAIN menu, press [Run] followedby [SPECIMEN TYPE] and [NORMALBACKGRND]. Run background counts untilacceptable results are obtained for all backgroundparameters.3.3.1 Auto Clean Procedure10Press [MAIN] to return to the MAIN menu.113-7
Left01 Jun 200412:4512ockChe31StCELL-DYN 1800 System Quick Reference Guide2ck.coRecord this maintenance in the MaintenanceLog3-8m3.3.1 Auto Clean Procedure49140393A April 2004
01 Jun 200412:45CELL-DYN 1800 System Quick Reference Guide56These procedures are performed as needed, when additional cleaning isrequired. See CELL-DYN 1800 System Operator’s Manual, Chapter 9, for acomplete list of cleaning procedures.TABLE OF CONTENTS3.4.1 CELL-DYN 1800 Aperture Plates Cleaning Procedure. 3-103.4.2 CELL-DYN 1800 Supplemental Aperture Cleaning Procedure. 3-143.4.3 “Y” Fitting Cleaning Procedure. 3-173.4.4 CELL-DYN 1800 Hemoglobin Flow Cell Cleaning Procedure . 3-20StockChe8ck.co3.4 Special ProceduresmRight3.4 Special Procedures979140393A April 2004103-9
Left01 Jun 200412:453.4.1 CELL-DYN 1800 Aperture Plates Cleaning ProcedureOn rare occasions debris buildup could clog theopening in the aperture plates. If aperture clogs aresuspected, first perform an Auto Clean procedure.See Section 3.3: Auto Clean. If the fault persists,clean the aperture plate using the procedures inthis section.Open Front Covers (Remove Lower Cover, ifapplicable). Refer to the CELL-DYN 1800System Operator’s Manual, Section 2:Installation Procedures and Special Requirements,Installation, Inspection and Tubing Installation,Opening/Removing Front Covers.5Press [PROBE DOWN].3ockChe2Materials needed: Protective equipment (gloves, lab coatand eye protection). Deionized water for rinsing. Cleaning solution (20 drops of CELL-DYNEnzymatic Cleaner to 20 mL of warmdeionized water OR 5 mL of 5% sodiumhypochlorite (bleach) to 15 mL of warmdeionized water). Small beaker or cup (50 mL) Aperture brush Microscope (optional)StCELL-DYN 1800 System Quick Reference GuideFrom the MAIN menu, press [SPECIALPROTOCOLS], followed by [MORE] then[PROBE HOME].m5ck.co3.4.1 CELL-DYN 1800 AperturePlates Cleaning Procedure3-10Press [DRAIN BATHS]. Liquid in bothchambers of the von Behrens RBC/PLT andWBC transducers drains to the waste system.149140393A April 2004
01 Jun 200412:45mRightCELL-DYN 1800 System Quick Reference GuideCleaning solution containing 20 drops ofEnzymatic Cleaner to 20 mL of warmdeionized water OR 5 mL of 5% sodiumhypochlorite (bleach) to 15 mL of warmdeionized water.Locate the two tagged red levers attached tothe von Behrens RBC/PLT and WBCtransducers. The von Behrens WBCtransducer bath is located on the left side of thefront panel and the von Behrens RBC/PLTtransducer bath is located on the right side.ockCheNOTE:5On the right side of the instrument, grasp theRBC/PLT aperture plate, located in the slotseparating the two chambers of the vonBehrens RBC/PLT transducer. Pull the aperatureplate straight out. Note that the aperture plate isetched with the letters R/P.8StOn the left side of the instrument, grasp the WBCaperture plate, located in the slot separating thetwo chambers of the von Behrens WBCtransducer. Pull the aperture plate straight out.Note that the aperture plate is etched with theletters WBC.6Grasp each lever and swing it all the way toyour right.7ck.coPrepare a fresh cleaning solution into a smallbeaker or cup.9140393A April 2004.9Immerse the aperture plates in the cleaningsolution for about 5 minutes Remove theaperture plates from the solution. Using theaperture brush only, gently clean both sides of theaperture plate using a rotating motion to clean theplate. Dab the bristles into the center hole todislodge any large particles.10NOTE:Using other brushes or paper willdamage the aperture plates.3.4.1 CELL-DYN 1800 Aperture Plates Cleaning Procedure3-11
Left01 Jun 200412:453.4.1 CELL-DYN 1800 Aperture Plates Cleaning Procedure3-1211Identify the aperture plate etched with R/P.Position the RBC/PLT aperture plate with theorientation notch towards the lower edge.Insert the aperture plate into the slot between thetwo chambers of the von Behrens RBC/PLTtransducer, located to the right of the aspirationprobe. Slide the aperture plate until it is completelyseated in the slot. Swing the red lever all the way toyour left to secure the plate in place.Identify the aperture plate etched with WBC.Position the WBC aperture plate with theorientation notch towards the lower edge.Insert the aperture plate into the slot between thetwo chambers of the von Behrens WBCtransducer, located to the left of the aspirationprobe. Slide the aperture plate until it is completelyseated in the slot. Swing the red lever all the way toyour left to secure the plate in place.Press [REFILL BATHS]. Both transducersare refilled. Check the right-hand chambersof both transducers to ensure they arecompletely filled with liquid.14Press [PROBE HOME]. Reattach and/orclose the front covers.Press [PROBE DOWN]. Press [MAIN] toreturn to MAIN menu.ck.coockCheNOTE:12mRinse each plate thoroughly with a finestream of deionized water. Gently shakeexcess water from the plate beforereinstalling it. DO NO
CELL-DYN 1800 System Quick Reference Guide 9140393A April 2004 FOREWORD The CELL-DYN 1800 System is manufactured by Abbott Diagnostics, Abbott Laboratories, 2000 Abbott Park Road, Abbott Park, IL 60064, USA. Please direct
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Manual Methodologies/ Semi-automated 3 3 CBC-3K Whole Blood Control (Five-Part WBC Differential) CBC-3K is a tri-level control for monitoring the Abbott CELL-DYN SAPPHIRE hematology analyzers. The CELL-DYN SAPPHIRE assay tables include values for 26 parameters. Assay values are also provided for the CELL-DY
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