Strata Silica-Based Solid Phase Extraction (SPE) Sorbents

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Strata Silica-BasedSolid Phase Extraction (SPE) Sorbents25 Cleanup Solutions for a Variety of Samples

Fact or Fiction?All Silica-BasedSPE Sorbents arethe SameFiction. Strata SPE products embody quality andperformance. Our extensive quality control proceduresprovide trouble-free solid phase extraction methods.Cleaner ExtractsInert material leads to cleaner extractsIn this comparison test, Strata C18-E gives cleaner extracts than manufacturer’s alternative solutions.PhenomenexStrata C18-E*Waters Sep-Pak C18*Biotage IST ISOLUTE C-18(EC)*Varian Bond Elut C18*Contact Phenomenex for details regarding this method. Comparative separations may not be representative of all applications. Waters and Sep-Pak are registered trademarksof Waters Corporation. Biotage, IST and ISOLUTE are registered trademarks of Biotage. Varian is a registered trademark of Varian, Inc. Bond Elut is a registered trademark ofAgilent Technologies, Inc.Reproducible ResultsConsistent manufacturing and QC ensures reproducible resultsThis study shows recovery data for 5 different lots of Strata C18-E 200 mg/3 mL tubes. Strata provides high, consistent and reliablerecoveries for 3 different drug compounds every time.6040201Lot 100Absolute Recovery %Absolute Recovery %02800120NADOLOL1001Lot Number2345PROPRANOLOL100Absolute Recovery %1208060402001Lot Number2345

ContentsLearn More About Strata SPE SorbentsWith 25 sorbents to choose from, we’ve highlighted thebenefits of each to make your sorbent selection easier.04 - 05Select Your Perfect MatchSelect your Strata SPE sorbent by using our StrataSelection Tree or use the Cross-Reference Chart tochoose a guaranteed alternative to your currentSPE sorbent.06 - 07Develop Your Method in 3 Easy Steps08 - 09Start Your Method Now10Applications:Search Our Website11Industry Applications: We’ve Done the Work for YouEnvironmental: Cleanup of Polycyclic Aromatic Hydrocarbons (PAHs) Improved Diesel FractionationFood and Beverage: Simultaneous Extraction of Melamine and Cyanuric Acid Cleanup of Aflatoxins from Peanut ButterOrdering Information12 - 1314 - 1516 - 19www.phenomenex.com03

Learn MoreAbout Strata SPESorbents Strata silica-based SPE sorbents are available in reversedphase, normal phase, ion-exchange and specialtysorbents. Use the chart below to learn more about StrataSPE sorbents and the additional benefits each phase canbring to your extraction methods.Reversed Phase SorbentsTypical ApplicationAdditional BenefitsPhaseExtraction ofhydrophobic or polarorganic analytesfrom aqueousmatricesExtraction of hydrophobic moleculesC18-ESorbent ChemistryOOSiOOSiSiOOEnhanced cleanup of hydrophobiccompounds that contain hydroxy or aminefunctional groupsC18-UOOSiOOSiOHOWide pore for the extraction of largehydrophobic molecules (up to 75 kDa)C18-TOOSiOOSiSiOOExtraction of extremely hydrophobiccompounds that are retained too tightly onC18-EC8Extraction of aromatic compoundsPhenylOOSiOOSiSiOOOOSiOOSiSiOOExtraction of non-polar compounds that areretained too tightly on C18-ECNOOSiCNOOSiOHOSDB-LTypical ApplicationAdditional BenefitsPhaseExtraction of polaranalytes fromnon-polar organicsolventsExtraction of polar compoundsCNn))Extraction of non-polar and polarcompounds; pH resistant sorbentNormal Phase SorbentsSorbent ChemistryOOSiCNOOSiOHOExtraction of strong anionsNH2OONH2SiOOSiNH2OExtraction of polar compounds that aresimilar in structureSilicaOOSiOHOOOExtraction of pesticides04www.phenomenex.comFlorisil (FL-PR)FlorisilSiOH

Ion-Exchange SorbentsTypical ApplicationAdditional BenefitsPhaseExtraction ofcharged analytesfrom aqueous ornon-polar organicsamplesExtraction of quaternary aminesWCXSorbent ChemistryOOOSiOHOOSiOHOExtraction of 1 , 2 , and 3 aminesSCXOOOSiSOOSiO HOOHOMixed-mode cation-exchange that alsoprovides hydrophobic retentionScreen-COOSiNClNClOOSiOLarge particle size, mixed-mode cationexchange that also provides hydrophobicretentionScreen-C GFExtraction of strong anionsNH2OOSiOOSiOOONH2SiOOSiNH2OExtraction of weak anionsSAXOOCl-NSiOOSiOHOMixed-mode anion-exchange that alsoprovides hydrophobic retentionScreen-AOOClNSiOOSiOFractionation of neutral compounds such asamides from acidic and basic analytesOABWOOSSiOOO HOSiONH2Specialty SorbentsTypical Application and Additional BenefitsPhaseSorbent ChemistryExtraction of polar compounds from food and environmental samplesAlumina-N(AL-N)ProprietaryExtraction of hydrocarbons from environmental samples whilesimultaneously removing excess waterEco-ScreenProprietaryFractionation of aliphatic and aromatic hydrocarbons fromenvironmental samplesEPHOOSiOHOOSiOHOSimultaneous extraction of melamine and cyanuric acid from food andbiological samplesMelamineProprietaryExtraction of polycyclic aromatic hydrocarbons (PAHs) from watersamples as defined in EPA 550.1 while simultanously removing humicacidsPAHProprietaryRemoval of aqueous residues from organic solutions in an effort toreduce blow-down timeSodium SulfateSodium Sulfatewww.phenomenex.com05

Select YourPerfect MatchUse the Strata Selection Tree below to find the sorbentthat is best for your analysis.Strata Selection eutralneutral( rmoderatelypolarpolar( een-CPhenylPhenylEco-ScreenNH2Screen-C GFScreen-C GFAL-NIf the Strata SPE products in this brochure do not provide at least anequivalent separation as compared to other products of the same phaseand comparable dimensions, send in your comparative data within 45 daysand keep the product for FREE.06ionicwww.phenomenex.com

Cross-ReferenceChartPhenomenexStrataThe below Strata products are guaranteed alternatives tocompetitive products listed.Waters Sep-Pak Agilent SampliQ Varian Bond Elut Supelco Discovery tC18SampliQ C18ECBond Elut C18DSC-18UCT JT BAKER BakerbondBiotage IST ISOLUTE MachereyNagel Chromabond C18OctadecylC18 (EC)C18ecLight LoadOctadecylC18C18Reversed PhaseC18-EC18-UBond Elut C18-OHC18-TC18Bond Elut C18-EWPDSC-18LtC18ec fC8C8SampliQ C8 OctylBond Elut C8DSC-8C8OctylC8(EC)Phenyl (PH)SampliQ PhenylBond Elut PHDSC-PhPhenylPhenylPHSDB-LSampliQ DVBBond Elut ENVBond Elut LMSDSC-PS/DVBStyreScreen DVB H2O-phobic DVB101HR-P C6H5C8Normal PhaseSi-1 (Silica)SilicaSampliQ SilicaBond Elut SIDSC-SiSilicaSilica GelSISiOHFL-PR (Florisil )Florisil SampliQ Florisil PRBond Elut Florisil ENVI-Florisil Florisil PRFlorisil FLFlorisil NH2NH2SampliQ Amino (NH2)Bond Elut Aminopropyl(NH2)DSC-NH2Amino PropylAminoNH2NH2CNCNSampliQ Cyano (CN)Bond Elut Cyano (CN-E)DSC-CNCNCyanoCNCNSAXSampliQ Si-SAXBond Elut SAXDSC-SAXQuaternary Amine Quaternary AmineSAXSBSCXSampliQ Si-SCXBond Elut SCXDSC-SCXBenzene SulfonicAcidAromatic SulfonicAcidSCX-3SAWCXBond Elut CBADSC-WCXCarboxylic AcidCarboxylic AcidCBAPCAScreen-CSampliQ C8/Si-SCXMixed ModeBond Elut Certify Clean Screen DAUNarc -2HCXDrugScreen-C GFBond Elut Certify I HFXtract DAUScreen-ABond Elut Certify IIClean ScreenTHCNarc -1HAXIon-ExchangeABWwww.phenomenex.com07

Develop YourMethod in 3 EasyStepsFollow Steps 1 through 3 to select the correct sorbentmass, format, and wash and elution volumes foryour work.Step 1. Choose the correct sorbent massSample MatrixSample VolumeSuggested Sorbent MassBlood250 µL50 mgSerum250 µL50 mgPlasma250 µL50 mgUrine500 µL50 mgFiltered tissue homogenates100 mg100 mgWater (particulate-free), drinking100-500 mL500 mgWater (particulate-laden), rivers, runoff, etc.100-500 mL1gSoil extracts100 g1gStep 2. Choose the correct formatSample VolumeNumber of Samples96-Well Plates 1 mL 96On-LineCartridges 6 mL 96 6 mL08Giga Tubeswww.phenomenex.comTraditionalSPE Tubes

Step 3. Optimize your wash and elutionSorbent Wash and Elution VolumesThe volume of solvent needed for SPE processing is directly related to the mass of sorbent in the SPE tube, and more specifically, the “bedvolume” of the SPE device. Intuitively we know more sorbent requires more solvent, less sorbent less solvent. Typically, 4 – 16 bed volumesare used in SPE methods.Silica-Based Sorbent MassPractical Minimum Wash and Elution Volume4 bed volumesRecommended Wash and Elution Volume8 bed volumes10 mg60 µL120 µL50 mg300 µL600 µL100 mg600 µL1.2 mL150 mg900 µL1.8 mL200 mg1.2 mL2.4 mL500 mg3 mL6 mL1g6 mL12 mL2g12 mL24 mL5g30 mL60 mL10 g60 mL120 mLwww.phenomenex.com09

Start YourMethod NowPhenomenex has designed general starting methodsfor reversed phase, normal phase, and ion-exchangeextractions.Strata ReversedPhase MethodStrata Ion-ExchangeMethodStrata Normal PhaseMethodfor C18, C8, Phenyl, CN, SDB-L Sorbentsfor SCX, WCX, SAX, NH2 (WAX) Sorbentsfor Silica, Florisil , NH2, CN Sorbents 1ANALYTE PROPERTIESModerate to high polarity us; low ionic strength buffers ( 30 mM), pH adjusted2CONDITIONING STEP1. Conditioning – polar organic solvents2. Equilibration – low ionic strength buffers, pH adjustedSAMPLE/MATRIXNon-polar organic solvents or moderately polarorganic solvents3CONDITIONING STEP1. Solvation – polar organic solvent2. Equilibration – sample/matrix solvent (non-polar)4WASH STEPNon-polar organic solvents with a low concentration(1 to 5 %) of moderate to low polarity organic solvents5ELUTION STEPNon-polar organic solvents containing higherconcentrations (5 to 50 %) of moderate to highpolarity organic solvents1ANALYTE PROPERTIESLow to moderate polarity (or non-polar), hydrophobic,neutralized/unchargedANALYTE PROPERTIESIonized/charged compounds22SAMPLE/MATRIXAqueous, diluted with buffer33CONDITIONING STEP1. Solvation – polar organic solvents2. Equilibration – aqueous, buffers144WASH STEPAqueous buffers with 5 to 50 % polar organic solvent5ELUTION STEPPolar or non-polar organic solvent(s) with or withoutwater, buffer and/or strong acid or base5WASH STEPAqueous buffers of low salt concentrations withor without organic solventELUTION STEP Neutralize the charge on the weak anion or cation Increase the ionic strength and counter ion concentration Add a strong counter ion displacer Elute using a polar organicSuggested Elution SolventsPolaritySuggested Elution SolventsSuggested Elution Solvents THFIncreasingPolarityFor complete ionization sample should be adjusted 2pH units above or below the pKa of analyte. pH can beused to effectively neutralize sorbent or analyte. Thiscan be accomplished by combining 2 % strong acidor base with a water miscible organic solvent such asmethanol or acetonitrile. [ As an alternative method,high ionic strength buffer can be used to displace theanalyte, which may not be ideal for analysis by sensitivedetection instruments such as a mass spec]. Hexane Acetone Ethyl Acetate Acetonitrile** Isopropanol MethanolPolar** when using aromatic sorbents such as Phenyl orSDB-L, acetonitrile is a stronger elution solvent thanmethanol **Create a Customized SPE Method in Under 1 Minute!Visit: www.phenomenex.com/info/MDTOOLto use our Online SPE Method Development Tool10www.phenomenex.com Methylene Chloride THFPolarityMost NonpolarIncreasingPolarity Acetone Acetonitrile IsopropanolPolar

ApplicationsPhenomenex has compiled a database of SPE, HPLC,and GC applications and now you have access to it!Search ourwebsiteSearch by the following: Compound Class Technique Separation Mode Compound Category Method Pharmacological EffectView 1,000’s of applications online m11

IndustryApplicationsStrata PAHEnvironmentalProvides excellent cleanup and recoveriesof polycyclic aromatic hydrocarbons(PAHs) as defined in EPA 550.1 whilesimultaneously removing humic acids whichcause chromatographic interferences.Strata PAHStrata PAH 1.5 g / 6 mL SPE Tubes (Part Number 8B-S130-7CH)1Condition:- 20 mL Dichloromethane- 20 mL Methanol- 20 mL D.I. Water2Load:- 100 µL PAH standards (100 µg/mL in Acetonitrile) spiked into 100 mLWater/Acetonitrile (75:25)3Wash:- 5 mL Methanol/D.I. Water (50:50)4Dry:- 15 seconds under 10” Hg vacuum5Elute:- 6 mL DichloromethaneEffective Removal of Humic AcidsGC Analysis of Polycyclic Aromatic Hydrocarbons (PAHs)19651Abundance108000000Intensity, 216151443.5e819 20173.0e818App ID 1965130000004.5e83200000010000002.5e8App ID 1966275000000Humic Acids Extract from Strata lumn:Dimensions:Part No.:Injection:Carrier Gas:Oven Program:Zebron ZB-5ms30 meter x 0.25 mm x 0.25 μm7HG-G010-11Split 15:1 @ 310 C, 1 μLHelium @ 1.4 mL/min (constant flow)140 C to 240 C @ 15 C/min to 275 C @ 4 C/min to 320 C @ 10 C/min for 5 minDetector: MS @ 270 C11. PyreneSample: 1. D8-Naphthalene12. Benz[a]anthracene2. Naphthalene13. Chrysene3. 2-Methylnaphthalene14. Benzo[b]fluoranthene4. Acenaphthalene15. Benzo[k]fluoranthene5. Acenaphthene16. D12-Benzo[a]pyrene6. Fluorene17. Benzo[a]pyrene7. Anthracene18. Indeno[1,2,3-cd]pyrene8. Phenanthrene19. Dibenzo[a,h]anthracene9. Fluoranthene20. Benzo[g,h,i]perylene10. D10-Pyrene12www.phenomenex.com202224 minHumic Acids Extract from Strata-PAH3.004293.05436Column:Dimensions:Part No.:Mobile Phase:Gradient:Flow 494Kinetex 2.6 µm C850 x 2.1 mm00B-4497-ANA: 5 mM Ammonium acetateB: MethanolTime (min) B (%)0152956956.01150.4 mL/minAmbientMS @ 580.4 amu / 536.5 amu (ambient)210 barHumic Acids from Suwannee 05443.855513.905583.95565 minFor More ApplicationsVisit:www.phenomenex.com/application

IndustryApplicationsStrata EPHSpecialized SPE sorbent designed to helpovercome the challenges associated withtraditional silica gel fractionation of aliphaticand aromatic hydrocarbons.EnvironmentalStrata EPHStrata EPH 5 g / 20 mL SPE Tubes, Teflon (Part Number 8B-S031-LEG-T)1Condition:- 30 mL Hexane2Load:- sample diluted in hexane3Elute Aliphatics:- 11 mL Hexane4Elute Aromatics:- 20 mL Methylene chlorideContamination Level of BHT from Strata EPH and Conventional SPE TubesLower levels of BHT contamination incomparison to conventional silica gelSPE tubes.Strata EPHConventionalsilica-gelAliphatic FractionAromatic Fraction0 10 20 30 40 50 60 70 80BHT concentration (ppm)1578715788GC Analysis of Aliphatic and Aromatic Fractions6781221314101511175510156820259 1013 15161024930App ID 1578743191820122221523714min0Figure 1. Representative chromatogram of the aliphatic fraction.The same running conditions were used to separate the Aliphatic and Aromatic fractions.Column: Zebron ZB-5msDimensions: 30 meter x 0.32 mm x 0.25 µmPart No.: 7HM-G010-11Injection: Splitless @ 285 C, 2 µLCarrier Gas: Helium @ 3 mL/min (constant flow)Oven Program: 60 C for 1 min to 290 C at 8 C/min for 6.75 minDetector: Flame Ionization (FID) @ 315 CAliphatic Fraction: 1. C910. 5-α-Androstane (IS)Aromatic Fraction:2. C1011. 1-Chloro-Octadecane (surr)3. C1212. C224. C1413. C245. Butylhydroxytoluene14. C266. C1615. C287. C1816. C308. C1917. C369. C20171611App ID 15788135101520253035minFigure 2. Representative chromatogram of the aromatic thalene2-Fluorobiphenyl (frac surr)Acenaphthalene2-Bromonaphthalene (frac surr)AcenaphthenePhthalateFluorenePhenanthrene10. Anthracene11. 0-Terphenyl (surr)12. 5-α-Androstane13. Fluoranthene14. 1- Chloro-Octadecane (surr-aliphatic)15. Pyrene16. Benz[a]anthracene17. Chrysene18. Benzo[b]fluoranthenewww.phenomenex.com19. Benzo[k]fluoranthene20. Benzo[a]pyrene21. Indeno[1,2,3-cd]pyrene22. Dibenz[a,h]anthracene23. Benzo[g,h,i]perylene13

IndustryApplicationsStrata MelamineSimultaneously extract melamine andcyanuric acid from food samples, using oneSPE sorbent.Food and BeverageProtein Precipitation1.2.3.Add 1 mL of sample (spiked with Internal Standard, IS), 100 µL of 0.2 N HCl,and 3 mL of Acetonitrile to centrifuge tubeVortex and centrifuge at 6000 rpm for 10 minCollect the supernatant for Strata Melamine cleanupGreater than 90 % Recoveries% Recovery100 90 -Strata Melamine Cleanup60 50 40 30 -Melamine115 %70 -Sorbent: Strata Melamine, 200 mg/ 3 mL tubePart No.: 8B-S049-FBJCondition: 1) 3 mL Methanol @ 1 mL/min2) 3 mL Acetonitrile/Water (50:50) @ 1 mL/minLoad: Collected supernatant from centrifugation step in proteinprecipitation prior to SPEWash 1: 1 mL Acetonitrile/Water (50:50); 2x 500 µLWash 2: 500 µL Methanol/Water (50:50)Dry: 2 min at 10" of HgElute: 1) 500 µL Methanol2) 2x 500 µL 5 % Ammonium hydroxide in MethanolCyanuric Acid94.5 %80 -20 10 0-LC analysis on baby formula extraction1828721MS switched from -vemode to ve modeApp ID 18287Flow 2.02.22.4Cyanuric Acid2.62.83.03.23.4 minColumn:Dimensions:Part No.:Injection:Carrier Gas:Oven Program:Detector:Sample:3,4App ID 182651,2234Luna 3 µm HILIC100 x 2.0 mm00D-4449-B0A: AcetonitrileB: 100 mM Ammonium formate pH 3.2A/B (90:10)0.4 mL/minMass Spectrometer (MS)1. Cyanuric acid (-ve)2. Melamine ( ve)MelamineGCanalysis on baby formula extraction1826514Column:Dimensions:Part No.:Mobile Phase:56www.phenomenex.com789 minZebron ZB-XLB-HT Inferno15 meter x 0.25 mm x 0.25 µm7EG-G024-11On-Column @ 103 C, 1 µLHelium @ 1.4 mL/min (constant flow)100 C for 0.5 min to 320 C @ 25 C/minMass Selective (MSD) @ 325 CAnalytes are 200 ng / 100 µL in BSTFA / Pyridine (1:1)1. Cyanuric Acid 13C3 (IS)2. Cyanuric Acid3. Melamine 13C3 15N3 (IS)4. Melamine

IndustryApplicationsFood and BeverageStrata Si-1 (silica)and FL-PR(Florisil )A two-stage SPE procedure is effectivein removing detector interferingcontaminants from a peanut butter matrixwhile maintaining absolute recoveries ofaflatoxins above 80 %.Strata Florisil 500 mg/3 mLStrata Silica (Si-1) 200 mg/3 mLPart No.: 8B-S013-HBJPart No.: 8B-S012-FBJPretreatment: To 5 g of peanut butter, add 40mL Methanol/Water (80:20) containing 0.2 g ofsodium chloride. Mechanically stir for 2 hours.Filter residual solids with a Whatman filterpaper and rinse 3x with 5 mL Methanol. Dryextracts over anhydrous magnesium sulfate,dry solvent under Nitrogen at 45 C andreconstitute in 500 µL Methanol/Water (80:20).Condition: 2 x 3 mL of hexane Load: 2 mL of reconstituted sample from theStrata Florisil Wash: 1. 2 x 2 mL of methanol/chloroform (1:1) 2. 1 x 1 mL of methanol/chloroform (1:1)Condition: No conditioning was performed asthis led to reduced recoveries of aflatoxins Load and wash solutions from the silica SPEwere pooled together and dried down undernitrogen and reconstituted in 500 µL of themobile phase used for LC/UV or LC/MSanalysisLoad: A 1.5 mL aliquot of peanut butterextract was spiked with afl atoxin standardsand loaded Wash: 1. 2 x 3 mL of methanol/water (80:20)2. 2 x 3 mL of 100 % methanol Elute: 2 x 3 mL of acetone/water/0.5 %formic acid (96:3.5:0.5) The combined eluate was dried undernitrogen and the residue reconstituted in 2mL of 1:1 hexane/chloroform and loaded ontothe Strata Silica cartridge for further cleanupColumn:Dimensions:Part No.:Mobile Phase:LC/MS/MS Chromatogram of Aflatoxin Standards at 50 00App ID 1864260050040030020010000.51.01.52.02.53.0Flow Rate:Temperature:Detection:Sample:min4.0minKinetex 2.6 μm PFP50 x 2.1 mm00B-4477-ANA: 0.1 % Formic acid and 5 mM Ammonium acetatein WaterB: 0.1 % Formic acid and 5 mM Ammonium acetatein MethanolTime (min) B (%)0500.25502702.01952.5952.51504.450400 µL/min25 CMass Spectrometer (MS)1. Aflotoxins M1(IS)2. Aflotoxins G23. Aflotoxins G14. Aflotoxins B25. Aflotoxins B1www.phenomenex.com15

Ordering InformationFor Large Scale Cleanup Available in 12, 20, 60, and 150 mLvolume tube sizes Pre-packed formats eliminate excesslabor associated with glass packedcolumnsFor On-line Screening 1-3 minute run time Direct inject analysisFor Traditional SamplePreparation Compatible with most manifolds androbotic workstations Consistent well-to-well and tube-totube flowFor Flash Analysis Wide range of polar and non-polarselectivities Gram to kilogram quantities16www.phenomenex.com

Reversed PhaseStrata C18-ESorbentMassPart No.Tube50 mg8B-S001-DAK100 mg 8B-S001-EAK100 mg 8B-S001-EBJ200 mg 8B-S001-FBJ200 mg 8B-S001-FCH500 mg 8B-S001-HBJ500 mg 8B-S001-HCHGiga Tube500 mg 8B-S001-HDG2g8B-S001-KDG5g8B-S001-LEG10 g8B-S001-MFF20 g8B-S001-VFF50 g8B-S001-YSN70 g8B-S001-ZSN96-Well Plate25 mg8E-S001-CGB50 mg8E-S001-DGB100 mg 8E-S001-EGBUnit/BoxPrice1 mL (100/Box)1 mL (100/Box)3 mL (50/Box)3 mL (50/Box)6 mL (30/Box)3 mL (50/Box)6 mL (30/Box)CH0-5845Unit/BoxeaSorbentMassPart No.Tube100 mg 8B-S004-EAK200 mg 8B-S004-FBJ500 mg 8B-S004-HBJ500 mg 8B-S004-HCH1g8B-S004-JCH96-Well Plate25 mg8E-S004-CGB50 mg8E-S004-DGBPriceeaStrata C8SorbentMassPart No.Tube100 mg 8B-S005-EAK200 mg 8B-S005-FBJ500 mg 8B-S005-HBJ500 mg 8B-S005-HCH1g8B-S005-JCHGiga Tube2g8B-S005-KDG5g8B-S005-LEG10 g8B-S005-MFF96-Well Plate25 mg8E-S005-CGB50 mg8E-S005-DGB100 mg 8E-S005-EGBUnit/BoxPrice1 mL (100/Box)3 mL (50/Box)3 mL (50/Box)6 mL (30/Box)6 mL (30/Box)12 mL (20/Box)20 mL (20/Box)60 mL (16/Box)2 Plates/Box2 Plates/Box2 Plates/BoxPart No.00M-S101B0-CBCH0-5845Unit/BoxeaPrice1 mL (100/Box)3 mL (50/Box)3 mL (50/Box)6 mL (30/Box)2 Plates/Box2 Plates/BoxUnit/BoxPriceSorbentMassPart No.Tube100 mg 8B-S007-EAK200 mg 8B-S007-FBJ500 mg 8B-S007-HBJ500 mg 8B-S007-HCH1g8B-S007-JCHGiga Tube2g8B-S007-KDG96-Well Plate50 mg8E-S007-DGB100 mg 8E-S007-EGBUnit/BoxPrice1 mL (100/Box)3 mL (50/Box)3 mL (50/Box)6 mL (30/Box)6 mL (30/Box)12 mL (20/Box)2 Plates/Box2 Plates/Box1 mL (100/Box)3 mL (50/Box)3 mL (50/Box)6 mL (30/Box)6 mL (30/Box)2 Plates/Box2 Plates/BoxStrata PhenylSorbentMassPart No.Tube100 mg 8B-S006-EAK200 mg 8B-S006-FBJ500 mg 8B-S006-HBJ500 mg 8B-S006-HCH1g8B-S006-JCH96-Well Plate25 mg8E-S006-CGB50 mg8E-S006-DGB100 mg 8E-S006-EGBUnit/BoxPrice1 mL (100/Box)3 mL (50/Box)3 mL (50/Box)6 mL (30/Box)6 mL (30/Box)2 Plates/Box2 Plates/Box2 Plates/BoxStrata SDB-LOn-Line Extraction CartridgeDescriptionStrata C8 on-lineextraction cartridge,20 x 2.0 mmCartridge holder,20 mm(can also be used for Normal Phase)Unit/Box(wide pore)2 Plates/Box2 Plates/Box2 Plates/BoxPart No.00M-S039B0-CBSorbentMassPart No.Tube100 mg 8B-S002-EAK200 mg 8B-S002-FBJ500 mg 8B-S002-HBJ1g8B-S002-JCH96-Well Plate50 mg8E-S002-DGB100 mg 8E-S002-EGBStrata C18-T12 mL (20/Box)12 mL (20/Box)20 mL (20/Box)60 mL (16/Box)60 mL (16/Box)150 mL (8/Box)150 mL (8/Box)On-Line Extraction CartridgeDescriptionStrata C18 on-lineextraction cartridge,20 x 2.0 mmCartridge holder,20 mmStrata CNStrata C18-U PriceSorbentMassPart No.Tube100 mg 8B-S014-EAK200 mg 8B-S014-FBJ200 mg 8B-S014-FCH500 mg 8B-S014-HBJ500 mg 8B-S014-HCH1g8B-S014-JCHGiga Tube2g8B-S014-MFF96-Well Plate50 mg8E-S014-DGBUnit/BoxPrice1 mL (100/Box)3 mL (50/Box)6 mL (30/Box)3 mL (50/Box)6 mL (30/Box)6 mL (30/Box)60 mL (16/Box)2 Plates/BoxeaAdditional sizes and sorbentmasses available. For a completelist of Strata SPE products visit:www.phenomenex.comwww.phenomenex.com17

Ordering InformationNormal PhaseCation-ExchangeStrata NH2 / WAXStrata WCX (can also be used for anion-exchange)SorbentMassPart No.Tube100 mg 8B-S009-EAK200 mg 8B-S009-FBJ500 mg 8B-S009-HBJ500 mg 8B-S009-HCH1g8B-S009-JCHGiga Tube500 mg 8B-S009-HDG2g8B-S009-KDG5g8B-S009-LEG10 g8B-S009-MFF20 g8B-S009-VFF96-Well Plate25 mg8E-S009-CGB50 mg8E-S009-DGB100 mg 8E-S009-EGBUnit/Box(weak cation-exchange)Price1 mL (100/Box)3 mL (50/Box)3 mL (50/Box)6 mL (30/Box)6 mL (30/Box)12 mL (20/Box)12 mL (20/Box)20 mL (20/Box)60 mL (16/Box)60 mL (16/Box)2 Plates/Box2 Plates/Box2 Plates/BoxUnit/BoxPrice3 mL (50/Box)6 mL (30/Box)6 mL (30/Box)20 mL (20/Box)12 mL (20/Box)20 mL (20/Box)60 mL (16/Box)Strata Silica(Si-1)Unit/BoxPrice1 mL (100/Box)3 mL (50/Box)3 mL (50/Box)6 mL (30/Box)6 mL (30/Box)12 mL (20/Box)12 mL (20/Box)12 mL (20/Box)20 mL (20/Box)60 mL (16/Box)60 mL (16/Box)150 mL (8/Box)150 mL (8/Box)SorbentMassPart No.Tube100 mg 8B-S010-EAK100 mg 8B-S010-EBJ200 mg 8B-S010-FBJ500 mg 8B-S010-HBJ500 mg 8B-S010-HCH1g8B-S010-JCHGiga Tube2g8B-S010-KDG5g8B-S010-LEG10 g8B-S010-MFF20 g8B-S010-VFF96-Well Plate25 mg8E-S010-CGB50 mg8E-S010-DGB100 mg 8E-S010-EGB1 mL (100/Box)3 mL (50/Box)3 mL (50/Box)6 mL (30/Box)6 mL (30/Box)12 mL (20/Box)20 mL (20/Box)6 mL (16/Box)2 Plates/Box2 Plates/Box2 Plates/BoxUnit/BoxPrice1 mL (100/Box)3 mL (50/Box)3 mL (50/Box)3 mL (50/Box)6 mL (30/Box)6 mL (30/Box)12 mL (20/Box)20 mL (20/Box)60 mL (16/Box)60 mL (16/Box)2 Plates/Box2 Plates/Box2 Plates/BoxStrata Screen-C(mixed-mode cation-exchange)SorbentMassTube100 mg100 mg150 mg150 mg200 mg300 mg500 mgPart 6-SCH8B-S016-FBJ8B-S016-RBJ8B-S016-HCH1 mL (100/Box)3 mL (50/Box)6 mL (30/Box)6 mL (30/Box)3 mL (50/Box)3 mL (50/Box)6 mL (30/Box)Price2 Plates/Box2 Plates/BoxStrata CN(See under Reversed Phase for ordering information)18Price(strong cation-exchange)(pesticide residue grade)SorbentMassPart No.Tube100 mg 8B-S012-EAK200 mg 8B-S012-FBJ500 mg 8B-S012-HBJ500 mg 8B-S012-HCH1g8B-S012-JCHGiga Tube500 mg 10 g8B-S012-MFF20 g8B-S012-VFF50 g8B-S012-YSN70 g8B-S012-ZSN96-Well Plate50 mg8E-S012-DGB100 mg 8E-S012-EGBUnit/BoxStrata SCXStrata Florisil SorbentMassPart No.Tube500 mg 8B-S013-HBJ500 mg 8B-S013-HCH1g8B-S013-JCHGiga Tube1g8B-S013-JEG2g8B-S013-KDG5g8B-S013-LEG10 g8B-S013-MFFSorbentMassPart No.Tube100 mg 8B-S027-EAK200 mg 8B-S027-FBJ500 mg 8B-S027-HBJ500 mg 8B-S027-HCH1g8B-S027-JCHGiga Tube2g8B-S027-KDG5g8B-S027-LEG10 g8B-S027-MFF96-Well Plate25 mg8E-S027-CGB50 mg8E-S027-DGB100 mg 8E-S027-EGBwww.phenomenex.comAdditional sizes and sorbentmasses available. For a completelist of Strata SPE products visit:www.phenomenex.com

Anion-ExchangeSpeciality PhasesStrata SAX Strata EPH(strong anion-exchange)SorbentMassPart No.Tube100 mg 8B-S008-EAK100 mg 8B-S008-EBJ200 mg 8B-S008-FBJ500 mg 8B-S008-HBJ500 mg 8B-S008-HCH1g8B-S008-JCHGiga Tube500 mg 8B-S008-HDG2g8B-S008-KDG5g8B-S008-LEG10 g8B-S008-MFF20 g8B-S008-VFF96-Well Plate25 mg8E-S008-CGB50 mg8E-S008-DGB100 mg 8E-S008-EGBUnit/BoxPrice1 mL (100/Box)3 mL (50/Box)3 mL (50/Box)3 mL (50/Box)6 mL (30/Box)6 mL (30/Box)SorbentMassPart No.Tube500 mg 8B-S313-HBJ1g8B-S313-JCHGiga Tube2g8B-S313-KDG12 mL (20/Box)12 mL (20/Box)20 mL (20/Box)60 mL (16/Box)60 mL (16/Box)2 Plates/Box2 Plates/Box2 Plates/BoxSorbentMassPart No.Tube1g8B-S046-JBJPrice1 mL (100/Box)3 mL (50/Box)6 mL (30/Box)6 mL (30/Box)3 mL (50/Box)20 mL (20/Box)20 mL (20/Box)Unit/BoxPrice3 mL (50/Box)6 mL (30/Box)12 mL (20/Box)2 Plates/BoxUnit/BoxPrice3 mL (50/Box)Strata PAH(Polycyclic Aromatic Hydrocarbons)SorbentMassPart No.Tube750 mg 8B-S130-WCH1.5 g8B-S130-7CHUnit/BoxPrice6 mL (30/Box)6 mL (30/Box)MelamineStrata ABW(specialty phase)SorbentMassPart No.Tube1g8B-S030-JCHGiga Tube2g8B-S030-KDG5g8B-S030-LEG10 g8B-S030-MFF20 g8B-S030-VFFPriceStrata Eco-Screen(mixed-mode anion-exchange)Unit/BoxUnit/BoxStrata Alumina-N (AL-N)Strata Screen-ASorbentMassPart No.Tube100 mg 8B-S019-EAK200 mg 8B-S019-FBJ200 mg 8B-S019-FCH500 mg 8B-S019-HCH96-Well Plate25 mg8E-S019-CGBSorbentMassPart No.Tube500 mg 8B-S031-HBJGiga Tube5g8B-S031-LEGTeflon Giga Tube5g8B-S031-LEG-TUnit/BoxPrice6 mL (30/Box)12 mL (20/Box)20 mL (20/Box)60 mL (16/Box)60 mL (16/Box)Strata NH2 / WAX(See under Normal Phase for ordering information)SorbentMassPart No.Tube100 mg 8B-S049-EBJ200 mg 8B-S049-FBJ96-Well Plate50 mg8E-S049-DGBUnit/BoxPrice3 mL (50/Box)6 mL (30/Box)Trademarks2 Plates/boxSodium SulfateSorbentMassPart No.Tube1g8B-S124-JCHGiga Tube1g8B-S124-JEG5g8B-S124-LEGUnit/BoxTerms and ConditionsSubject to Phenomenex Standard Termsand Conditions, which may be viewed atwww.phenomenex.com/TermsAndConditionsPrice6 mL (30/Box)20 mL (20/Box)20 mL (20/Box)Strata, Kinetex, and Luna are registered trademarks, and Giga,Sepra, and Zebron are trademarks of Phenomenex, Inc. Agilentand SampliQ are registered trademarks of Agilent Technologies.Waters and Sep-Pak are registered trademarks of WatersCorp. Varian is a registered trademark of Varian, Inc. BondElut and Bond Elut Certify are registered trademarks of AgilentTechnologies, Inc. Sigma-Aldrich and Discovery are registeredtrademarks of Sigma-Aldrich, Inc. UCT, StyreScreen, CleanScreen and Xtract are registered trademarks of United ChemicalTechnologies. JT BAKER and POLAR PLUS are registeredtrademarks of Avantor Performance Materials. Narc is atrademark of Avantor Performance Materials. Biotage, IST andIsolute are registered trademarks of Biotage. Macherey-Nageland CHROMABOND are registered trademarks of MachereyNagel GmbH & Co. Florisil is a registered trademark of U.S.Silica Co. Teflon is a registered trademark of E.I. du Pont deNemours and Co.DisclaimerComparative separations may not be representative of allapplications.If Phenomenex products in this brochure do not provide at least anequivalent separation as compared to other products of the samephase and comparable dimensions, send in your comparative datawithin 45 days and keep the product for FREE.Additional sizes and sorbentmasses available. For a completelist of Strata SPE products visit:www.phenomenex.comPhenomenex, Inc. is not affiliated with Waters Corp., Varian,Inc., Sigma-Aldrich, Inc., United Chemical Technologies,Avantor Performance Materials, Biotage, Macherey-NagelGmbH & Co., or U.S. Silica Co. 2012 Phenomenex, Inc. All rights reserved.www.phenomenex.com19

Australiat: 02-9428-6444 f: 02-9428-6445 auinfo@phenomenex.comAustriat: 01-319-1301 f: 01-319-1300 anfrage@phenomenex.comBelgiumt: 31 (0)30-2418700 f: 31 (0)30-2383749 beinfo@phenomenex.comCanadat: (800) 543-3681f: (310) 328-7768 info@phenomenex.comDenmarkt: 4824 8048f: 4810 6265 nordicinfo@phenomenex.comFinlandt: 358 (0)9 4789 0063f: 45 4810 6265 nordicinfo@phenomenex.comFrancet: 01 30 09 21 10 f: 01 30 09 21 11 franceinfo

Sep-Pak Agilent SampliQ Varian Bond Elut Supelco Discovery UCT JT BAKER Bakerbond Biotage IST ISOLUTE Macherey-Nagel Chromabond Reversed Phase C18-E tC18 SampliQ C18EC Bond Elut C18 DSC-18 C18 Octadecyl C18 (EC) C18ec C18-U Bond Elut C18-OH Light Load Octadecyl C18 C18 C18-T C18 Bond Elut C18-EWP DSC-18Lt C18ec f C8 .

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