Laboratory Safety Manual - Maryland

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State Of MarylandMaryland Department of HealthLaboratories Administration1770 Ashland AvenueBaltimore, Maryland 21205Laboratory SafetyManual6.1 EditionISO QA-SOP-TR 5.03Manual Issued ToSeptember 2014

PREFACEThis Laboratory Safety Manual brings together information that will assist both you andyour supervisors to meet bio-security responsibilities through a formal safety program. “Biosecurity” refers to a broad program of preventive medicine designed to protect the health ofemployees who may encounter biological, chemical, or radiological hazards in the laboratory orfield.The success of our safety program depends upon you having the necessary knowledge tocarry out the program. When you are aware of risks, you are less likely to be a victim. Everyonewho works in a biological or chemical laboratory should have this knowledge. It includesknowing how to protect yourself and your co-workers, and how to respond to such laboratoryemergencies as exposure of an individual to, or contamination of the physical environment withdangerous microorganisms, radionuclides or chemicals.The degree to which safe practices are observed in a laboratory stems directly from theattitudes and actions of the people in charge. The responsibility for enforcing the SafetyProgram ultimately rests with the Laboratory Director; however, immediate responsibility restswith every supervisor and employee.Supervisors must see that each member of their staff understands the applicable contentsof this safety manual. Supervisors must also oversee and demand the observance of the policiesin the manual. Supervisors must educate themselves as fully as possible about potential hazards,and relay this information to their employees.Every employee is highly trained and a valuable resource. I do not want to see youinjured by a needless accident. Remember that you are the only person who can truly practicesafe procedures for your own protection and that of your fellow co-workers. Therefore, you arerequired to know, understand and adhere to the safety policies in this manual. They are designedto maintain your health.Robert Myers, Ph.D.DirectorLaboratories AdministrationThis Laboratory Safety Manual has been reviewed and approved for distribution to MDHLaboratories Administration employees.4/1/2018DateSignatureMDH – Laboratories AdministrationLaboratory Safety Manual: 6.1 Ed. 4/2018ii

State of MarylandMaryland Department of HealthLaboratories AdministrationAnnual Review of the Laboratory Safety ManualThe Laboratories Administration Laboratory Safety Manual will be reviewed on an annualbasis. This review may take the form of a Drill or Exercise, a Table-Top Exercise, an AfterAction Report/Review of an actual event, or a Document Review of the Safety Manual. Theannual review is a requirement to verify the Plan’s effectiveness, and will be documented usingthis form.This review is a (n) (check all that apply): Drill/ExerciseList all parties participating and the scenario used for the exercise. Include any pertinentproblems or questions that arise. Attach additional documentation to this sheet. Table-Top ExerciseList all parties participating and the scenario used for the exercise. Include any pertinentproblems or questions that arise. Attach additional documentation to this sheet. Document Review of the Laboratory Safety ManualA Document Review states that the Laboratory Safety Manual has undergone a thoroughevaluation, and does not require any additional modifications or further review at this time.No additional attachments are needed. After-Action Report/Review of an Actual EventIf an event requiring the implementation of the Laboratory Safety Manual takes place, theevent may be substituted for a Document Review and/or a Table Top Exercise. Attachadditional documentation providing a summary of the event, and a full explanation as to howthe Laboratory Safety Manual was employed, as well as any problems that arose pertainingto the Plan.Review Conducted By: Date:Safety & Security OfficerReview Signed Off By: Date:Laboratory Administration DirectorMDH – Laboratories AdministrationLaboratory Safety Manual: 6.1 Ed. 4/2018iii

Laboratory Safety ManualRevision HistoryREVISIONOriginalSecond VersionCOMMENTSUnited States Department of Labor, OccupationSafety and Health Administration (OSHA) mandatedthat all laboratories develop and enforce safetystandards for response to hazardous chemicalemergencies, employee awareness and right-to-knowchemical information, as well as laboratory safetystandards.DATEMarch 1988Laboratory Safety Manual revised.July 19882nd EditionLaboratory Safety Manual rewritten and updated.May 19943rd EditionLaboratory Safety Manual rewritten, reformatted, andupdated.January 20024th EditionLaboratory Safety Manual rewritten, reformatted, andupdated.October 20094.1 EditionLaboratory Safety Manual revise, contact informationupdated and ISO, QA-SOP-TR 5.03July 2010July 2011July 2012July 2013July 20145.0 EditionLaboratory Safety Manual rewritten and updated fornew facility 1770 Ashland Ave.5.1 Edition6.0 Edition6.1 EditionLaboratory Safety Manual reviewed and updated theglove personal protection equipment selectionguidelines and Appendix B.Laboratory Safety Manual revised and updated StateMaryland’s new health care provider WorkPro andOccupational Medical Services (OMS) effectiveApril 1, 2017.Laboratory Safety Manual revised and updated forthe name change of Department of Health & MentalHygiene to Maryland Department of Health, alongwith the addition of Appendix E Biosafety RiskAssessment Standard Operating Procedure.MDH – Laboratories ory Safety Manual: 6.1 Ed. 4/2018iv

TABLE OF CONTENTS1770 Laboratory Emergency Telephone Numbers .ix1.0OFFICE OF LABORATORY SAFETY AND SECURITY . 11.1Introduction.11.21.31.42.0EMERGENCY RESPONSE. 22.12.22.32.42.52.62.72.82.92.102.113.0Safety and Security Committee .1Chemical Hygiene Committee (CHC) .2Standing Subcommittees .2Accidents, Injuries, and Illnesses .2Chemical Spills .4Flammable Solvent Spills .5Nonflammable Spills .5Radioactive Injuries and Spills .6Biohazard Spills .8Managing Exposure Following Accidents Involving Infectious Agents .11Fire Prevention and Control .12Electrical Burns, Shocks, and Accidents .15Leaking Compressed Gas Cylinders .15Safety Showers and Eye Wash Stations.16GENERAL SAFETY POLICIES .173.1Visitor Access .173.2Smoking, Eating, and Drinking Policy .173.3Medical conditions notification .173.4Personal Safety Precautions .173.5Personnel Protective Equipment (PPE) .183.6Hand Washing.213.7Phlebotomy .223.8Glassware .223.9Centrifuging .223.10 Good Housekeeping .253.11 Identification Badge Policy (Refer to Security Plan & Manual) .253.12 Americans with Disabilities Act (ADA) .263.13 Biohazard Bags .263.14 Electrical Safety .263.15 Compressed Gas Safety .28MDH – Laboratories AdministrationLaboratory Safety Manual: 6.1 Ed. 4/2018v

3.164.0RIGHT-TO-KNOW LAW (HAZARDOUS COMMUNICATION) . 314.14.24.34.44.54.64.74.84.95.0General Radiation Safety Procedures .45Radiation Surveys .47Radiation Dose: Units and Protection Limits .48BIOLOGICAL SAFETY .497.17.27.37.47.57.67.78.0General Information and Required Procedures.37Routes of Exposure and Response .38Ordering and Procuring Chemicals .40Labeling Procedures and Warning Signs .40Storage of Chemicals .40RADIOLOGICAL SAFETY .456.16.26.37.0Categories of Hazardous Biological Agents .32Categories of Hazardous Chemicals .32Education and Training Programs .33Materials Safety Data Sheets (MSDS)/ Safety Data Sheets (SDS) .34Control Measure for Biological Agents .35Removal or Defacing Label .36Providing Information.37Employer's Education and Training Responsibility.37Employee Rights .37CHEMICAL LABORATORY SAFETY .375.15.25.35.45.56.0Elevator Use 1770 Central Lab .31Routes of Infection.49Vaccination/Immunization Requirements .50General Safety Policies and Practices for Microbiological Laboratories .51Laboratory Biosafety Level Criteria (from: BMBL, 5th Edition, December2009 revision) .53Universal Precautions for Laboratories .67Disinfecting .69Autoclave Safety .70WASTE DISPOSAL .708.18.28.38.48.5Infectious Waste Disposal.70Chemical Waste Disposal .72Waste Management Program .73Procedures for Disposing of Chemical Wastes and Unwanted Chemicals.73Glassware .74MDH – Laboratories AdministrationLaboratory Safety Manual: 6.1 Ed. 4/2018vi

9.0SPECIMEN RECEIPT, ACCESSIONING, and PREPARATIONAREAS .7410.0 GENERAL OFFICE SAFETY.7510.110.2Clerical and Administrative Area Hazards .75Security Hazards .7611.0 MAINTENANCE SHOP AND WAREHOUSE SAFETY .7612.0 REPRODUCTIVE SAFETY .7912.112.212.312.4Introduction .79Periconceptional Hazards.79Pregnant Employee .79Policy Regarding Pregnant Employees .80References .82Appendix A Employee First Report of Injury Forms .83Appendix B Chemical Classifications, Classes, Flash points, Boilingpoints, Glove Compatibility Resources, and Specific ChemicalIncompatibilities .92Appendix C Emergency Eyewash Station Weekly Check andInstructions .98Appendix D Radiation Dose, Units, Decay Tables .100Appendix E Biosafety Risk Assessment Standard Operating Procedure . 103MDH – Laboratories AdministrationLaboratory Safety Manual: 6.1 Ed. 4/2018vii

Key ContactsDirectorRobert A. Myers, Ph.D.Room 128A1770 Ashland AvenueBaltimore, Maryland 21205443-681-3800Safety and Security OfficerRachel V. MichaelOffice of Laboratory Safety & SecurityRoom 132C1770 Ashland AvenueBaltimore, Maryland 21205443-681-3792Radiation Safety OfficerMonica SaundersRoom 2301770 Ashland AvenueBaltimore, Maryland 21205443-681-3856Responsible OfficialJim SvrjcekOffice of Laboratory Emergency Preparedness and Response, Suite 1341770 Ashland AvenueBaltimore, Maryland 21205443-681-3787410-925-3121 (cell)Laboratory Personnel LiaisonDonyet BarnesRoom 128H1770 Ashland AvenueBaltimore, Maryland 21205443-681-3939Facility ManagerJack QuinlanRoom 132F1770 Ashland AvenueBaltimore, Maryland 21205443-681-3818MDH – Laboratories AdministrationLaboratory Safety Manual: 6.1 Ed. 4/2018viii

1770 Laboratory Emergency Telephone NumbersFire and AmbulancePolice/SecurityMedical EmergenciesLab AccidentsMaintenance ShopLab Waste DisposalSafety & Security OfficerRadiation SafetyPoison 856410-706-7701**For life threating emergencies dial 911.MDH – Laboratories AdministrationLaboratory Safety Manual: 6.1 Ed. 4/2018ix

1.0 OFFICE OF LABORATORY SAFETY AND SECURITY1.1IntroductionIn May 1986, the Director of the Laboratories Administration established the fulltime position of Safety & Security Officer (SSO) and the Office of Laboratory Safetyunder the Central Laboratory's Division of Laboratory Licensure, Certification andTraining. The Director also approved the establishment of a Laboratory Safety andSecurity Committee consisting of employees within organization. The Office ofLaboratory Safety is currently located within the Division of Administrative SupportServices. The SSO position and the Safety Committee were created to allow theorganization to:1.21.1.1Devote greater attention to health, safety and security issues affectingemployees.1.1.2Implement and maintain a full safety program for employees.1.1.3Ensure the Laboratories Administration meets all Maryland and FederalGovernment safety rules, regulations, and standards.Safety and Security Committee1.2.1Membership1.2.1.1 The Deputy Director of Administrative & Support Services, Safety andTraining, Quality Assurance Officer, and Radiation Safety Officer willserve as permanent members of the Committee.1.2.1.2 The SSO will serve as Chairperson and permanent member of both theCommittee and all standing subcommittees.1.2.1.3 One or more employees from each floor of the Central Lab will beappointed to the Committee by the SSO and Laboratory Director.Every Division should be represented.1.2.1.4 Members will be recruited by the SSO and/or Director, and appointedwith Director’s approval.1.2.1.5 All members are expected to serve a minimum of 12 months.1.2.1.6 Full committee meetings will be held bi-monthly, on the firstWednesday of even numbered months.1.2.1.7 Minutes of each full committee meeting will be taken, typed, anddistributed to each member. Each member is responsible for updatingMDH – Laboratories AdministrationLaboratory Safety Manual: 6.1 Ed. 4/20181

their Division Chief. Safety & Security will POST meeting minutes inthe Library and on the Laboratory Administration website for all LabEmployees.1.2.1.8 The SSO, in conjunction with the Director, may establish additionalsafety subcommittees as necessary.1.31.4Chemical Hygiene Committee (CHC)1.3.1Members will be recruited by the SSO, and appointed with the LaboratoryDirector’s approval.1.3.2Membership shall consist of five (5) employees that include a RadiationSafety Officer, two (2) senior level Division of Environmental Chemistryemployees, and at least two (2) active Safety & Security Committee members.1.3.3All members are expected to serve a minimum of 12 months.1.3.4Full committee meetings will be held quarterly or as needed.1.3.5The CHC is responsible for reviewing, revising, and updating the ChemicalHygiene Plan annually.1.3.6The SSO shall be responsible for providing reports to the Safety & SecurityCommittee.Standing Subcommittees1.4.1Safety Manual Revision/Review Subcommittee – To review, revise, or updatethe Safety Manual annually.2.0 EMERGENCY RESPONSE2.1Accidents, Injuries, and IllnessesA laboratory employee who experiences an accident, injury, or medical emergencyduring work hours, should take the following actions:MDH – Laboratories AdministrationLaboratory Safety Manual: 6.1 Ed. 4/20182

2.1.1Immediately assess the situation to determine the severity and danger to otheremployees.2.1.2Immediately inform your supervisor and the SSO of the situation. If anaccident occurs after hours, follow your Divisional call-in procedure, andinform the SSO as soon as possible.2.1.3If the injury or illness is severe (life threatening) or deemed to requireimmediate emergency medical care:2.1.3.1 Call 911 and the security emergency number 443-681-3911. Give acomplete and accurate description concerning the location and thenature of the incident.2.1.3.2 An employee must stay with the injured or ill person until help arrives.2.1.4For non-emergency injuries requiring medical attention, follow theseprocedures:2.1.4.1 Apply first aid according to the nature of the injury or illness, and takeappropriate action to minimize trauma to the injured person.2.1.4.2 Make arrangements to transport the injured person to the State’sdesignated health care facility [WorkPro and Occupational MedicalServices (OMS) locations]. See Appendix A- First Report of Injury.The supervisor or SSO should drive the patient to the clinic. Ifavailable, use the Administration State vehicle parked at the AshlandStreet Garage, 900 N. Washington Street.2.1.4.3 Get an AUTHORIZATION FOR EXAMINATION ORTREATMENT form from a Division Office, the SSO, (443-6813792), the Lab Personnel Officer (443-681-3939), or the MDH HumanResource Lab Liaison (410-767-5530). The form must be filled incompletely and have the appropriate signatures. See Appendix A.2.1.4.4 The injured person may then be taken to WorkPro and OccupationalMedical Services (OMS) location. See Appendix A for location andparking information.2.1.4.5 The employee accompanying the injured individual will not becharged leave. They should, however, clear their absence with theappropriate supervisor.2.1.5For minor cuts, abrasions, burns etc., apply first aid.MDH – Laboratories AdministrationLaboratory Safety Manual: 6.1 Ed. 4/20183

2.22.1.6In all cases, injuries and accidents must be reported using the proper forms asrequired by the Laboratories Administration. See Examples in Appendix A.Workman’s compensation will not pay for visits to the WorkPro orOccupational Medical Services (OMS) if a “First Report of Injury” form hasnot been filed. The date of injury must match on all forms. (See the SSO,Laboratory Personnel Liaison, Supervisor, or Division Chief for the necessaryforms.)2.1.7If the injury involves exposure to potentially infectious blood or other bodyfluids, professional counseling through the designated health care facilityshould be sought concerning the possibility of contracting a laboratoryacquired infection.2.1.8Provide the supervisor with a brief summary explaining the circumstances ofthe accident and/or injury, and in filling out all necessary forms. Submit anyassociated doctor’s slips to your supervisor, and bills to the MDH HumanResource Lab Liaison (410-767-5530).Chemical SpillsThe following procedures should be followed when chemical spills occur. Each Labspace containing a chemical is required to have an updated Lab Room ChemicalInventory and Materials Safety Data Sheet (MSDS)/Safety Data Sheets (SDS) bindercorresponding to each chemical inventoried.2.2.1Know the locations of each eyewash station(s) and safety shower(s) in yourwork area. Render First Aid: Attend to any person(s) who may becontaminated.2.2.1.1 If a liquid spill occurs over a large part of the body, removecontaminated clothes and quickly shower. Wash off all splatters ofchemicals using soap and water. Do not use neutralizing chemicals,topical ointments, or salves on the affected area.2.2.1.2 If a liquid spill involves the eyes, wash using an eyewash stationfor a minimum of 15 minutes. Pull eyelids away from the eye androll the eyeball up and down, and from side to side to thoroughly washthe eye; seek medical attention.2.2.1.3 If a dry chemical spill occurs, brush as much of the dry chemical offyour clothes and body as possible. Wash your face and handsthoroughly with soap and water. Eyes should be washed as describedin Section 2.2.2.2. NOTE: Sodium hydroxide (NaOH) and potassiumhydroxide (KOH) are difficult to wash off skin and clothing, as causticmaterials react with oils in the skin, and can penetrate through severalMDH – Laboratories AdministrationLaboratory Safety Manual: 6.1 Ed. 4/20184

layers. Washing should continue until the soapy (slick) feeling isgone. Seek medical attention if necessary.2.2.1.4 Some chemicals are corrosive, have volatile vapors, and are very toxic.If a spill occurs, to avoid exposure, evacuate the room, close thedoors, and wait for the laboratory air circulation system to clear thevapors and dry the spilled solvent. Do not re-enter the laboratory untilthe Lab Supervisor and Safety & Security Officer have been notified.Any employee entering the laboratory following a spill or lab accidentmust wear proper personal protective equipment.2.2.1.5 Give Warnings2.2.1.6 Evacuate nonessential personnel from the spill area.2.2.1.7 If the spill material is flammable, turn off all ignition and heat sources.2.2.1.8 Inform your immediate supervisor and the SSO.2.2.1.9 Initiate the appropriate cleanup procedure.2.32.4Flammable Solvent Spills2.3.1Turn off all gas burners but do NOT turn on or off any electrical equipment.Sparks from electric switches could start a fire.2.3.2For minor spills, cover with appropriate spill control material if available, orother appropriate absorbent material, to keep the spill from spreading. Waituntil the liquid is absorbed, and place material in a working fume hood toallow the solvent to evaporate.2.3.3Large spills may require the assistance of trained personnel and/or equipmentto perform an effective and safe cleanup. (Refer to Section 2.2.2.4.)Nonflammable SpillsIn each given work area, cleanup supplies and equipment on hand should be suitableto deal with a spill, and should be consistent with the potential hazards and quantitiesof chemicals in the area. Supplies should include neutralizing agents (refer toMSDS/SDSs; e.g., sodium carbonate, sodium bisulfite) and absorbents (e.g.,vermiculite, paper towels). Commercial spill cleanup kits containing similarmaterials and instructions covering their use are recommended, and should be locatedstrategically around technical work areas.2.4.1Acid SpillsMDH – Laboratories AdministrationLaboratory Safety Manual: 6.1 Ed. 4/20185

2.4.1.1 Wear rubber gloves and eye protection while pouring the contents ofan acid neutralizing spill kit (i.e. baking soda (NaHCO3), or sodiumsulfate (Na2SO4)) around the outer edge of the spill to neutralize theacid. Continue to add dry neutralizing material to the remainder of thespill area until the liquid has been absorbed.2.4.1.2 Wait 10-15 minutes, or until chemical neutralization is complete.Wear protective gloves and use a dustpan to pick up as much “mud” orwaste as practical. Put the “mud” in a plastic container for properdisposal.2.4.1.3 While wearing gloves, use paper towels to clean up the remaining mudand liquid. A wet mop or wet towels may then be used to wash thesurfaces. All towels should be placed in a plastic container for properdisposal. (Refer to Section 2.2.2.4).2.4.2Alkali Spills2.4.2.1 Wear protective gloves and eye protection while pouring dry absorbentmaterial around the outer edge of the spill. Continue to add the dryneutralizing material to the spill area until the liquid has beenabsorbed. The contents of a base neutralization kit, paper towels, orvermiculite may be used to absorb the liquid. NOTE: DO NOT addacids to concentrated or strong caustic spills. There may be violentchemical reactions, causing the spill to spread to a wider area.2.4.2.2 When the liquid has been absorbed, scoop up the absorbing material,and rinse well with water before placing in a proper disposal container.NOTE: Caustics are difficult to wash out of absorbents, so continuethe washing for several minutes.2.4.2.3 After the spilled liquid and absorbents have been removed, pour smallamounts of dilute acetic (2-3%) or 1% hydrochloric acid on the spillarea. Allow neutralization to be completed. While still wearingprotective gloves and eye protection, mop or wipe up the liquid. Rinsethe area again and wipe with a wet mop or towels. Cleanup materialsshould be disposed of in a plastic container (Refer to Section 2.2.2.4).2.5Radioactive Injuries and Spills2.5.1Radiation Accident Involving Injury2.5.1.1 Notify the Radiation Safety Officer (RSO) immediately (443-6813856) concerning injuries or spills.MDH – Laboratories AdministrationLaboratory Safety Manual: 6.1 Ed. 4/20186

2.5.1.2 Restrict access to the area. Provide necessary first aid and notifyappropriate emergency personnel using phone numbers found at thefront of this manual.2.5.1.3 If the person is contaminated and must be transported out of the areafor medical attention, remove contaminated clothing and thoroughlywash skin surface with soap and water with a scrub brush.2.5.1.4 If decontamination is not possible, wrap the person in a blanket tominimize contamination spread. At the medical facility, instructmedical personnel of the need for contamination control and radiationsurveillance.2.5.1.5 If the injury does not require immediate medical care, have the personremove contaminated clothing and wash the affected skin surfaceswith water, followed by mild soap and water. Survey contaminatedskin surfaces after each washing until contamination is reduced to lessthan 100 dpm/100 cm2. (More extensive skin and areadecontamination procedures are outlined in the Safe Handling ofRadioactive Materials Handbook 92 and Guide to Safe Handling ofRadioactive Material (Perkin-Elmer). Both pieces of literature arefound in the Radiation Laboratory. Contact the RSO.)2.5.1.6 Assess the radiation dose, both external and internal (urine assay,thyroid count), received by personnel involved in the accident.Maintain appr

Laboratory Safety Manual rewritten and updated for new facility 1770 Ashland Ave. 2015 : 5.1 Edition . Laboratory Safety Manual reviewed and updated the glove personal protection equipment selection guidelines and Appendix B. 6/15/2016 . 6.0 Edition : Laboratory Safety Manual revised and updated State Maryland's new health care provider .

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