LABORATORY SAFETY MANUAL - City University Of New York
LABORATORY SAFETY MANUAL2018LABORATORY SAFETY MANUALWINTER 2018OFFICE OF ENVIRONMENTAL, HELATH SAFETY AND RISK MANAGEMENT 205 East 42nd Street, NewYork, NY 10017
LABORATORY SAFETY MANUALPUBLIC SAFETY AND 911 IN ANY EMERGENCY THAT REQUIRES IMMEDIATEPOLICE, FIRE, OR MEDICAL RESPONSE TO PRESERVE A LIFE.
LABORATORY SAFETY MANUAL1PURPOSE OF THIS MANUALThe Office of Environmental, Health, Safety and Risk Management, in partnership with theEnvironmental, Health and Safety Officer Council and the Office of the Vice-Chancellor forResearch, has developed this Laboratory Safety Manual to minimize the risks associated withlaboratory activity and ensure that CUNY remains in compliance with the Occupational Safetyand Health Administration (OSHA) regulation 29 CFR 1910.1450, "Occupational Exposure toHazardous Chemicals in Laboratories,” or what is commonly referred to as the “LaboratoryStandard.” 1, 2 The Laboratory Standard requires the development of a Chemical Hygiene Plan(CHP) for each laboratory workplace that protects employees from health hazards associatedwith hazardous chemicals in the laboratory and maintains exposures below OSHA PermissibleExposure Limits. In addition to addressing those regulations that are mandatory, this manualalso offers some best management practices supported by leading standards settingorganizations and research institutions.0F1FThroughout this document, regulatory requirements will be clearly identified using words suchas “must,” “required,” and “shall.” Colleges, departments, other units, and individuallaboratories are free to adopt other non-mandatory guidelines found within this document asapplicable for their units or laboratories.To take advantage of the Internet, this document is formatted to be a “front door” to otherresources, including useful web links in the notes section. For those internal hyperlinks,including the Table of Contents, you can navigate through the document by clicking on the“Back” and “Forward” hyperlink arrow buttons.This Laboratory Safety Manual is not intended to replace or supersede any specific operationalrules or procedures that have been adopted by the University to comply with environmental,health, and safety regulations or policies. It is a dynamic document and will be reviewedperiodically and updated based on the comments and suggestions of readers, laboratory users,and the broader CUNY scientific community.1CUNY EHSRM gratefully acknowledges the generosity of Cornell University’s Office of Environmental Health andSafety for allowing CUNY to use its own Laboratory Safety Manuel as the base for this document.229 CFR 1910.1450. Accessible how document?p id 10106&p table STANDARDS
LABORATORY SAFETY MANUAL2TABLE OF CONTENTS1.0 INTRODUCTION. 111.1 CHEMICAL HYGIENE PLAN (CHP) ACCESSIBILITY . 121.2 LABORATORY SAFETY RESPONSIBILITIES. 131.2.1 NEW YORK CITY LABORATORY REQUIREMENTS. 131.3 ROLES AT CUNY . 152.0 ENGINEERING CONTROLS . 172.1 CHEMICAL FUME HOODS . 172.1.1 PERCHLORIC ACID USE . 182.1.2 RADIOACTIVE MATERIAL USE . 182.1.3 FUME HOOD INSPECTION AND TESTING PROGRAM . 182.1.4 INSTALLATION OF NEW FUME HOODS. 192.1.5 REMOVAL OF EXISTING FUME HOODS . 192.2 OTHER CAPTURE OR CONTAINMENT DEVICES . 192.2.1 GLOVE BOXES . 202.3 WATER PROTECTION IN LABS. 213.0 PERSONAL PROTECTIVE EQUIPMENT . 213.1 LABORATORY PERSONNEL RESPONSIBILITIES . 223.2 TRAINING FOR PERSONAL PROTECTIVE EQUIPMENT . 233.3 EYE PROTECTION . 233.3.1 EYE PROTECTION SELECTION . 233.4 HAND PROTECTION . 253.4.1 SELECTING THE PROPER GLOVES . 253.4.2 DOUBLE GLOVING . 263.4.3 TYPES OF GLOVES . 273.5 PROTECTIVE CLOTHING . 283.6 RESPIRATORS . 293.7 HEARING PROTECTION . 303.8 FOOT PROTECTION . 314.0 ADMINISTRATIVE CONTROLS . 314.1 STANDARD OPERATING PROCEDURES . 324.2 PROCEDURAL CONTROLS . 334.3 HOUSEKEEPING . 33
LABORATORY SAFETY MANUAL34.4 PERSONAL HYGIENE . 344.5 EATING, DRINKING, AND APPLYING COSMETICS IN THE LABORATORY . 354.6 WORKING ALONE . 354.6.1 WORKING ALONE - RESPONSIBILITIES . 374.6.2 BUDDY SYSTEM AND WORKING ALONE . 374.6.3 C-14 REQUIREMENTS FOR THE WRITTEN EXAM . 384.6.4 RENEWAL GUIDELINES FOR CERTIFICATE OF FITNESS. 394.7 SUPERVISION IN THE LABORATORIES - DEFINED . 394.8 PHONE ACCESS . 404.9 UNATTENDED OPERATIONS . 404.10 ACCESS TO LABORATORIES . 414.10.1 GENERAL VISITORS . 414.10.2 VISITING SCIENTISTS AND OTHER SIMILAR USERS . 414.10.3 PETS IN LABS . 414.11 PURCHASING CHEMICALS . 414.12 ORDERING NEW EQUIPMENT . 424.13 WORK ORDERS AND TICKET REQUESTS . 424.14 CHANGES IN LABORATORY OCCUPANCY. 424.15 LABORATORY DESIGN AND CONSTRUCTION . 434.16 VENTILATION RATES . 434.16.1 ROOM AIR PRESSURE IN LABS. 434.17 ENERGY CONSERVATION IN LABORATORIES . 444.18 RESEARCH AREA INSPECTIONS. 444.18.1 SELF-INSPECTIONS . 454.18.2 INSPECTIONS BY REGULATORY AGENCIES . 454.19 LABORATORY SECURITY . 465.0 EMERGENCY PREPAREDNESS . 475.1 EMERGENCY PROCEDURES . 475.2.1 EMERGENCY EVACUATION PROCEDURES . 485.2.2 LABORATORY EMERGENCY SHUTDOWN PROCEDURES . 485.2.3 MEDICAL EMERGENCY PROCEDURES. 495.2.4 FIRST AID KITS . 495.2.5 FIRE OR EXPLOSION EMERGENCY PROCEDURES. 505.2.6 FIRE EXTINGUISHERS . 515.2.7 POWER OUTAGE PROCEDURES . 525.3 CHEMICAL SPILL PROCEDURES. 525.3.1 INCIDENTAL SPILLS . 525.3.2 SPILL ABSORBENT MATERIALS . 535.3.3 SPILL KITS. 545.3.4 MAJOR SPILLS . 555.4 EMERGENCY EYEWASH AND SHOWERS . 565.4.1 TESTING AND INSPECTION OF EMERGENCY EYEWASH AND SHOWERS . 57
LABORATORY SAFETY MANUAL45.4.2 INSTALLATION OF NEW EMERGENCY EYEWASH STATIONS AND SHOWERS . 575.4.3 USING EMERGENCY EYEWASH AND SHOWERS . 585.5 INJURY/ILLNESS REPORTING . 595.6 MEDICAL CONSULTATIONS . 595.6.1 INFORMATION PROVIDED TO THE PHYSICIAN . 595.6.2 THE PHYSICIAN’S WRITTEN OPINION . 606.0 EMPLOYEE INFORMATION AND TRAINING . 606.1 TRAINING OPTIONS . 617.0 SAFE CHEMICAL USE . 627.1 MINIMIZE EXPOSURE TO CHEMICALS . 627.2 UNDERSTANDING CHEMICAL HAZARDS . 637.2.1 CHEMICAL HAZARD INFORMATION . 647.3 SAFETY DATA SHEETS (SDSS) . 647.3.1 SDSS AND NEWLY SYNTHESIZED CHEMICALS . 657.4 ROUTES OF CHEMICAL ENTRY . 657.4.1 INHALATION . 667.4.2 INGESTION . 667.4.3 INJECTION . 677.4.4 EYE AND SKIN ABSORPTION . 677.5 CHEMICAL EXPOSURE LIMITS . 687.6 CHEMICAL EXPOSURE MONITORING . 697.7 TOXICITY . 697.7.1 TOXIC EFFECTS . 707.7.2 EVALUATING TOXICITY DATA . 707.8 CHEMICAL LABELING. 717.8.1 LABELING NON-ORIGINAL CONTAINERS FOR CONTENT . 717.8.2 LABELING NON-ORIGINAL CONTAINERS FOR HAZARDS . 717.9 CHEMICAL STORAGE . 727.9.1 GENERAL STORAGE GUIDELINES . 737.9.2 CHEMICAL STORAGE LIMITS . 747.10 TRANSPORTING CHEMICALS . 748.0 CHEMICAL HAZARDS . 758.1 EXPLOSIVES . 758.2 FLAMMABLE AND COMBUSTIBLE LIQUIDS . 778.2.1 FLAMMABLE STORAGE IN REFRIGERATORS/FREEZERS . 788.2.2 FLAMMABLE STORAGE CABINETS . 798.3 FLAMMABLE SOLIDS . 79
LABORATORY SAFETY MANUAL58.4 SPONTANEOUSLY COMBUSTIBLE . 808.5 DANGEROUS WHEN WET . 808.6 OXIDIZERS AND ORGANIC PEROXIDES . 808.7 PEROXIDE FORMING COMPOUNDS . 818.8 POISONS. 838.9 CORROSIVES . 848.9.1 HYDROFLUORIC ACID . 858.9.2 PERCHLORIC ACID . 879.0 PARTICULARLY HAZARDOUS SUBSTANCES . 879.19.29.39.49.59.69.79.8ESTABLISHMENT OF A DESIGNATED AREA . 88SAFE REMOVAL OF CONTAMINATED MATERIALS AND WASTE . 88DECONTAMINATION PROCEDURES . 88GUIDELINES FOR WORKING WITH PARTICULARLY HAZARDOUS SUBSTANCES. 88PRIOR APPROVAL . 90SELECT CARCINOGENS . 90REPRODUCTIVE TOXINS . 92ACUTE TOXINS . 9210.0 HAZARDOUS CHEMICAL WASTE DISPOSAL . 9311.0 SHIPPING HAZARDOUS MATERIAL. 9411.1 REGULATED HAZARDOUS MATERIALS . 9411.2 HAZARDOUS MATERIALS TRANSPORTATION REQUIREMENTS . 9511.3 DRY ICE REQUIREMENTS . 9511.3.1 PACKAGING DRY ICE . 9512.0 PESTICIDES . 9712.1 PESTICIDE CERTIFICATION. 9712.1.1 EXEMPTIONS FROM PESTICIDE CERTIFICATION . 9713.0 BIOHAZARDS . 9813.1 INSTITUTIONAL BIOSAFETY COMMITTEE. 9813.1.1 RECOMBINANT DNA. 9813.1.2 INFECTIOUS AND PATHOGENIC AGENTS . 9913.1.3 BLOODBORNE PATHOGENS . 9913.1.3.1 COLLABORATIVE INSTITUTIONAL TRANING INITIATIVE (CITI) . 9913.1.4 SELECT BIOLOGICAL AGENTS AND TOXINS . 100
LABORATORY SAFETY MANUAL613.2 ANIMAL USE . 10013.2.1 CITI (ANIMAL BIOSAFETY)70F . 10113.3 HUMAN PARTICIPANTS. 10113.4 SHIPPING BIOLOGICAL MATERIALS . 10113.4.1 PERMITS FOR THE IMPORT AND EXPORT OF BIOLOGICAL MATERIALS . 10213.5 BIOLOGICAL SAFETY CABINETS . 10513.5.1 BIOLOGICAL SAFETY CABINET CERTIFICATIONS . 10513.5.2 WORK PRACTICES AND PROCEDURES . 10513.5.3 BSC OPERATIONAL PROCEDURES . 10613.5.4 USE OF ULTRAVIOLET LIGHTS IN THE BSC . 10713.5.5 TYPES OF BIOLOGICAL SAFETY CABINETS. 10713.6 BIOHAZARDOUS WASTE (REGULATED MEDICAL WASTE) . 10913.6.1 HYPODERMIC SYRINGES AND NEEDLES . 10914.0 RADIATION HAZARDS . 11014.1 WHERE IONIZING RADIATION IS USED . 11014.2 CONTROL OF IONIZING RADIATION . 11014.3 POTENTIAL HAZARDS . 11114.3.1 HOW TO PROTECT YOURSELF . 11114.4 RADIOACTIVE WASTE DISPOSAL . 11215.0 LASER HAZARDS . 11216.0 PHYSICAL HAZARDS . 11416.1 ELECTRICAL SAFETY . 11416.1.1 COMMON ELECTRICAL HAZARDS AND PREVENTATIVE STEPS . 11516.1.2 SAFE USE OF ELECTROPHORESIS EQUIPMENT . 11816.2 MACHINE HAZARD . 11916.2.1 MACHINE SAFETY RESPONSIBILITIES . 12016.2.2 MACHINE GUARDING . 12016.2.3 PERSONAL PROTECTIVE EQUIPMENT . 12116.2.4 COMMON MACHINE HAZARDS . 12116.3 LIGHTING . 12316.4 COMPRESSED GASES. 12316.4.1 HANDLING COMPRESSED GAS CYLINDERS . 12316.4.2 SAFE STORAGE OF COMPRESSED GAS CYLINDERS . 12416.4.3 OPERATION OF COMPRESSED GAS CYLINDERS . 12516.4.4 RETURN OF CYLINDERS . 12616.4.5 HAZARDS OF SPECIFIC GASES . 12616.5 BATTERY CHARGING . 12816.6 HEAT AND HEATING DEVICES . 129
LABORATORY SAFETY MANUAL716.6.1 HEAT STRESS . 13016.7 COLD TRAPS . 13016.8 AUTOCLAVES . 13116.9 CENTRIFUGES . 13216.9.1CENTRIFUGE ROTOR CARE. 13216.10 CRYOGENIC SAFETY . 13316.10.1 CRYOGENIC SAFETY GUIDELINES . 13416.10.2 CRYOGENIC CHEMICAL SPECIFIC INFORMATION . 13616.11 EXTRACTIONS AND DISTILLATIONS . 13716.12 GLASS UNDER VACUUM . 13816.13 WASHING GLASSWARE . 13816.14 GENERAL EQUIPMENT SET UP .
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