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MITOSIS & MEIOSISBio Review

MITOSISPart 1

THE CELL CYCLE AND MITOSISDue to the loss and death of cells must replace them.How many cells in your body? 50-100 million trillion Every minute your body produces about 300 million new cellsThree stages in the cell cycle:1. Interphase: cell carries out normal functions.2. Mitosis: nucleus contents duplicated and divided intotwo equal parts.3. Cytokinesis: separation of two nuclei and cell contentsinto two daughter cells.

PARTS OF THE CELL CYCLE

INTERPHASEInterphase - the longest cell cycle stage (lasts 15 hrs. – months). cell performs normal functions and grows.In late interphase, DNA copies itself in the process ofreplication.Replication involves several steps:1. The DNA molecule unwinds with the help of an enzyme.2. New bases pair with the bases on the original DNA.3. Two new identical DNA molecules are produced. Now, double the amount of DNA in nucleus it divides (Mitosis)

DNA UNRAVELING (VIDEO)

LATE INTERPHASE CONT’D.Chromatin is in its loosely coiledform so that DNA can be copiedinto RNA for proteins to be made inpreparation for cell division.At the end of interphase, the cellcontinues to grow and makeproteins in preparation for mitosisand cytokinesis.

CHROMOSOMESAs the nucleus prepares to divide,replicated DNA in interphase joins to formsister chromatids, joined by a centromere.

MITOSIS OVERVIEW:Mitosis is the shortest stage of the cell cycle where thenuclear contents divide, and two daughter nuclei areformed.It occurs in 4 stages:1.2.3.4. ProphaseMetaphaseAnaphaseTelophaseHelpful saying to remember the order:“I picked many apples today.”

MITOSIS - PROPHASEChromosomes start to coil andbecome visible.Pairs of centrioles start toseparate.The nuclear membranedisappears.Spindle fibers start to formbetween the centriole pairs.Chromosomes move moreevenly throughout the nucleus.

MITOSIS - METAPHASECentriole pairs move toopposite ends of the cell.Spindle fibers are stillattached to the centriolepairs.Chromosomes line up alongthe midline of the cell andare attached to the spindlefibers.

MITOSIS - ANAPHASEThe pair of chromatids split atthe centromere and move toopposite ends of the spindle.Now there are twice the numberof chromosomes within the cellmembrane.Movement of the chromosomestowards the opposite ends ofthe cell membrane is aided bythe spindle fibers.

MITOSIS - TELOPHASENuclear membranes formaround the two new sets ofchromosomes.The spindle fiber disappears.Chromosomes start to uncoil(chromatin) and become lessvisible.Cell starts to make a groove(furrow) in the middle toeventually split into twoidentical cells.

CYTOKINESISThe division of material outsideof the nucleus. Occurs after telophase.Divides the organelles and othersubstances in the cytoplasm intoroughly two equal halves.Animal cells furrow while plantcells form a cell plate# chromosomes in daughter cell the # chromosomes in parentcell.Daughter cells are geneticallyidentical to parent.Video on Mitosis

MEIOSISPart 2

OBJECTIVESBy the end of the lesson you should be able to:Describe the process of meiosisCompare and contrast meiosis and mitosisExplain why meiosis is needed

MEIOSISSexual reproduction – 2 parents – genetic differencesThe process of Meiosis results in the production of special cells calledgametesGametes have half the number of chromosomes as body cells

MEIOSISCell division occurs twice in Meiosis Once at the end of Meiosis I and again at the end of Meiosis IIIn Meiosis I, matching chromosomes are called homologous chromosomesseparateIn Meiosis II, sister chromatids separate

MEIOSISThe process of meiosis shuffles genetic information and results invariation of the gametesGenetic diversity

MEIOSISAKA: Germ cell divisionPurpose: to producegametes (i.e. sex cells:sperm and egg)Products: 4 haploid cellseach genetically different this creates geneticvariability

TERMS:Haploid: half the total number of chromosomes found in gametes (inhumans 23)Diploid: total number of chromosomes found in body cells (in humans 46)

FERTILIZATION2 gametes come together; in humans an egg and sperm cometogetherEach has 23 chromosomes, thus together there is 46 in the zygote23 4623Gametes: why is thesperm smaller than theegg?Zygote: will grow (bymitosis) into a baby in 9 months

MEIOSIS: REDUCING CHROMOSOME NUMBER

NUMBER OF CHROMOSOMES SEQUENCE46Parent Cell924646232323All genetically different23DaughterCells

CHROMOSOME SEQUENCEMeiosis VideoMeiosis 2 Video

MEIOSIS – PROPHASE IChromosomes coil andbecome visible.Nuclear membranedisappears.Spindle fibers form.Homologouschromosomes movetowards each other andpair.

MEIOSIS – METAPHASE IPairs of homologouschromosomes move to themidline of the cell.These pairs are alignedon either side of themetaphase plate.

MEIOSIS – ANAPHASE IHomologous chromosomepairs are pulled awayfrom each other towardsopposite ends of the cell.Paired chromatidsremain attached.

MEIOSIS - TELOPHASE IPaired chromatids mayuncoil and become lessvisible.Nuclear membranes mayform around eachchromosome cluster.Spindle fibers disappear.Cytokinesis occurs.

MEIOSIS IIPhase II of meiosis is the mitotic division of haploid cells.Although there will be four new non-identical cells at the end ofmeiosis II, all other processes for this division are similar to mitosis.

MEIOSIS II – PROPHASE IIPaired chromatids coil.Spindle fibers form.Nuclear membranedisappears.

MEIOSIS II – METAPHASE IIPaired chromatids lineup in the middle of thecell.The chromatids areattached to spindlefibers.

MEIOSIS II – ANAPHASE IIEach pair of chromatidssplit at the centromere toform two independentchromosomes.Chromosomes move toopposite ends of the cell.

MEIOSIS II – TELOPHASE IIChromosomes uncoil andbecome less visible.Nuclear membranes fromaround four new nuclei.Spindle fibers disappear.Cytokinesis occurs.Meiosis Square Dance

MEIOSIS - CYTOKINESISSame process as in mitosis.Four non-identical cells areformed.These cells are calledhaploid cells (n).# chromosomes half the# of chromosomes ofparent cell.

MEIOSIS EVENTSMeiosis IMatching chromosome pairs(homologous chromosomes )move to opposite poles of the cell two daughter cells result.Meiosis IIChromatids of each chromosomeare pulled apart - the end result isfour haploid cells, each with halfthe number of chromosomes. Thesedevelop into gametes.

CROSSING OVERImportant event that occurs between each chromosome pair in meiosis IThere is an exchange of segments of DNA and picks up new geneticinformation

INDEPENDENT ASSORTMENTThis occurs when homologous pairs of chromosomes separate at theequator and move towards opposite poles of the cell.VideoMitosisvs Meiosis

FERTILIZATIONThere are more than 8 million combinations possible for these 23 pairsin any egg or sperm cellWhen fertilization occurs, 70 trillion different zygotes are possiblefrom the combination of one sperm cell and one egg cell!

GAMETE FORMATIONMales produce 4 sperm for every round of meiosisFemales produce one egg for each round of meiosis: 3 of the 4 “eggs”produced die off - called polar bodiesAll the cytoplasm, nutrients and energy gets put into one egg

Mitosis vs Meiosis. FERTILIZATION There are more than 8 million combinations possible for these 23 pairs in any egg or sperm cell When fertilization occurs, 70 trillion different zygotes are possible from the combination of one sperm cell and one egg cell! GAMETE FORMATION

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