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Lab Safety SeminarAlphabet SoupEH&S, IIPP, EAP, SOP, CHP, LSP, PI,LHAT, PPE, SDS, CIS, CUPA, BUA,RUA, JSA (JHA)Department of Food Science and TechnologyUniversity of California, Davis

General Laboratory Safety“Don’ts”lDon’t eat, drink, chew gum or apply cosmetics wherechemicals or biological hazards are usedlDon’t store food in refrigerators or cold rooms withchemicals or other hazardous materialslDon’t leave equipment or reactions to run unattendedlDon’t work alone in the lab after normal working hourswithout supervisor’s approval

Don’t leave a mess

Safety Seminar TopicsInjury and Illness Prevention Plan (IIPP) Department Chemical Hygiene Plan (CHP) UC Davis Laboratory Safety Manual Lab Hazard Analysis (LHAT), Standard Operating Procedures (SOP) PI Training Online UC Safety Training (UC Fundamentals of Laboratory Safety) UCOPSite Specific Training Checklist (Site-Specific Safety Orientation & Training for NewLaboratory Personnel) PI (Principle investigator)Chemical Inventory System (Chemicals) PISafety Data Sheet (SDS) Global Harmonized System (GHS)Personal Protection Equipment (PPE) PIEmergency Action Plan (EAP) DepartmentHazardous Materials - Handling and Disposal: Lab Standard or HazardCommunication (HazCom) PI with EH&SSafety Services Resources - http://safetyservices.ucdavis.edu/

Injury and Illness Prevention Plan (IIPP) Management commitment/assignment of responsibilitiesSafety communications system with employeesSystem for assuring employee compliance with safe workpracticesScheduled inspections/evaluation systemProcedures for correcting unsafe/unhealthy conditionsSafety and health training and instructionRecordkeeping and documentationAccident Investigation

ELEMENTS OF THE IIPP

Campus Chemical Hygiene Plan

Chemical Hygiene Plan (CHP) Establishes a formal written program for managing the risksposed by health and safety hazards associated with the use ofhazardous chemicals in laboratoriesDescribes the proper use, handling, storage and disposalpractices and procedures to be followedApplies to employees who use chemicals in teaching andresearch laboratories at the UC Davis CampusEmployer: Shall provide a workplace free from recognizedhazards that may cause death or serious injuryEmployee: Shall comply with occupational safety and healthstandards, rules, regulations, and orders.

Chemical Hygiene Plan (CHP) Rights and Responsibilities (UC Office of the President and Board of Regents)– Campus Administration Policies and Procedures (Chancellor,Provost and Deans Offices)– Policy Specifics (Chemical and Laboratory Safety Committee)– PI Support and Enforcement (Department Chair)– Site Specific Rules, Training, and Laboratory SafetyProcedures (PI)– Training, Standard Operating Procedures, Safety Data Sheets,Personal Protective Equipment (Personnel)– Information, Training Tools, Support, Inspections (EH&S)– Laboratory Safety boratory-safety-manual

Online Toolsehs.ucop.edu/

Hazard Assessment (LHAT)

Laboratory Safety Training Check Listq Take the online UC Laboratory Safety Fundamentalsq Complete site specific training, sign and dateq Complete any additional training assigned by your supervisor (TrainingMatrix)q Review LHAT and view PPE videoq Obtain Personal Protective Equipment (PPE) as determined by the LHATq Receive training on conducting “Standard Operating Procedure” labfunctions or “Prior Approval” procedures (specific reactions & equipment)q Read and understand the IIPP, Emergency Action Plan (EAP) andChemical Hygiene Plan (CHP)

UC Laboratory Safety FundamentalsUC Laboratory Safety FundamentalsThis on-line course must be successfully completedby all existing laboratory personnel before any newworker is granted unescorted access to the laboratoryIntroduction to laboratory safety fundamentals, chemicalsafety, and general safety. This course covers relevantcampus Laboratory Safety Manual(s) andrights/responsibilities according to applicable regulations

Site SpecificTraining

TrainingMatrix

LMS Safety Training Classes andEH&S Safety Nets Research and Laboratory Safety General and Equipment Safety Ergonomics Biological Safety – BUA Animal Care

Laboratory Chemical Use Check Listq Check lab chemical inventory before ordering a chemicalq Add each new chemicals to chemical inventory with barcode tag(ChemTag)q Read Safety Data Sheet (SDS)q Add to the Standard Operating Procedure (SOP) or create a newSOP if requiredq Use proper personal protection (long pants, sleeved shirt, closedtoe shoes, eye protection, lab coat, proper gloves, etc.)q *Dispose of chemical waste in properly labeled and dated container*WASTe online system, SafetyNet 8

Chemical Inventory System (Chemicals)

SDS InformationlIdentity of the chemicallHazardous nature of chemicallPhysical characteristic (e.g., boiling point)lFire and explosion informationlReactivity datalHealth hazard data (e.g., health effects, symptoms)lPersonal protective equipment neededlHow to handle leaks, spills and disposallSpecial precautions

SDSInformation

The Globally Harmonized System of Classificationand Labeling of Chemicals(GHS) Signal word – either DANGER or WARNING Precautionary statement indicating product handling to minimize risks to the userH200 Physical HazardH300 Health HazardH400 Environmental HazardThe lower the number within the category thehigher the hazard i.e. H300 is more hazardousthan H304

Hazard SymbolsDangerous tothe ressurized GasFlammableLong TermHealth Risk

Hazardous Material Control SystemslChemical Fume HoodslGlove BoxeslFlammable Liquid Storage CabinetslBiological Safety CabinetslChemical Spill Clean-up KitlOther Engineering Controls

Standard Operating Procedures Document the laboratory-specific procedures for the safehandling, storage and disposal of hazardous chemicals– PIs and laboratory supervisors are responsible forestablishing SOPs relevant to health and safety forlaboratory activities involving hazardous chemicals undertheir direction Cal/OSHA requires standard operating procedures (SOPs) beestablished for work with hazardous chemicals– Stated in the 8 CCR §5191(Occupational Exposure toHazardous Chemicals in Laboratories, “LaboratoryStandard”) under the provisions of the Chemical HygienePlan

Elements of an SOP Establish a designated work and storage area Determine engineering controls, i.e. fume hood Determine proper personal protective equipment Establish procedures for waste removal Set up decontamination procedures

Hazard Class SOP Acutely Toxic ChemicalsCarcinogensCorrosivesCryogensFlammable Solids and LiquidsReproductive ToxinsPyrophoricsWorking AloneWater reactivesPotentially Explosive CompoundsTemplates available on Safety Services website

Personal Safety

Personal Protective Equipment (PPE)lEye Protection— Safety Glasses, Safety Goggles, FaceShieldslGloves— Nitrile, Chemical-handling, High-temp.Lo-temp.lOther Protective Clothing— Lab Coats, Aprons, etc.lRespiratory Protection— Dust and Mist RespiratorslOther— e.g., Hearing Protection

PPE Etiquette Do not wear soiled or contaminated lab coats in shared spaces– Wear clean lab coats for use in autoclave rooms Transport items in clean secondary containers and do not use gloves inhallways.– If transporting large amounts of liquids (1 L or more) use a clean cart to transport items(still must be stored in clean secondary containers) If working in another lab, have another lab coat in that lab OR bring a cleanlab coat with youRemove gloves before putting on or taking off lab coat.Change gloves immediately if any chemical or biohazard is spilled on them– Gloves have breakthrough time for certain chemicals and can have small holes unseen tothe naked eye (1-5 out of 80 gloves can have “allowable” defects). Do not touch face, phones or any exposed body part or personal items withglovesWash hands after removing lab coat

Glove selection and breakthrough icalAcetic acidFormic acid223344231242Hydrofluoric acid(up to 70%)22311?Nitric acid 70 %?12?14Sulfuric acid 70 %BenzeneTolueneXylene111121114111211111112344

Emergency Action Plan (EAP)The program must be in writing and include the following elements:– Emergency escape procedures and emergency escape routeassignments– Procedures to account for all employees after an emergency evacuation– The preferred means of reporting fires and other emergencies– Names or regular job titles of persons or departments who can becontacted for further information or explanation of duties under the plan– A system to notify employees of an emergency– Procedures for employees who remain to complete critical operationsbefore they evacuate– Rescue and medical duties for those employees whoare to perform them– Training for all employees on the EAP– The written plan must be kept in the workplace andmade available for employee review

Emergency Action PlanKnow the locations of:lAll exits for your workplace and the buildinglAlarm pull boxes and fire extinguisherslNearest phonelSafety showers and eyewasheslFirst-aid kitslChemical spill kitsIf you will need assistance during evacuation, please contactme in advance!

Evacuation Plan - RMI South - 2nd Floor

NWESEMERGENCYEVACUATIONMAPOld Davis RoadRMISensoryRMI NorthWalkwayDEPT. OF VIT. & ENOLOGYMEETING TMENT OF FOODSCIENCE & TECHNOLOGYMEETING AREABVTFAppendix III

Procedures in Case of FirelIf fire is small you may attempt to neutralize the threatwithout endangering yourselflIf you are unsure - Leave the area, being sure others areoutlCLOSE THE DOOR!lACTIVATE THE NEAREST BUILDING FIRE ALARMlOnce manual pull alarm is activated, alarm will emitaudible and visual (strobing lights) signals to warnoccupants of evacuationlDIAL 911 (or 530-752-1230)lSTAY AWAY FROM AREA AND CLOSE ALL DOORS!lDoors in RMI complex are fire rated and will helpprevent the spread of fire to surrounding areaslGo to agreed meeting placelStand by to advise the emergency personnel when theyarrive

Fire extinguishers: Types and how touse 5 types– Class A: ordinary combustibles wood, paper,trash etc– Class B: flammable/combustible liquids– Class C: Electrical fires– Class D: special metals– Class K: Kitchen fires (oil, grease, etc.)All buildings in RMI equipped with comboextinguishers (A B C)Emit a powder that suffocates the fireNeed to be checked monthly

How to check fire extinguishers Need to be checked monthly Make sure needle is within thegreen range– Look at the needle as if youwere looking at a meniscus ofa liquid Put date and initials of personchecking on the tag If there are issues, contactfireprevention@ucdavis.edu

How to use a fire extinguisherPASS– P: pull the pin (note take care notto squeeze handle)– A: aim at the base of the fire andstand back 5-6 ft– S: squeeze the handle and movecarefully closer as powder is beingdischarged– S: sweep back and forth to coverthe range of the fire This should only be done when fireis at the beginning stage. If flamesare large, evacuate. 911 shouldalready have been alerted Fire extinguisher will only last 20-30secondsFire Extinguisher Training VideoOSHA

Procedures in Case of EarthquakelGet under a desk, table, archway, etc. during the shakinglLeave the building after the shaking is overlIf outside during shaking, stay clear of buildings, trees, etc.lDIAL 911 (or 530-752-1230) to report any fires, rupturedpipes or downed electric lineslAssist injured persons in securing medical attentionlGo to agreed meeting placelStand by to advise emergency personnel when they arrive

Active Shooter Immediate actions– Run– Hide– FightSeek Help– Call 911 and provide as much information as possible: Location, what is happening, description of suspect,your name and numberSilence your phone:– If you cannot speak, turn the sound off with line openso police can hear what is going cypreparedness/procedures/active-shooterLMS: Shots Fired

Campus Safety TipsSafety tips from UCD Police Department: Always be alert to your surroundings. This includes persons and vehicles. Report suspicious activity to UC Davis Police at (530) 754-2677. Do not allow strangers to enter behind you at secured facilities, residences halls and donot lend your entry keys. Report criminal activity (such as break-in, intruder, robbery, assault) immediately to 9-11 Always lock your doors. Secure your property by locking doors to offices, laboratories,and buildings. At campus office facilities, report malfunctioning or broken doors and windows toFacilities call (530) 752-1655Department Safety Tips: Do not leave personal items or items of value in your vehicles and always lock yourvehicle, Set up a communication system with other co-workers. If working late or alone, this canbe used to alert of your safe arrival and departure from the building and in case ofemergencies. Make sure all entry doors are closed and not propped open to prevent building breakins and theft. Report any building malfunctions to bftvfacilities@ucdavis.edu

Campus Resources for Personal or PropertySafetyUC Davis Campus Emergency Line: (530) 752-1230 For Police, Fire, or Medical emergencies If on a cell phone on campus, use this number, for landline, dial 911UC Davis Police Department 24 hour Non-Emergency: (530) 752-1727 Can be contacted to report suspicious behavior or for non-emergency situations Can be contacted if you are on Campus and feel unsafeSafe Rides/UCD Police Department: (530) 754-2677 https://police.ucdavis.edu/safe-rides Hours of Operation:– 7 days a week, 5:00 p.m. to 10:00 p.m., on-campus to another on-campus location– 7 Days a week 10:00 p.m. to 6:00 a.m., on-campus location pick-up to any locationin the city of Davis– VIP wheelchair transportation 24/7 on campus to another on campus location 7 Days a week, 10:00 p.m. to 6:00 a.m., on-campus location pick-up to anylocation in the city of Davis

Emergency notificationCampus emergency notificationUCD WarnMe alertsEverbridge- personal safety mobile app

Contributors to accidents Rushing, Frustration, Fatigue, Complacency Leads to Errors:Eyes not on taskMind not on taskLine-of-fireBalance/Traction/Grip

Procedures in Case of Chemical Spill 1 pint or more or when in doubt, call UC Davis Fire Department (911)Evacuate the room, close the door, and wait for emergency personnelFlammable? Turn off all ignition sources before securing the roomIn case of chemical contact with skin or eyes, flood the affected areaimmediately with water; Seek medical assistanceAll contaminated clothing must be removed immediatelySmall spills (1 pint or less) may be cleaned up by laboratory personnelwith a spill kitAcids and bases should be absorbed and neutralized– Flammable liquids may be absorbed– DO NOT attempt to blot cryogenic liquid spills with unprotected hands, allowthe liquid to evaporate– Solid spills are not usually emergencies. If the material spilled is toxic, usedampened cloths or paper towels to transfer it to plastic bags and disposed ofas hazardous waste.–– Report spills to EH&S (530) 752-1493

Safety Shower and EyewashProcedure If someone is contaminated with hazardous chemicalRemove contaminated clothing if possibleRinse in emergency shower 15 minutesIf eyes are involved, rinse eyes in the eyewash for 15 minutes holding eye(s) openCall 911 or (530)752-1230 or go to thehospital emergency room

Spill KitGuidelines for Chemical Spill ControlSafetyNet #13:General Steps To Follow

Steps for Clean UpWork from the outsideRemove Physical HazardsDispose hazardous wasteAbsorb excess liquid

Reporting a “Near Miss”

Report a “Near Miss” to Safety Services Report an Incident or ConcernAll faculty members, staff, students and visitors at UCDavis can participate in making the campus a safeplace to work, study, and live by identifying healthand/or safety hazards or unsafe conditions byinforming those responsible for the problem area.Employees are advised that use of this form orother reports of unsafe conditions or practices areprotected by law. It would be illegal for theemployer to take any action against an employeein reprisal for exercising rights to participate incommunications involving safety.

Definition of Hazardous WastelToxic– Any substance which may be harmful to the environment or hazardous toyour health if inhaled, ingested or absorbed through the skin.– Includes acute toxins, carcinogens, other chronic toxins with bioaccumulative properties or persistence in environmentlReactive– Substances that can produce toxic gases, are explosive, react violentlywith water, or contain cyanide or sulfide– Includes explosives, oxidizers, reducers, water sensitive, acid sensitive, airsensitive and unstable chemicalslFlammable– Flash point 140 F (60 C)– Capable of causing fire through friction, moisture or reactivity– Includes oxidizers and flammable compressed gaseslCorrosive– pH 2 or pH 12.5– Corrosive to tissue or metals

Guidelines for Disposal ofChemical Waste SafetyNet #: 8WASTe program required– All hazardous material and hazardous chemicalwaste must be picked up by EnvironmentalHealth and Safety (EH&S) or an EH&S-approvedcontractor.Drain Disposal– Drain disposal of non-hazardous materials isstrictly regulated. See Safety Net #6 “Can ThisGo Down the Drain?” for more information on theLocal Limits Program.

Hazardous Waste DisposallllllllReduce volume of source and minimize generation of wasteDesignate a lab location in which to store hazardous waste fordisposalUse “Hazardous Waste” label supplied by WASTeUse screw-capped leak-proof container for liquidsKeep bottled liquid waste in secondary container (e.g., lab tray)Segregate waste by hazard class (Stanford segregation guide)Arrange for pickup within 9 months* of initial label datellTriple-rinse empty containers before disposal in trashll*90 days required for some hazardous chemicals*Some empty containers may require pick up by EH&SDispose of syringes, glass pipettes and other sharps materialin specially-designed rigid container

: Waste tracking

Most Frequently Violated Safety RuleslHazardous wastes must be properly managedlLabeling hazardous solutions— The full contents spelled out in English not chemical formulas— Initials of researcher and date of preparationlLab workers must wear protective clothing, minimumclosed-toe and heel shoes and long pants or skirtlWork with acids, bases, solvents, powders, pressure orvacuum requires lab coat or apron and eye protectionlLab workers must be trained on all safety equipment andstandard operating procedures (SOP)lLabs must be “clean” and “without clutter” and no food ordrink allowed

Vanessa Liebermanvmmorales@ucdavis.edu

General Laboratory Safety "Don'ts" l Don't eat, drink, chew gum or apply cosmetics where chemicals or biological hazards are used l Don't store food in refrigerators or cold rooms with chemicals or other hazardous materials l Don't leave equipment or reactions to run unattended l Don't work alone in the lab after normal working hours without supervisor's approval

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