SpectraMax Multi-Mode Microplate Readers Rev D - LabRepCo, LLC

SpectraMax Multi-Mode Microplate ReadersYour applications, your modes, your choice upgradeable platformfor changing lab needs three-mode cuvette portFOR ASSAY DEVELOPMENT Dual monochromatortunability AUTOMATED ABSORBANCEPATHLENGTH CORRECTION Endpoint, kinetic,spectral and wellscanning read types Comprehensive DataAnalysis with SoftMaxPro Software Validation & compliance Robotics compatibilityThe SpectraMax M3, M4, M5 and M5eMulti-Mode Microplate Readers are a modular,upgradeable dual-monochromator microplatereader platform offering a wide range of highperformance multi-mode capabilities ideal for lifescience research and drug discovery screening.Choose from a three- (M3), four- (M4), orfive- (M5/M5e) mode reader customized toyour specific applications or budgetary needs,while optional capabilities allow you to upgradewith other detection modes at a later time. Allconfigurations offer a triple-mode cuvette port,accurate temperature control, microplate shakingand comprehensive data management usingour SoftMax Pro Software. Detection modesinclude: UV-Visible Absorbance (Abs) Fluorescence Intensity (FI) Luminescence (Lum) Time-Resolved Fluorescence (TRF) Fluorescence Polarization (FP)The SpectraMax M5e Reader offers the additionalbenefit of being certified for Cisbio Bioassays’HTRF Assays.Dual Monochromators for assay flexibilityWith SpectraMax Multi-Mode Readers, there isno need to utilize expensive filters to optimizedetection levels and background. The opticalsystems use two scanning monochromators sothe user can determine optimal excitation andemission settings, resulting in assay performancesimilar to that of dedicated single-mode readers.Patented PATHLENGTH CORRECTION FORBETTER ABSORBANCE ACCURACYOnly Molecular Devices microplate plate readersoffer the capability to measure the depth (opticalpathlength) of samples with no termperaturedependency using the patented PathCheck Sensor technology. With SoftMax Pro Software,the PathCheck Sensor automatically normalizesthe well absorbance. This eliminates the need forstandard curves, and, for compounds with knownabsorptive properties, enables users to calculateconcentrations directly from absorbance.

Superior Optics for Optimal Assay PerformanceTop Read HeadFrom ExcitationMonochromatorFL, TRF, FP ExcitationReference DiodeFL, TRF, FP, Lum EmissionTo EmissionMonochromatorand PMTMirrorAbsBeamsplitter andExcitation PolarizerEmission MirrorEmission PolarizersAbsorbance Detector DiodeMicroplateEmission MirrorBeamsplitterBeamsplitterFL, TRF, FP ExcitationExcitation MirrorFromExcitationMonochromatorFL, TRF, FP, Lum EmissionReference DiodeAbsBottom Read HeadUNIQUE OPTICAL CHARAcTERISTICSAssay collaboration FOR EASE OF SETUP1. The reference diodes enable elimination ofmeasurement noise due to slight fluctuationsin excitation light intensity.Molecular Devices has collaborated withvarious assay partners to optimize andvalidate homogeneous and heterogeneousbiochemical- or cell-based assay performanceon the SpectraMax platform. To support theseassays, we provide application notes as wellas ready-to-run protocols in our SoftMax ProSoftware. Some of our featured partner assaysinclude HTRF assays from Cisbio Bioassaysand LanthaScreen TR-FRET assays fromInvitrogen.2. The angled emission beam improves signalto-noise,especially in narrow Stokes shiftfluorophores, by reducing stray light.3. Elliptical mirrors are used instead of lensesfor maximum transmission with minimalwavelength distortion.4. Top-quality UV-grade fibers give the highestlight transmission down to even the lowestwavelengths.Reference DiodeFrom ExcitationMonochromatorIMAPPLATFORM VALIDATEDTo EmissionMonochromatorand PMT

Which SpectraMax Microplate Reader Do You Need?SpectraMax M3ReaderSpectraMax M4ReaderSpectraMax M5ReaderSpectraMax M5eReader TRF,HTRF,FPHTRF, FPHTRFN/A Detection ModesAbsorbanceFluorescence IntensityLuminescenceTime-Resolved FluorescenceFluorescence PolarizationHTRFUpgrade OptionsPlate Formats6-, 12-, 24-, 48-, 96-, 384-Well MicroplatesCertification & ValidationIMAP Validation (TR-FRET only)(TR-FRET/FP)(TR-FRET/FP) HTRF CertificationLanthaScreen CertificationKey ApplicationsELISAsDNA/RNA QuantitationProtein AssaysEnzyme KineticsProtease AssaysFluorescent Proteins and FRETCell Viability and Cytotoxicity AssaysReporter Gene AssaysCell Migration AssaysQBT Fatty Acid Uptake AssayNeurotransmitter Transporter Uptake AssayADME-ToxMembrane Permeability

Software for Data Aquisition and AnalysisPowerful, Easy-to-Use SoftwareSoftMax Pro Software features easy-to-set-up protocols for data acquisition, customizablespreadsheet functionality for analysis, and powerful graphing tools for data presentation.comprehensive data analysis and GxP solutionsSoftMax Pro Software provides data acquisition, analysis andmanagement capabilities, allowing cross-plate analysis andcustom calculations. For users operating in a FDA 21 CFRPart 11 compliant environment, SoftMax Pro GxP Softwareis available, allowing user permissions, audit trails, e-signatureand reporting tools.Tools for Validation and ComplianceValidation Test Plates for Abs, FI, Lum Optical PerformanceSpectraTest ABS1 Absorbance Plates, FL1 Fluorescence Plates, and LM1Luminescence Plates are used to validate optical performance of SpectraMaxM3, M4, and M5/M5e Microplate Readers.Validation and Compliance of OpticalCharacteristicsSpectraMax Readers have the most complete level ofproduct validation and compliance. Molecular Devicesprovides the complete solution covering the instrumentand software: SpectraTest ABS1, FL1, and LM1 Validation Platesfor hardware validation of absorbance, fluorescence,and luminescence modes IQ/OQ/PQ for all microplate readers SoftMax Pro Software Validation Package Software tools for FDA 21 CFR Part 11 compliance

Automation SolutionsStakMax Microplate Handling SystemCompatible Automation Solutions for SpectraMax ReadersRIon PotentialELISAsQA TestingAnalysisDNA/RNAPurityQuantization &NormalizationSolubility &PermeabilityKinetic AssaysCytotoxicityIntegrate any SpectraMax Multi-Mode Reader with the StakMax MicroplateHandling System from Molecular Devices. The system provides automationfor up to 50 microplates for easy walkaway automation. System setup andcalibration are controlled from within SoftMax Pro Software.SoftMax Pro 5 Software has been integrated by many leading robotics and LIMS providers, enablingboth data analysis and instrument control in automated environments.Robotics Compatibility for increasedthroughputSpectraMax Multi-Mode Microplate Readers canbe easily integrated with our optional StakMax Microplate Handling System for walk-awayprocessing. Operated from within SoftMax ProSoftware, the StakMax System can hold up to 50plates and facilitates barcode reading.For more advanced automation needs MolecularDevices interacts with all of the major labautomation providers, and is one of their leadingchoices.Ordering InformationAcquiring a SpectraMax Multi-Mode MicroplateReader is extremely easy:1. Decide what modes you need and choose thespecific configuration option that suits you best.All systems include SoftMax Pro Software forWindows and Macintosh Operating Systems.2. Choose the additional options you want: Software validation tools SpectraTest ABS1, FL1, LM1 Validation Plates SoftMax Pro GxP Software StakMax Microplate Handing System3. Contact your Molecular Devices salesrepresentative to discuss the details.

SpectraMax multi-mode microplate readersTechnical SpecificationsGeneral SpecificationsDimensions (in.):8.6 (H) x 22.8 (W) x 15.3 (D)Dimensions (cm): 22 (H) x 58 (W) x 39 (D)Weight:36 lbs. (16.4 kg)Power consumption: 420 wattsPower source:100–240 Vac, 3.5 A, 50/60 HzRobotic-compatible: YesGeneral Photometric PerformancePlate formats:6, 12, 24, 48, 96, 384 wellsLight source:Xenon Flash Lamp(1 joule/flash)Detectors:2 photomultiplier tubes (PMT)Shaker time:0 to 999 secondsTemp. control:2 C above ambient to 60 CTemp. uniformity: 1 C at 37 C set pointTemp. accuracy: 1 C at 37 C set pointEndpoint reading : All modesKinetic reading :All modesSpectral scanning: All modesWell scanning:Abs, FI, TRF, LumTypical Read Times (minutes:seconds)*96 wells 384 wellsAbsorbance0:180:49Fluorescence Intensity0:170:48Fluorescence Polarization0:422:03Time-Resolved Fluorescence 0:170:48Luminescence2:007:00* With 3 flashes/well in absorbance and fluorescence modes, and 1 sec./well integration in luminescence.Absorbance Photometric PerformanceReading capabilities:Cuvette or microplateWavelength range:200–1000 nmWavelength selection: Monochromator, tunablein 1.0 nm incrementsWavelength bandwidth: 4.0 nmWavelength accuracy: 2.0 nmWavelength repeatability: 0.2 nmPhotometric range:0–4.0 ODPhotometric resolution: 0.001 ODPhotometric accuracy (microplate): 0.006 OD 1.0%, 0–2 ODPhotometric accuracy (cuvette): 0.005 OD 1.0%, 0–2 ODPhotometric precision: 0.003 OD 1.0%, 0–2 ODStray light: 0.05% @ 230 nmFluorescence Intensity PerformanceReading capabilities: Cuvette or top orbottom of a microplateWavelength range:250–850 nmWavelength selection: Monochromators, tunablein 1.0 nm incrementsBandwidth (EX, EM): 9 nm, 15 nmSensitivity: 5 pM fluorescein in 96 wells orcuvette, 20 pM in 384 wellsLuminescence PerformanceReading capabilities: Cuvette or top or bottomof a microplateWavelength selection: Choice of simultaneousdetection of all wavelengths or selection viamonochromator, tunable in 1.0 nm incrementsWavelength range:250–850 nmSensitivity: 2 fg/well lower detection limit forfirefly luciferase in 96- and 384-well top readDynamic range: 6 decadesCross-talk: 0.3% in white 96- and384-well microplatesTime-Resolved Fluorescence Performance(M4, M5, M5e only)Reading capabilities: Top or bottom of amicroplateWavelength range:250–850 nmWavelength selection: Monochromators, tunablein 1.0 nm incrementsBandwidth (EX, EM): 9 nm, 15 nmPrecision data collection: 1–100 flashes, delayof 0–600 µsec. before read, integration timeselectable between 50–1500 µsec.Sensitivity: 100 fM europium in 96 or 384 wellswith top-readSpectraMax M5e reader only: Certified toCisbio Bioassays’ HTRF assays performancespecificationsFluorescence Polarization Performance(M5/M5e only)Wavelength range:300–750 nmWavelength selection: Monochromators, tunablein 1.0 nm incrementsBandwidth (EX, EM): 9 nm, 15 nmPrecision: 5 mP standard deviation at 1 nMfluorescein in 96 and 384 wellsPatentsThe PathCheck Sensor is covered under U.S.Patents 5,959,738, 6,188,476, 6,320,662,6,339,472, 6,404,501, 6,496,260 and 6,995,844.SpectraMax M3, M4, M5, and M5e readersare also covered under U.S. Patents 6,097,0256,232,608, 6,236,456, 6,313,471 and 6,316,7746,693,709, and 6,825,921.Sales OFFICESUnited States and CanadaMolecular DevicesTel. 1-800-635-5577Fax 1-408-747-3601ChinaMolecular Devices BeijingTel. 86-10-6410-8669Fax 86-10-6410-8601JapanMolecular Devices Japan, OsakaTel. 81-6-6399-8211Fax 81-6-6399-8212United KingdomMolecular Devices (GB) Ltd.Tel. 44-118-944-8000Fax 44-118-944-8001BrazilMolecular Devices BrazilTel. 55-11-3616-6607Fax 55-11-3616-6607Molecular Devices ShanghaiTel. 86-21-3372-1088Fax 86-21-3372-1066Molecular Devices Japan, TokyoTel. 81-3-5282-5261Fax ular Devices GmbHTel. 49-89/96-05-88-0Fax 49-89/9-62-02-34-5South KoreaMolecular Devices Korea, LLCTel. 82-2-3471-9531Fax 82-2-3471-9532The trademarks used herein are the property of 2011 Molecular Devices, Inc. Printed in U.S.A. 6/11 #0120-1498DFOR RESEARCH USE ONLY. NOT FOR USE INDIAGNOSTIC PROCEDURES.Molecular Devices, Inc. or their respective owners.Specifications subject to change without notice.

spectramax multi-mode microplate Readers The SpectraMax M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Choose from a three- (M3), four- (M4), or