Rats! Inquiry-Based Dissection With
Carolina Biological Supply CompanyRats!Inquiry-Based Dissection withCarolina’s Perfect Solution Specimens
Objectives Introduce basic mammalian anatomy using therat Perform a rat dissection focusing on structureand function Perform a dissection using an inquiry-basedapproach Experience the quality of Carolina’s PerfectSolution
The Rat Dissection BioKit (catalog no. 221487)Kit includes: 15 plain Carolina’s PerfectSolution white rats Teacher’s manual withreproducible studentguide
Dissection Preparation Tips Organize your dissection area Apron, gloves, and safety glasses Absorbent pad placed under the dissecting pan(white side facing up) Lay out instruments for easy access
Carolina Workshop MaterialsThese materials are provided to you,compliments of Carolina.Please set up your workstation.
Safety Issues Personal protective equipment– Gloves, goggles, and lab aprons Sharps– Use sparingly; explore with blunt instruments Safety tips– Set down any unused instruments
Prepare to Dissect
Today’s InvestigationWe will explore these sections of the Rat DissectionBioKit : External anatomy Digestive system Urogenital system Circulatory system Respiratory system
Observe External AnatomyList 3 ways the rat isdifferent from humans.List 3 ways the rat issimilar to humans.1.1.2.2.3.3.
Specimen NavigationTransverse planeDorsalFrontal planePosteriorAnteriorVentral
Part I. External AnatomyPre-Dissection Questions1. Where do rats live?2. What do rats eat?3. Do rats bear live young?4. List at least 3 ways in which rats are similar tohumans.5. Describe a rat’s foot structure and for what itmight be useful.
Part I. Procedure1. Lay the specimen on the dissecting tray andobserve the external anatomy of the rat.2. Handle the rat and observe it from all angles.3. Manipulate your specimen’s limbs and take noteof the organism’s articulation.4. Open the mouth and observe the rat’s dentition.
Sexual DimorphismFemale Structures Urethral orifice Vagina AnusMale Structures Urogenital opening Anus Scrotum
Sexual DimorphismUrogenital openingUrethralorificeVaginaAnusScrotumMaleFemale
Part II. Digestive SystemPre-Dissection Questions1. What types of food do rats eat?2. Do rats hunt for their food? If not, how dothey obtain or capture food?1. What sort of food might a rat be better suitedto digest than a human being is?
Part II. Digestive System Begin at the throat. Use scissors. Cut in a posterior direction,to the first opening at thecaudal end of the animal. Lacerate the skin only.
Part II. Digestive SystemUsing scissors andlifting muscle layers asyou cut will preventdamage to underlyingorgans.
Identifying Digestive Organs
Part II. ProcedureDigestive tract: Cut the mesentery so thedigestive tract remains attached. Trace thepath of food. Identify structures and recordobservations.
Part III. Urogenital System
Part III. Urogenital System Kidneys Hilus Adrenal glands (part of the endocrine system) Renal artery Renal vein Ureter Bladder Urethra Male Penis* Urogenital duct
Pregnant Female Rat
Pregnant Female Rat
Pregnant Female RatHow does thereproductivesystem of a femalerat allow formultiple developingfetuses?
Investigating Internal AnatomyDevelop a question about internalanatomy to investigate.Why are rats used so frequently as laboratoryspecimens?How does the digestive system of a rat differ fromhumans?What portion of the rat brain seems mostpronounced? Why is this advantageous for thesurvival of rats?
Guided InquiryBenchmarks to guide student dissectionList major organs students should locate and identify. Esophagus Ovaries Stomach Testes Liver Lungs Small intestine Heart Kidney Spleen Urinary bladder Major muscle groups
Additional ActivitiesHave students create a flowchart oforgans for each all intestinepancreascaecumlarge intestinerectumanus
Additional Activities Determine points where systems interconnect. Identify purpose for interconnection.Respiratory/Circulatory Systems connect wherecapillaries that branch from thepulmonary arteries surround thealveolus of the lungs; gasexchange takes place to supplyO2 to blood cells and take awayCO2.
Internal Anatomy terus
eGenus:HomoSpecies:Homo sapiens
Organ System Jigsaw1. Assign each group oneof the major organsystems:a. Circulatory andRespiratoryb. Digestivec. Urogenitald. Muscular andNervous
Organ System Jigsaw2. Complete the dissectionof the selected organsystem according to theguide.3. Make note of any tips fordissection to share withthe group at the end ofthe session.
We Can Meet Your Dissection NeedsTop-quality specimensand supplies
Additional Resources from CarolinaCarolina BioLab Virtual Lab SeriesGuide studentsthrough aninteractive virtualdissection, teachinginternal andexternal features.
Carolina Free ResourcesCarolina offers many freeresources to help supportteachers.
Time to Clean Up . . .Carolina’s Perfect Solution specimens Return to white bucket or take home forfurther study All other waste in the trash bagsDissecting pans and instruments Clean or take as giftSafety glasses and aprons Gift from CarolinaEvaluation forms/info cards Complete, return to presenter
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Carolina Biological Supply CompanyThank you for investing your time inour training program.For all of your classroom needs, checkout our Web site, www.carolina.com.Enjoy the rest of the conference!
Introduce basic mammalian anatomy using the rat Perform a rat dissection focusing on structure and function Perform a dissection using an inquiry-based approach Experience the quality of Carolina’s Perfect Solution Objectives
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Les rats ont été répartis en fonction de leurs traitements en 3 lots de 8 rats chacun : - Un lot de rats témoins (T). - Un lot de rats traités quotidiennement par le TEB à une dose de 100 mg/kg par voie intrapéritonéale. - Un lot de rats recevant une reprise journalière de TEB avec une alimentation enrichie avec le
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