Olympus BHS (BH-2) System Microscope Instructions
MODELBHSSYSTEM MICROSCOPEThis instruction manual is for use of your instrument.Before putting it into operation, we recommend youread this manual carefully in order to familiarize yourself fully with its use so that you may obtain optimum performance.
This instruction manual has been written for the use of the Olympus System Microscope ModelBHS. It is recommended that you read the manual carefully in order to familiarize yourself fullywith the use of the microscope, so that you can obtain optimum performance from it.IMPORTANTObSe ethe following points:Operation1.Always handle the microscope with the care i t deserves, and avoid abrupt motions.2. Avoid the use of the microscope in direct sunlight, high temperature and humidity, dustand vibration.3.Only use the tension adjustment ring for altering the tension of the coarse adjustmentknobs. (Do not twist the two coarse adjustment knobs in opposite directions simultaneously, as this will cause damage.)4.Make it a point of grounding the microscope to prevent electric accidents.rn Maintenance1.Lenses must always be kept clean. Carefully wipe off oil or fingerprints deposited on thelens surfaces with gauze moistened with a small amount of xylene, alcohol or ether.2.Do not use organic solutions to wipe the surfaces of various components. Plastic parts,especially, should be cleaned with neutral detergent.3.Never disassemble the microscope for repair. Only authorized Olympus service personnelshould make repairs.4. The microscope should be covered with the vinyl dust cover provided and stored in a placefree from humidity.
CONTENTS.2.3.4IV.IDENTIFICATION AND FUNCTION OF VARIOUS COMPONENTS . . . . . . . . . .6V.OPERATION.91.STANDARD EQUIPMENTII.NOMENCLATUREIll.ASSEMBLYA. Switching on the Light Source/voltage Adjustment and Light IntensityB.C.IPlacement of a Specimen Slidepzzzizq . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ./specimen slide 1Observation Tube.10111. lnterpupillary Distance Adjustment2. Diopter Adjustment3. Light Path Selector0 . Condenser Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1. Condenser Centration12l i e l dlris i a h r a g m ll p e r t u r elris Dia hranm1E.Focusing Adjustment.131. Tension of Coarse Adjustment Knobs and Fine Adjustment/use of Rubber C forD Fine Adiustment n o b l2. Pre-Focusing Lever3. Adjustment of Stage Block Height.14.16F.Use of Immersion ObjectivesG.PhotomicrographyVI.OPTICAL DATAVII.TROUBLESHOOTING GUIDE.17
I.STANDARD EQUIPMENTModelComponentBHS-112 BHS-113 BHS-312 BHS-313Microscope standBinoculartubeBHS-F1111BH2-B 130110000110bservation SC11110101Square mechanical stage withright-hand low drive tic/AplanaticBH2-AACcondenserHalogen lamp housingBHS-LSH1111Halogen bulbs12V100WHAL-L2222D Plan 4X,D Plan 1OX,D Plan 20X,D Plan 40X.D Plan 1OOX (oil)leach01 each0SSSSS01 each01eachObjectivesPlan 4X.Plan 10X.Plan 20X,Plan 40X,Plan 100X (oil)EyepiecesWH K1OX2222Photo eyepieceNF K3.3X0011Line cordUYCP1111FilterKB-41111Immersion oil, bottled1111Vinyl dust cover1111Allen wrench1111.
II.NOMENCLATUREThe Model BHS consists of various components and interchangeable accessories as shown in thephoto below. A wide variety of combinations, standard or optional, is available according to yourrequirements.Observation tube7Microscope standFor biological use.Halogen lamphousina
Ill.ASSEMBLYThis picture illustrates the sequential procedure of assembly. The numbers indicate theorder of assembly of various components. Remove dust caps before mounting components.Take care to keep all glass surfaces clean, and avoid scratching the glass surfaces.NOTE: For numbers 0 ,0 ,0 and 0 please refer to explanations in detail on the nextpage.Circular dovetail mountHalogen bulb(The N.A. scaleengraved on the condenser should face themicroscope front.)Halogen lampMicroscope standLine cord@Outlet
Explanations in detailQ Mounting the halogen bulb1) Releasing the bulb clamping leve @ ofthe collector lens @ in the dir tion ofof thethe arrow, insert two contacthalogen bulb into the socket Q. (Fig. 1)(Recommended to use a glove or gauze tohandle the halogen bulb.)2) Secure the bulb in position with the twolevers.?.E* Before use, wipe off any fingerprints orstains on the bulb.Fig. 10 Mounting the stage1) Loosen the stage clamping screw @ byrotating counterclockwise. (Fig. 2)2) Insert the stage into the mounting dovetail of the microscope stand slowly andlock with clamping screw.Fig. 20 Mounting the revolving nosepiece1) Loosen the nosepiece clamping screw @.(Fig. 3)2) Aligning the nosepiece dovetail sl detothe mounting block @, push in the nosepiece slowly all the way.Fig. 3@ Mounting the observation tubeW'-*"1) Loosen the clamping knob @ fully. Pullspring-loaded clamping knob @ . Thiswill cause the locating pin @ to withdraw.(Fig. 4) If the pin does not, loosen thescrew further until the pin withdraws.2) With clamping knob @ pulled out, insertthe circular dovetail of the observationtube into the ring dovetail.3) Tighten the clamping knob.Fig. 4
IV.IDENTIFICATION AND FUNCTION OF VARIOUS COMPONENTSLight path selector knob/The knob can be operatedin 3 positions to deflect thelight as desired.Tension adjustment ringClockwise rotation increasescoarse adjustment tension.adjustment knobCoarse adjustmentrange: 2 6 m mX-axis low drivecontrol knobAccepts the NDfilter holder.X excursion range:7 6 mm.Filter diameter 45mm.Sliding voltage control leverVoltage lowers as the lever ispulled toward the microscopefront. D o not disengage the ND filter for about a few minutes after the use, in order to give time tocool. Other filters (e.g. KB-4 provided, etc.) should be inserted into the filter mount at the lightexit on the microscope base.
Photo tube-.Diopter adjustmentringCondenser heightad iustment knobArrow mark Q 0 indicatesincrease in diaphragm diameter.*This breaker protrudes i t s central part t o cut off the electric power in case of the dimmercircuit trouble (due to short circuit, etc.) or over current. To restore the breaker, press thecentral part. If the breaker is actuated again, disconnect the line cord from the AC outlet andcontact the Olympus Service Center.
Summary of Putting the Microscope into OperationModel BHSA.Switch on the light source (page 9).8.Place a specimen slide on the mechanical stage (page 9).C.Coarse focus with the 10X objective.D.Make interpupillary distance and diopter adjustments (page 11).E.Adjust the condenser position (page 12).F.Swing in the desired objective.G.Adjust light intensity.H.Fine focus.I.Adjust aperture iris diaphragm and field iris diaphragm (page wing-outcondenserLow powercondenserSwing out toplens*Compatibles w n g ntopII, ? .,,:;;?.):.: t.-,,*To obtain better illumination for photomicrography with a 2X objective, it is recommended to use the ultra-low condenser BH2-ULC, optionally available.**N.A. is somewhat low, but still compatible with a 100X objective.(Cut off this page at dotted line and put it on the wall near the microscope for use as a reminder of microscopic procedure.)
OPERATIONV.A.Switching on the Light Source1) Place the sliding voltage control lever on theright side of the microscope base to a position closest to you (low voltage position).Switch on the light source. (Fig. 5)Fig. 5[ Voltage Adjustment andLight IntensityIAs you push the control levera in the direction of the arrow in order to obtain increasing intensity (Fig. 6), the LED readout @will display the lamp voltage.* TwoLEDs on the left side indicate thevoltage from OV to 6V, and twelve LEOSonthe right side from 6.5V t o 12V in 0.5Vincrements. The indication with the letters"PHOTO" can be used as a guide line forcolor photomicrography.B.Fig. 6Placement of a Specimen Slide1) Rotate the coarse adjustment knobs @ inthe direction of the arrow to rack down thestage so that a specimen slide can be placedon the stage. (Fig. 7)NOTE: The rotation of the coarse and fineadjustment knobs in the direction ofthe arrow will rack down the stage.Fig. 72) Opening the spring-loaded finger of thespecimen holder with one hand, place aspecimen slide inside the holder. (Fig. 8)When the slide comes in contact with theback of the specimen holder, slowly returnthe spring-loaded finger.WARNING: I f the spring-loaded finger isreturned quickly, it may causedamage t o the specimen slide.Fig. 8
I Cover Glass IA n Olympus objective engraved "16010.17" requires a cover glass o f 0.17mm thickness.I f the numerical aperture o f the objective is 0.7 or higher (except immersion objectives) andno correction collar is provided, the resolving power deteriorates t o a great extent if coverglass thickness deviates from the above listed value.NOTE: I n some countries a 0.17mm cover glass corresponds t o a designation o f # I % .A cover glass (0.4mm thick) for blood counting, etc. can be used with Olympus objectivesof the working distance longer than 0.4mm. (See VI. OPTICAL D A T A at page 16.)1 Specimen Slide ISpecimen slides 0.8 m m t o 1.2 m m thick arerecommended for Olympus objectives.3) Bring the portion o f the specimen for observation into the light path by means of thelow drive control knobs. (Fig. 9)* Tighten the stage clamping screw @ in themicroscope front.The specimen holder can accommodate twostandard specimen slides simultaneously.The specimen holder is removable t o obtaina large unobstructed stage surface to holdspecimens up t o 5 5 mm x 8 5 mm.Fig. 9T o rotate the stage loosen the stage clampingscrew @ and holding this screw, rotate thestage into the desired direciton. (Fig. 9 )Q Stage clips@, for use with immersion objectives. (Fig. 10)A pair of stage clips are optionally availablet o hold the specimen on the stage, eliminating a specimen drag caused by immersion oilbetween slide and stage surface. The clipscan be inserted into the holes provided o nthe specimen holder.Fig. 10
C.- ,-.-.Observation Tube-1. lnterpupillary Distance Adjustment1) Click the 10X objective into position.2) Looking through the eyepieces with botheyes, adjust the interpupillary distance ofthe binocular tube by adjusting theknurled dovetail slides @ of the right andleft eyepiece tubes with both hands untilperfect binocular vision is obtained. (Fig.11)Fig. 11- ----v---z*!- F-2. Diopter Adjustment1) Look at the image through the right eyepiece with your right eye and focus onthe specimen with the fine adjustmentknobs.2) Next, look at the image through the lefteyepiece with your left eye and rotate thediopter adjustment ring @ t o focus onthe specimen without using the coarseand fine adjustment knobs. (Fig. 12)Fig. 123. Light Path Selection1) The trinocular tube is provided with alight path selector knob @ to direct thelight to the observation tube and/or tothe photo tube in 3 positions (Fig. 13)-. - r "Fig. 13Indicator PlateIAmountof lightApplicationIKnob PositionI/IPushed in all the way(V)IPulled out halfway(C. V)Pulled out all the way(C)20% into binocular tube100% into binocular tube 80% into photo tube1100% into photo tube@ Observation@ Dark specimens@ Observation of exces-Photomicrography ofsively bright specimens dark specimens@ Photomicrography (focusing through the binocular tube)The indicator plate is provided at the knob port to summarize the usage of the abovetable; i t can be consulted before operating the knob.V: Viewer (white letter)C .V: C,amera & viewer (yellow-green letters)C: Camera (red letter)The colors of the letters correspond with the color bands on the knob shaft.
D. Condenser Adjustment1. Condenser Centration1) Stop down the field iris diaphragm withby rotating in the direcknurled ringtion of the arrow. (Fig. 14)2) Use the condenser height adjustmentknob @ t o move the condenser up anddown until an image of the field diaphragm can be seen clearly in the eyepieces. The rotation of the knob in thedirection of the arrow lowers the condenser.Fig. 15Fig. 14\3) Bring the field iris diaphragm image into the center of the field of view with the two condenser centering knobs @. (Fig. 14)4) Widen the diameter of the iris diaphragm progressively. I f the polygonal image of the irisdiaphragm becomes inscribed in the field i t means that the field diaphragm is centered.(Fig. 15)* Ifthe specimen slide used is thicker than 1.2mm, you cannot obtain the image of the field iris diaphragm. Especially for photomicrography, use a specimen slide 0 . 9 m m to 1.2mm as much as possible.1Field lris Diaphragm]The field iris diaphragm controls the diameter of the ray bundle impinging on the specimen surface and therefore, by stopping down the field diaphragm until it is slightlylarger than the field o f view, it can reduce stray light, which in turn increases image definition and contrast.Aperture lris DiaphragmIn order to achieve optimum objective performance, the opening of the aperture irisdiaphragm should be matched to the numerical aperture of the objective in use. I t isoften preferable, however, t o stop down the aperture diaphragm slightly more than indicated by the objective N.A. This will result in better image contrast, increased depth offocus and a flatter field.IAfter completing focus adjustment, remove one of the eyepieces from the observation tube and look into the emptyeyepiece tube. As you stop down the aperture iris diaphragm, the image of the irisdiaphragm can be seen in the objectivepupil. Adjust the opening of the diaphragm to match the N.A. of the objectivein use. If the specimen is low in contrast,it is recommended to stop down to 70%t o 80% of the objective N.A. (Fig. 16)Fig. 16
E.Focus Adjustment1. Tension of Coarse Adjustment Knobs andFine Adjustment.Although the tension of the coarse adjustment knobs has been already adjusted foroptimum performance by the manufacturer,it is possible to personally adjust the tensionof the coarse adjustment for either heavy orlight movement depending on the operator'spreference b y rotating the tension adjustFig. 17ment ring @. (Fig. 17)The ring can be rotated by inserting a screwdriver into one of the holes on the periphery ofthe ring. The clockwise rotation (in the direction of the arrow) tightens the coarse adjustment knobs. Do not loosen the ring too much, because the stage may drop or the fineadjustment knobs may slip.NOTE: Do not rotate the right and left coarse adjustment knobs in the opposite directionssimultaneously. If the stage drops and the specimen goes out of focus, the tensionadjustment ring is too loose. Tighten the ring.[Use of Rubber Cap for Fine Adjustment KnobtIAttaching this cap over the fine adjustmentknob increases the sensitivity of the fine focusing motion. (The rubber cap i s optionallyavailable.)2. Pre-Focusing LeverThis lever @ is provided to prevent possiblecontact between specimen and objective aswell as to simplify coarse focusing. (Fig. 18)The lever is locked after coarse focus hasbeen accomplished. This prevents furtherupward travel of the stage by means of thecoarse adjustment knobs, and automaticallyprovides a limiting stop if thestage is loweredand then raised again. The pre-focusing leverdoes not restrict fine focusing.Fig. 183. Adjustment of Stage Block HeightIn addition t o the vertical movement of thestage by means of coarse and fine adjustments, the stage block height can be changedfor observation of specimens which arethicker than standard slides, e.g. chambers,flasks, etc. with much larger thickness.The stage block height can be adjusted byloosening the stage block locking screw @with the Allen wrench provided and retightening it at the lower position. (Fig. 19)Fig. 19
F.Use of Immersion Objectives1) Focus the specimen with a low power objective.2) Put a drop of immersion oil on the specimen slide and the front lens of the immersionobjective.3) Turn the revolving nosepiece to bring the immersion objective into the light path, and focuswith the fine adjustment knobs.NOTE:@ Forimmersion condensers such as an achromatic-aplanatic condenser or Abbecondenser, remove the specimen from the mechanical stage and place a drop ofimmersion oil on the front lens of the condenser. Then, place the specimen onthe stage and slowly raise the condenser until firm contact with the underside ofthe specimen slide is made.@ Care shouldbe taken t o prevent oil bubbles from forming in the oil film betweencondenser and specimen slide. If any, re-apply immersion oil, for these bubblesgreatly deteriorate the lens performance.@ Afteruse carefully wipe off the immersion oil deposited on the lens surfaceswith gauze moistened with xylene. Never leave oil on the lens surfaces after useas oil remnants will seriously impair the performance of the lens system.G. PhotomicrographyThe Olympus Photomicrographic Equipment Model PM-IOAD is uniquely qualified to beused with the BHS microscope for routine and advanced photomicrography. A separate,detailed instruction manual is available for the PM-IOAD camera system.For quick reference, however, you may want to refer to the follow ngpointers when usingthe PM-IOAD.1. Photographic EyepieceUse NFK photo eyepieces for photomicrography.Insert the eyepiece @) into the eyepiece tubeof the photo tube. (Fig. 20)Fig. 202. Mounting the Photographic UnitSlip the body of the photographic unit over thephoto tube. Align the dots on photo tube andthe PM-1OAD body and clamp the camera unitto the photo tube. (Fig. 21)3. Setting the Light Path SelectorRefer to section C.3. on page 11.Fig. 21
4. Color and B & W Photomicrographya) Color photomicrographyVoltage setting . . . . . . . . Within a range as marked with the letters "PHOTO" onthe voltmeter.Filter . . . . . . . . . . . . . . . Insert the LBD-2 filter into the filter mount at the lightexit on the microscope base. (in case of daylight film)Light intensity . . . . . . . . Should be adjusted by means of the ND filters ratherthan by means of voltage change.The range of voltage setting mentioned above indicates a guide line for color photomicrography. I f the photograph, taken this way, however, gives bluish tint, lower thevoltage slightly, and i f i t gives reddish tint, raise the voltage slightly.b) Use of contrast filters for B & W photomicrography,@ Green filter . . . . . This filter is most recommended, since it aids i n obtaining photomicrographs of highest resolution and maximum contrast.@ Yellow filter . . . . Recommended as contrast filter when i t is desirable t o emphasizethe blue color in the stained portion of a specimen.@ Cobalt filter . . . . Not generally recommended for photomicrographic purposessince it reduces both resolution and contrast. Use it for specificapplications only when it is desirable to emphasize yellow in thestained portion of a specimen.@ No filter . . . . . . . A photograph is taken with less contrast than with thegreen filter.5. Focusing ProcedureUse the field of view eyepieces for focusing on the film plane. Each field of view eyepiecehas a focusing front lens and a reticle with 4 frames, each frame indicating the area coveredby a specific power NF K photo eyepeice. (Fig. 22).The number at each frame indicates themagnification of the photo eyepiece. Thec,s71image in the field of view eyepiece and theimage on the film plane are in focus at theL&,J J same time. Several type field of view eyepieces are available, according to the, filmsize employed.Fig. 22 :Field
This instruction manual has been written for the use of the Olympus System Microscope Model BHS. It is recommended that you read the manual carefully in order to familiarize yourself fully . Specimen slides 0.8 mm to 1.2 mm thick are recommended for Olympus objectives. 3) Bring the portion of the specimen for obser- vation into the light path .
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