Step 1: Transcription Step 2: Translation

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Step 1: TranscriptionStep 2: TranslationWHAT is it?WHEREdoes ithappen?WHAT is it?WHEREdoes ithappen?WHY is thisstepnecessary?DNA holds the code for a living organisminside the . But proteins are made at. mRNA acts like theand takes the code from theto the in the cytoplasmwhere the protein can be made.HOW doesit occur?STEPS:1. UNZIP: An unzips the DNA2. MAKE mRNA:Use the DNA template to make(messenger RNA)REMEMBER!: RNA uses instead of3. LEAVE: mRNA leaves the and goesinto the to find a ribsome.You try it!DNA mRNATranscribe the DNA!DNA: A T C C G A G T TmRNA:WHY is thisstepnecessary?HOW doesit occur?Messenger RNA ( ) carries the genetic codefrom the DNA in the to the ribosome in the. Translation converts themessage into the !STEPS:1. FIND RIBOSOME: mRNA attaches to a2. READ: Ribosome “ ” the mRNA in groups ofbases (a CODON)3. tRNA MATCHES: A molecule comes alongwith the right to match the codonCODON UAGANTICODON 4. LINK AMINO ACIDS:the links the aminoacids together with bonds to make a(protein)!You try it!mRNA codons anticodonTranslate the mRNA!mRNA:U A GtRNA anticodon:G C UC A A

What is RNA?RNA: Ribonucleic eoxyribose sugarBases:You try it!1. Name atheCodonamino acidsChartthat are coded by theUsingfollowing codons:a. AAA d. CAC b. UAC e. UGA c. GGG f. AGC 2. Write the amino acid sequence to make up theprotein: GCA – GGU – CCA – AUG – UGCBases:What can we conclude? codon will make amino acid But! some amino acids can be made withmultiple codons! This means we can make the same proteinseven if the codons get changed a little, akalife’s insurance policy!3. Write the amino acid sequence to make up theprotein: GCA – GGU – CCG – AUA – UGC

Practice Protein Synthesis.Now you try. Fill out the template strand on the5. Is the following strand a segment of DNA or RNA? Explain how you know!A G C U A C C G U A C G U A6. Is the following strand a segment of DNA or RNA? Explain how you know!A C G T A C G T A G C T T A7. Fill in the blanks of the protein synthesis summary below:DNA mRNA . Takes place in the .mRNA PROTEIN . Takes place in the .tRNA carries the (2 words) that will bond together to form a polypeptide (protein).8. Is mRNA synthesized in translation or transcription?9. mRNA has codons or anti-codons?10. 1 or 3 codons equal one amino acid?11. tRNA brings amino acids to the nucleus or ribosome?12. A polypeptide is a sequence of proteins or amino aicds?13. tRNA transfers amino acids during translation or transcription?Use the following DNA sequence for questions 8-10.DNA TAC CAT CCG GAA TCC CCT ATT1. After transcription the result would be:2. After translation the result would be:3. Protein synthesis produces proteins for the cell. What are some examples of proteins that we have discussedin this class already? Name at least 2. &

Practice!4. Amino acids link together by peptide bonds to form proteins. The cellular organelle performing this is theA. MitochondriaB. ChloroplastC. RibosomeD. Nucleus5. Which of these is most responsible for carrying coded information out of the nucleus into the cytoplasm?A. ATPB. mRNAC. DNAD. tRNA6. The process of DNA replication is necessary before a cellA. makes a proteinB. codes for RNA moleculesC. divides into two cellsD. modifies lysosome enzymes7. Genetic information usually flows in one specific direction (aka The Central Dogma of Biology). Which of thefollowing best represents this flow?A. DNA Protein RNAB. Protein RNA DNAC. RNA Protein DNAD. DNA RNA Protein8. What process does this diagram represent?A.B.C.D.DNA replicationTranscriptionTranslationMutationUSE THE WORDS TO LABEL THEPICTURE to the ONNucleusCytoplasmProtein (Polypeptide)Amino acidCodonAnticodon

8. Is mRNA synthesized in translation or transcription? 9. mRNA has codons or anti-codons? 10. 1 or 3 codons equal one amino acid? 11. tRNA brings amino acids to the nucleus or ribosome? 12. A polypeptide is a sequence of proteins or amino aicds? 13. tRNA transfers amino acids during

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