SEQUENTIAL CULTURE MEDIA SYSTEM - Cook Medical
SEQUENTIAL CULTURE MEDIA SYSTEM
ContentsGamete solutions. 7Fertilization solutions.15Cleavage solutions.21Blastocyst solutions.25Complete list of order numbers.30
Nurture gametes and embryoswith the nutrients they need.Our culture system mimics the delicateand dynamic environment of the femalereproductive tract by providing nutrients tomeet the shifting metabolic requirementsof gametes and embryos.
GAMETESOLUTIONSGamete collection and preparationmight be the most stressful processin IVF, and the environmentaldisruption that occurs can significantlyimpair gamete viability. These gametesolutions meet the metabolicrequirements of the oocyte andhelp to maintain oocyte homeostasisso that subsequent steps in IVFcan succeed.7
IONSBLASTOCYSTSOLUTIONS8
SYDNEY IVF GAMETE BUFFERSydney IVF Gamete Buffer is used to physically wash andstore gametes in preparation for the fertilization step in theIVF process. HEPES buffered Designed to prepare oocytes and maintain a stable environment during washing of cumulus-enclosed oocytes. Designed to prepare sperm for swim-up, densitygradient, and diluent procedures Can be used during ICSICalcium lactateD-glucoseGentamicinGlutamine, stabilizedGlycineHEPESHuman serum albumin (HSA)*L-alanineL-asparagineL-aspartic acidL-glutamic acidL-prolineL-serineMagnesium sulphatePotassium chloridePotassium phosphatePurified waterSodium bicarbonateSodium chlorideSodium pyruvateTaurinepH (in air): 7.3-7.5Osmolality: 285-295 mOsm/kg2-cell MEA: 80% of control expandedto blastocysts at 72 hoursEndotoxins: 0.4 EU/mLShelf life: 20 weeks from date of manufactureSterile: Aseptically filteredHSA assay: 5.00–12.50 mg/mLHSSA: 90% of control at24 hoursOrderNumberG46320G46318G46317ReferencePart L2050100Sydney IVF Follicle Flush BufferSydney IVF Spermient Sydney IVF Sperm Gradient KitCook Flexipet PipettesBLASTOCYSTSOLUTIONSRELATED PRODUCTSCLEAVAGESOLUTIONSRELEASE SPECIFICATIONSSydney IVF Gamete Buffer is designed for gamete washing,swim-up procedures, and use as a diluent in densitygradient and sperm-thawing procedures. It can also be usedfor ICSI when a HEPES-buffered environment is required.After ovum aspiration, Sydney IVF Gamete Buffer is used towash the cumulus-oocyte complex before it is transferredinto Sydney IVF Fertilization Medium. The nonessentialamino acids prevent the depletion of the oocyte’s internalpool of amino signed to be used in atmospheric air conditions, notan enriched CO2 environmentCONSTITUENTSWe strongly recommend that you record the patient name and lot number every time you use Cook Medical IVF media in a clinical procedure.*We take standard measures to prevent our medicinal human-blood and plasma products from causing infections. These steps include screening individual donationsand plasma pools for specific markers of infection and inactivating and removing viruses during manufacture. Despite these safeguards, a possibility exists that medicinalblood or plasma products will transmit infective agents, including unknown or emerging agents or other pathogens. No reports of proven virus transmission have beenlinked to albumin that was manufactured by established processes to European Pharmacopoeia specifications. We strongly recommend that you record the patient nameand lot number every time you use Cook Medical IVF media in a clinical procedure.9
SYDNEY IVF SPERM MEDIUMSydney IVF Sperm Medium is intended for use during invitro fertilization procedures to process sperm.GAMETESOLUTIONS Used for sperm preparation and storage Recommended for swim-up and sperm washing in6% CO2USAGEFERTILIZATIONSOLUTIONSSydney IVF Sperm Medium is designed for use with theswim-up technique to separate motile populations of sperm.Intrauterine insemination can be performed in this medium,or sperm can be resuspended in Sydney IVF FertilizationMedium prior to the insemination of oocytes in vitro. Theproduct should be equilibrated in 6% CO2 before use.OrderNumberG46316G46315G46314ReferencePart L2050100CONSTITUENTSCalcium lactateD-glucoseGentamicinGlutamine, stabilizedGlycineHuman serum albumin (HSA)*Magnesium sulphatePotassium chloridePotassium phosphatePurified waterSodium bicarbonateSodium chlorideSodium pyruvateTaurineRELEASE SPECIFICATIONSpH (in air): 7.5-7.8Osmolality: 285-295 mOsm/kg2-cell MEA: 80% of control expandedto blastocysts at 72 hoursEndotoxins: 0.4 EU/mLShelf life: 20 weeks from date of manufactureSterile: Aseptically filteredHSA assay: 5.00–12.50 mg/mLRELATED PRODUCTSCLEAVAGESOLUTIONSSydney IVF Gamete BufferSydney IVF Sperm Gradient KitSydney IVF Fertilization MediumIUI cathetersBLASTOCYSTSOLUTIONSWe strongly recommend that you record the patient name and lot number every time you use Cook Medical IVF media in a clinical procedure.*We take standard measures to prevent our medicinal human-blood and plasma products from causing infections. These steps include screening individual donationsand plasma pools for specific markers of infection and inactivating and removing viruses during manufacture. Despite these safeguards, a possibility exists that medicinalblood or plasma products will transmit infective agents, including unknown or emerging agents or other pathogens. No reports of proven virus transmission have beenlinked to albumin that was manufactured by established processes to European Pharmacopoeia specifications. We strongly recommend that you record the patient nameand lot number every time you use Cook Medical IVF media in a clinical procedure.10
SYDNEY IVF SPERM GRADIENT KITSydney IVF Sperm Gradient is intended for use during invitro fertilization procedures to separate motile sperm fromseminal plasma.Sold as a convenient two-part kit for separating motilesperm Contains silane-coated silica Includes one vial of 40% solution and one vial of 80%solutionUSAGEReferencePart NumberK-SISG-20-AAK-SISG-50-AARELEASE SPECIFICATIONSpH (in air): 7.3-7.5Osmolality: 285-295 mOsm/kg2-cell MEA: 80% of control expandedto blastocysts at 72 hoursEndotoxins: 0.4 EU/mLShelf life: 20 weeks from date of manufactureSterile: Aseptically filteredHSA assay: 5.00–12.50 mg/mLVolumemL20 (x2)50 (x2)RELATED PRODUCTSSydney IVF Gamete BufferSydney IVF Sperm MediumSydney IVF Fertilization ssium phosphatePurified waterSilane-coated silicaparticlesSodium bicarbonateSodium chlorideSodium pyruvateTaurineFERTILIZATIONSOLUTIONSSydney IVF Sperm Gradient was designed for use in spermpreparation via density-gradient separation. The gradientsare prepared by adding 1.5 mL of 40% solution underlaidwith 1.5 mL of 80% solution in a conical-bottom test tube.The liquefied semen is then overlaid onto the gradients. Thetest tube is centrifuged, and the resultant pellet is aspiratedand washed in Sydney IVF Gamete Buffer. The final pellet isresuspended in either Sydney IVF Sperm Medium or SydneyIVF Fertilization Medium.Calcium lactateD-glucoseGentamicinGlutamine, stabilizedGlycineHEPESHuman serum albumin (HSA)*Magnesium sulphatePotassium chlorideGAMETESOLUTIONS CONSTITUENTSBLASTOCYSTSOLUTIONSWe strongly recommend that you record the patient name and lot number every time you use Cook Medical IVF media in a clinical procedure.*We take standard measures to prevent our medicinal human-blood and plasma products from causing infections. These steps include screening individual donationsand plasma pools for specific markers of infection and inactivating and removing viruses during manufacture. Despite these safeguards, a possibility exists that medicinalblood or plasma products will transmit infective agents, including unknown or emerging agents or other pathogens. No reports of proven virus transmission have beenlinked to albumin that was manufactured by established processes to European Pharmacopoeia specifications. We strongly recommend that you record the patient nameand lot number every time you use Cook Medical IVF media in a clinical procedure.11
SYDNEY IVF SPERMIENT Sydney IVF Spermient is intended for use during in vitrofertilization procedures to separate motile sperm fromseminal plasma.GAMETESOLUTIONS Sold at a 100% concentration of silane-coated-silica–based stock solution to enable customization of spermdensity separation Can be diluted with Sydney IVF Gamete Buffer to anyrequired concentrationUSAGEFERTILIZATIONSOLUTIONSSydney IVF Spermient is a 100% stock solution that shouldbe diluted with Sydney IVF Gamete Buffer to the densityrequired for use (for example, 80% and 40%).* A one- ortwo-layer gradient is then prepared in a conical-bottomtest tube. The liquefied semen is overlaid on the gradient.The tube is centrifuged, and the resultant pellet is aspiratedand washed in Sydney IVF Gamete Buffer. The final pellet isresuspended in either Sydney IVF Sperm Medium or SydneyIVF Fertilization erencePart TUENTSCalcium lactateD-glucoseGentamicinGlutamine, stabilizedGlycineHEPESHuman serum albumin(HSA)**Magnesium sulphatePotassium chloridePotassium phosphatePurified waterSilane-coated silicaparticlesSodium bicarbonateSodium chlorideSodium pyruvateTaurineRELEASE SPECIFICATIONSpH (in air): 7.3-7.5Osmolality: 285-295 mOsm/kg2-cell MEA: 80% of control expandedto blastocysts at 72 hoursEndotoxins: 0.4 EU/mLShelf life: 20 weeks from date of manufactureSterile: Aseptically filteredHSA assay: 5.00–12.50 mg/mLRELATED PRODUCTSSydney IVF Gamete BufferSydney IVF Sperm MediumSydney IVF Fertilization MediumBLASTOCYSTSOLUTIONSWe strongly recommend that you record the patient name and lot number every time you use Cook Medical IVF media in a clinical procedure.*Use of this medium at dilutions below 40% and above 80% has not been evaluated and therefore is not recommended.**We take standard measures to prevent our medicinal human-blood and plasma products from causing infections. These steps include screening individual donationsand plasma pools for specific markers of infection and inactivating and removing viruses during manufacture. Despite these safeguards, a possibility exists that medicinalblood or plasma products will transmit infective agents, including unknown or emerging agents or other pathogens. No reports of proven virus transmission have beenlinked to albumin that was manufactured by established processes to European Pharmacopoeia specifications. We strongly recommend that you record the patient nameand lot number every time you use Cook Medical IVF media in a clinical procedure.12
SYDNEY IVF FOLLICLE FLUSH BUFFERSydney IVF Follicle Flush Buffer is intended for use during invitro fertilization procedures for follicle flushing and oocytecollection.HEPES buffered Suitable for flushing needles and lines Contains nonessential amino acids Protein freeUSAGEOrderNumberG46308ReferencePart NumberK-SIFB-100-AARELATED PRODUCTSRELEASE SPECIFICATIONSpH (in air): 7.3-7.5Osmolality: 285-295 mOsm/kg2-cell MEA: 80% of control expandedto blastocysts at 72 hoursEndotoxins: 0.4 EU/mLShelf life: 20 weeks from date of manufactureSterile: Aseptically filteredCLEAVAGESOLUTIONSSydney IVF Gamete BufferAspiration needlesCook Vacuum PumpTest Tube HeaterVolumemL100L-prolineL-serineMagnesium sulphatePotassium chloridePotassium phosphatePurified waterSodium bicarbonateSodium chlorideSodium pyruvateTaurineFERTILIZATIONSOLUTIONSSydney IVF Follicle Flush Buffer contains nonessential aminoacids. This solution is HEPES buffered so that it maintains pHat 37 C without the use of a CO2 incubator.Calcium lactateD-glucoseGentamicinGlutamine, tic acidL-glutamic acidGAMETESOLUTIONS CONSTITUENTSBLASTOCYSTSOLUTIONSWe strongly recommend that you record the patient name and lot number every time you use Cook Medical IVF media in a clinical procedure.13
FERTILIZATIONSOLUTIONSAchieving a high fertilization rateincreases the potential success ofany treatment cycle, and successfulfertilization—whether ICSI orinsemination—depends in part onthe fertilization media. Theseformulations are made with carefullyselected ingredients and stringentquality-control procedures.15
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SYDNEY IVF CULTURE OILSydney IVF Culture Oil is intended to be used as an oiloverlay for culture of gametes, zygotes, or embryosin assisted reproductive technology (ART) andmicromanipulation procedures.Specifically designed for human IVF Extensively tested to ensure quality– Tested for peroxide value– Tested for peroxide toxicity with a specially designedMEA Packaged to reduce headspace and protect the productfrom lightMineral oilRELEASE SPECIFICATIONS2-cell MEA: 80% of control expanded toblastocysts at 72 hoursPeroxide toxicity MEA: Two-cell MEA 80% ofcontrol expanded to blastocysts at 72 hoursPeroxide value assay: 0.01 mEq/kgEndotoxins: 0.40 EU/mLShelf life: 20 weeks from date of manufactureSterile: Aseptically filteredSydney IVF Culture Oil protects gametes and embryosduring culture and IVF procedures by creating a barrierbetween the embryo culture medium and the atmosphere.This barrier helps to prevent evaporation and minimizesfluctuations in pH and osmolality.ReferencePart NumberK-SICO-50-2-AAVolumemL50 (x2)RELATED PRODUCTSSydney IVF Fertilization MediumSydney IVF Cleavage MediumSydney IVF Blastocyst MediumCook Flexipet ATIONSOLUTIONSUSAGEGAMETESOLUTIONS CONSTITUENTSBLASTOCYSTSOLUTIONSWe strongly recommend that you record the patient name and lot number every time you use Cook Medical IVF media in a clinical procedure.17
SYDNEY IVF FERTILIZATION MEDIUMSydney IVF Fertilization Medium is intended for use duringin vitro procedures for insemination and incubation ofoocytes.GAMETESOLUTIONS Bicarbonate buffered Can be used for both short and long inseminationprotocols Provides a glucose-rich environment for efficientcumulus-oocyte complex and sperm cell metabolism Includes antioxidants and nonessential amino acidsUSAGEFERTILIZATIONSOLUTIONSAfter the cumulus-oocyte complex has been washed,it is placed in Sydney IVF Fertilization Medium, whereinsemination occurs. The oocyte can remain in this mediumfor 16-18 hours. The fertilized oocytes are checked forpronuclei and then transferred into Sydney IVF CleavageMedium. This is the first step in the Cook Medical 7G46306G46305ReferencePart L2050100CONSTITUENTSCalcium lactateCalcium pantothenateD-glucoseEDTAGentamicinGlutamine, stabilizedGlycineHuman serum albumin (HSA)*L-alanineL-asparagineL-aspartic acidL-glutamic acidL-prolineL-serineMagnesium chlorideMagnesium sulphatePotassium chloridePotassium phosphatePurified waterSodium bicarbonateSodium chlorideSodium pyruvateTaurineRELEASE SPECIFICATIONSpH (in air): 7.5-7.8Osmolality: 285-295 mOsm/kg2-cell MEA: 80% of control expandedto blastocysts at 72 hoursEndotoxins: 0.4 EU/mLShelf life: 20 weeks from date of manufactureSterile: Aseptically filteredHSA assay: 2.50–6.25 mg/mLRELATED PRODUCTSBLASTOCYSTSOLUTIONSSydney IVF Gamete BufferSydney IVF Sperm MediumSydney IVF Cleavage MediumCook Flexipet PipettesWe strongly recommend that you record the patient name and lot number every time you use Cook Medical IVF media in a clinical procedure.*We take standard measures to prevent our medicinal human-blood and plasma products from causing infections. These steps include screening individual donationsand plasma pools for specific markers of infection and inactivating and removing viruses during manufacture. Despite these safeguards, a possibility exists that medicinalblood or plasma products will transmit infective agents, including unknown or emerging agents or other pathogens. No reports of proven virus transmission have beenlinked to albumin that was manufactured by established processes to European Pharmacopoeia specifications. We strongly recommend that you record the patient nameand lot number every time you use Cook Medical IVF media in a clinical procedure.18
SYDNEY IVF PVPSydney IVF PVP is intended for use as an aid in theimmobilization and isolation of individual spermcells prior to intracytoplasmic sperm injection (ICSI)procedures.Bicarbonate buffered Contains 10% polyvinylpyrrolidone (PVP)USAGESydney IVF PVP is used to reduce the motility ofsperm so that it is easier to isolate and capture withan ICSI pipette. The product should be equilibratedin 6% CO2 prior to use.ReferencePart NumberK-SIPV-200-5-AAVolumeμL200 (x5)RELATED PRODUCTSRELEASE SPECIFICATIONS2-cell MEA: 80% of control expandedto blastocysts at 72 hoursEndotoxins: 0.4 EU/mLShelf life: 20 weeks from date of manufactureSterile: Aseptically filteredHSA assay: 5.00–12.50 mg/mLCLEAVAGESOLUTIONSSydney IVF Gamete BufferSydney IVF Cleavage MediumCook Flexipet PipettesMicromanipulation cium lactateD-glucoseGentamicinGlutamine, stabilizedGlycineHuman serum albumin (HSA)*Magnesium sulphatePotassium chloridePotassium phosphatePurified waterPVP (MW 360,000)Sodium bicarbonateSodium chlorideSodium pyruvateTaurineGAMETESOLUTIONS CONSTITUENTSBLASTOCYSTSOLUTIONSWe strongly recommend that you record the patient name and lot number every time you use Cook Medical IVF media in a clinical procedure.*We take standard measures to prevent our medicinal human-blood and plasma products from causing infections. These steps include screening individual donationsand plasma pools for specific markers of infection and inactivating and removing viruses during manufacture. Despite these safeguards, a possibility exists that medicinalblood or plasma products will transmit infective agents, including unknown or emerging agents or other pathogens. No reports of proven virus transmission have beenlinked to albumin that was manufactured by established processes to European Pharmacopoeia specifications. We strongly recommend that you record the patient nameand lot number every time you use Cook Medical IVF media in a clinical procedure.19
CLEAVAGESOLUTIONSThe embryo's metabolic needs mustbe met for successful cleavage tooccur and for the embryo to beviable for transfer, cryopreservation,and further culturing. These mediaformulations meet those needs bydelivering necessary metabolites tothe developing embryo.21
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SYDNEY IVF CLEAVAGE MEDIUMSydney IVF Cleavage Medium is intended for useduring in vitro fertilization procedures for cultureand transfer of cleavage-stage embryos.A bicarbonate-buffered medium specificallydesigned for early cleavage-stage development Low in glucose and high in pyruvate to supportearly cleavage-stage development Recommended for ICSI procedures to supportoocytes when cumulus cell metabolism andsperm cell movement are no longer criticalUSAGEReferencePart Potassium chloridePotassiumphosphatePurified waterSodiumbicarbonateSodium chlorideSodium pyruvateTaurineRELEASE SPECIFICATIONSpH (in air): 7.5-7.8Osmolality: 285-295 mOsm/kg2-cell MEA: 80% of control expanded to blastocysts at 72 hoursEndotoxins: 0.4 EU/mLShelf life: 20 weeks from date ofmanufactureSterile: Aseptically filteredHSA assay: 2.50–6.25 mg/mLRELATED 6300L-aspartic acidL-cystineL-glutamic -tryptophanL-tyrosineFERTILIZATIONSOLUTIONSAfter normally fertilized oocytes are identified, theyare transferred into Sydney IVF Cleavage Mediumfor culture from day 1 to day 3 (up to the eight-cellstage). Sydney IVF Cleavage Medium is formulatedto provide early embryos with the necessarymetabolic substrates for development. This is thesecond step in the Cook Medical sequential system.Calcium lutamine,stabilizedGlycineHuman serumalbumin NS CONSTITUENTSMicromanipulation pipettesBLASTOCYSTSOLUTIONSWe strongly recommend that you record the patient name and lot number every time you use Cook Medical IVF media in a clinical procedure.*We take standard measures to prevent our medicinal human-blood and plasma products from causing infections. These steps include screening individual donationsand plasma pools for specific markers of infection and inactivating and removing viruses during manufacture. Despite these safeguards, a possibility exists that medicinalblood or plasma products will transmit infective agents, including unknown or emerging agents or other pathogens. No reports of proven virus transmission have beenlinked to albumin that was manufactured by established processes to European Pharmacopoeia specifications. We strongly recommend that you record the patient nameand lot number every time you use Cook Medical IVF media in a clinical procedure.23
BLASTOCYSTSOLUTIONSCellular differentiation is crucial forsuccessful IVF outcomes, and blastocystformation is a key indicator for embryotransfer. These blastocyst mediaformulations supply the nutrients thatare required to produce high-qualityblastocysts and to support themthrough uterine transfer andcryopreservation.The vitrification and warming kitscontain trehalose, ethylene glycol, andDMSO to remove and displace water,thereby reducing embryo exposure tocryoprotectants prior to vitrification.These flexible and convenient kits canbe used with any registered closedvitrification device that has a coolingrate greater than -15,000 ºC per minute.25
IONSBLASTOCYSTSOLUTIONS26
SYDNEY IVF BLASTOCYST MEDIUMSydney IVF Blastocyst Medium is intended for useduring in vitro fertilization procedures for extendedculture and transfer of embryos. Bicarbonate buffered Has a higher glucose concentration for blastocystmetabolism and energy production Includes essential and nonessential amino acidsto support blastocyst development Ideal for use in a low-oxygen environmentUSAGEOnce an embryo has reached day 3 (the eight-cell)stage, it is transferred into Sydney IVF BlastocystMedium, which is metabolically balanced to supportblastocyst development. This is the third and finalstep of the Cook Medical sequential system.ReferencePart NumberK-SIBM-20-AAK-SIBM-50-AAVolumemL2050RELATED PRODUCTSpH (in air): 7.5-7.8Osmolality: 280-290 mOsm/kg2-cell MEA: 80% of control expanded to blastocysts at 72 hoursEndotoxins: 0.4 EU/mLShelf life: 20 weeks from date of manufactureSterile: Aseptically filteredHSA assay: 2.50–6.25 mg/mLBLASTOCYSTSOLUTIONSEmbryo transfer cathetersSydney IVF Blastocyst Vitrification KitSydney IVF Blastocyst Warming KitCook Flexipet PipettesRELEASE threonineL-tryptophanL-tyrosineL-valineMagnesium chlorideMagnesium sulphatePotassium chloridePotassium phosphatePurified waterSodium bicarbonateSodium chlorideSodium pyruvateTaurineFERTILIZATIONSOLUTIONSDesigned to provide the necessary nutrientsto support blastulation, differentiation, andexpansionCalcium lactateCalcium pantothenateD-glucoseGentamicinGlutamine, stabilizedGlycineHuman serum albumin (HSA)*L-alanineL-arginineL-asparagineL-aspartic acidL-cystineL-glutamic SOLUTIONS CONSTITUENTSWe strongly recommend that you record the patient name and lot number every time you use Cook Medical IVF media in a clinical procedure.*We take standard measures to prevent our medicinal human-blood and plasma products from causing infections. These steps include screening individual donationsand plasma pools for specific markers of infection and inactivating and removing viruses during manufacture. Despite these safeguards, a possibility exists that medicinalblood or plasma products will transmit infective agents, including unknown or emerging agents or other pathogens. No reports of proven virus transmission have beenlinked to albumin that was manufactured by established processes to European Pharmacopoeia specifications. We strongly recommend that you record the patient nameand lot number every time you use Cook Medical IVF media in a clinical procedure.27
SYDNEY IVF BLASTOCYST VITRIFICATION KITThe Sydney IVF Blastocyst Vitrification Kit is intended tobe used for the vitrification of human blastocysts for ARTprocedures. This kit is designed for use with the Sydney IVFBlastocyst Warming Kit (K-SIBW-5000-AA).GAMETESOLUTIONS HEPES buffered Contains dimethyl sulfoxide (DMSO), ethylene glycol,and trehalose as cryoprotectants Used in a three-step vitrification process Not packaged ready to use—the user must combine thesolutionsFERTILIZATIONSOLUTIONSUSAGEThe Sydney IVF Blastocyst Vitrification Kit is used for thevitrification of blastocysts on day 5 or day 6. This HEPESbuffered kit contains DMSO, ethylene glycol, and trehaloseas cryoprotectants and has a three-step derNumberPart NumberG46292K-SIBV-5000-AARELATED PRODUCTSSydney IVF Blastocyst Warming KitSydney IVF Blastocyst MediumCook Flexipet PipettesEmbryo transfer cathetersVolumemL20 (x3)10 (x1)CONSTITUENTSCalcium lactateCalcium pantothenateD-glucoseDMSOEDTAEthylene glycolGentamicinGlutamine, stabilizedGlycineHEPESHuman serum albumin (HSA)*L-alanineL-asparagineL-aspartic acidL-glutamic acidL-prolineL-serineMagnesium chlorideMagnesium sulphatePotassium chloridePotassium phosphatePurified waterSodium bicarbonateSodium chlorideSodium pyruvateTrehaloseTaurineRELEASE SPECIFICATIONSpH (in air): 7.3-7.5Osmolality of base solution: 285-295 mOsm/kg2-cell MEA: 80% of control expanded toblastocysts at 72 hoursEndotoxins: 0.4 EU/mLShelf life: 20 weeks from date of manufactureSterile: Aseptically filteredHSA assay: 10.00–25.00 mg/mLBLASTOCYSTSOLUTIONSWe strongly recommend that you record the patient name and lot number every time you use Cook Medical IVF media in a clinical procedure.*We take standard measures to prevent our medicinal human-blood and plasma products from causing infections. These steps include screening individual donationsand plasma pools for specific markers of infection and inactivating and removing viruses during manufacture. Despite these safeguards, a possibility exists that medicinalblood or plasma products will transmit infective agents, including unknown or emerging agents or other pathogens. No reports of proven virus transmission have beenlinked to albumin that was manufactured by established processes to European Pharmacopoeia specifications. We strongly recommend that you record the patient nameand lot number every time you use Cook Medical IVF media in a clinical procedure.28
SYDNEY IVF BLASTOCYST WARMING KITThe Sydney IVF Blastocyst Warming Kit is intended forthe warming of human blastocysts that have undergonevitrification using the Sydney IVF Blastocyst Vitrification Kit(K-SIBV-5000-AA) for ART procedures.HEPES buffered Contains trehalose Used in a three-step warming processUSAGEThe Sydney IVF Blastocyst Warming Kit is used for thewarming of vitrified human blastocysts. This kit has a threestep warming and dilution protocol.ReferencePart NumberK-SIBW-5000-AAVolumemL20 (x3)RELATED PRODUCTSRELEASE SPECIFICATIONSpH (in air): 7.3-7.5Osmolality of base solution: 285-295 mOsm/kg2-cell MEA: 80% of control expanded toblastocysts at 72 hoursEndotoxins: 0.4 EU/mLShelf life: 20 weeks from date of manufactureSterile: Aseptically filteredHSA assay: 10.00–25.00 mg/mLCLEAVAGESOLUTIONSSydney IVF Blastocyst Vitrification KitSydney IVF Blastocyst MediumCook Flexipet PipettesEmbryo transfer cathetersL-prolineL-serineMagnesium chlorideMagnesium sulphatePotassium chloridePotassium phosphatePurified waterSodium bicarbonateSodium chlorideSodium rNumberG46291Calcium lactateCalcium pantothenateD-glucoseEDTAGentamicinGlutamine, stabilizedGlycineHEPESHuman serum albumin (HSA)*L-alanineL-asparagineL-aspartic acidL-glutamic acidGAMETESOLUTIONS CONSTITUENTSBLASTOCYSTSOLUTIONSWe strongly recommend that you record the patient name and lot number every time you use Cook Medical IVF media in a clinical procedure.*We take standard measures to prevent our medicinal human-blood and plasma products from causing infections. These steps include screening individual donationsand plasma pools for specific markers of infection and inactivating and removing viruses during manufacture. Despite these safeguards, a possibility exists that medicinalblood or plasma products will transmit infective agents, including unknown or emerging agents or other pathogens. No reports of proven virus transmission have beenlinked to albumin that was manufactured by established processes to European Pharmacopoeia specifications. We strongly recommend that you record the patient nameand lot number every time you use Cook Medical IVF media in a clinical procedure.29
COMPLETE LIST OF ORDER NUMBERSGAMETE SOLUTIONSOrderNumberReferencePart NumberFERTILIZATION SOLUTIONSVialVolumemLSydney IVF Gamete BufferOrderNumberReferencePart NumberVialVolumemLSydney IVF Culture OilG46320K-SIGB-20-AAG46318K-SIGB-50-AA5
vitro fertilization procedures to process sperm. D-glucoseUsed for sperm preparation and storage Recommended for swim-up and sperm washing in 6% CO 2 USAGE Sydney IVF Sperm Medium is designed for use with the swim-up technique to separate motile populations of sperm. Intrauterine insemination can be performed in this medium,
This plan addresses both Cook County and the Grand Portage Reservation. Grand Portage lies within the boundaries of Cook County, but is managed by the Tribe and is not subject to Cook County's administration. However, for the purposes of brevity, this plan will be referred to as the Cook County Comprehensive Trails Plan.
aray@cook.k12.ga.us Emily Dishman, Special Education Director Phone: 896-2296 edishman@cook.k12.ga.us Debra McNair, Alternative School Director Phone: 896-2294 dmcnair@cook.k12.ga.us Delane Purvis & Lee Browning, Transportation Phone: 549-6556 dpurvis@cook.k12.ga.us Patrina Fordham, School Nutrition Director Phone: 896-1891
Blood Culture 13 Manual Blood Culture Systems 13 Automated Blood Culture System 13 Culture System Veterinary 13 Miscellaneous 14 Transportation Swabs Aluminium Caps 14 Culture Media 15 Dehydrated Culture Media 15 Chromogenic Media 17 Supplementary Reagents 36 Antibiotic Single Supplements 37 Blood Products 37 Ready Poured Plates 38 .
4.1.1 The AIP — Cook Islands includes this manual and the AIP Supplement — Cook Islands. 4.1.2 Amendments to the AIP — Cook Islands are issued at regular intervals in accordance with the ICAO AIRAC schedule. The amendment cycle for the AIP — Cook Islands is shown in Table GEN 0.1 - 1 . Table GEN 0.1 - 1 Amendment Schedule
Thomas Cook is one of the largest tourism industry in Europe and UK. It aims to achieve the top-loved tourism industry, personalised holiday experiences, delivering inspiring for all its customers. . Thomas Cook, Cruise show, 2018 Figure 4. Thomas Cook, money exchange, 2018 . ECOFORUM [Volume 8, Issue 3(20), 2019] Figure 5. Thomas Cook .
1. Interpreting newspaper reports on the death of Cook 71 2. Early elegies 98 3. Anna Seward's Elegy on Captain Cook 110 4. Pictures of the death of Cook, 1779-1805 127 5. The lives and afterlives of John Webber's Death of Cook 148 Section 2 161 6.
Aerobic culture Anaerobic culture AFB culture Fungal culture Quantitative tissue culture (includes Aerobic culture) Deep wound culture (includes Aerobic and Anaerobic) Anaerobic Tissue Transport Media: PS59547 Tissue should sit on top of the media. Do Not Add Formalin. Tissue: (Any organ / solid tissue removed from the body i.e. kidney,
The University of Texas at Arlington Sequential Logic - Intro CSE 2340/2140 – Introduction to Digital Logic Dr. Gergely Záruba The Sequential Circuit Model x 1 Combinational z1 x n zm (a) y y Y Y Combinational logic logic x1 z1 x n z m Combinational logic with n inputs and m switching functions: Sequential logic with n inputs, m outputs, r .
