LABORATORY SAFETY MANUAL - SAHRIS SAHRA
Page 1 of 53Veterinary Tropical DiseasesFaculty of Veterinary ScienceDocument numberUniversity of PretoriaQM/QC/002Version 2 Copy 1Private Bag X04Onderstepoort0110Date IssuedSeptember 2011Republic of South AfricaLABORATORY SAFETY MANUALOF THEDepartment of Veterinary TropicalDiseasesFACULTY OF VETERINARYSCIENCEUNIVERSITY OF PRETORIACOPYRIGHTThis manual is the property of the Department of Veterinary Tropical Diseases and maynot be reproduced, copied or transmitted without the prior written consent of the Head ofDepartment and/or the Quality Manager.Name:Date:Compiled by:J GouwsSeptember 2011Reviewed by:E KappSeptember 2011Approved by:L TaljaardSeptember 2011
Page 2 of 53Veterinary Tropical DiseasesFaculty of Veterinary ScienceDocument numberUniversity of PretoriaQM/QC/002Version 2 Copy 1Private Bag X04Onderstepoort0110Date IssuedSeptember 2011Republic of South AfricaCONTENTS1.POLICY STATEMENT . 42.GENERAL SAFETY OF LABORATORY PERSONNEL . 53.LABORATORY PRECAUTIONS: . 64.FIRE HAZARD. 84.1.GENERAL CAUSES OF FIRES IN THE LABORATORY ARE: . 84.2.PREVENTATIVE MEASURES . 84.3.FIRE FIGHTING EQUIPMENT AND USE . 114.4.HANDLING OF FIRE FIGHTING EQUIPMENT: . 124.5.EMERGENCY PROCEDURE . 134.6.INSPECTION ROUTINE . 155.CHEMICAL HAZARDS . 165.1.INTRODUCTION. 165.2.SAFETY MEASURES FOR CHEMICALS . 185.3.INCOMPATIBLE CHEMICALS NOT TO BE STORED TOGETHER OR NEXT TO ANOTHER . 215.4.TOXIC EFFECT OF CHEMICAL SUBSTANCES . 255.5.CLEANING UP OF CHEMICAL SPILLS . 276.BIOSAFETY MEASURES . 326.1.ROUTES OF INFECTION . 33Name:Date:Compiled by:J GouwsSeptember 2011Reviewed by:E KappSeptember 2011Approved by:L TaljaardSeptember 2011
Page 3 of 53Veterinary Tropical DiseasesFaculty of Veterinary ScienceDocument numberUniversity of PretoriaQM/QC/002Version 2 Copy 1Private Bag X04Onderstepoort0110Date IssuedSeptember 2011Republic of South Africa6.2.CLASSIFICATION OF MICRO-ORGANISMS ON THE BASIS OF HAZARD . 346.3.CLASSIFICATION OF LABORATORIES ACCORDING TO HAZARD GROUP . 386.4.GENERAL PRECAUTIONS AGAINST LABORATORY-ACQUIRED INFECTIONS(BSL2 ) . 386.5.SPILLAGE AND BREAKAGE . 436.6.PRECAUTIONS AGAINST BLOOD-BORNE INFECTIONS. 446.7.STERILIZATION, DISINFECTION AND DECONTAMINATION . 456.8.DISPOSAL OF INFECTED WASTE . 486.9.CONCLUSIONS . 497.REFERENCES: . 498.FLOORPLAN OF THE DEPARTMENT OF VETERINARY TROPICAL DISEASES5010.CONFIRMATION LIST OF STAFF THAT HAVE READ THIS DOCUMENT . 53Name:Date:Compiled by:J GouwsSeptember 2011Reviewed by:E KappSeptember 2011Approved by:L TaljaardSeptember 2011
Page 4 of 53Veterinary Tropical DiseasesFaculty of Veterinary ScienceDocument numberUniversity of PretoriaQM/QC/002Version 2 Copy 1Private Bag X04Onderstepoort0110Date IssuedSeptember 2011Republic of South Africa1.POLICY STATEMENT1.The Department of Veterinary Tropical Diseases (DVTD) recognizes thatthe safety of all its employees and other persons on its premises is ofparamount importance and accepts its responsibility for providing a safeand healthy workplace. The DVTD will meet its responsibility as far as isachievable, paying particular attention to the provision and maintenanceof:I.a safe work place with safe access to and from the laboratoriesII.a healthy working environmentIII.equipment and systems which are safeIV.safe arrangements for the use, handling, storage and transport of articlesand substancesV.sufficient information, instruction, training and supervision to enableemployees to avoid hazards and contribute positively to their own healthand safety at work2.The DVTD undertakes the systematic identification of hazards, therecording of any significant risks arising from them, the establishment ofarrangements to eliminate, reduce or control risks and procedures for thereview and revision of these arrangements.Name:Date:Compiled by:J GouwsSeptember 2011Reviewed by:E KappSeptember 2011Approved by:L TaljaardSeptember 2011
Page 5 of 53Veterinary Tropical DiseasesFaculty of Veterinary ScienceDocument numberUniversity of PretoriaQM/QC/002Version 2 Copy 1Private Bag X04Onderstepoort0110Date IssuedSeptember 2011Republic of South Africa3.The DVTD will appoint safety officers competent to provide health andsafety advice and assistance to deal with emergencies and situations ofimminent danger to health and safety.4.The Faculty of Veterinary Science co-operates fully in the appointment of aLaboratory Safety Officers and will provide them where necessary, withsufficient facilities and training to carry out this task.5.The University of Pretoria ensures the existence of a Health and SafetyCommittee.6.A copy of this statement will be brought to the attention of all employees inthe DVTD. It will be reviewed or modified from time to time and may besupplemented in appropriate cases by further statements relating to thework of employees in the Department.2.GENERAL SAFETY OF LABORATORY PERSONNEL1.Eating, drinking and smoking are prohibited in the laboratory.2.Food must not be placed in a refrigerator used for specimens or reagents.3.Personal items such as handbags and spectacles must be kept away fromwork areas.4.Laboratory coats and gloves worn in the laboratory whilst processingspecimens should not be removed from the laboratory and should not beworn outside the laboratory area.5.Lab coats and gloves must be worn when handling all specimens.Name:Date:Compiled by:J GouwsSeptember 2011Reviewed by:E KappSeptember 2011Approved by:L TaljaardSeptember 2011
Page 6 of 53Veterinary Tropical DiseasesFaculty of Veterinary ScienceQM/QC/002Document numberVersion 2 Copy 1University of PretoriaPrivate Bag X04Onderstepoort0110Date IssuedSeptember 2011Republic of South Africa6.Safety glasses or a full-face shield must be worn when working withchemicals such as acids or cold liquids such as liquid nitrogen. Face/eyeprotection must also be worn when there is a risk of splashes.7.To avoid contamination, gloves should be removed when answering thetelephone, using the computer keyboard, opening the door etc.8.Hands must be washed efficiently with antiseptic soap after handlinginfectious specimens and prior to leaving the laboratory.9.All specimens processed in the laboratory must be handled as if they areinfectious in order to reduce the risk of transmission of infectiousorganisms.10. All staff should read this safety manual and sign that they have done so.The list of signatories is kept at the back of the safety manual, which iskept in the laboratory or the First Technologist’s office.11. Eye wash equipment (bottles) is kept in all laboratories. This must be usedin case of eye contact with any known or unknown liquid or substance.The distilled water in the bottles must be changed every 3 months and theexpiry date must be clearly written on the bottle.3.LABORATORY PRECAUTIONS:1.Specimens collected in syringes should have the needle removed or havethe needle capped before submitting the specimen to the laboratory.Name:Date:Compiled by:J GouwsSeptember 2011Reviewed by:E KappSeptember 2011Approved by:L TaljaardSeptember 2011
Page 7 of 53Veterinary Tropical DiseasesFaculty of Veterinary ScienceDocument numberUniversity of PretoriaQM/QC/002Version 2 Copy 1Private Bag X04Onderstepoort0110Date IssuedSeptember 2011Republic of South Africa2.Needles must be disposed of into a sharps container. Do not removeneedles by hand.3.Disposable pipettes are disposed of in a bio-hazard container.4.During centrifugation of specimens, the centrifuge cover is kept closeduntil the centrifuge comes to a complete stop.5.Specimens to be discarded are placed into bio-hazard bags and/or boxes.6.All contaminated disposable items used in the laboratory are placed in biohazard bags.7.Broken glass is disposed of into a sharps container.8.Work surfaces, counter tops and instruments are cleaned and disinfecteddaily, using a commercially purchased chlorine-based product orequivalent that is diluted with water. This dilution will depend on themanufacturer’s specifications and the purpose for which the disinfectantwill be used.9.All laboratory procedures should be performed according to the guidelinesin the safety manual and the relevant SOPs.10. Pipetting by mouth is strictly prohibited. Use a bulb when necessary or anautomatic dispensing pipette if available.11. Safety data sheets are available in the laboratory for all chemicals used inthe laboratory and for all chemicals incorporated into reagent mixtures. It isessential that all staff is aware of any potential dangers associated withthese chemicals and the safe handling thereof.Name:Date:Compiled by:J GouwsSeptember 2011Reviewed by:E KappSeptember 2011Approved by:L TaljaardSeptember 2011
Page 8 of 53Veterinary Tropical DiseasesFaculty of Veterinary ScienceDocument numberUniversity of PretoriaQM/QC/002Version 2 Copy 1Private Bag X04Onderstepoort0110Date IssuedSeptember 2011Republic of South Africa12. Disposable pipettes and/or disposable pipette tips are to be used whenpipetting corrosive and infective materials. These pipette tips and pipettesmust then be disposed of into bio-hazard bags/boxes or containers withantiseptic or disinfectant.13. The outside surface of the automatic dispensing pipette must be wipedusing a suitable disinfectant.4.FIRE HAZARD4.1.GENERAL CAUSES OF FIRES IN THE LABORATORY ARE:1.Electrical overloading2.Poor electrical maintenance3.Apparatus unnecessarily switched on (e.g. hot plates)4.Open flames (e.g. Bunsen burners)5.Inferior gas piping6.Recklessness with flammable chemicals/materials7.Flammable and explosive chemicals that are stored in refrigerators8.Matches and lighters4.2.PREVENTATIVE MEASURES4.2.1.Bunsen burners:1.Always turn off the gas when not in use or when the room is unoccupied.Name:Date:Compiled by:J GouwsSeptember 2011Reviewed by:E KappSeptember 2011Approved by:L TaljaardSeptember 2011
Page 9 of 53Veterinary Tropical DiseasesFaculty of Veterinary ScienceDocument numberUniversity of PretoriaQM/QC/002Version 2 Copy 1Private Bag X04Onderstepoort0110Date IssuedSeptember 2011Republic of South Africa2.Check the gas taps regularly for leaks with a weak soap solution.3.Report any gas leaks immediately to the official in charge of maintenanceafter the gas to the room concerned has been turned off. Personnel mustbe informed of the location of the gas line stop valves. The latter should beclearly marked.4.Tubing to Bunsen burners must by inspected regularly and replaced wherenecessary.5.Do not handle flammable substances near an open flame. Make sure thatbottles containing volatile chemicals are tightly closed.4.2.2.Gas cylinders1.Do not place gas cylinders near heat emitting sources.2.Keep the number of gas cylinders (with explosive gases) to the minimum.3.Gas cylinders must be secured to the wall or cylinder stand if located inthe laboratory.4.It is against regulations to have butane/propane gas cylinders inside alaboratory. These should be stored in designated locked gas cagesoutside the building.4.2.3.Flammable chemicals and strong acidsName:Date:Compiled by:J GouwsSeptember 2011Reviewed by:E KappSeptember 2011Approved by:L TaljaardSeptember 2011
Page 10 of 53Veterinary Tropical DiseasesFaculty of Veterinary ScienceQM/QC/002Document numberVersion 2 Copy 1University of PretoriaPrivate Bag X04Onderstepoort0110Date IssuedSeptember 2011Republic of South Africa1.Store flammable chemicals and strong acids in volumes not exceeding 5litres in a fire proof cabinet or room. Examples are ether, benzene,tolueen, xylol, acetone and alcohol. Always handle with care.2.Information about the chemicals is available in the laboratories chemicalfile and/or on the internet website www.sciencelab.com/msdsList.php.3.4.2.4.Flammable chemicals and strong acids should be stored separately.Electrical apparatus /wiring1.Defective electrical apparatus should be reported immediately. Only aqualified electrician must carry out electrical repairs.2.Do not overload the electrical current by plugging in more than oneappliance to a power point.3.Switch off heating apparatus when not in use, e.g. hot plates.4.Worn electrical cords of apparatus must be replaced.5.All electrical cords must be secured.6.Document incidents of short circuits in the electrical system and have thesystem checked regularly by the maintenance contractor.4.2.5.Smoking in any of the laboratories is prohibited.4.2.6.Rooms and passages must be kept free of litter. Prevent the accumulation ofrubbish in the building.Name:Date:Compiled by:J GouwsSeptember 2011Reviewed by:E KappSeptember 2011Approved by:L TaljaardSeptember 2011
Page 11 of 53Veterinary Tropical DiseasesFaculty of Veterinary ScienceDocument numberUniversity of PretoriaQM/QC/002Version 2 Copy 1Private Bag X04Onderstepoort0110Date IssuedSeptember 2011Republic of South Africa4.3.FIRE FIGHTING EQUIPMENT AND USEThe following is available in the Department:NumberFire hose reels5Location1 North wing passage at lift1 East wing passage at dept. safeNearest fire hose reels1 East wing passage near HOD office1 South wing passage at entrance1 West wing passage next to 2-41Fire extinguishers (dry10In all the passagespowder)Asbestos blanketsNoneAsbestos gloves2At liquid nitrogen systemThese items must never be removed unless required for a fire!ClassABType or fireCombustibleExampleWood, paper,substancesmaterialsFlammable liquidsAlcohol, ether, etc.RecommendedWater, Dry powderCO2, Dry powderExtinguisherCElectrical short circuitsCO2 , Dry powderExtinguisherName:Date:Compiled by:J GouwsSeptember 2011Reviewed by:E KappSeptember 2011Approved by:L TaljaardSeptember 2011
Page 12 of 53Veterinary Tropical DiseasesFaculty of Veterinary ScienceDocument numberUniversity of PretoriaQM/QC/002Version 2 Copy 1Private Bag X04Onderstepoort0110Date IssuedSeptember 2011Republic of South Africa4.4.HANDLING OF FIRE FIGHTING EQUIPMENT:4.4.1.Fire hose reelsNB. Water is a good conductor of electricity and the person handling the firehose could receive a fatal electric shock. Turn the main electricity supply off.1.Unroll the fire hose.2.Open the stopcock of the water mains.3.Make sure that there is a water supply (by means of the control tap on thenozzle) before running to the fire.4.Direct the nozzle to the base of the fire and open the control valve.5.After the fire has been extinguished, close the tap and neatly roll up thefire hose so that it is ready for use again.4.4.2.Carbon dioxide or dry powder fire extinguishersCO2 or dry powder extinguishers are especially useful in extinguishing smallfires caused by flammable chemicals and electrical shorts. It does not causepollution and can be safely used on burning apparatus.1.Remove pin.2.Direct the nozzle at the base of the flames.3.Spray the fire after squeezing the lever fully open with the thumb.Name:Date:Compiled by:J GouwsSeptember 2011Reviewed by:E KappSeptember 2011Approved by:L TaljaardSeptember 2011
Page 13 of 53Veterinary Tropical DiseasesFaculty of Veterinary ScienceDocument numberUniversity of PretoriaQM/QC/002Version 2 Copy 1Private Bag X04Onderstepoort0110Date IssuedSeptember 2011Republic of South Africa4.4.3.Asbestos glovesThe gloves are specifically for the use of those handling the fire fightingequipment.4.4.4.Asbestos blanketThe blanket is used to wrap around a person who is on fire or to cover burningmaterial.Showers are available at the chemical store to extinguish burning clothes.4.5.EMERGENCY PROCEDURE4.5.1.Small fires4.5.2.1.Use the dry powder fire extinguishers.2.Raise alarm in the building and call for help.3.If the fire cannot be extinguished follow the steps under Big fires4.Avoid crowding of onlookers.Big firesIt includes fires that also originated in other parts of the building1.Raise alarm immediately.2.Do not contact the emergency services yourself.Name:Date:Compiled by:J GouwsSeptember 2011Reviewed by:E KappSeptember 2011Approved by:L TaljaardSeptember 2011
Page 14 of 53Veterinary Tropical DiseasesFaculty of Veterinary ScienceQM/QC/002Document numberVersion 2 Copy 1University of PretoriaPrivate Bag X04Onderstepoort0110Date IssuedSeptember 2011Republic of South Africa3.Dial 012 4202310 and inform the emergency section of the location, extentand nature of the fire. The fire brigade will be summoned.4.Emergency numbers must be visible in every laboratory.5.Close all doors and windows. Keep them closed!!!6.Breathe through a wet handkerchief/towel and crawl low down on the floorwhen smoke is present.7.Evacuate the area and use the fire escape (west wing i.e. Serology), ifpossible to leave the building.8.Do not panic and avoid crowding at the exits.9.If trapped, attract attention from the windows. Hose down all those trappedwith water.10. Members of the fire team and management of the department will see to:I.Turning off of the main gas supply in gas cage.II.Switching off of the main electricity supply to the department.III.The evacuation of and first aid to the injured.IV.Communication aspects.Note:The telephone might not be working and help must be sought by other means.As a result of the lack of electricity and smoke, passages may be shrouded indarkness. Torches and a chopper stored in the fire reel cabinet may be of use.Name:Date:Compiled by:J GouwsSeptember 2011Reviewed by:E KappSeptember 2011Approved by:L TaljaardSeptember 2011
Page 15 of 53Veterinary Tropical DiseasesFaculty of Veterinary ScienceDocument numberUniversity of PretoriaQM/QC/002Version 2 Copy 1Private Bag X04Onderstepoort0110Date IssuedSeptember 2011Republic of South Africa4.6.INSPECTION ROUTINEElectrical equipment, e.g. wiring, power points, etc.- 2 monthlyGas tubing to Bunsen burners- 2 monthlyFire fighting equipment- annuallyREFERENCES:Laboratory Biosafety Manual, Second Edition, WHOName:Date:Compiled by:J GouwsSeptember 2011Reviewed by:E KappSeptember 2011Approved by:L TaljaardSeptember 2011
Page 16 of 53Veterinary Tropical DiseasesFaculty of Veterinary ScienceQM/QC/002Document numberVersion 2 Copy 1University of PretoriaPrivate Bag X04Onderstepoort0110Date IssuedSeptember 2011Republic of South Africa5.CHEMICAL HAZARDS5.1.INTRODUCTIONAll chemicals must be regarded as potentially dangerous.International Hazard warning symbols are clearly indicated on the label of mostchemicals (example follows). Sometimes the directions for safe storage are alsoindicated. These symbols are:5.1.1.Oxidising:Reagents that cause highly exothermic reactions when it comes into able chemicals that can explode when they dry out or when exposed tofriction, shock or heat e.g. ammonium dichromate5.1.3.Flammable:I.Chemicals with a flash point of under 66ºC (150ºF).II.Chemicals that spontaneously ignite, e.g. phosphorus.Name:Date:Compiled by:J GouwsSeptember 2011Reviewed by:E KappSeptember 2011Approved by:L TaljaardSeptember 2011
Page 17 of 53Veterinary Tropical DiseasesFaculty of Veterinary ScienceDocument numberUniversity of PretoriaQM/QC/002Version 2 Copy 1Private Bag X04Onderstepoort0110Date IssuedSeptember 2011Republic of South AfricaIII.Chemicals that give off flammable gases when in contact with water, e.g.lithium.5.1.4.Toxic:Chemicals with a serious danger of acute poisoning, irrespective of the waythey come in contact with the body I.e. inhalation, swallowing or skin contact.5.1.5.Caustic (Corrosive):Chemicals that can destroy living tissue or damage apparatus e.g. sulphuricacid5.1.6.Irritant:Chemicals with an irritant or harmful effects on the skin, eyes and respiratoryorgans, e.g. ammonia.5.1.7.Radio-active:Chemicals with a radiation risk.Name:Date:Compiled by:J GouwsSeptember 2011Reviewed by:E KappSeptember 2011Approved by:L TaljaardSeptember 2011
Page 18 of 53Veterinary Tropical DiseasesFaculty of Veterinary ScienceDocument numberUniversity of PretoriaQM/QC/002Version 2 Copy 1Private Bag X04Onderstepoort0110Date IssuedSeptember 2011Republic of South Africa5.2.SAFETY MEASURES FOR CHEMICALSAlways read the label on the container and or MSDS, making sure that all theinstructions with regards to the use of the chemical are strictly adhered to5.2.1.Storage1.Chemicals should be stored according to instructions on the label and thematerial safety data sheets2.Store in a well ventilated room, preferably at room temperature, protectedagainst big temperature fluctuations.3.All chemicals should be store in a controlled environment, whereunauthorised entrance is prohibited. Poisonous chemicals e.g. potassiumcyanide must be kept under strict control e.g. locked and a register mustbe signed with full details of its use.4.Organic solvents, e.g. ethyl alcohol must be kept in a lockable cabinet andseparate from acids. Smoking is prohibited where flammable chemicalsare stored.5.Oxidative acids must be stored in a well-ventilated room, as close aspossible to the floor, where the containers cannot spill unto otherchemicals when accidentally knocked over.Name:Date:Compiled by:J GouwsSeptember 2011Reviewed by:E KappSeptember 2011Approved by:L TaljaardSeptember 2011
Page 19 of 53Veterinary Tropical DiseasesFaculty of Veterinary ScienceDocument numberUniversity of PretoriaQM/QC/002Version 2 Copy 1Private Bag X04Onderstepoort0110Date IssuedSeptember 2011Republic of South Africa6.Some chemicals must not be stored in the fridge as there is a danger thatthey will ignite when the fridge switches on. Others chemicals e.g. picricacid can explode on contact with moisture.5.2.2.Handling:1.Check the identity of the chemical in the container.2.Wear protective clothing and eye or face protection where necessary.3.Open container carefully in a well-ventilated area or in a fume cabinet.4.Guard against ingestion, inhalation and contact with the skin, eyes andclothes. Never pipette by mouth.5.Eating, drinking or smoking is prohibited, when working with chemicals.6.Take precautions against static electricity and avoid friction by not joltingcontainers.7.Do not add water carelessly, especially when using acids.8.Add acid to water and not the other way around.9.Some chemicals, e.g. sodium hydroxide react exothermically when wateris added to it. Such flasks may become very hot.10. Use appropriate procedures to clean up spilt chemicals. Refer to 5.5 inSafety manual and appropriate MSDS.Name:Date:Compiled by:J GouwsSeptember 2011Reviewed by:E KappSeptember 2011Approved by:L TaljaardSeptember 2011
Page 20 of 53Veterinary Tropical DiseasesFaculty of Veterinary ScienceDocument numberUniversity of PretoriaQM/QC/002Version 2 Copy 1Private Bag X04Onderstepoort0110Date IssuedSeptember 2011Republic of South Africa11. Should exposure to a chemical substance affect you in any way,immediately seek first aid until medical assistance is available.12. Take note of the first aid box in the passage or media room, as well as thewall chart indicating the relevant information about chemicals.Name:Date:Compiled by:J GouwsSeptember 2011Reviewed by:E KappSeptember 2011Approved by:L TaljaardSeptember 2011
Page 21 of 53Veterinary Tropical DiseasesFaculty of Veterinary ScienceQM/QC/002Document numberVersion 2 Copy 1University of PretoriaPrivate Bag X04Onderstepoort0110Date IssuedSeptember 2011Republic of South Africa5.3.INCOMPATIBLE CHEMICALS NOT TO BE STORED TOGETHEROR NEXT TO ANOTHERCHEMICALINCOMPATIBILITYChromic acid, nitric acid, hydroxyl compounds, ethyleneAcetic acidglycol, perchloric acid, peroxides, permanganatesAcetoneConcentrated nitric and sulfuric acid mixturesAcetyleneChlorine, bromine, copper, fluorine, silver, mercuryAlkali and alkaline earthWater, carbon tetrachloride or other chlorinatedmetals (lithium, sodium,hydrocarbons, carbon dioxide, halogens, powdered metalspotassium)(e.g., aluminum or magnesium)Mercury (e.g., in manometers), chlorine, calciumAmmonia(anhydrous)hypochlorite, iodine, bromine, hydrofluoric acid (anhydrous)Acids, powdered metals, flammable liquids, chlorates,Ammonium nitratenitrates, sulfur, finely divided organic or combustiblematerialsAnilineNitric acid, hydrogen peroxideArsenical materialsAny reducing agentAzidesAcidsBromineSee ChlorineCalcium oxideWaterCarbon (activated)Calcium hypochlorite, all oxidizing agentsName:Date:Compiled by:J GouwsSeptember 2011Reviewed by:E KappSeptember 2011Approved by:L TaljaardSeptember 2011
Page 22 of 53Veterinary Tropical DiseasesFaculty of Veterinary ScienceDocument numberUniversity of PretoriaQM/QC/002Version 2 Copy 1Private Bag X04Onderstepoort0110Date IssuedSeptember 2011Republic of South AfricaCHEMICALINCOMPATIBILITYSodium, Chlorates, Ammonium salts, acids, powderedCarbon tetrachloridemetals, sulfur, finely divided organic or combustiblematerialsAmmonia, acetylene, butadiene, butane, methane,Chlorinepropane (or other petroleum gases), hydrogen, sodiumcarbide, benzene, finely divided metals, turpentineChlorine dioxideAmmonia, methane, phosphine, hydrogen sulfideChromic acid and chromiumAcetic acid, naphthalene, camphor, glycerol, alcohol,flammable liquids in generalCopperAcetylene, hydrogen peroxideCumene hydro peroxideAcids (organic or inorganic)CyanidesAcidsAmmonium nitrate, chromatic acid, hydrogen peroxide,Flammable liquidsnitric acid, sodium peroxide, halogensFluorineIsolate from e, chlorine, bromine, chromic acid, sodium peroxidebenzene)Hydrocyanic acidNitric acid, alkaliHydrofluoric acidAmmonia (aqueous or anhydrous)(anhydrous)Name:Date:Compiled by:J GouwsSeptember 2011Reviewed by:E KappSeptember 2011Approved by:L TaljaardSeptember 2011
Page 23 of 53Veterinary Tropical DiseasesFaculty of Veterinary ScienceDocument numberUniversity of PretoriaQM/QC/002Version 2 Copy 1Private Bag X04Onderstepoort0110Date IssuedSeptember 2011Republic of South AfricaCHEMICALINCOMPATIBILITYCopper, chromium, iron, most metals or their salts,Hydrogen peroxidealcohols, acetone, organic materials, aniline, nitromethane, combustible materialsHydrogen sulfideFuming nitric acid, oxidizing gasesHypochloriteAcids, activated carbonIodineAcetylene, ammonia (aqueous or anhydrous), hydrogenMercuryAcetylene, fulminic acid, ammoniaNitratesSulfuric acidAcetic acid, aniline, chromic acid, hydrocyanic acid,Nitric acid (concentrated)hydrogen sulfide, flammable liquids, flammable gases,copper, brass, any heavy metalsNitritesPotassium or sodium cyanide.Nitro-paraffinInorganic bases, aminesOxalic acidSilver, mercuryOxygenOils, grease, hydrogen, flammable: liquids, solids, or gasesAcetic anhydride, bismuth and its alloys, alcohol, paper,Perchloric acidwood, grease, oilsPeroxides, OrganicAcids (organic or mineral), avoid friction, store coldPhosphorus (white)Air, oxygen, alkalis, reducing agentsPhosphorus pentoxideWaterName:Date:Compiled by:J GouwsSeptember 2011Reviewed by:E KappSeptember 2011Approved by:L TaljaardSeptember 2011
Page 24 of 53Veterinary Tropical DiseasesFaculty of Veterinary ScienceDocument numberUniversity of PretoriaQM/QC/002Version 2 Copy 1Private Bag X04Onderstepoort0110Date IssuedSeptember 2011Republic of South AfricaCHEMICALINCOMPATIBILITYPotassiumCarbon tetrachloride, carbon dioxide, waterPotassium chlorateSulfuric and other acidsPotassium perchlorate(see Sulfuric and other acids also chlorates)Potassium permanganateGlycerol, ethylene glycol, benzaldehyde, sulfuric acidSelenidesReducing agentsAcetylene, oxalic acid, tartaric acid, ammoniumSilvercompounds, fulminic acidSodiumCarbon tetrachloride, carbon dioxide, waterSodium ChlorateAcids, ammonium salts, oxidizable materials, sulfurSodium nitriteAmmonium nitrate and other ammonium saltsEthyl or methyl alcohol, glacial acetic acid, aceticSodium peroxideanhydride, benzaldehyde, carbon disulfide, glycerin,ethylene glycol, ethyl acetate, methyl acetate, furfuralSulfidesAcidsPotassium chlorate, potassium perchlorate, potassiumSulfuric acidpermanganate (similar compounds of light metals, such assodium, lithium)TellurideReducing agentsAcetyl chloride, alkaline and alkaline earth metals, theirWaterhydrides and oxides, barium peroxide, carbides, chromicacid, phosphorous oxychloride, phosphorousName:Date:Compiled by:J GouwsSeptember 2011Reviewed by:E KappSeptember 2011Approved by:L TaljaardSeptember 2011
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4.2.2. Gas cylinders 1. Do not place gas cylinders near heat emitting sources. 2. Keep the number of gas cylinders (with explosive gases) to the minimum. 3. Gas cylinders must be secured to the wall or cylinder stand if located in the laboratory. 4. It is against regulations to have butane/pr
UTRGV Laboratory Safety Manual 8 Laboratory Safety March 2016 A. INTRODUCTION Note to Users of This Laboratory Safety Manual The Department of Environmental Health, Safety and Risk Management (EHSRM) has prepared this manual to ensure that laboratory activities are conducted in compliance with
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